Restriction map for
pFN21AE9679
(Circular) (Six-base) MAPSORT of: pFN21AE9679.seq Check: 4810 from: 1 to: 13351
pFN21AE9679 13351 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11161 278
Size: 53 83 186 936 9625 2468
Fragments arranged by size:
9625 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2860 4080 4329 5913 10694 1937
Size: 923 1220 249 1584 4781 4594
Fragments arranged by size:
4781 4594 1584 1220 923 249
Acc65I G'GTAC_C
Cuts at: 6988 10672 6988
Size: 3684 9667
AclI AA'CG_TT
Cuts at: 1563 4806 11478 11851 1563
Size: 3243 6672 373 3063
Fragments arranged by size:
6672 3243 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2700 2849 3218 3660 3822 3987 4852 5198
Size: 1068 149 369 442 162 165 865 346
Cuts at: 5198 5846 6429 6705 8202 8505 8835 9959 11414
Size: 648 583 276 1497 303 330 1124 1455
Cuts at: 11414 12798 13066 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1497 1455 1384 1124 1068 865 648
583 442 369 346 330 303 276 268
165 162 149
AflII C'TTAA_G
Cuts at: 820 1017 5140 9497 820
Size: 197 4123 4357 4674
Fragments arranged by size:
4674 4357 4123 197
AflIII A'CryG_T
Cuts at: 1203 2278 3755 5819 7352 9637 12250 13227 1203
Size: 1075 1477 2064 1533 2285 2613 977 1327
Fragments arranged by size:
2613 2285 2064 1533 1477 1327 1075 977
AgeI A'CCGG_T
Cuts at: 12182 12182
Size: 13351
AhdI GACnn_n'nnGTC
Cuts at: 12080 12080
Size: 13351
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 13351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 9066 10038 1184
Size: 7882 972 4497
Fragments arranged by size:
7882 4497 972
AlwNI CAG_nnn'CTG
Cuts at: 2099 3095 6734 7160 8417 8735 9896 9899 10012
Size: 996 3639 426 1257 318 1161 3 113
Cuts at: 10012 11094 12666 2099
Size: 1082 1572 2784
Fragments arranged by size:
3639 2784 1572 1257 1161 1082 996 426
318 113 3
ApaLI G'TGCA_C
Cuts at: 3074 11408 12564 3074
Size: 8334 1156 3861
Fragments arranged by size:
8334 3861 1156
ApoI r'AATT_y
Cuts at: 1493 2122 2135 2237 2575 2596 2755 3001 3169
Size: 629 13 102 338 21 159 246 168
Cuts at: 3169 4003 4086 4148 4781 5174 5483 6122 7404
Size: 834 83 62 633 393 309 639 1282
Cuts at: 7404 7607 9396 9727 10660 10886 11038 13147 1493
Size: 203 1789 331 933 226 152 2109 1697
Fragments arranged by size:
2109 1789 1697 1282 933 834 639 633
629 393 338 331 309 246 226 203
168 159 152 102 83 62 21 13
AseI AT'TA_AT
Cuts at: 160 1032 9429 11905 160
Size: 872 8397 2476 1606
Fragments arranged by size:
8397 2476 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13351
AvaI C'yCGr_G
Cuts at: 1870 1943 3221 5778 6824 9508 10676 1870
Size: 73 1278 2557 1046 2684 1168 4545
Fragments arranged by size:
4545 2684 2557 1278 1168 1046 73
AvrII C'CTAG_G
Cuts at: 8099 8099
Size: 13351
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5376 5409 5376
Size: 33 13318
BamHI G'GATC_C
Cuts at: 4227 7726 10681 11048 4227
Size: 3499 2955 367 6530
Fragments arranged by size:
6530 3499 2955 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5081 5275 6988 7432 8966
Size: 325 217 627 3294 194 1713 444 1534
Cuts at: 8966 9206 10672 12127 618
Size: 240 1466 1455 1842
Fragments arranged by size:
3294 1842 1713 1534 1466 1455 627 444
325 240 217 194
BanII G_rGCy'C
Cuts at: 1391 3656 5424 6607 8946 10670 1391
Size: 2265 1768 1183 2339 1724 4072
Fragments arranged by size:
4072 2339 2265 1768 1724 1183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3236 3782 3828 5111 7694 8169 8190
Size: 971 1337 546 46 1283 2583 475 21
Cuts at: 8190 9084 9571 10185 10524 10653 928
Size: 894 487 614 339 129 3626
Fragments arranged by size:
3626 2583 1337 1283 971 894 614 546
487 475 339 129 46 21
BbvCI CC'TCA_GC
Cuts at: 5463 5463
Size: 13351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 11542 11576 1903
Size: 34 9605 34 3678
Fragments arranged by size:
9605 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4360 11243 12453 4360
Size: 6883 1210 5258
Fragments arranged by size:
6883 5258 1210
BclI T'GATC_A
Cuts at: 1548 3130 8799 8989 1548
Size: 1582 5669 190 5910
Fragments arranged by size:
5910 5669 1582 190
BfrBI ATG'CAT
Cuts at: 2081 2455 2660 2081
Size: 374 205 12772
Fragments arranged by size:
12772 374 205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 11962 136
Size: 107 122 71 631 605 10290 1525
Fragments arranged by size:
10290 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4453 6064 7273 13346 4453
Size: 1611 1209 6073 4458
Fragments arranged by size:
6073 4458 1611 1209
BlpI GC'TnA_GC
Cuts at: 10768 10768
Size: 13351
Bme1580I G_kGCm'C
Cuts at: 1790 3078 11412 12568 1790
Size: 1288 8334 1156 2573
Fragments arranged by size:
8334 2573 1288 1156
BmgBI CAC'GTC
Cuts at: 1318 6194 1318
Size: 4876 8475
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4780 5984 8940
Size: 416 53 83 399 24 3363 1204 2956
Cuts at: 8940 10414 11082 12040 442
Size: 1474 668 958 1753
Fragments arranged by size:
3363 2956 1753 1474 1204 958 668 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4146 5666 8292 8940 9648 10080 10371 12011
Size: 2907 1520 2626 648 708 432 291 1640
Cuts at: 12011 1239
Size: 2579
Fragments arranged by size:
2907 2626 2579 1640 1520 708 648 432
291
Bpu10I CC'TnA_GC
Cuts at: 5423 5463 5423
Size: 40 13311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 9375 11476 12341 12639 9375
Size: 2101 865 298 10087
Fragments arranged by size:
10087 2101 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 6442 8268 9134 12014 882
Size: 617 549 4394 1826 866 2880 2219
Fragments arranged by size:
4394 2880 2219 1826 866 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 4791 11142 493
Size: 711 3587 6351 2702
Fragments arranged by size:
6351 3587 2702 711
BsaBI GATnn'nnATC
Cuts at: 2040 4452 5520 5844 6141 8389 13345 2040
Size: 2412 1068 324 297 2248 4956 2046
Fragments arranged by size:
4956 2412 2248 2046 1068 324 297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7363 11158 11540 12175
Size: 53 83 186 936 5830 3795 382 635
Cuts at: 12175 275
Size: 1451
Fragments arranged by size:
5830 3795 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6237 6444 11784 12182 12456 12603 13014 6237
Size: 207 5340 398 274 147 411 6574
Fragments arranged by size:
6574 5340 411 398 274 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6439 6469 7263 7293 7681
Size: 30 165 30 5036 30 794 30 388
Cuts at: 7681 7711 8088 8118 8159 8189 9750 9780 1178
Size: 30 377 30 41 30 1561 30 4749
Fragments arranged by size:
5036 4749 1561 794 388 377 165 41
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4911 5480 6651 8073 8147 8180 8548 8551
Size: 3725 569 1171 1422 74 33 368 3
Cuts at: 8551 8554 8557 9331 10077 1186
Size: 3 3 774 746 4460
Fragments arranged by size:
4460 3725 1422 1171 774 746 569 368
74 33 3 3 3
BseYI C'CCAG_C
Cuts at: 2269 7108 7225 8350 10025 12554 13050 2269
Size: 4839 117 1125 1675 2529 496 2570
Fragments arranged by size:
