Restriction map for
pFN21AE9691
(Circular) (Six-base) MAPSORT of: pFN21AE9691.seq Check: 6235 from: 1 to: 6520
pFN21AE9691 6520 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2619 3072 3863 1937
Size: 682 453 791 4594
Fragments arranged by size:
4594 791 682 453
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2073 2414 2469 2696 3051 3101 3345 3602
Size: 441 341 55 227 355 50 244 257
Cuts at: 3602 4583 5967 6235 1632
Size: 981 1384 268 1917
Fragments arranged by size:
1917 1384 981 441 355 341 268 257
244 227 55 50
AflII C'TTAA_G
Cuts at: 820 1017 3637 820
Size: 197 2620 3703
Fragments arranged by size:
3703 2620 197
AflIII A'CryG_T
Cuts at: 1203 2464 5419 6396 1203
Size: 1261 2955 977 1327
Fragments arranged by size:
2955 1327 1261 977
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 5249 5249
Size: 6520
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6520
AlwNI CAG_nnn'CTG
Cuts at: 4263 5835 4263
Size: 1572 4948
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 2939 3029 3037 3829 4055 4207 6316 1493
Size: 1446 90 8 792 226 152 2109 1697
Fragments arranged by size:
2109 1697 1446 792 226 152 90 8
AseI AT'TA_AT
Cuts at: 160 1032 5074 160
Size: 872 4042 1606
Fragments arranged by size:
4042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 3422 3845 1870
Size: 73 1479 423 4545
Fragments arranged by size:
4545 1479 423 73
BamHI G'GATC_C
Cuts at: 2749 3850 4217 2749
Size: 1101 367 5052
Fragments arranged by size:
5052 1101 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3841 5296 618
Size: 325 217 627 2054 1455 1842
Fragments arranged by size:
2054 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3214 3839 1391
Size: 1823 625 4072
Fragments arranged by size:
4072 1823 625
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2475 2540 3774 928
Size: 971 576 65 1234 3674
Fragments arranged by size:
3674 1234 971 576 65
BbvCI CC'TCA_GC
Cuts at: 3630 3630
Size: 6520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6520
BfrBI ATG'CAT
Cuts at: 3401 3401
Size: 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5131 136
Size: 107 122 71 631 605 337 3122 1525
Fragments arranged by size:
3122 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2707 6515 2707
Size: 3808 2712
BlpI GC'TnA_GC
Cuts at: 3937 3937
Size: 6520
Bme1580I G_kGCm'C
Cuts at: 1790 3812 4581 5737 1790
Size: 2022 769 1156 2573
Fragments arranged by size:
2573 2022 1156 769
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3799 4251 5209
Size: 416 53 83 399 24 2382 452 958
Cuts at: 5209 442
Size: 1753
Fragments arranged by size:
2382 1753 958 452 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2720 3044 3239 3780 5180 1239
Size: 1058 423 324 195 541 1400 2579
Fragments arranged by size:
2579 1400 1058 541 423 324 195
Bpu10I CC'TnA_GC
Cuts at: 3630 3630
Size: 6520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 4645 5510 5808 2192
Size: 2453 865 298 2904
Fragments arranged by size:
2904 2453 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2934 3794 5183 882
Size: 617 1435 860 1389 2219
Fragments arranged by size:
2219 1435 1389 860 617
BsaAI yAC'GTr
Cuts at: 493 1204 4311 493
Size: 711 3107 2702
Fragments arranged by size:
3107 2702 711
BsaBI GATnn'nnATC
Cuts at: 6514 6514
Size: 6520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4327 4709 5344 275
Size: 53 83 186 936 2794 382 635 1451
Fragments arranged by size:
2794 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4953 5351 5625 5772 6183 4953
Size: 398 274 147 411 5290
Fragments arranged by size:
5290 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3452 3482 1178
Size: 30 165 30 2049 30 4216
Fragments arranged by size:
4216 2049 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2657 3473 3540 1186
Size: 1228 243 816 67 4166
Fragments arranged by size:
4166 1228 816 243 67
BseYI C'CCAG_C
Cuts at: 5723 6219 5723
Size: 496 6024
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2058 2489 1163
Size: 573 322 431 5194
Fragments arranged by size:
5194 573 431 322
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3970 4731 5759 1540
Size: 275 186 1969 761 1028 2301
Fragments arranged by size:
2301 1969 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3372 3421 4581 4666 5737 3372
Size: 49 1160 85 1071 4155
Fragments arranged by size:
4155 1160 1071 85 49
BsmI GAATG_Cn'
Cuts at: 4031 4124 4031
Size: 93 6427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3214 3372 3421 3812 3839 4581 4666
Size: 399 1424 158 49 391 27 742 85
Cuts at: 4666 5737 1391
Size: 1071 2174
Fragments arranged by size:
2174 1424 1071 742 399 391 158 85
49 27
BspEI T'CCGG_A
Cuts at: 6183 6183
Size: 6520
BspHI T'CATG_A
Cuts at: 4409 6139 4409
Size: 1730 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3875 844
Size: 3031 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4407 1394
Size: 429 2584 3507
Fragments arranged by size:
3507 2584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2811 5015 5189 65
Size: 1183 1563 2204 174 1396
Fragments arranged by size:
2204 1563 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2622 5164 5351 1700
Size: 110 812 2542 187 2869
Fragments arranged by size:
2869 2542 812 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 2691 4580 5592 2691
Size: 1889 1012 3619
Fragments arranged by size:
3619 1889 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6520
BstBI TT'CG_AA
Cuts at: 5324 5324
Size: 6520
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6520
BstYI r'GATC_y
Cuts at: 1972 2707 2749 3850 4217 4602 4619 6071 6157
Size: 735 42 1101 367 385 17 1452 86
Cuts at: 6157 6169 6515 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1101 735 385 367 346 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2308 3567 513
Size: 552 939 304 1259 3466
Fragments arranged by size:
3466 1259 939 552 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5781
Fragments arranged by size:
5781 683 56
BtsI GCAGTG_nn'
Cuts at: 4036 4830 4850 4036
Size: 794 20 5706
Fragments arranged by size:
5706 794 20
ClaI AT'CG_AT
Cuts at: 1554 3439 1554
Size: 1885 4635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5527 809
Size: 507 4211 1802
Fragments arranged by size:
4211 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3967 4856 6485
Size: 54 1425 50 275 18 2137 889 1629
Cuts at: 6485 8
Size: 43
Fragments arranged by size:
2137 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3967 1537
Size: 275 2155 4090
Fragments arranged by size:
4090 2155 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2505 3321 4450 1353
Size: 699 453 816 1129 3423
Fragments arranged by size:
3423 1129 816 699 453
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5107 5481 5627 387
Size: 1064 138 3518 374 146 1280
Fragments arranged by size:
3518 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2297 2414 2469 2696 2720 3044
Size: 393 441 224 117 55 227 24 324
Cuts at: 3044 3051 3101 3239 3345 3602 3780 4583 5180
Size: 7 50 138 106 257 178 803 597
Cuts at: 5180 5967 6235 1239
Size: 787 268 1524
Fragments arranged by size:
1524 803 787 597 441 393 324 268
257 227 224 178 138 117 106 55
50 24 7
EcoRI G'AATT_C
Cuts at: 3829 4207 3829
Size: 378 6142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2393 2499 2393
Size: 106 6414
FspI TGC'GCA
Cuts at: 5026 5026
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 5667 1678
Size: 3989 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2482 2514 3452
Size: 32 135 32 453 32 425 32 938
Cuts at: 3452 3484 5161 5193 5235 5267 1373
Size: 32 1677 32 42 32 2626
Fragments arranged by size:
2626 1677 938 453 425 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3073 3409 3864 4110 669
Size: 988 281 1135 336 455 246 3079
Fragments arranged by size:
3079 1135 988 455 336 281 246
HindIII A'AGCT_T
Cuts at: 748 3692 748
Size: 2944 3576
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 2123 4119 2123
Size: 1996 4524
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2703 2952 5634 5818 1305
Size: 1398 249 2682 184 2007
Fragments arranged by size:
2682 2007 1398 249 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4698
Fragments arranged by size:
4698 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4478 4837 4996 518
Size: 648 3312 359 159 2042
Fragments arranged by size:
3312 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2392 3208 3580 3623 3936 4613 5761 6006
Size: 786 816 372 43 313 677 1148 245
Cuts at: 6006 1606
Size: 2120
Fragments arranged by size:
2120 1148 816 786 677 372 313 245
43
NcoI C'CATG_G
Cuts at: 513 1065 2004 2308 513
Size: 552 939 304 4725
Fragments arranged by size:
4725 939 552 304
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NsiI A_TGCA'T
Cuts at: 3403 3403
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 2482 3405 3878 5423 1132
Size: 97 1253 923 473 1545 2229
Fragments arranged by size:
2229 1545 1253 923 473 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6520
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6520
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4589 4621 6102 6134 4589
Size: 32 1481 32 4975
Fragments arranged by size:
4975 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6520
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PstI C_TGCA'G
Cuts at: 830 3872 830
Size: 3042 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 2392 3580 2392
Size: 1188 5332
SalI G'TCGA_C
Cuts at: 1936 3071 3862 1936
Size: 1135 791 4594
Fragments arranged by size:
4594 1135 791
SapI GCTCTTCn'nnn_
Cuts at: 2052 2505 2052
Size: 453 6067
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SfcI C'TryA_G
Cuts at: 826 1045 2915 3868 5003 5684 5875 826
Size: 219 1870 953 1135 681 191 1471
Fragments arranged by size:
1870 1471 1135 953 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 3847 1872
Size: 1975 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 3637 4624 5525 5787 820
Size: 197 926 264 1430 987 901 262 1553
Fragments arranged by size:
1553 1430 987 926 901 264 262 197
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 2092 152
Size: 1940 4580
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 3635 1517
Size: 2118 4402
StyI C'CwwG_G
Cuts at: 513 1065 2004 2308 2745 3959 513
Size: 552 939 304 437 1214 3074
Fragments arranged by size:
3074 1214 939 552 437 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4547 4564 4717 4902 5347 325
Size: 1255 2967 17 153 185 445 1498
Fragments arranged by size:
2967 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3346 4766 6478
Size: 274 80 33 51 1443 1369 1420 1712
Cuts at: 6478 96
Size: 138
Fragments arranged by size:
1712 1443 1420 1369 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2304 2336 6480 6512 2304
Size: 32 4144 32 2312
Fragments arranged by size:
4144 2312 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3466 1316
Size: 2150 4370
XbaI T'CTAG_A
Cuts at: 3214 3856 3214
Size: 642 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 3845 1870
Size: 1975 4545
XmnI GAAnn'nnTTC
Cuts at: 4649 4649
Size: 6520
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI