Restriction map for
pFN21AF0007
(Circular) (Six-base) MAPSORT of: pFN21AF0007.seq Check: 5700 from: 1 to: 6775
pFN21AF0007 6775 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2356 3913 2356
Size: 1557 5218
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4585 278
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4118 1937
Size: 2181 4594
Acc65I G'GTAC_C
Cuts at: 4096 4096
Size: 6775
AclI AA'CG_TT
Cuts at: 1563 4902 5275 1563
Size: 3339 373 3063
Fragments arranged by size:
3339 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2219 2849 2873 3069 3245 4838 6222
Size: 431 156 630 24 196 176 1593 1384
Cuts at: 6222 6490 1632
Size: 268 1917
Fragments arranged by size:
1917 1593 1384 630 431 268 196 176
156 24
AfeI AGC'GCT
Cuts at: 2996 3218 2996
Size: 222 6553
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6578
AflIII A'CryG_T
Cuts at: 1203 2470 3424 5674 6651 1203
Size: 1267 954 2250 977 1327
Fragments arranged by size:
2250 1327 1267 977 954
AgeI A'CCGG_T
Cuts at: 5606 5606
Size: 6775
AhdI GACnn_n'nnGTC
Cuts at: 5504 5504
Size: 6775
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6775
AlwNI CAG_nnn'CTG
Cuts at: 2182 2569 2614 2921 3664 3871 4518 6090 2182
Size: 387 45 307 743 207 647 1572 2867
Fragments arranged by size:
2867 1572 743 647 387 307 207 45
ApaI G_GGCC'C
Cuts at: 3409 3742 3933 3409
Size: 333 191 6251
Fragments arranged by size:
6251 333 191
ApaLI G'TGCA_C
Cuts at: 4832 5988 4832
Size: 1156 5619
ApoI r'AATT_y
Cuts at: 1493 3776 4084 4310 4462 6571 1493
Size: 2283 308 226 152 2109 1697
Fragments arranged by size:
2283 2109 1697 308 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5329 160
Size: 872 4297 1606
Fragments arranged by size:
4297 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
AvaI C'yCGr_G
Cuts at: 1870 1943 3735 4100 1870
Size: 73 1792 365 4545
Fragments arranged by size:
4545 1792 365 73
BamHI G'GATC_C
Cuts at: 4048 4105 4472 4048
Size: 57 367 6351
Fragments arranged by size:
6351 367 57
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 2255 3994 4096 5551
Size: 325 217 627 424 44 1739 102 1455
Cuts at: 5551 618
Size: 1842
Fragments arranged by size:
1842 1739 1455 627 424 325 217 102
44
BanII G_rGCy'C
Cuts at: 1391 3369 3409 3642 3742 3933 4094 1391
Size: 1978 40 233 100 191 161 4072
Fragments arranged by size:
4072 1978 233 191 161 100 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3790 928
Size: 971 1891 3913
Fragments arranged by size:
3913 1891 971
BbvCI CC'TCA_GC
Cuts at: 2409 2898 3318 2409
Size: 489 420 5866
Fragments arranged by size:
5866 489 420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4966 5000 1903
Size: 34 50 34 2945 34 3678
Fragments arranged by size:
3678 2945 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4667 5877 4667
Size: 1210 5565
BclI T'GATC_A
Cuts at: 1548 2923 1548
Size: 1375 5400
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2285 3182 3404
Size: 107 122 71 631 605 613 897 222
Cuts at: 3404 5386 136
Size: 1982 1525
Fragments arranged by size:
1982 1525 897 631 613 605 222 122
107 71
BglII A'GATC_T
Cuts at: 6770 6770
Size: 6775
BlpI GC'TnA_GC
Cuts at: 2069 2435 3327 3698 4192 2069
Size: 366 892 371 494 4652
Fragments arranged by size:
4652 892 494 371 366
Bme1580I G_kGCm'C
Cuts at: 1790 2214 3151 3409 3718 3742 3933 4836 5992
Size: 424 937 258 309 24 191 903 1156
Cuts at: 5992 1790
Size: 2573
Fragments arranged by size:
2573 1156 937 903 424 309 258 191
24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2584 2593 4506
Size: 416 53 83 399 24 1167 9 1913
Cuts at: 4506 5464 442
Size: 958 1753
Fragments arranged by size:
1913 1753 1167 958 416 399 83 53
24 9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2159 2291 2378 2546 2816 2960 2999
Size: 893 27 132 87 168 270 144 39
Cuts at: 2999 3083 3509 3787 5435 1239
Size: 84 426 278 1648 2579
Fragments arranged by size:
2579 1648 893 426 278 270 168 144
132 87 84 39 27
Bpu10I CC'TnA_GC
Cuts at: 2372 2409 2759 2898 3318 3564 2372
Size: 37 350 139 420 246 5583
Fragments arranged by size:
5583 420 350 246 139 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 2758 4900 5765 6063 2690
Size: 68 2142 865 298 3402
Fragments arranged by size:
3402 2142 865 298 68
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5438 882
Size: 617 3939 2219
Fragments arranged by size:
3939 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3427 4566 493
Size: 711 2223 1139 2702
Fragments arranged by size:
2702 2223 1139 711
BsaBI GATnn'nnATC
Cuts at: 6769 6769
Size: 6775
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2312 4582 4964 5599
Size: 53 83 186 936 779 2270 382 635
Cuts at: 5599 275
Size: 1451
Fragments arranged by size:
2270 1451 936 779 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2214 3677 5208 5606 5880 6027 6438 2214
Size: 1463 1531 398 274 147 411 2551
Fragments arranged by size:
2551 1531 1463 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3467 3497 1178
Size: 30 165 30 2064 30 4456
Fragments arranged by size:
4456 2064 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3368 3389 3515 3707 3876 1186
Size: 2182 21 126 192 169 4085
Fragments arranged by size:
4085 2182 192 169 126 21
BseYI C'CCAG_C
Cuts at: 2288 2607 2838 3890 5978 6474 2288
Size: 319 231 1052 2088 496 2589
Fragments arranged by size:
2589 2088 1052 496 319 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3405 3708 3891 3921 1163
Size: 573 1669 303 183 30 4017
Fragments arranged by size:
4017 1669 573 303 183 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4225 4986 6014 1540
Size: 275 186 2224 761 1028 2301
Fragments arranged by size:
2301 2224 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2576 2736 3369 4836 4921 5992 2576
Size: 160 633 1467 85 1071 3359
Fragments arranged by size:
3359 1467 1071 633 160 85
BsmI GAATG_Cn'
Cuts at: 3647 4286 4379 3647
Size: 639 93 6043
Fragments arranged by size:
6043 639 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3350 3350
Size: 6775
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2576 2736 3151 3369 3409 3642
Size: 399 424 362 160 415 218 40 233
Cuts at: 3642 3718 3742 3933 4094 4836 4921 5992 1391
Size: 76 24 191 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 415 399 362 233
218 191 161 160 85 76 40 24
BspEI T'CCGG_A
Cuts at: 6438 6438
Size: 6775
BspHI T'CATG_A
Cuts at: 4664 6394 4664
Size: 1730 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2356 2646 3913 4130 844
Size: 1512 290 1267 217 3489
Fragments arranged by size:
3489 1512 1267 290 217
BsrBI CCG'CTC
Cuts at: 1394 1823 2220 3091 3268 4662 1394
Size: 429 397 871 177 1394 3507
Fragments arranged by size:
3507 1394 871 429 397 177
BsrDI GCAATG_nn'
Cuts at: 65 1248 2635 5270 5444 65
Size: 1183 1387 2635 174 1396
Fragments arranged by size:
2635 1396 1387 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 4033 5419 5606 1700
Size: 110 222 2001 1386 187 2869
Fragments arranged by size:
2869 2001 1386 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 3666 96
Size: 3570 3205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6775
BssSI C'ACGA_G
Cuts at: 3400 4835 5847 3400
Size: 1435 1012 4328
Fragments arranged by size:
4328 1435 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6775
BstBI TT'CG_AA
Cuts at: 5579 5579
Size: 6775
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6775
BstXI CCAn_nnnn'nTGG
Cuts at: 3038 3038
Size: 6775
BstYI r'GATC_y
Cuts at: 1972 2104 2123 2538 3027 4048 4063 4105 4472
Size: 132 19 415 489 1021 15 42 367
Cuts at: 4472 4857 4874 6326 6412 6424 6770 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1021 489 415 385 367 346
132 86 42 19 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 3708 3708
Size: 6775
BtgI C'CryG_G
Cuts at: 513 1065 2283 2533 2809 3037 513
Size: 552 1218 250 276 228 4251
Fragments arranged by size:
4251 1218 552 276 250 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6036
