Restriction map for
pFN21AE9670
(Circular) (Six-base) MAPSORT of: pFN21AE9670.seq Check: 4286 from: 1 to: 14395
pFN21AE9670 14395 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5176 5344 10159 10573 5176
Size: 168 4815 414 8998
Fragments arranged by size:
8998 4815 414 168
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11575 12205 278
Size: 53 83 186 936 10039 630 2468
Fragments arranged by size:
10039 2468 936 630 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6911 11738 1937
Size: 4974 4827 4594
Fragments arranged by size:
4974 4827 4594
Acc65I G'GTAC_C
Cuts at: 6946 7177 11716 6946
Size: 231 4539 9625
Fragments arranged by size:
9625 4539 231
AclI AA'CG_TT
Cuts at: 1563 4066 12522 12895 1563
Size: 2503 8456 373 3063
Fragments arranged by size:
8456 3063 2503 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 3120 3132 3609 4756 6150 6174 7338
Size: 755 733 12 477 1147 1394 24 1164
Cuts at: 7338 7785 8703 9333 9936 9980 10104 11547 12458
Size: 447 918 630 603 44 124 1443 911
Cuts at: 12458 13842 14110 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1443 1394 1384 1164 1147 918 911
755 733 630 603 477 447 268 124
44 24 12
AfeI AGC'GCT
Cuts at: 4277 8945 4277
Size: 4668 9727
AflII C'TTAA_G
Cuts at: 820 1017 8107 820
Size: 197 7090 7108
Fragments arranged by size:
7108 7090 197
AflIII A'CryG_T
Cuts at: 1203 6601 13294 14271 1203
Size: 5398 6693 977 1327
Fragments arranged by size:
6693 5398 1327 977
AgeI A'CCGG_T
Cuts at: 4735 6943 13226 4735
Size: 2208 6283 5904
Fragments arranged by size:
6283 5904 2208
AhdI GACnn_n'nnGTC
Cuts at: 2743 6567 7909 13124 2743
Size: 3824 1342 5215 4014
Fragments arranged by size:
5215 4014 3824 1342
AleI CACnn'nnGTG
Cuts at: 1166 4034 5477 6113 10334 1166
Size: 2868 1443 636 4221 5227
Fragments arranged by size:
5227 4221 2868 1443 636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3372 8925 10352 1184
Size: 2188 5553 1427 5227
Fragments arranged by size:
5553 5227 2188 1427
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7701 7733 9047 9079 7701
Size: 32 1314 32 13017
Fragments arranged by size:
13017 1314 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3380 3500 3776 5093 6857 7045 7198 7240 7366
Size: 120 276 1317 1764 188 153 42 126
Cuts at: 7366 8399 8421 9392 9770 9884 9983 10622 10691
Size: 1033 22 971 378 114 99 639 69
Cuts at: 10691 10721 12138 13710 3380
Size: 30 1417 1572 4065
Fragments arranged by size:
4065 1764 1572 1417 1317 1033 971 639
378 276 188 153 126 120 114 99
69 42 30 22
ApaI G_GGCC'C
Cuts at: 2264 2554 3450 3729 5066 5178 6413 7289 7403
Size: 290 896 279 1337 112 1235 876 114
Cuts at: 7403 7744 10428 10752 11434 2264
Size: 341 2684 324 682 5225
Fragments arranged by size:
5225 2684 1337 1235 896 876 682 341
324 290 279 114 112
ApaLI G'TGCA_C
Cuts at: 5720 12452 13608 5720
Size: 6732 1156 6507
Fragments arranged by size:
6732 6507 1156
ApoI r'AATT_y
Cuts at: 1493 8813 8957 11704 11930 12082 14191 1493
Size: 7320 144 2747 226 152 2109 1697
Fragments arranged by size:
7320 2747 2109 1697 226 152 144
AseI AT'TA_AT
Cuts at: 160 1032 12949 160
Size: 872 11917 1606
Fragments arranged by size:
11917 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14395
AvaI C'yCGr_G
Cuts at: 1870 1943 3087 3315 5955 6087 7021 8052 8478
Size: 73 1144 228 2640 132 934 1031 426
Cuts at: 8478 8892 9210 10366 10526 11102 11720 1870
Size: 414 318 1156 160 576 618 4545
Fragments arranged by size:
4545 2640 1156 1144 1031 934 618 576
426 414 318 228 160 132 73
AvrII C'CTAG_G
Cuts at: 9326 9326
Size: 14395
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7500 7533 7500
Size: 33 14362
BamHI G'GATC_C
Cuts at: 11725 12092 11725
Size: 367 14028
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2383 2517 4071 4902
Size: 325 217 627 414 182 134 1554 831
Cuts at: 4902 5053 5663 5896 6445 6484 6652 6841 6946
Size: 151 610 233 549 39 168 189 105
