Restriction map for
pFN21AE9682
(Circular) (Six-base) MAPSORT of: pFN21AE9682.seq Check: 135 from: 1 to: 5674
pFN21AE9682 5674 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 2793 3737 5121 5389 1632
Size: 914 247 944 1384 268 1917
Fragments arranged by size:
1917 1384 944 914 268 247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 4573 5550 1203
Size: 3370 977 1327
Fragments arranged by size:
3370 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 3417 4989 3417
Size: 1572 4102
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2346 2418 2983 3209 3361 5470 1493
Size: 853 72 565 226 152 2109 1697
Fragments arranged by size:
2109 1697 853 565 226 152 72
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2999 1870
Size: 73 76 980 4545
Fragments arranged by size:
4545 980 76 73
BamHI G'GATC_C
Cuts at: 2584 3004 3371 2584
Size: 420 367 4887
Fragments arranged by size:
4887 420 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 4450 618
Size: 325 217 627 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2117 2382 2993 1391
Size: 726 265 611 4072
Fragments arranged by size:
4072 726 611 265
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2166 2676 2788 928
Size: 971 267 510 112 3814
Fragments arranged by size:
3814 971 510 267 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3865 3899 1903
Size: 34 50 34 1844 34 3678
Fragments arranged by size:
3678 1844 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2653 2768 5669 2653
Size: 115 2901 2658
Fragments arranged by size:
2901 2658 115
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 3735 4891 1790
Size: 1945 1156 2573
Fragments arranged by size:
2573 1945 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4334 1239
Size: 3095 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3799 4664 4962 3799
Size: 865 298 4511
Fragments arranged by size:
4511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 2128 4337 882
Size: 617 531 98 2209 2219
Fragments arranged by size:
2219 2209 617 531 98
BsaAI yAC'GTr
Cuts at: 493 1204 2813 3465 493
Size: 711 1609 652 2702
Fragments arranged by size:
2702 1609 711 652
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 1186
Size: 1318 4356
BseYI C'CCAG_C
Cuts at: 4877 5373 4877
Size: 496 5178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2741 1163
Size: 573 1005 4096
Fragments arranged by size:
4096 1005 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2117 2382 3735 3820 4891 2117
Size: 265 1353 85 1071 2900
Fragments arranged by size:
2900 1353 1071 265 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2382 2993 3735 3820 4891 1391
Size: 399 327 265 611 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 399 327 265 85
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 844
Size: 2185 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2700 4169 4343 65
Size: 1183 1452 1469 174 1396
Fragments arranged by size:
1469 1452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4318 4505 1700
Size: 110 2508 187 2869
Fragments arranged by size:
2869 2508 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2584 2653 2768 3004 3371 3756 3773 5225
Size: 612 69 115 236 367 385 17 1452
Cuts at: 5225 5311 5323 5669 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 612 385 367 346 236 115
86 69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DraIII CAC_nnn'GTG
Cuts at: 2719 2719
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639
Size: 54 1425 50 275 18 1291 889 1629
Cuts at: 5639 8
Size: 43
Fragments arranged by size:
1629 1425 1291 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 2769 3604 1353
Size: 1152 264 835 3423
Fragments arranged by size:
3423 1152 835 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2115 2380 2115
Size: 265 5409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2546 2793 3737 4334 5121 5389 1239
Size: 393 914 247 944 597 787 268 1524
Fragments arranged by size:
1524 944 914 787 597 393 268 247
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2255 2255
Size: 5674
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2102 2134 2183
Size: 32 135 32 457 32 41 32 49
Cuts at: 2183 2215 2522 2549 2554 2569 2581 2601 4315
Size: 32 307 27 5 15 12 20 1714
Cuts at: 4315 4347 4389 4421 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1714 457 307 135 49 42 41
32 32 32 32 32 32 32 27
20 15 12 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 3264 669
Size: 988 281 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2424 2924 748
Size: 1676 500 3498
Fragments arranged by size:
3498 1676 500
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4788 4972 1305
Size: 3483 184 2007
Fragments arranged by size:
3483 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 3632 3991 4150 518
Size: 648 883 1583 359 159 2042
Fragments arranged by size:
2042 1583 883 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2320 3090 3767 4915 5160 1606
Size: 714 770 677 1148 245 2120
Fragments arranged by size:
2120 1148 770 714 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5122
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 3032 4577 1132
Size: 97 1803 1545 2229
Fragments arranged by size:
2229 1803 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 2244 3244 2244
Size: 1000 4674
PstI C_TGCA'G
Cuts at: 830 3026 830
Size: 2196 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SacI G_AGCT'C
Cuts at: 2117 2382 2117
Size: 265 5409
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2371 2942 3022 4157 4838 5029 826
Size: 219 1326 571 80 1135 681 191 1471
Fragments arranged by size:
1471 1326 1135 681 571 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3778 4679 4941 820
Size: 197 926 1835 901 262 1553
Fragments arranged by size:
1835 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 2957 3598 5
Size: 47 2905 641 2081
Fragments arranged by size:
2905 2081 641 47
StuI AGG'CCT
Cuts at: 1517 2905 1517
Size: 1388 4286
StyI C'CwwG_G
Cuts at: 513 1065 3113 513
Size: 552 2048 3074
Fragments arranged by size:
3074 2048 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2110 3701 3718 3871 4056 4501 325
Size: 1255 530 1591 17 153 185 445 1498
Fragments arranged by size:
1591 1498 1255 530 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3920 5632 96
Size: 274 80 33 51 1443 1943 1712 138
Fragments arranged by size:
1943 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 2956 3803 2956
Size: 847 4827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI