Restriction map for
pFN21AE9671
(Circular) (Six-base) MAPSORT of: pFN21AE9671.seq Check: 5621 from: 1 to: 9553
pFN21AE9671 9553 bp
With 182 enzymes: SgfI *
May 24, 2012 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3673 6718 3673
Size: 3045 6508
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2323 7363 278
Size: 53 83 186 936 787 5040 2468
Fragments arranged by size:
5040 2468 936 787 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3876 6374 6896 1937
Size: 1939 2498 522 4594
Fragments arranged by size:
4594 2498 1939 522
Acc65I G'GTAC_C
Cuts at: 6874 6874
Size: 9553
AclI AA'CG_TT
Cuts at: 1563 7680 8053 1563
Size: 6117 373 3063
Fragments arranged by size:
6117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2798 3217 3424 5609 5674 5864 6556 7616
Size: 1166 419 207 2185 65 190 692 1060
Cuts at: 7616 9000 9268 1632
Size: 1384 268 1917
Fragments arranged by size:
2185 1917 1384 1166 1060 692 419 268
207 190 65
AfeI AGC'GCT
Cuts at: 5831 5831
Size: 9553
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9356
AflIII A'CryG_T
Cuts at: 1203 6000 8452 9429 1203
Size: 4797 2452 977 1327
Fragments arranged by size:
4797 2452 1327 977
AgeI A'CCGG_T
Cuts at: 8384 8384
Size: 9553
AhdI GACnn_n'nnGTC
Cuts at: 6115 8282 6115
Size: 2167 7386
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5600 6021 6758 1184
Size: 4416 421 737 3979
Fragments arranged by size:
4416 3979 737 421
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4655 4687 5804 5836 4655
Size: 32 1117 32 8372
Fragments arranged by size:
8372 1117 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2776 3082 3145 5635 5766 7296 8868 2776
Size: 306 63 2490 131 1530 1572 3461
Fragments arranged by size:
3461 2490 1572 1530 306 131 63
ApaI G_GGCC'C
Cuts at: 2528 6418 2528
Size: 3890 5663
ApaLI G'TGCA_C
Cuts at: 5884 7610 8766 5884
Size: 1726 1156 6671
Fragments arranged by size:
6671 1726 1156
ApoI r'AATT_y
Cuts at: 1493 2156 4028 4676 6862 7088 7240 9349 1493
Size: 663 1872 648 2186 226 152 2109 1697
Fragments arranged by size:
2186 2109 1872 1697 663 648 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8107 160
Size: 872 7075 1606
Fragments arranged by size:
7075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9553
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 3482 4418 4556 4745 5210 5261
Size: 73 85 1454 936 138 189 465 51
Cuts at: 5261 6012 6079 6554 6593 6804 6878 1870
Size: 751 67 475 39 211 74 4545
Fragments arranged by size:
4545 1454 936 751 475 465 211 189
138 85 74 73 67 51 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4310 4343 4310
Size: 33 9520
BamHI G'GATC_C
Cuts at: 3472 4552 6883 7250 3472
Size: 1080 2331 367 5775
Fragments arranged by size:
5775 2331 1080 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 6350 6498 6874 8329
Size: 325 217 627 245 4318 148 376 1455
Cuts at: 8329 618
Size: 1842
Fragments arranged by size:
4318 1842 1455 627 376 325 245 217
148
BanII G_rGCy'C
Cuts at: 1391 2528 3796 4893 4974 6080 6418 6600 6872
Size: 1137 1268 1097 81 1106 338 182 272
Cuts at: 6872 1391
Size: 4072
Fragments arranged by size:
4072 1268 1137 1106 1097 338 272 182
81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2598 3936 4592 928
Size: 971 699 1338 656 5889
Fragments arranged by size:
5889 1338 971 699 656
BbvCI CC'TCA_GC
Cuts at: 5759 6817 5759
Size: 1058 8495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 7744 7778 1903
Size: 34 50 34 5723 34 3678
Fragments arranged by size:
5723 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7445 8655 7445
Size: 1210 8343
BclI T'GATC_A
Cuts at: 1548 3585 3756 4612 1548
Size: 2037 171 856 6489
Fragments arranged by size:
6489 2037 856 171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2051 4700
Size: 107 122 71 631 605 337 42 2649
Cuts at: 4700 8164 136
Size: 3464 1525
Fragments arranged by size:
3464 2649 1525 631 605 337 122 107
71 42
BglII A'GATC_T
Cuts at: 2878 3519 3882 4998 6159 9548 2878
Size: 641 363 1116 1161 3389 2883
Fragments arranged by size:
3389 2883 1161 1116 641 363
BlpI GC'TnA_GC
Cuts at: 2445 6026 6970 2445
Size: 3581 944 5028
Fragments arranged by size:
5028 3581 944
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2528 4696 5888 6418 6667 7614 8770
Size: 247 491 2168 1192 530 249 947 1156
Cuts at: 8770 1790
Size: 2573
Fragments arranged by size:
2573 2168 1192 1156 947 530 491 249
247
BmgBI CAC'GTC
Cuts at: 1318 4428 5653 1318
Size: 3110 1225 5218
Fragments arranged by size:
5218 3110 1225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4540 6541
Size: 416 53 83 399 24 2054 1069 2001
Cuts at: 6541 7284 8242 442
Size: 743 958 1753
Fragments arranged by size:
2054 2001 1753 1069 958 743 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9553
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5990 6832 5990
Size: 842 8711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4460 4589 4757 5082 5155 5819 5985 6176
Size: 3221 129 168 325 73 664 166 191
Cuts at: 6176 8213 1239
Size: 2037 2579
Fragments arranged by size:
3221 2579 2037 664 325 191 168 166
129 73
Bpu10I CC'TnA_GC
Cuts at: 4968 5759 6817 6846 4968
Size: 791 1058 29 7675
Fragments arranged by size:
7675 1058 791 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7678 8543 8841 7678
Size: 865 298 8390
Fragments arranged by size:
8390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3593 5603 8216 882
Size: 617 2094 2010 2613 2219
Fragments arranged by size:
2613 2219 2094 2010 617
BsaAI yAC'GTr
Cuts at: 493 1204 2210 6001 7344 493
Size: 711 1006 3791 1343 2702
Fragments arranged by size:
3791 2702 1343 1006 711
BsaBI GATnn'nnATC
Cuts at: 3524 5402 9547 3524
Size: 1878 4145 3530
Fragments arranged by size:
4145 3530 1878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2320 3737 3966 7360
Size: 53 83 186 936 787 1417 229 3394
Cuts at: 7360 7742 8377 275
Size: 382 635 1451
Fragments arranged by size:
3394 1451 1417 936 787 635 382 229
186 83 53
BsaWI w'CCGG_w
Cuts at: 4923 6090 7986 8384 8658 8805 9216 4923
Size: 1167 1896 398 274 147 411 5260
Fragments arranged by size:
5260 1896 1167 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3037 3067 3595 3625 4200
Size: 30 165 30 1634 30 528 30 575
Cuts at: 4200 4230 5537 5567 5648 5678 5804 5834 6167
Size: 30 1307 30 81 30 126 30 333
Cuts at: 6167 6197 1178
Size: 30 4534
Fragments arranged by size:
4534 1634 1307 575 528 333 165 126
81 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2437 2798 2981 5159 6848 1186
Size: 1057 194 361 183 2178 1689 3891
Fragments arranged by size:
3891 2178 1689 1057 361 194 183
BseYI C'CCAG_C
Cuts at: 2023 3213 3767 3778 4400 4650 5603 8756 9252
Size: 1190 554 11 622 250 953 3153 496
Cuts at: 9252 2023
Size: 2324
Fragments arranged by size:
3153 2324 1190 953 622 554 496 250
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2834 5167 5192 5981 1163
Size: 573 1098 2333 25 789 4735
Fragments arranged by size:
4735 2333 1098 789 573 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6400 7003 7764 8792 1540
Size: 275 186 4399 603 761 1028 2301
Fragments arranged by size:
4399 2301 1028 761 603 275 186
BsiHKAI G_wGCw'C
Cuts at: 2293 4920 5888 6080 6624 7614 7699 8770 2293
Size: 2627 968 192 544 990 85 1071 3076
Fragments arranged by size:
3076 2627 1071 990 968 544 192 85
BsmI GAATG_Cn'
Cuts at: 3063 3820 7064 7157 3063
Size: 757 3244 93 5459
Fragments arranged by size:
5459 3244 757 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2608 2832 2968 5643 2608
Size: 224 136 2675 6518
Fragments arranged by size:
6518 2675 224 136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2293 2528 3796 4696 4893 4920
Size: 399 247 256 235 1268 900 197 27
Cuts at: 4920 4974 5888 6080 6418 6600 6624 6667 6872
Size: 54 914 192 338 182 24 43 205
Cuts at: 6872 7614 7699 8770 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1268 1071 914 900 742 399 338
256 247 235 205 197 192 182 85
54 43 27 24
BspEI T'CCGG_A
Cuts at: 6090 9216 6090
Size: 3126 6427
BspHI T'CATG_A
Cuts at: 3033 7442 9172 3033
Size: 4409 1730 3414
Fragments arranged by size:
4409 3414 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3236 3673 4833 5133 5214 6132 6718 6908
Size: 2392 437 1160 300 81 918 586 190
Cuts at: 6908 844
Size: 3489
Fragments arranged by size:
3489 2392 1160 918 586 437 300 190
81
BsrBI CCG'CTC
Cuts at: 1394 1823 4069 6669 7440 1394
Size: 429 2246 2600 771 3507
Fragments arranged by size:
3507 2600 2246 771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6742 8048 8222 65
Size: 1183 5494 1306 174 1396
Fragments arranged by size:
5494 1396 1306 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 3275 3954 5778 5906 6446 6519
Size: 110 198 1267 679 1824 128 540 73
Cuts at: 6519 6693 6723 8197 8384 1700
Size: 174 30 1474 187 2869
Fragments arranged by size:
2869 1824 1474 1267 679 540 198 187
174 128 110 73 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9553
BssSI C'ACGA_G
Cuts at: 7613 8625 7613
Size: 1012 8541
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3069 6456 794
Size: 2275 3387 3891
Fragments arranged by size:
3891 3387 2275
BstBI TT'CG_AA
Cuts at: 8357 8357
Size: 9553
BstEII G'GTnAC_C
Cuts at: 1184 3254 1184
Size: 2070 7483
BstXI CCAn_nnnn'nTGG
Cuts at: 3871 4735 5950 6536 6772 3871
Size: 864 1215 586 236 6652
Fragments arranged by size:
6652 1215 864 586 236
BstYI r'GATC_y
Cuts at: 1972 2878 3472 3519 3882 4552 4998 5296 6159
Size: 906 594 47 363 670 446 298 863
Cuts at: 6159 6883 7250 7635 7652 9104 9190 9202 9548
Size: 724 367 385 17 1452 86 12 346
Cuts at: 9548 1972
Size: 1977
Fragments arranged by size:
1977 1452 906 863 724 670 594 446
385 367 363 346 298 86 47 17
12
BstZ17I GTA'TAC
Cuts at: 6375 6375
Size: 9553
Bsu36I CC'TnA_GG
Cuts at: 2544 4833 6230 2544
Size: 2289 1397 5867
Fragments arranged by size:
5867 2289 1397
BtgI C'CryG_G
Cuts at: 513 1065 3603 4536 4880 4893 5151 5187 5232
Size: 552 2538 933 344 13 258 36 45
Cuts at: 5232 5623 5835 5955 6354 6421 6600 513
Size: 391 212 120 399 67 179 3466
Fragments arranged by size:
3466 2538 933 552 399 391 344 258
212 179 120 67 45 36 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3973 487
Size: 683 56 2747 6067
Fragments arranged by size:
6067 2747 683 56
BtsI GCAGTG_nn'
Cuts at: 4045 5993 7069 7863 7883 4045
Size: 1948 1076 794 20 5715
Fragments arranged by size:
5715 1948 1076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9553
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9518
DraI TTT'AAA
Cuts at: 2262 6857 2262
Size: 4595 4958
DraIII CAC_nnn'GTG
Cuts at: 5496 6643 5496
Size: 1147 8406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2742 8560 809
Size: 507 1426 5818 1802
Fragments arranged by size:
5818 1802 1426 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2052 4086
Size: 54 1425 50 275 18 213 9 2034
Cuts at: 4086 4984 5626 5952 6357 6397 6756 6795 7000
Size: 898 642 326 405 40 359 39 205
Cuts at: 7000 7889 9518 8
Size: 889 1629 43
Fragments arranged by size:
2034 1629 1425 898 889 642 405 359
326 275 213 205 54 50 43 40
39 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 6397 7000 1537
Size: 275 4585 603 4090
Fragments arranged by size:
4585 4090 603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2544 2606 2966 3308 4229 5009 6704 7483
Size: 1191 62 360 342 921 780 1695 779
Cuts at: 7483 1353
Size: 3423
Fragments arranged by size:
3423 1695 1191 921 780 779 360 342
62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8140 8514 8660 387
Size: 1064 138 6551 374 146 1280
Fragments arranged by size:
6551 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6078 6078
Size: 9553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2798 3217 3424 4460 4589 4757 5082
Size: 393 1166 419 207 1036 129 168 325
Cuts at: 5082 5155 5609 5674 5819 5864 5985 6176 6556
Size: 73 454 65 145 45 121 191 380
Cuts at: 6556 7616 8213 9000 9268 1239
Size: 1060 597 787 268 1524
Fragments arranged by size:
1524 1166 1060 1036 787 597 454 419
393 380 325 268 207 191 168 145
129 121 73 65 45
EcoNI CCTnn'n_nnAGG
Cuts at: 4568 6128 4568
Size: 1560 7993
EcoO109I rG'GnC_Cy
Cuts at: 2524 2525 3427 3580 3751 3845 4732 4750 4765
Size: 1 902 153 171 94 887 18 15
Cuts at: 4765 4903 5203 5515 6083 6414 6415 2524
Size: 138 300 312 568 331 1 5662
Fragments arranged by size:
5662 902 887 568 331 312 300 171
153 138 94 18 15 1 1
EcoRI G'AATT_C
Cuts at: 6862 7240 6862
Size: 378 9175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4609 4609
Size: 9553
FspI TGC'GCA
Cuts at: 8059 8059
Size: 9553
HaeII r_GCGC'y
Cuts at: 1678 3209 3955 4349 5833 8700 1678
Size: 1531 746 394 1484 2867 2531
Fragments arranged by size:
2867 2531 1531 1484 746 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3037 3069 3146 3178 3329
Size: 32 135 32 1465 32 77 32 151
Cuts at: 3329 3361 3951 3983 4127 4159 4200 4232 4277
Size: 32 590 32 144 32 41 32 45
Cuts at: 4277 4298 4309 4330 4544 4576 4990 5022 6067
Size: 21 11 21 214 32 414 32 1045
Cuts at: 6067 6099 6101 6133 6167 6199 8194 8226 8268
Size: 32 2 32 34 32 1995 32 42
Cuts at: 8268 8300 1373
Size: 32 2626
Fragments arranged by size:
2626 1995 1465 1045 590 414 214 151
144 135 77 45 42 41 34 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 21 21
11 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2870 5059 6897 7143 669
Size: 988 281 932 2189 1838 246 3079
Fragments arranged by size:
3079 2189 1838 988 932 281 246
HindIII A'AGCT_T
Cuts at: 748 5287 6782 748
Size: 4539 1495 3519
Fragments arranged by size:
4539 3519 1495
HpaI GTT'AAC
Cuts at: 7143 7143
Size: 9553
KpnI G_GTAC'C
Cuts at: 6878 6878
Size: 9553
MfeI C'AATT_G
Cuts at: 7152 7152
Size: 9553
MluI A'CGCG_T
Cuts at: 9429 9429
Size: 9553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3588 4186 4410 4959 5439 6144 8667 8851
Size: 2283 598 224 549 480 705 2523 184
Cuts at: 8851 1305
Size: 2007
Fragments arranged by size:
2523 2283 2007 705 598 549 480 224
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4088 6359 6758 6797 10
Size: 54 1425 343 2256 2271 399 39 2766
Fragments arranged by size:
2766 2271 2256 1425 399 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 3467 5507 6101 6332 7511 7870 8029
Size: 648 2301 2040 594 231 1179 359 159
Cuts at: 8029 518
Size: 2042
Fragments arranged by size:
2301 2042 2040 1179 648 594 359 231
159
MspA1I CmG'CkG
Cuts at: 1606 4324 4603 5181 5625 5632 5694 5859 5957
Size: 2718 279 578 444 7 62 165 98
Cuts at: 5957 6025 6969 7646 8794 9039 1606
Size: 68 944 677 1148 245 2120
Fragments arranged by size:
2718 2120 1148 944 677 578 444 279
245 165 98 68 62 7
NaeI GCC'GGC
Cuts at: 2010 3956 6448 2010
Size: 1946 2492 5115
Fragments arranged by size:
5115 2492 1946
NcoI C'CATG_G
Cuts at: 513 1065 4536 5232 5835 6354 6600 513
Size: 552 3471 696 603 519 246 3466
Fragments arranged by size:
3471 3466 696 603 552 519 246
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9553
NgoMIV G'CCGG_C
Cuts at: 2008 3954 6446 2008
Size: 1946 2492 5115
Fragments arranged by size:
5115 2492 1946
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9553
NotI GC'GGCC_GC
Cuts at: 7000 7000
Size: 9553
NspI r_CATG'y
Cuts at: 1132 1229 3748 4474 6280 6911 8456 1132
Size: 97 2519 726 1806 631 1545 2229
Fragments arranged by size:
2519 2229 1806 1545 726 631 97
PasI CC'CwG_GG
Cuts at: 1348 4728 6299 6673 1348
Size: 3380 1571 374 4228
Fragments arranged by size:
4228 3380 1571 374
PciI A'CATG_T
Cuts at: 8452 8452
Size: 9553
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9553
PfoI T'CCnGG_A
Cuts at: 4734 5796 5985 6711 4734
Size: 1062 189 726 7576
Fragments arranged by size:
7576 1062 726 189
PmeI GTTT'AAAC
Cuts at: 6857 6857
Size: 9553
PmlI CAC'GTG
Cuts at: 6001 6001
Size: 9553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5804 5836 7622 7654 9135 9167 5804
Size: 32 1786 32 1481 32 6190
Fragments arranged by size:
6190 1786 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3580 3751 3845 4732 4750 4765 4903 5203 5515
Size: 171 94 887 18 15 138 300 312
Cuts at: 5515 6083 3580
Size: 568 7050
Fragments arranged by size:
7050 887 568 312 300 171 138 94
18 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9553
PsiI TTA'TAA
Cuts at: 7123 7123
Size: 9553
PspOMI G'GGCC_C
Cuts at: 2524 6414 2524
Size: 3890 5663
PspXI vC'TCGA_Gb
Cuts at: 4745 5261 6554 4745
Size: 516 1293 7744
Fragments arranged by size:
7744 1293 516
PstI C_TGCA'G
Cuts at: 830 2662 3555 5904 6905 830
Size: 1832 893 2349 1001 3478
Fragments arranged by size:
3478 2349 1832 1001 893
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9553
PvuII CAG'CTG
Cuts at: 4603 6025 4603
Size: 1422 8131
SacI G_AGCT'C
Cuts at: 6080 6080
Size: 9553
SacII CC_GC'GG
Cuts at: 5626 5958 5626
Size: 332 9221