Fragments arranged by size:
6036 683 56
BtsI GCAGTG_nn'
Cuts at: 4291 5085 5105 4291
Size: 794 20 5961
Fragments arranged by size:
5961 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6775
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6740
DraI TTT'AAA
Cuts at: 4079 4079
Size: 6775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3521 5782 809
Size: 507 2205 2261 1802
Fragments arranged by size:
2261 2205 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3396 4054 4222
Size: 54 1425 50 275 18 1566 658 168
Cuts at: 4222 5111 6740 8
Size: 889 1629 43
Fragments arranged by size:
1629 1566 1425 889 658 275 168 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4222 1537
Size: 275 2410 4090
Fragments arranged by size:
4090 2410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 3813 4705 1353
Size: 842 1618 892 3423
Fragments arranged by size:
3423 1618 892 842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 2365 2575 3131 5362 5736
Size: 1064 138 528 248 210 556 2231 374
Cuts at: 5736 5882 387
Size: 146 1280
Fragments arranged by size:
2231 1280 1064 556 528 374 248 210
146 138
EcoICRI GAG'CTC
Cuts at: 3367 3367
Size: 6775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2132 2159 2219 2291 2378 2546
Size: 393 431 69 27 60 72 87 168
Cuts at: 2546 2816 2849 2873 2960 2999 3069 3083 3245
Size: 270 33 24 87 39 70 14 162
Cuts at: 3245 3509 3787 4838 5435 6222 6490 1239
Size: 264 278 1051 597 787 268 1524
Fragments arranged by size:
1524 1051 787 597 431 393 278 270
268 264 168 162 87 87 72 70
69 60 39 33 27 24 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3191 3656 3191
Size: 465 6310
EcoO109I rG'GnC_Cy
Cuts at: 2710 2743 3301 3405 3406 3661 3930 2710
Size: 33 558 104 1 255 269 5555
Fragments arranged by size:
5555 558 269 255 104 33 1
EcoRI G'AATT_C
Cuts at: 4084 4462 4084
Size: 378 6397
EcoRV GAT'ATC
Cuts at: 3572 3572
Size: 6775
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 3842 2071
Size: 1771 5004
FspI TGC'GCA
Cuts at: 5281 5281
Size: 6775
HaeII r_GCGC'y
Cuts at: 1678 2266 2358 2998 3220 5922 1678
Size: 588 92 640 222 2702 2531
Fragments arranged by size:
2702 2531 640 588 222 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2428 2460 3123
Size: 32 135 32 536 32 288 32 663
Cuts at: 3123 3155 3982 4014 5416 5448 5490 5522 1373
Size: 32 827 32 1402 32 42 32 2626
Fragments arranged by size:
2626 1402 827 663 536 288 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4119 4365 669
Size: 988 281 2181 246 3079
Fragments arranged by size:
3079 2181 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2048 748
Size: 1300 5475
HpaI GTT'AAC
Cuts at: 4365 4365
Size: 6775
KpnI G_GTAC'C
Cuts at: 4100 4100
Size: 6775
MfeI C'AATT_G
Cuts at: 4374 4374
Size: 6775
MluI A'CGCG_T
Cuts at: 6651 6651
Size: 6775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2426 3335 3903 5889 6073 1305
Size: 1121 909 568 1986 184 2007
Fragments arranged by size:
2007 1986 1121 909 568 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3398 4056 10
Size: 54 1425 343 1566 658 2729
Fragments arranged by size:
2729 1566 1425 658 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4733 5092 5251 518
Size: 648 3567 359 159 2042
Fragments arranged by size:
3567 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2464 2614 2811 3317 4191 4868 6016
Size: 462 396 150 197 506 874 677 1148
Cuts at: 6016 6261 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 874 677 506 462 396 245
197 150
NaeI GCC'GGC
Cuts at: 2034 4035 2034
Size: 2001 4774
NcoI C'CATG_G
Cuts at: 513 1065 2533 3037 513
Size: 552 1468 504 4251
Fragments arranged by size:
4251 1468 552 504
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6775
NgoMIV G'CCGG_C
Cuts at: 2032 4033 2032
Size: 2001 4774
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6775
NotI GC'GGCC_GC
Cuts at: 4222 4222
Size: 6775
NspI r_CATG'y
Cuts at: 1132 1229 2804 4133 5678 1132
Size: 97 1575 1329 1545 2229
Fragments arranged by size:
2229 1575 1545 1329 97
PasI CC'CwG_GG
Cuts at: 1348 3836 3974 1348
Size: 2488 138 4149
Fragments arranged by size:
4149 2488 138
PciI A'CATG_T
Cuts at: 5674 5674
Size: 6775
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2182 3394 1108
Size: 1074 1212 4489
Fragments arranged by size:
4489 1212 1074
PfoI T'CCnGG_A
Cuts at: 2058 2427 2793 2937 3582 3981 2058
Size: 369 366 144 645 399 4852
Fragments arranged by size:
4852 645 399 369 366 144
PmeI GTTT'AAAC
Cuts at: 4079 4079
Size: 6775
PmlI CAC'GTG
Cuts at: 3427 3427
Size: 6775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4844 4876 6357 6389 4844
Size: 32 1481 32 5230
Fragments arranged by size:
5230 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2743 3301 2743
Size: 558 6217
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6775
PsiI TTA'TAA
Cuts at: 4345 4345
Size: 6775
PspOMI G'GGCC_C
Cuts at: 3405 3738 3929 3405
Size: 333 191 6251
Fragments arranged by size:
6251 333 191
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3102 3134 3102
Size: 32 6743
PstI C_TGCA'G
Cuts at: 830 2421 3195 4127 830
Size: 1591 774 932 3478
Fragments arranged by size:
3478 1591 932 774
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6775
PvuII CAG'CTG
Cuts at: 2068 2614 2068
Size: 546 6229
SacI G_AGCT'C
Cuts at: 3369 3369
Size: 6775
SacII CC_GC'GG
Cuts at: 2812 2812
Size: 6775
SalI G'TCGA_C
Cuts at: 1936 4117 1936
Size: 2181 4594
SanDI GG'GwC_CC
Cuts at: 2743 2743
Size: 6775
SapI GCTCTTCn'nnn_
Cuts at: 3813 3813
Size: 6775
SbfI CC_TGCA'GG
Cuts at: 4127 4127
Size: 6775
ScaI AGT'ACT
Cuts at: 2603 5023 6735 2603
Size: 2420 1712 2643
Fragments arranged by size:
2643 2420 1712
SfcI C'TryA_G
Cuts at: 826 1045 2128 2417 3191 4123 5258 5939 6130
Size: 219 1083 289 774 932 1135 681 191
Cuts at: 6130 826
Size: 1471
Fragments arranged by size:
1471 1135 1083 932 774 681 289 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3404 3404
Size: 6775
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6775
SmaI CCC'GGG
Cuts at: 1872 3737 4102 1872
Size: 1865 365 4545
Fragments arranged by size:
4545 1865 365
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2705 2737 4879 5780 6042 820
Size: 197 926 762 32 2142 901 262 1553
Fragments arranged by size:
2142 1553 926 901 762 262 197 32
SnaBI TAC'GTA
Cuts at: 493 4566 493
Size: 4073 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6775
SphI G_CATG'C
Cuts at: 1132 2804 4133 1132
Size: 1672 1329 3774
Fragments arranged by size:
3774 1672 1329
SspI AAT'ATT
Cuts at: 5 52 4699 5
Size: 47 4647 2081
Fragments arranged by size:
4647 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6775
StyI C'CwwG_G
Cuts at: 513 1065 2358 2481 2533 3037 4214 513
Size: 552 1293 123 52 504 1177 3074
Fragments arranged by size:
3074 1293 1177 552 504 123 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2334 4074 4802 4819 4972 5157 5602
Size: 1255 754 1740 728 17 153 185 445
Cuts at: 5602 325
Size: 1498
Fragments arranged by size:
1740 1498 1255 754 728 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 3666 5021
Size: 274 80 33 51 1443 624 1065 1355
Cuts at: 5021 6733 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1355 1065 624 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2459 2491 3467 3499 6735 6767 2459
Size: 32 976 32 3236 32 2467
Fragments arranged by size:
3236 2467 976 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6775
XbaI T'CTAG_A
Cuts at: 4111 4111
Size: 6775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 3649 2183
Size: 1466 5309
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6775
XmaI C'CCGG_G
Cuts at: 1870 3735 4100 1870
Size: 1865 365 4545
Fragments arranged by size:
4545 1865 365
XmnI GAAnn'nnTTC
Cuts at: 4904 4904
Size: 6775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4583 276
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI
PacI PspXI RsrII SexAI SfoI SrfI SwaI