4839 2570 2529 1675 1125 496 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2151 1163
Size: 573 415 12363
Fragments arranged by size:
12363 573 415
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 10801 11562 12590 1540
Size: 275 186 8800 761 1028 2301
Fragments arranged by size:
8800 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3078 4276 4409 5424 5610 6607 8681 9088 9507
Size: 1198 133 1015 186 997 2074 407 419
Cuts at: 9507 11412 11497 12568 3078
Size: 1905 85 1071 3861
Fragments arranged by size:
3861 2074 1905 1198 1071 1015 997 419
407 186 133 85
BsiWI C'GTAC_G
Cuts at: 4787 4787
Size: 13351
BsmI GAATG_Cn'
Cuts at: 3025 3394 6491 6906 7670 9552 10044 10862 10955
Size: 369 3097 415 764 1882 492 818 93
Cuts at: 10955 3025
Size: 5421
Fragments arranged by size:
5421 3097 1882 818 764 492 415 369
93
BsmBI CGTCTCn'nnnn_
Cuts at: 5236 7370 9847 5236
Size: 2134 2477 8740
Fragments arranged by size:
8740 2477 2134
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3078 3656 4276 4409 5424 5610 6607
Size: 399 1288 578 620 133 1015 186 997
Cuts at: 6607 8681 8946 9088 9507 10670 11412 11497 12568
Size: 2074 265 142 419 1163 742 85 1071
Cuts at: 12568 1391
Size: 2174
Fragments arranged by size:
2174 2074 1288 1163 1071 1015 997 742
620 578 419 399 265 186 142 133
85
BspEI T'CCGG_A
Cuts at: 6444 13014 6444
Size: 6570 6781
BspHI T'CATG_A
Cuts at: 3304 5049 7900 9556 11240 12970 3304
Size: 1745 2851 1656 1684 1730 3685
Fragments arranged by size:
3685 2851 1745 1730 1684 1656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6516 7228 10706 844
Size: 5672 712 3478 3489
Fragments arranged by size:
5672 3489 3478 712
BsrBI CCG'CTC
Cuts at: 1394 1823 11238 1394
Size: 429 9415 3507
Fragments arranged by size:
9415 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4409 5538 11846 12020 65
Size: 1183 3161 1129 6308 174 1396
Fragments arranged by size:
6308 3161 1396 1183 1129 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8969 10046 11995 12182 1700
Size: 110 7159 1077 1949 187 2869
Fragments arranged by size:
7159 2869 1949 1077 187 110
BsrGI T'GTAC_A
Cuts at: 96 2086 10438 96
Size: 1990 8352 3009
Fragments arranged by size:
8352 3009 1990
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13351
BssSI C'ACGA_G
Cuts at: 3642 6699 11411 12423 3642
Size: 3057 4712 1012 4570
Fragments arranged by size:
4712 4570 3057 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2882 7160 8075 9896 9899 10012 794
Size: 2088 4278 915 1821 3 113 4133
Fragments arranged by size:
4278 4133 2088 1821 915 113 3
BstBI TT'CG_AA
Cuts at: 6073 6811 12155 6073
Size: 738 5344 7269
Fragments arranged by size:
7269 5344 738
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 13351
BstXI CCAn_nnnn'nTGG
Cuts at: 2339 5546 5888 7884 8285 2339
Size: 3207 342 1996 401 7405
Fragments arranged by size:
7405 3207 1996 401 342
BstYI r'GATC_y
Cuts at: 1972 2011 2417 3091 4227 4453 5182 6064 6667
Size: 39 406 674 1136 226 729 882 603
Cuts at: 6667 7204 7273 7726 9961 10598 10681 11048 11433
Size: 537 69 453 2235 637 83 367 385
Cuts at: 11433 11450 12902 12988 13000 13346 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2235 1977 1452 1136 882 729 674 637
603 537 453 406 385 367 346 226
86 83 69 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 3039 3039
Size: 13351
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 12799
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3492 4993 487