SalI G'TCGA_C
Cuts at: 1936 6895 1936
Size: 4959 4594
SanDI GG'GwC_CC
Cuts at: 3845 5515 3845
Size: 1670 7883
SapI GCTCTTCn'nnn_
Cuts at: 5009 6704 5009
Size: 1695 7858
SbfI CC_TGCA'GG
Cuts at: 6905 6905
Size: 9553
ScaI AGT'ACT
Cuts at: 7801 9513 7801
Size: 1712 7841
SexAI A'CCwGG_T
Cuts at: 6068 6068
Size: 9553
SfcI C'TryA_G
Cuts at: 826 1045 2658 3551 5068 5900 6901 8036 8717
Size: 219 1613 893 1517 832 1001 1135 681
Cuts at: 8717 8908 826
Size: 191 1471
Fragments arranged by size:
1613 1517 1471 1135 1001 893 832 681
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2051 2051
Size: 9553
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9553
SgrAI Cr'CCGG_yG
Cuts at: 1700 3954 1700
Size: 2254 7299
SmaI CCC'GGG
Cuts at: 1872 2030 6014 6880 1872
Size: 158 3984 866 4545
Fragments arranged by size:
4545 3984 866 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4745 5261 6554 7657 8558 8820
Size: 197 926 2802 516 1293 1103 901 262
Cuts at: 8820 820
Size: 1553
Fragments arranged by size:
2802 1553 1293 1103 926 901 516 262
197
SnaBI TAC'GTA
Cuts at: 493 2210 7344 493
Size: 1717 5134 2702
Fragments arranged by size:
5134 2702 1717
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9553
SphI G_CATG'C
Cuts at: 1132 3748 6280 6911 1132
Size: 2616 2532 631 3774
Fragments arranged by size:
3774 2616 2532 631
SspI AAT'ATT
Cuts at: 5 52 7477 5
Size: 47 7425 2081
Fragments arranged by size:
7425 2081 47
StuI AGG'CCT
Cuts at: 1517 4838 5074 5267 1517
Size: 3321 236 193 5803
Fragments arranged by size:
5803 3321 236 193
StyI C'CwwG_G
Cuts at: 513 1065 2277 4536 5232 5835 6291 6354 6600
Size: 552 1212 2259 696 603 456 63 246
Cuts at: 6600 6992 513
Size: 392 3074
Fragments arranged by size:
3074 2259 1212 696 603 552 456 392
246 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2779 3129 4162 5099 6642 7580 7597
Size: 1255 1199 350 1033 937 1543 938 17
Cuts at: 7597 7750 7935 8380 325
Size: 153 185 445 1498
Fragments arranged by size:
1543 1498 1255 1199 1033 938 937 445
350 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3240 5226 7799
Size: 274 80 33 51 1443 1263 1986 2573
Cuts at: 7799 9511 96
Size: 1712 138
Fragments arranged by size:
2573 1986 1712 1443 1263 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2031 2063 3595 3627 5648 5680 9513 9545 2031
Size: 32 1532 32 2021 32 3833 32 2039
Fragments arranged by size:
3833 2039 2021 1532 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2072 4289 4412 1316
Size: 756 2217 123 6457
Fragments arranged by size:
6457 2217 756 123
XbaI T'CTAG_A
Cuts at: 2323 6889 2323
Size: 4566 4987
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3868 6306 3868
Size: 2438 7115
XhoI C'TCGA_G
Cuts at: 1943 4745 5261 6554 1943
Size: 2802 516 1293 4942
Fragments arranged by size:
4942 2802 1293 516
XmaI C'CCGG_G
Cuts at: 1870 2028 6012 6878 1870
Size: 158 3984 866 4545
Fragments arranged by size:
4545 3984 866 158
XmnI GAAnn'nnTTC
Cuts at: 3462 3635 5705 7682 3462
Size: 173 2070 1977 5333
Fragments arranged by size:
5333 2070 1977 173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2321 7361 276
Size: 53 83 186 936 787 5040 2468
Fragments arranged by size:
5040 2468 936 787 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BfrBI BsiWI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PsrI RsrII SfoI
SrfI SwaI