Restriction map for
pFN21AE9696
(Circular) (Six-base) MAPSORT of: pFN21AE9696.seq Check: 6586 from: 1 to: 10693
pFN21AE9696 10693 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2662 3188 6442 2662
Size: 526 3254 6913
Fragments arranged by size:
6913 3254 526
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8503 278
Size: 53 83 186 936 6967 2468
Fragments arranged by size:
6967 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6136 6926 8036 1937
Size: 4199 790 1110 4594
Fragments arranged by size:
4594 4199 1110 790
Acc65I G'GTAC_C
Cuts at: 6891 8014 6891
Size: 1123 9570
AclI AA'CG_TT
Cuts at: 1563 8820 9193 1563
Size: 7257 373 3063
Fragments arranged by size:
7257 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2482 3527 3680 5228 5712 6147 7347 7664
Size: 850 1045 153 1548 484 435 1200 317
Cuts at: 7664 7780 8756 10140 10408 1632
Size: 116 976 1384 268 1917
Fragments arranged by size:
1917 1548 1384 1200 1045 976 850 484
435 317 268 153 116
AfeI AGC'GCT
Cuts at: 6572 6572
Size: 10693
AflII C'TTAA_G
Cuts at: 820 1017 5066 820
Size: 197 4049 6447
Fragments arranged by size:
6447 4049 197
AflIII A'CryG_T
Cuts at: 1203 3408 4203 9592 10569 1203
Size: 2205 795 5389 977 1327
Fragments arranged by size:
5389 2205 1327 977 795
AgeI A'CCGG_T
Cuts at: 5538 9524 5538
Size: 3986 6707
AhdI GACnn_n'nnGTC
Cuts at: 6070 9422 6070
Size: 3352 7341
AleI CACnn'nnGTG
Cuts at: 1166 5579 5816 1166
Size: 4413 237 6043
Fragments arranged by size:
6043 4413 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10693
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 4385 4417 4652 4684 2438
Size: 32 1915 32 235 32 8447
Fragments arranged by size:
8447 1915 235 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2021 2624 2627 2660 2684 2954 4187 6310 6642
Size: 603 3 33 24 270 1233 2123 332
Cuts at: 6642 6782 7402 8436 10008 2021
Size: 140 620 1034 1572 2706
Fragments arranged by size:
2706 2123 1572 1233 1034 620 603 332
270 140 33 24 3
ApaI G_GGCC'C
Cuts at: 4249 5374 7819 4249
Size: 1125 2445 7123
Fragments arranged by size:
7123 2445 1125
ApaLI G'TGCA_C
Cuts at: 8750 9906 8750
Size: 1156 9537
ApoI r'AATT_y
Cuts at: 1493 2231 4421 4481 4999 5014 5681 8002 8228
Size: 738 2190 60 518 15 667 2321 226
Cuts at: 8228 8380 10489 1493
Size: 152 2109 1697
Fragments arranged by size:
2321 2190 2109 1697 738 667 518 226
152 60 15
AseI AT'TA_AT
Cuts at: 160 1032 9247 160
Size: 872 8215 1606
Fragments arranged by size:
8215 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10693
AvaI C'yCGr_G
Cuts at: 1870 1943 2829 2926 5400 6195 6429 7056 7189
Size: 73 886 97 2474 795 234 627 133
Cuts at: 7189 7962 8018 1870
Size: 773 56 4545
Fragments arranged by size:
4545 2474 886 795 773 627 234 133
97 73 56
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6875 6908 6875
Size: 33 10660
BamHI G'GATC_C
Cuts at: 8023 8390 8023
Size: 367 10326
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2872 4193 4414 5327 6853
Size: 325 217 627 1085 1321 221 913 1526
Cuts at: 6853 6862 6891 7832 8014 9469 618
Size: 9 29 941 182 1455 1842
Fragments arranged by size:
1842 1526 1455 1321 1085 941 913 627
325 221 217 182 29 9
BanII G_rGCy'C
Cuts at: 1391 2828 3232 3396 3762 4249 4758 4938 5374
Size: 1437 404 164 366 487 509 180 436
Cuts at: 5374 5503 5636 7819 7962 8012 1391
Size: 129 133 2183 143 50 4072
Fragments arranged by size:
4072 2183 1437 509 487 436 404 366
180 164 143 133 129 50
BbeI G_GCGC'C
Cuts at: 7836 7836
Size: 10693
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2351 4068 4303 5886 5991 6063 6114
Size: 971 452 1717 235 1583 105 72 51
Cuts at: 6114 7225 928
Size: 1111 4396
Fragments arranged by size:
4396 1717 1583 1111 971 452 235 105
72 51
BbvCI CC'TCA_GC
Cuts at: 2057 2955 5957 7703 2057
Size: 898 3002 1746 5047
Fragments arranged by size:
5047 3002 1746 898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3477 3511 8884 8918 1903
Size: 34 1540 34 5373 34 3678
Fragments arranged by size:
5373 3678 1540 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8585 9795 8585
Size: 1210 9483
BclI T'GATC_A
Cuts at: 1548 3352 4584 4594 1548
Size: 1804 1232 10 7647
Fragments arranged by size:
7647 1804 1232 10
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 9304 136
Size: 107 122 71 631 605 337 7295 1525
Fragments arranged by size:
7295 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 7206 10688 7206
Size: 3482 7211
BlpI GC'TnA_GC
Cuts at: 2667 4955 7607 8110 2667
Size: 2288 2652 503 5250
Fragments arranged by size:
5250 2652 2288 503
Bme1580I G_kGCm'C
Cuts at: 1790 4249 4256 4369 4417 5155 5332 5374 7727
Size: 2459 7 113 48 738 177 42 2353
Cuts at: 7727 7819 8754 9910 1790
Size: 92 935 1156 2573
Fragments arranged by size:
2573 2459 2353 1156 935 738 177 113
92 48 42 7
BmgBI CAC'GTC
Cuts at: 1318 5818 7627 1318
Size: 4500 1809 4384
Fragments arranged by size:
4500 4384 1809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4119 4399 5124
Size: 416 53 83 399 24 2702 280 725
Cuts at: 5124 5953 8424 9382 442
Size: 829 2471 958 1753
Fragments arranged by size:
2702 2471 1753 958 829 725 416 399
280 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10693
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3270 3270
Size: 10693
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 2456 3216 4054 4209 4769 4835 5171
Size: 819 398 760 838 155 560 66 336
Cuts at: 5171 6562 6648 6842 6888 7370 7412 7616 7875
Size: 1391 86 194 46 482 42 204 259
Cuts at: 7875 9353 1239
Size: 1478 2579
Fragments arranged by size:
2579 1478 1391 838 819 760 560 482
398 336 259 204 194 155 86 66
46 42
Bpu10I CC'TnA_GC
Cuts at: 2057 2955 3216 5957 6051 7703 2057
Size: 898 261 2741 94 1652 5047
Fragments arranged by size:
5047 2741 1652 898 261 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4640 4880 5288 6725 8818 9683 9981 4640
Size: 240 408 1437 2093 865 298 5352
Fragments arranged by size:
5352 2093 1437 865 408 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9356 882
Size: 617 7857 2219
Fragments arranged by size:
7857 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 8484 493
Size: 711 2774 4506 2702
Fragments arranged by size:
4506 2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 2564 3588 10687 2564
Size: 1024 7099 2570
Fragments arranged by size:
7099 2570 1024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6929 7833 8500 8882
Size: 53 83 186 936 5396 904 667 382
Cuts at: 8882 9517 275
Size: 635 1451
Fragments arranged by size:
5396 1451 936 904 667 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2294 2714 5538 5787 6156 9126 9524 9798 9945
Size: 420 2824 249 369 2970 398 274 147
Cuts at: 9945 10356 2294
Size: 411 2631
Fragments arranged by size:
2970 2824 2631 420 411 398 369 274
249 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 3383 3413 3764
Size: 30 165 30 1035 30 915 30 351
Cuts at: 3764 3794 3836 3866 4385 4415 6652 6682 6941
Size: 30 42 30 519 30 2237 30 259
Cuts at: 6941 6971 1178
Size: 30 4900
Fragments arranged by size:
4900 2237 1035 915 519 351 259 165
42 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2477 3110 3113 3329 3356 3359 3409
Size: 1054 237 633 3 216 27 3 50
Cuts at: 3409 3860 4766 4832 5747 5936 6110 6113 6565
Size: 451 906 66 915 189 174 3 452
Cuts at: 6565 6577 6962 7427 7613 1186
Size: 12 385 465 186 4266
Fragments arranged by size:
4266 1054 915 906 633 465 452 451
385 237 216 189 186 174 66 50
27 12 3 3 3
BseYI C'CCAG_C
Cuts at: 4071 4620 4870 5405 5629 6220 6270 6554 6791
Size: 549 250 535 224 591 50 284 237
Cuts at: 6791 6796 7241 9896 10392 4071
Size: 5 445 2655 496 4372
Fragments arranged by size:
4372 2655 591 549 535 496 445 284
250 237 224 50 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3539 4184 5840 6518 6628 7559 1163
Size: 573 1803 645 1656 678 110 931 4297
Fragments arranged by size:
4297 1803 1656 931 678 645 573 110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5353 8143 8904 9932 1540
Size: 275 186 3352 2790 761 1028 2301
Fragments arranged by size:
3352 2790 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3396 4758 4938 5232 6513 8754 8839 9910 3396
Size: 1362 180 294 1281 2241 85 1071 4179
Fragments arranged by size:
4179 2241 1362 1281 1071 294 180 85
BsmI GAATG_Cn'
Cuts at: 8204 8297 8204
Size: 93 10600
BsmBI CGTCTCn'nnnn_
Cuts at: 3110 6609 3110
Size: 3499 7194
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2828 3232 3396 3762 4249 4256 4369
Size: 399 1038 404 164 366 487 7 113
Cuts at: 4369 4417 4758 4938 5155 5232 5332 5374 5503
Size: 48 341 180 217 77 100 42 129
Cuts at: 5503 5636 6513 7727 7819 7962 8012 8754 8839
Size: 133 877 1214 92 143 50 742 85
Cuts at: 8839 9910 1391
Size: 1071 2174
Fragments arranged by size:
2174 1214 1071 1038 877 742 487 404
399 366 341 217 180 164 143 133
129 113 100 92 85 77 50 48
42 7
BspEI T'CCGG_A
Cuts at: 2714 6156 10356 2714
Size: 3442 4200 3051
Fragments arranged by size:
4200 3442 3051
BspHI T'CATG_A
Cuts at: 2508 5055 8582 10312 2508
Size: 2547 3527 1730 2889
Fragments arranged by size:
3527 2889 2547 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2662 3188 6442 6664 8048 844
Size: 1818 526 3254 222 1384 3489
Fragments arranged by size:
3489 3254 1818 1384 526 222
BsrBI CCG'CTC
Cuts at: 1394 1823 4396 6817 8580 1394
Size: 429 2573 2421 1763 3507
Fragments arranged by size:
3507 2573 2421 1763 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 9188 9362 65
Size: 1183 7940 174 1396
Fragments arranged by size:
7940 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3000 3042 5538 7456 7558 7707 7744
Size: 110 1190 42 2496 1918 102 149 37
Cuts at: 7744 9337 9524 1700
Size: 1593 187 2869
Fragments arranged by size:
2869 2496 1918 1593 1190 187 149 110
102 42 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10693
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10693
BssSI C'ACGA_G
Cuts at: 3186 5019 8753 9765 3186
Size: 1833 3734 1012 4114
Fragments arranged by size:
4114 3734 1833 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4867 794
Size: 4073 6620
BstBI TT'CG_AA
Cuts at: 9497 9497
Size: 10693
BstEII G'GTnAC_C
Cuts at: 1184 7031 1184
Size: 5847 4846
BstXI CCAn_nnnn'nTGG
Cuts at: 3155 3155
Size: 10693
BstYI r'GATC_y
Cuts at: 1972 2541 3525 5062 7206 8023 8390 8775 8792
Size: 569 984 1537 2144 817 367 385 17
Cuts at: 8792 10244 10330 10342 10688 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2144 1977 1537 1452 984 817 569 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2995 5333 6323 6618 2995
Size: 2338 990 295 7070
Fragments arranged by size:
7070 2338 990 295
BtgI C'CryG_G
Cuts at: 513 1065 4169 5100 6895 7067 7255 7470 513
Size: 552 3104 931 1795 172 188 215 3736
Fragments arranged by size:
3736 3104 1795 931 552 215 188 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5755 7651 487
Size: 683 56 4529 1896 3529
Fragments arranged by size:
4529 3529 1896 683 56
BtsI GCAGTG_nn'