Size: 683 56 2266 1501 8845
Fragments arranged by size:
8845 2266 1501 683 56
BtsI GCAGTG_nn'
Cuts at: 7371 10010 10867 11661 11681 7371
Size: 2639 857 794 20 9041
Fragments arranged by size:
9041 2639 857 794 20
ClaI AT'CG_AT
Cuts at: 1554 6141 1554
Size: 4587 8764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 13316
DraI TTT'AAA
Cuts at: 7069 7237 10655 7069
Size: 168 3418 9765
Fragments arranged by size:
9765 3418 168
DraIII CAC_nnn'GTG
Cuts at: 5068 7939 5068
Size: 2871 10480
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8731 12358 809
Size: 507 7415 3627 1802
Fragments arranged by size:
7415 3627 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6553 8780 10798
Size: 54 1425 50 275 18 4723 2227 2018
Cuts at: 10798 11687 13316 8
Size: 889 1629 43
Fragments arranged by size:
4723 2227 2018 1629 1425 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10798 1537
Size: 275 8986 4090
Fragments arranged by size:
8986 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3159 3467 3966 4197 4464 6007 6070 6126
Size: 1806 308 499 231 267 1543 63 56
Cuts at: 6126 7519 8470 8545 8557 8575 9978 11281 1353
Size: 1393 951 75 12 18 1403 1303 3423
Fragments arranged by size:
3423 1806 1543 1403 1393 1303 951 499
308 267 231 75 63 56 18 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 11938 12312 12458 387
Size: 1064 138 10349 374 146 1280
Fragments arranged by size:
10349 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5422 6605 5422
Size: 1183 12168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2700 2849 3218 3660 3822 3987 4146
Size: 393 1068 149 369 442 162 165 159
Cuts at: 4146 4852 5198 5666 5846 6429 6705 8202 8292
Size: 706 346 468 180 583 276 1497 90
Cuts at: 8292 8505 8835 8940 9648 9959 10080 10371 11414
Size: 213 330 105 708 311 121 291 1043
Cuts at: 11414 12011 12798 13066 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1497 1068 1043 787 708 706 597
583 468 442 393 369 346 330 311
291 276 268 213 180 165 162 159
149 121 105 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2946 3233 3236 7584 8162 10059 2946
Size: 287 3 4348 578 1897 6238
Fragments arranged by size:
6238 4348 1897 578 287 3
EcoO109I rG'GnC_Cy
Cuts at: 2096 3226 4730 5038 7816 2096
Size: 1130 1504 308 2778 7631
Fragments arranged by size:
7631 2778 1504 1130 308
EcoRI G'AATT_C
Cuts at: 2575 3001 4003 9396 10660 11038 2575
Size: 426 1002 5393 1264 378 4888
Fragments arranged by size:
5393 4888 1264 1002 426 378
EcoRV GAT'ATC
Cuts at: 6139 6139
Size: 13351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4226 4749 5166 5324 6086 6099 7490 8087 10296
Size: 523 417 158 762 13 1391 597 2209
Cuts at: 10296 4226
Size: 7281
Fragments arranged by size:
7281 2209 1391 762 597 523 417 158
13
FspI TGC'GCA
Cuts at: 11857 11857
Size: 13351
HaeII r_GCGC'y
Cuts at: 1678 12498 1678
Size: 10820 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3833 3865 3878 3910 4732
Size: 32 135 32 2261 32 13 32 822
Cuts at: 4732 4764 6101 6133 6530 6532 6562 6564 7247
Size: 32 1337 32 397 2 30 2 683
Cuts at: 7247 7279 7681 7713 7765 7797 8159 8191 8267
Size: 32 402 32 52 32 362 32 76
Cuts at: 8267 8299 8393 8411 8425 8443 10448 10480 10577
Size: 32 94 18 14 18 2005 32 97
Cuts at: 10577 10595 10609 10627 11992 12024 12066 12098 1373
Size: 18 14 18 1365 32 42 32 2626
Fragments arranged by size:
2626 2261 2005 1365 1337 822 683 402
397 362 135 97 94 76 52 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 18
18 18 18 14 14 13 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2635 3100 3267 6201 7301 8997
Size: 988 281 697 465 167 2934 1100 1696
Cuts at: 8997 9030 9283 10695 10941 669
Size: 33 253 1412 246 3079
Fragments arranged by size:
3079 2934 1696 1412 1100 988 697 465
281 253 246 167 33
HindIII A'AGCT_T
Cuts at: 748 2467 4488 7850 748
Size: 1719 2021 3362 6249
Fragments arranged by size:
6249 3362 2021 1719
HpaI GTT'AAC
Cuts at: 2635 10941 2635
Size: 8306 5045
KpnI G_GTAC'C
Cuts at: 6992 10676 6992
Size: 3684 9667
MfeI C'AATT_G
Cuts at: 4037 4074 4624 4968 6177 10950 4037
Size: 37 550 344 1209 4773 6438
Fragments arranged by size:
6438 4773 1209 550 344 37
MluI A'CGCG_T
Cuts at: 13227 13227
Size: 13351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2207 6704 7667 8790 9655 10433 12465 12649
Size: 902 4497 963 1123 865 778 2032 184
Cuts at: 12649 1305
Size: 2007
Fragments arranged by size:
4497 2032 2007 1123 963 902 865 778
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 8782 10
Size: 54 1425 343 6950 4579
Fragments arranged by size:
6950 4579 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3126 3258 3423 7077 7679 11309 11668
Size: 648 1960 132 165 3654 602 3630 359
Cuts at: 11668 11827 518
Size: 159 2042
Fragments arranged by size:
3654 3630 2042 1960 648 602 359 165
159 132
MspA1I CmG'CkG
Cuts at: 1606 2273 5507 5813 9896 10313 10319 10767 11444
Size: 667 3234 306 4083 417 6 448 677
Cuts at: 11444 12592 12837 1606
Size: 1148 245 2120
Fragments arranged by size:
4083 3234 2120 1148 677 667 448 417
306 245 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 12799
NdeI CA'TA_TG
Cuts at: 387 4417 5938 6662 387
Size: 4030 1521 724 7076
Fragments arranged by size:
7076 4030 1521 724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13351
NotI GC'GGCC_GC
Cuts at: 10798 10798
Size: 13351
NsiI A_TGCA'T
Cuts at: 2083 2457 2662 2083
Size: 374 205 12772
Fragments arranged by size:
12772 374 205
NspI r_CATG'y
Cuts at: 1132 1229 2282 3759 5823 9799 10709 12254 1132
Size: 97 1053 1477 2064 3976 910 1545 2229
Fragments arranged by size:
3976 2229 2064 1545 1477 1053 910 97
PasI CC'CwG_GG
Cuts at: 1348 2567 6741 1348
Size: 1219 4174 7958
Fragments arranged by size:
7958 4174 1219
PciI A'CATG_T
Cuts at: 2278 3755 5819 12250 2278
Size: 1477 2064 6431 3379
Fragments arranged by size:
6431 3379 2064 1477
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6270 8249 9071 9203 1108
Size: 5162 1979 822 132 5256
Fragments arranged by size:
5256 5162 1979 822 132
PmeI GTTT'AAAC
Cuts at: 10655 10655
Size: 13351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3466 3498 9795 9827 10225 10257 11420 11452 12933
Size: 32 6297 32 398 32 1163 32 1481
Cuts at: 12933 12965 3466
Size: 32 3852
Fragments arranged by size:
6297 3852 1481 1163 398 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 3226 4730 2096
Size: 1130 1504 10717
Fragments arranged by size:
10717 1504 1130
PshAI GACnn'nnGTC
Cuts at: 1613 6196 1613
Size: 4583 8768
PsiI TTA'TAA
Cuts at: 5453 10921 5453
Size: 5468 7883
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4017 4049 4017
Size: 32 13319
PstI C_TGCA'G
Cuts at: 830 2878 5812 10318 10703 830
Size: 2048 2934 4506 385 3478
Fragments arranged by size:
4506 3478 2934 2048 385
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13351
PvuII CAG'CTG
Cuts at: 2273 5507 5813 9896 10313 10319 2273
Size: 3234 306 4083 417 6 5305
Fragments arranged by size:
5305 4083 3234 417 306 6
SacI G_AGCT'C
Cuts at: 5424 6607 5424
Size: 1183 12168
SalI G'TCGA_C
Cuts at: 1936 10693 1936
Size: 8757 4594
SapI GCTCTTCn'nnn_
Cuts at: 3467 4197 3467
Size: 730 12621
SbfI CC_TGCA'GG
Cuts at: 10703 10703
Size: 13351
ScaI AGT'ACT
Cuts at: 4429 8632 11599 13311 4429
Size: 4203 2967 1712 4469
Fragments arranged by size:
4469 4203 2967 1712
SexAI A'CCwGG_T
Cuts at: 4018 4018
Size: 13351
SfcI C'TryA_G
Cuts at: 826 1045 2874 5724 5808 5914 6707 8005 8051
Size: 219 1829 2850 84 106 793 1298 46
Cuts at: 8051 8928 9237 10314 10476 10699 11834 12515 12706
Size: 877 309 1077 162 223 1135 681 191
Cuts at: 12706 826
Size: 1471
Fragments arranged by size:
2850 1829 1471 1298 1135 1077 877 793
681 309 223 219 191 162 106 84
46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13351
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13351
SmaI CCC'GGG
Cuts at: 1872 9510 10678 1872
Size: 7638 1168 4545
Fragments arranged by size:
7638 4545 1168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5140 9390 9497 11455 12356 12618
Size: 197 926 3197 4250 107 1958 901 262
Cuts at: 12618 820
Size: 1553
Fragments arranged by size:
4250 3197 1958 1553 926 901 262 197
107
SnaBI TAC'GTA
Cuts at: 493 11142 493
Size: 10649 2702
SpeI A'CTAG_T
Cuts at: 152 2107 152
Size: 1955 11396
SphI G_CATG'C
Cuts at: 1132 10709 1132
Size: 9577 3774
SspI AAT'ATT
Cuts at: 5 52 3021 3637 4534 5317 7661 7735 11275
Size: 47 2969 616 897 783 2344 74 3540
Cuts at: 11275 5
Size: 2081
Fragments arranged by size:
3540 2969 2344 2081 897 783 616 74
47
StuI AGG'CCT
Cuts at: 1517 4498 6723 9980 10392 1517
Size: 2981 2225 3257 412 4476
Fragments arranged by size:
4476 3257 2981 2225 412
StyI C'CwwG_G
Cuts at: 513 1065 6210 8099 9202 10790 513
Size: 552 5145 1889 1103 1588 3074
Fragments arranged by size:
5145 3074 1889 1588 1103 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 2655 5973 6510 11378 11395 11548
Size: 1255 537 538 3318 537 4868 17 153
Cuts at: 11548 11733 12178 325
Size: 185 445 1498
Fragments arranged by size:
4868 3318 1498 1255 538 537 537 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2086 2525 4427
Size: 274 80 33 51 1443 109 439 1902
Cuts at: 4427 4863 8630 10438 10607 11597 13309 96
Size: 436 3767 1808 169 990 1712 138
Fragments arranged by size:
3767 1902 1808 1712 1443 990 439 436
274 169 138 109 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6439 6471 7462 7494 13311 13343 6439
Size: 32 991 32 5817 32 6447
Fragments arranged by size:
6447 5817 991 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 13351
XbaI T'CTAG_A
Cuts at: 4097 8128 10687 4097
Size: 4031 2559 6761
Fragments arranged by size:
6761 4031 2559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5971 7717 8285 8905 5971
Size: 1746 568 620 10417
Fragments arranged by size:
10417 1746 620 568
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 13351
XmaI C'CCGG_G
Cuts at: 1870 9508 10676 1870
Size: 7638 1168 4545
Fragments arranged by size:
7638 4545 1168
XmnI GAAnn'nnTTC
Cuts at: 2929 3663 4710 4938 6807 9639 9807 11480 2929
Size: 734 1047 228 1869 2832 168 1673 4800
Fragments arranged by size:
4800 2832 1869 1673 1047 734 228 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11159 276
Size: 53 83 186 936 9625 2468
Fragments arranged by size:
9625 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BplI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI RsrII SacII SanDI SfiI
SfoI SrfI SwaI