Restriction map for
pFN21AE9695
(Circular) (Six-base) MAPSORT of: pFN21AE9695.seq Check: 2428 from: 1 to: 6670
pFN21AE9695 6670 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3349 3957 3349
Size: 608 6062
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3575 4480 278
Size: 53 83 186 936 2039 905 2468
Fragments arranged by size:
2468 2039 936 905 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2772 4013 1937
Size: 835 1241 4594
Fragments arranged by size:
4594 1241 835
Acc65I G'GTAC_C
Cuts at: 3991 3991
Size: 6670
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3419 3770 4733 6117 6385 1632
Size: 1787 351 963 1384 268 1917
Fragments arranged by size:
1917 1787 1384 963 351 268
AflII C'TTAA_G
Cuts at: 820 1017 3872 820
Size: 197 2855 3618
Fragments arranged by size:
3618 2855 197
AflIII A'CryG_T
Cuts at: 1203 2070 2912 5569 6546 1203
Size: 867 842 2657 977 1327
Fragments arranged by size:
2657 1327 977 867 842
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 2015 1166
Size: 849 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6670
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 6638
AlwNI CAG_nnn'CTG
Cuts at: 2751 2980 4413 5985 2751
Size: 229 1433 1572 3436
Fragments arranged by size:
3436 1572 1433 229
ApaI G_GGCC'C
Cuts at: 2703 2703
Size: 6670
ApaLI G'TGCA_C
Cuts at: 3670 4727 5883 3670
Size: 1057 1156 4457
Fragments arranged by size:
4457 1156 1057
ApoI r'AATT_y
Cuts at: 1493 2292 3979 4205 4357 6466 1493
Size: 799 1687 226 152 2109 1697
Fragments arranged by size:
2109 1697 1687 799 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2940 3995 1870
Size: 73 282 715 1055 4545
Fragments arranged by size:
4545 1055 715 282 73
BamHI G'GATC_C
Cuts at: 4000 4367 4000
Size: 367 6303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 3991 5446 618
Size: 325 217 627 1505 699 1455 1842
Fragments arranged by size:
1842 1505 1455 699 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2235 2261 2703 3210 3390 3989 1391
Size: 844 26 442 507 180 599 4072
Fragments arranged by size:
4072 844 599 507 442 180 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2416 928
Size: 971 517 5182
Fragments arranged by size:
5182 971 517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4861 4895 1903
Size: 34 2924 34 3678
Fragments arranged by size:
3678 2924 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4562 5772 4562
Size: 1210 5460
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2184 2837 3394
Size: 107 122 71 631 605 512 653 557
Cuts at: 3394 3915 3924 5281 136
Size: 521 9 1357 1525
Fragments arranged by size:
1525 1357 653 631 605 557 521 512
122 107 71 9
BglII A'GATC_T
Cuts at: 3156 6665 3156
Size: 3509 3161
BlpI GC'TnA_GC
Cuts at: 3354 4087 3354
Size: 733 5937
Bme1580I G_kGCm'C
Cuts at: 1790 2442 2703 3674 4731 5887 1790
Size: 652 261 971 1057 1156 2573
Fragments arranged by size:
2573 1156 1057 971 652 261
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6670
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2192 2827 3317
Size: 416 53 83 399 24 775 635 490
Cuts at: 3317 4401 5359 442
Size: 1084 958 1753
Fragments arranged by size:
1753 1084 958 775 635 490 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2678 3323 5330 1239
Size: 1439 645 2007 2579
Fragments arranged by size:
2579 2007 1439 645
Bpu10I CC'TnA_GC
Cuts at: 2787 3911 2787
Size: 1124 5546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4795 5660 5958 4795
Size: 865 298 5507
Fragments arranged by size:
5507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5333 882
Size: 617 3834 2219
Fragments arranged by size:
3834 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2899 4461 493
Size: 711 1695 1562 2702
Fragments arranged by size:
2702 1695 1562 711
BsaBI GATnn'nnATC
Cuts at: 6664 6664
Size: 6670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2492 3188 3572 4477
Size: 53 83 186 936 959 696 384 905
Cuts at: 4477 4859 5494 275
Size: 382 635 1451
Fragments arranged by size:
1451 959 936 905 696 635 384 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2463 5103 5501 5775 5922 6333 2463
Size: 2640 398 274 147 411 2800
Fragments arranged by size:
2800 2640 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2876 2906 1178
Size: 30 165 30 1473 30 4942
Fragments arranged by size:
4942 1473 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2881 3848 1186
Size: 1057 638 967 4008
Fragments arranged by size:
4008 1057 967 638
BseYI C'CCAG_C
Cuts at: 2086 2778 2990 3023 3086 3263 3435 5873 6369
Size: 692 212 33 63 177 172 2438 496
Cuts at: 6369 2086
Size: 2387
Fragments arranged by size:
2438 2387 692 496 212 177 172 63
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2972 3201 3302 3327 3724 3929 1163
Size: 573 1236 229 101 25 397 205 3904
Fragments arranged by size:
3904 1236 573 397 229 205 101 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2499 3919 4120 4881 5909 1540
Size: 275 186 498 1420 201 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 498 275 201 186
BsiHKAI G_wGCw'C
Cuts at: 2235 3510 3674 4731 4816 5887 2235
Size: 1275 164 1057 85 1071 3018
Fragments arranged by size:
3018 1275 1071 1057 164 85
BsiWI C'GTAC_G
Cuts at: 2349 2349
Size: 6670
BsmI GAATG_Cn'
Cuts at: 4181 4274 4181
Size: 93 6577
BsmBI CGTCTCn'nnnn_
Cuts at: 2893 2893
Size: 6670
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2261 2442 2703 3210 3390 3510
Size: 399 445 26 181 261 507 180 120
Cuts at: 3510 3674 3989 4731 4816 5887 1391
Size: 164 315 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 507 445 399 315 261
181 180 164 120 85 26
BspEI T'CCGG_A
Cuts at: 6333 6333
Size: 6670
BspHI T'CATG_A
Cuts at: 2172 4559 6289 2172
Size: 2387 1730 2553
Fragments arranged by size:
2553 2387 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 3349 3957 4025 844
Size: 2197 308 608 68 3489
Fragments arranged by size:
3489 2197 608 308 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2791 3019 3915 4557 1394
Size: 429 968 228 896 642 3507
Fragments arranged by size:
3507 968 896 642 429 228
BsrDI GCAATG_nn'
Cuts at: 65 1248 2854 5165 5339 65
Size: 1183 1606 2311 174 1396
Fragments arranged by size:
2311 1606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2243 2537 2961 3852 5314 5501 1700
Size: 110 433 294 424 891 1462 187 2869
Fragments arranged by size:
2869 1462 891 433 424 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 2742 96
Size: 2646 4024
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 4730 5742 4730
Size: 1012 5658
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3033 794
Size: 2239 4431
BstBI TT'CG_AA
Cuts at: 5474 5474
Size: 6670
BstEII G'GTnAC_C
Cuts at: 1184 2020 1184
Size: 836 5834
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 3502 2017
Size: 1485 5185
BstYI r'GATC_y
Cuts at: 1972 2527 2905 3129 3156 3429 3474 4000 4367
Size: 555 378 224 27 273 45 526 367
Cuts at: 4367 4752 4769 6221 6307 6319 6665 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 555 526 385 378 367 346
273 224 86 45 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3273 3555 3922 513
Size: 552 939 1269 282 367 3261
Fragments arranged by size:
3261 1269 939 552 367 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5931
Fragments arranged by size:
5931 683 56
BtsI GCAGTG_nn'
Cuts at: 2532 2762 2978 4186 4980 5000 2532
Size: 230 216 1208 794 20 4202
Fragments arranged by size:
4202 1208 794 230 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DraIII CAC_nnn'GTG
Cuts at: 2515 2768 3498 2515
Size: 253 730 5687
Fragments arranged by size:
5687 730 253
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2135 5677 809
Size: 507 819 3542 1802
Fragments arranged by size:
3542 1802 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2241 2496
Size: 54 1425 50 275 18 177 234 255
Cuts at: 2496 2829 2838 3591 3916 3957 4117 5006 6635
Size: 333 9 753 325 41 160 889 1629
Cuts at: 6635 8
Size: 43
Fragments arranged by size:
1629 1425 889 753 333 325 275 255
234 177 160 54 50 43 41 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 2496 3916 4117 1537
Size: 275 684 1420 201 4090
Fragments arranged by size:
4090 1420 684 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2723 4600 1353
Size: 1370 1877 3423
Fragments arranged by size:
3423 1877 1370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2201 5257 5631 5777 387
Size: 1064 138 612 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 612 374 146 138
EcoICRI GAG'CTC
Cuts at: 2233 2233
Size: 6670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2678 3323 3419 3770 4733 5330 6117
Size: 393 1046 645 96 351 963 597 787
Cuts at: 6117 6385 1239
Size: 268 1524
Fragments arranged by size:
1524 1046 963 787 645 597 393 351
268 96
EcoO109I rG'GnC_Cy
Cuts at: 2275 2573 2653 2944 3307 2275
Size: 298 80 291 363 5638
Fragments arranged by size:
5638 363 298 291 80
EcoRI G'AATT_C
Cuts at: 3979 4357 3979
Size: 378 6292
EcoRV GAT'ATC
Cuts at: 3823 3823
Size: 6670
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 2997 3079 5817 1678
Size: 1319 82 2738 2531
Fragments arranged by size:
2738 2531 1319 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3101 3133 3452 3484 5311
Size: 32 135 32 1529 32 319 32 1827
Cuts at: 5311 5343 5385 5417 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1827 1529 319 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2140 2581 2815 3637 4014 4260
Size: 988 281 202 441 234 822 377 246
Cuts at: 4260 669
Size: 3079
Fragments arranged by size:
3079 988 822 441 377 281 246 234
202
HindIII A'AGCT_T
Cuts at: 748 3698 748
Size: 2950 3720
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KpnI G_GTAC'C
Cuts at: 3995 3995
Size: 6670
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 2912 6546 2912
Size: 3634 3036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3164 3432 5784 5968 1305
Size: 1859 268 2352 184 2007
Fragments arranged by size:
2352 2007 1859 268 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2840 3593 3959 10
Size: 54 1425 343 177 831 753 366 2721
Fragments arranged by size:
2721 1425 831 753 366 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 2306 3278 3500 3788 4628 4987
Size: 648 849 291 972 222 288 840 359
Cuts at: 4987 5146 518
Size: 159 2042
Fragments arranged by size:
2042 972 849 840 648 359 291 288
222 159
MspA1I CmG'CkG
Cuts at: 1606 2748 3587 3766 3885 4086 4763 5911 6156
Size: 1142 839 179 119 201 677 1148 245
Cuts at: 6156 1606
Size: 2120
Fragments arranged by size:
2120 1148 1142 839 677 245 201 179
119
NaeI GCC'GGC
Cuts at: 2245 2539 3854 2245
Size: 294 1315 5061
Fragments arranged by size:
5061 1315 294
NcoI C'CATG_G
Cuts at: 513 1065 2004 3273 3555 3922 513
Size: 552 939 1269 282 367 3261
Fragments arranged by size:
3261 1269 939 552 367 282
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NgoMIV G'CCGG_C
Cuts at: 2243 2537 3852 2243
Size: 294 1315 5061
Fragments arranged by size:
5061 1315 294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 3916 4117 3916
Size: 201 6469
NspI r_CATG'y
Cuts at: 1132 1229 2063 3793 4028 5573 1132
Size: 97 834 1730 235 1545 2229
Fragments arranged by size:
2229 1730 1545 834 235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6670
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6670
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2653 2944 3307 2275
Size: 378 291 363 5638
Fragments arranged by size:
5638 378 363 291
PshAI GACnn'nnGTC
Cuts at: 1613 2915 1613
Size: 1302 5368
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PspOMI G'GGCC_C
Cuts at: 2699 2699
Size: 6670
PstI C_TGCA'G
Cuts at: 830 2333 3038 3708 3765 4022 830
Size: 1503 705 670 57 257 3478
Fragments arranged by size:
3478 1503 705 670 257 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
SacI G_AGCT'C
Cuts at: 2235 2235
Size: 6670
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SanDI GG'GwC_CC
Cuts at: 2944 2944
Size: 6670
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 4918 6630 4918
Size: 1712 4958
SfcI C'TryA_G
Cuts at: 826 1045 2115 2329 3034 3704 3761 4018 5153
Size: 219 1070 214 705 670 57 257 1135
Cuts at: 5153 5834 6025 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1070 705 681 670 257 219
214 191 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 2837 3915 2837
Size: 1078 5592
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 2942 3997 1872
Size: 1070 1055 4545
Fragments arranged by size:
4545 1070 1055
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3872 4774 5675 5937 820
Size: 197 926 1929 902 901 262 1553
Fragments arranged by size:
1929 1553 926 902 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 2063 4028 1132
Size: 931 1965 3774
Fragments arranged by size:
3774 1965 931
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 3404 3909 1517
Size: 1887 505 4278
Fragments arranged by size:
4278 1887 505
StyI C'CwwG_G
Cuts at: 513 1065 2004 3273 3348 3555 3922 4109 513
Size: 552 939 1269 75 207 367 187 3074
Fragments arranged by size:
3074 1269 939 552 367 207 187 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3361 3803 4697 4714 4867 5052 5497
Size: 1255 1781 442 894 17 153 185 445
Cuts at: 5497 325
Size: 1498
Fragments arranged by size:
1781 1498 1255 894 445 442 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 4916 6628
Size: 274 80 33 51 1443 765 2174 1712
Cuts at: 6628 96
Size: 138
Fragments arranged by size:
2174 1712 1443 765 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2404 2436 6630 6662 2404
Size: 32 4194 32 2412
Fragments arranged by size:
4194 2412 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6670
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3271 3271
Size: 6670
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6670
XmaI C'CCGG_G
Cuts at: 1870 2940 3995 1870
Size: 1070 1055 4545
Fragments arranged by size:
4545 1070 1055
XmnI GAAnn'nnTTC
Cuts at: 4799 4799
Size: 6670
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3573 4478 276
Size: 53 83 186 936 2039 905 2468
Fragments arranged by size:
2468 2039 936 905 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacII SapI SexAI SfoI SrfI SwaI