Cuts at: 2287 2986 3090 3567 3673 5430 5749 6176 6185
Size: 699 104 477 106 1757 319 427 9
Cuts at: 6185 8209 9003 9023 2287
Size: 2024 794 20 3957
Fragments arranged by size:
3957 2024 1757 794 699 477 427 319
106 104 20 9
ClaI AT'CG_AT
Cuts at: 1554 5310 5349 1554
Size: 3756 39 6898
Fragments arranged by size:
6898 3756 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3416 3451 529
Size: 35 2852 35 7771
Fragments arranged by size:
7771 2852 35 35
DraI TTT'AAA
Cuts at: 7321 7997 7321
Size: 676 10017
DraIII CAC_nnn'GTG
Cuts at: 3913 5024 5227 6306 7158 3913
Size: 1111 203 1079 852 7448
Fragments arranged by size:
7448 1111 1079 852 203
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 9700 809
Size: 507 4238 4146 1802
Fragments arranged by size:
4238 4146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 3044 3087
Size: 54 1425 50 275 18 864 350 43
Cuts at: 3087 5427 5512 5583 7509 7560 7713 7821 8140
Size: 2340 85 71 1926 51 153 108 319
Cuts at: 8140 9029 10658 8
Size: 889 1629 43
Fragments arranged by size:
2340 1926 1629 1425 889 864 350 319
275 153 108 85 71 54 51 50
43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8140 1537
Size: 275 6328 4090
Fragments arranged by size:
6328 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 2767 3306 3621 3794 4529 4763 5559
Size: 791 623 539 315 173 735 234 796
Cuts at: 5559 5688 8623 1353
Size: 129 2935 3423
Fragments arranged by size:
3423 2935 796 791 735 623 539 315
234 173 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2371 2830 7637 9280 9654 9800
Size: 1064 138 782 459 4807 1643 374 146
Cuts at: 9800 387
Size: 1280
Fragments arranged by size:
4807 1643 1280 1064 782 459 374 146
138
EcoICRI GAG'CTC
Cuts at: 3394 4756 4936 3394
Size: 1362 180 9151
Fragments arranged by size:
9151 1362 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2456 2482 3216 3527 3680 4054
Size: 393 426 398 26 734 311 153 374
Cuts at: 4054 4209 4769 4835 5171 5228 5712 6147 6562
Size: 155 560 66 336 57 484 435 415
Cuts at: 6562 6648 6842 6888 7347 7370 7412 7616 7664
Size: 86 194 46 459 23 42 204 48
Cuts at: 7664 7780 7875 8756 9353 10140 10408 1239
Size: 116 95 881 597 787 268 1524
Fragments arranged by size:
1524 881 787 734 597 560 484 459
435 426 415 398 393 374 336 311
268 204 194 155 153 116 95 86
66 57 48 46 42 26 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3618 5336 7394 7448 7532 7577 3618
Size: 1718 2058 54 84 45 6734
Fragments arranged by size:
6734 2058 1718 84 54 45
EcoO109I rG'GnC_Cy
Cuts at: 2162 2431 2514 2939 3067 4349 5370 5410 6319
Size: 269 83 425 128 1282 1021 40 909
Cuts at: 6319 6424 7052 7760 7815 7816 7860 2162
Size: 105 628 708 55 1 44 4995
Fragments arranged by size:
4995 1282 1021 909 708 628 425 269
128 105 83 55 44 40 1
EcoRI G'AATT_C
Cuts at: 4481 5014 8002 8380 4481
Size: 533 2988 378 6794
Fragments arranged by size:
6794 2988 533 378
EcoRV GAT'ATC
Cuts at: 2752 2752
Size: 10693
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7360 7360
Size: 10693
FseI GG_CCGG'CC
Cuts at: 3004 3046 3004
Size: 42 10651
FspI TGC'GCA
Cuts at: 9199 9199
Size: 10693
HaeII r_GCGC'y
Cuts at: 1678 6561 6574 7403 7803 7836 9840 1678
Size: 4883 13 829 400 33 2004 2531
Fragments arranged by size:
4883 2531 2004 829 400 33 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2660 2692 3764 3796 5217
Size: 32 135 32 1088 32 1072 32 1421
Cuts at: 5217 5249 5895 5927 6056 6088 7055 7087 9334
Size: 32 646 32 129 32 967 32 2247
Cuts at: 9334 9366 9408 9440 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2247 1421 1088 1072 967 646 135
129 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6034 6459 6927 8037 8283 669
Size: 988 281 4096 425 468 1110 246 3079
Fragments arranged by size:
4096 3079 1110 988 468 425 281 246
HindIII A'AGCT_T
Cuts at: 748 2852 4521 748
Size: 2104 1669 6920
Fragments arranged by size:
6920 2104 1669
HpaI GTT'AAC
Cuts at: 6034 8283 6034
Size: 2249 8444
KasI G'GCGC_C
Cuts at: 7832 7832
Size: 10693
KpnI G_GTAC'C
Cuts at: 6895 8018 6895
Size: 1123 9570
MfeI C'AATT_G
Cuts at: 8292 8292
Size: 10693
MluI A'CGCG_T
Cuts at: 3408 10569 3408
Size: 7161 3532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2285 4194 5763 6939 9807 9991 1305
Size: 980 1909 1569 1176 2868 184 2007
Fragments arranged by size:
2868 2007 1909 1569 1176 980 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5429 5514 5585 7715 10
Size: 54 1425 343 3597 85 71 2130 2988
Fragments arranged by size:
3597 2988 2130 1425 343 85 71 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5579 5816 5967 6215 6381 6516 7260
Size: 648 4413 237 151 248 166 135 744
Cuts at: 7260 8651 9010 9169 518
Size: 1391 359 159 2042
Fragments arranged by size:
4413 2042 1391 744 648 359 248 237
166 159 151 135
MspA1I CmG'CkG
Cuts at: 1606 2056 2624 2642 2660 2681 3700 3739 4620
Size: 450 568 18 18 21 1019 39 881
Cuts at: 4620 6241 7420 7594 8109 8786 9934 10179 1606
Size: 1621 1179 174 515 677 1148 245 2120
Fragments arranged by size:
2120 1621 1179 1148 1019 881 677 568
515 450 245 174 39 21 18 18
NaeI GCC'GGC
Cuts at: 3002 3044 7458 7560 7709 3002
Size: 42 4414 102 149 5986
Fragments arranged by size:
5986 4414 149 102 42
NarI GG'CG_CC
Cuts at: 7833 7833
Size: 10693
NcoI C'CATG_G
Cuts at: 513 1065 7255 7470 513
Size: 552 6190 215 3736
Fragments arranged by size:
6190 3736 552 215
NdeI CA'TA_TG
Cuts at: 387 3964 387
Size: 3577 7116
NgoMIV G'CCGG_C
Cuts at: 3000 3042 7456 7558 7707 3000
Size: 42 4414 102 149 5986
Fragments arranged by size:
5986 4414 149 102 42
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10693
NotI GC'GGCC_GC
Cuts at: 8140 8140
Size: 10693
NspI r_CATG'y
Cuts at: 1132 1229 2453 3498 4207 4885 4921 8051 9596
Size: 97 1224 1045 709 678 36 3130 1545
Cuts at: 9596 1132
Size: 2229
Fragments arranged by size:
3130 2229 1545 1224 1045 709 678 97
36
PasI CC'CwG_GG
Cuts at: 1348 4249 1348
Size: 2901 7792
PciI A'CATG_T
Cuts at: 4203 9592 4203
Size: 5389 5304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3913 4620 6306 6809 1108
Size: 2805 707 1686 503 4992
Fragments arranged by size:
4992 2805 1686 707 503
PfoI T'CCnGG_A
Cuts at: 2074 4812 7389 2074
Size: 2738 2577 5378
Fragments arranged by size:
5378 2738 2577
PmeI GTTT'AAAC
Cuts at: 7997 7997
Size: 10693
PmlI CAC'GTG
Cuts at: 3978 3978
Size: 10693
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2470 3623 3655 4385 4417 8762 8794 10275
Size: 32 1153 32 730 32 4345 32 1481
Cuts at: 10275 10307 2438
Size: 32 2824
Fragments arranged by size:
4345 2824 1481 1153 730 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2162 2939 3067 4349 6319 6424 7760 7860 2162
Size: 777 128 1282 1970 105 1336 100 4995
Fragments arranged by size:
4995 1970 1336 1282 777 128 105 100
PshAI GACnn'nnGTC
Cuts at: 1613 2937 1613
Size: 1324 9369
PsiI TTA'TAA
Cuts at: 8263 8263
Size: 10693
PspOMI G'GGCC_C
Cuts at: 4245 5370 7815 4245
Size: 1125 2445 7123
Fragments arranged by size:
7123 2445 1125
PspXI vC'TCGA_Gb
Cuts at: 2926 2926
Size: 10693
PstI C_TGCA'G
Cuts at: 830 3085 7858 8045 830
Size: 2255 4773 187 3478
Fragments arranged by size:
4773 3478 2255 187
PvuI CG_AT'CG
Cuts at: 2001 5353 2001
Size: 3352 7341
PvuII CAG'CTG
Cuts at: 2056 2624 2660 2681 3700 7420 7594 2056
Size: 568 36 21 1019 3720 174 5155
Fragments arranged by size:
5155 3720 1019 568 174 36 21
RsrII CG'GwC_CG
Cuts at: 2297 2297
Size: 10693
SacI G_AGCT'C
Cuts at: 3396 4758 4938 3396
Size: 1362 180 9151
Fragments arranged by size:
9151 1362 180
SalI G'TCGA_C
Cuts at: 1936 6925 8035 1936
Size: 4989 1110 4594
Fragments arranged by size:
4989 4594 1110
SanDI GG'GwC_CC
Cuts at: 6424 7760 6424
Size: 1336 9357
SapI GCTCTTCn'nnn_
Cuts at: 2767 3794 4763 5559 5688 2767
Size: 1027 969 796 129 7772
Fragments arranged by size:
7772 1027 969 796 129
SbfI CC_TGCA'GG
Cuts at: 7858 8045 7858
Size: 187 10506
ScaI AGT'ACT
Cuts at: 3365 3854 6080 8941 10653 3365
Size: 489 2226 2861 1712 3405
Fragments arranged by size:
3405 2861 2226 1712 489
SexAI A'CCwGG_T
Cuts at: 5034 5610 5034
Size: 576 10117
SfcI C'TryA_G
Cuts at: 826 1045 2661 3081 3555 4106 7854 8041 9176
Size: 219 1616 420 474 551 3748 187 1135
Cuts at: 9176 9857 10048 826
Size: 681 191 1471
Fragments arranged by size:
3748 1616 1471 1135 681 551 474 420
219 191 187
SfoI GGC'GCC
Cuts at: 7834 7834
Size: 10693
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10693
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10693
SmaI CCC'GGG
Cuts at: 1872 8020 1872
Size: 6148 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2926 4655 4895 5066 5267 6704
Size: 197 926 983 1729 240 171 201 1437
Cuts at: 6704 7056 8797 9698 9960 820
Size: 352 1741 901 262 1553
Fragments arranged by size:
1741 1729 1553 1437 983 926 901 352
262 240 201 197 171
SnaBI TAC'GTA
Cuts at: 493 8484 493
Size: 7991 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10693
SphI G_CATG'C
Cuts at: 1132 8051 1132
Size: 6919 3774
SspI AAT'ATT
Cuts at: 5 52 8617 5
Size: 47 8565 2081
Fragments arranged by size:
8565 2081 47
StuI AGG'CCT
Cuts at: 1517 5009 5489 6353 1517
Size: 3492 480 864 5857
Fragments arranged by size:
5857 3492 864 480
StyI C'CwwG_G
Cuts at: 513 1065 2569 3288 3687 5637 6827 7255 7470
Size: 552 1504 719 399 1950 1190 428 215
Cuts at: 7470 7533 8132 513
Size: 63 599 3074
Fragments arranged by size:
3074 1950 1504 1190 719 599 552 428
399 215 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 2779 2891 4432 8720 8737 8890
Size: 1255 549 650 112 1541 4288 17 153
Cuts at: 8890 9075 9520 325
Size: 185 445 1498
Fragments arranged by size:
4288 1541 1498 1255 650 549 445 185
153 112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3363 3852 6078
Size: 274 80 33 51 1443 1386 489 2226
Cuts at: 6078 8939 10651 96
Size: 2861 1712 138
Fragments arranged by size:
2861 2226 1712 1443 1386 489 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3383 3415 4126 4158 4333 4365 5211 5243 10653
Size: 32 711 32 175 32 846 32 5410
Cuts at: 10653 10685 3383
Size: 32 3391
Fragments arranged by size:
5410 3391 846 711 175 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2301 5441 6441 1316
Size: 985 3140 1000 5568
Fragments arranged by size:
5568 3140 1000 985
XbaI T'CTAG_A
Cuts at: 6841 8029 6841
Size: 1188 9505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2082 3240 4426 6307 7540 2082
Size: 1158 1186 1881 1233 5235
Fragments arranged by size:
5235 1881 1233 1186 1158
XhoI C'TCGA_G
Cuts at: 1943 2926 7056 1943
Size: 983 4130 5580
Fragments arranged by size:
5580 4130 983
XmaI C'CCGG_G
Cuts at: 1870 8018 1870
Size: 6148 4545
XmnI GAAnn'nnTTC
Cuts at: 3165 4481 6248 8822 3165
Size: 1316 1767 2574 5036
Fragments arranged by size:
5036 2574 1767 1316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8501 276
Size: 53 83 186 936 6967 2468
Fragments arranged by size:
6967 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BstZ17I FspAI NruI NsiI
PacI PsrI SacII SfiI SrfI SwaI