Cuts at: 6946 7177 7207 7490 7747 7957 9134 9874 10642
Size: 231 30 283 257 210 1177 740 768
Cuts at: 10642 11716 13171 618
Size: 1074 1455 1842
Fragments arranged by size:
1842 1554 1455 1177 1074 831 768 740
627 610 549 414 325 283 257 233
231 217 210 189 182 168 151 134
105 39 30
BanII G_rGCy'C
Cuts at: 1391 2264 2394 2554 3123 3450 3729 4108 5066
Size: 873 130 160 569 327 279 379 958
Cuts at: 5066 5100 5178 5357 6011 6094 6413 7028 7289
Size: 34 78 179 654 83 319 615 261
Cuts at: 7289 7379 7403 7744 8046 8059 9000 9211 9966
Size: 90 24 341 302 13 941 211 755
Cuts at: 9966 10428 10752 11434 11714 1391
Size: 462 324 682 280 4072
Fragments arranged by size:
4072 958 941 873 755 682 654 615
569 462 379 341 327 324 319 302
280 279 261 211 179 160 130 90
83 78 34 24 13
BbeI G_GCGC'C
Cuts at: 5057 5057
Size: 14395
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3956 4384 6543 9191 928
Size: 971 2057 428 2159 2648 6132
Fragments arranged by size:
6132 2648 2159 2057 971 428
BbvCI CC'TCA_GC
Cuts at: 2034 2812 3027 3261 5870 6934 8874 9888 9973
Size: 778 215 234 2609 1064 1940 1014 85
Cuts at: 9973 10092 2034
Size: 119 6337
Fragments arranged by size:
6337 2609 1940 1064 1014 778 234 215
119 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 11350 11384 12586 12620 1903
Size: 34 50 34 9329 34 1202 34 3678
Fragments arranged by size:
9329 3678 1202 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3241 7528 12287 13497 3241
Size: 4287 4759 1210 4139
Fragments arranged by size:
4759 4287 4139 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 14395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3983 5582 5774
Size: 107 122 71 631 605 2311 1599 192
Cuts at: 5774 7812 7874 8780 10261 13006 136
Size: 2038 62 906 1481 2745 1525
Fragments arranged by size:
2745 2311 2038 1599 1525 1481 906 631
605 192 122 107 71 62
BglII A'GATC_T
Cuts at: 9754 14390 9754
Size: 4636 9759
BlpI GC'TnA_GC
Cuts at: 2337 3302 8802 8898 11812 2337
Size: 965 5500 96 2914 4920
Fragments arranged by size:
5500 4920 2914 965 96
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2264 2554 3450 3729 4076 5066 5178
Size: 424 50 290 896 279 347 990 112
Cuts at: 5178 5668 5724 6413 7289 7403 7744 8673 9609
Size: 490 56 689 876 114 341 929 936
Cuts at: 9609 9670 9747 10428 10752 11434 12456 13612 1790
Size: 61 77 681 324 682 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 990 936 929 896 876
689 682 681 490 424 347 341 324
290 279 114 112 77 61 56 50
BmgBI CAC'GTC
Cuts at: 1318 8009 1318
Size: 6691 7704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2036 2422 4012
Size: 416 53 83 399 24 619 386 1590
Cuts at: 4012 4057 5066 5117 5336 7232 7379 8089 9380
Size: 45 1009 51 219 1896 147 710 1291
Cuts at: 9380 9392 10679 11429 12126 13084 442
Size: 12 1287 750 697 958 1753
Fragments arranged by size:
1896 1753 1590 1291 1287 1009 958 750
710 697 619 416 399 386 219 147
83 53 51 45 24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 14395
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4441 9014 10565 10663 4441
Size: 4573 1551 98 8173
Fragments arranged by size:
8173 4573 1551 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2169 2237 2641 2758 2806 3705 3770 4802
Size: 930 68 404 117 48 899 65 1032
Cuts at: 4802 5070 5352 6687 6711 6792 6900 7146 7170
Size: 268 282 1335 24 81 108 246 24
Cuts at: 7170 7236 7254 7263 7302 7691 8666 8747 8984
Size: 66 18 9 39 389 975 81 237
Cuts at: 8984 8996 9327 9531 9669 9716 9980 10028 10042
Size: 12 331 204 138 47 264 48 14
Cuts at: 10042 10596 13055 1239
Size: 554 2459 2579
Fragments arranged by size:
2579 2459 1335 1032 975 930 899 554
404 389 331 282 268 264 246 237
204 138 117 108 81 81 68 66
65 48 48 47 39 24 24 18
14 12 9
Bpu10I CC'TnA_GC
Cuts at: 2034 2460 2812 3027 3261 4793 5010 5094 5351
Size: 426 352 215 234 1532 217 84 257
Cuts at: 5351 5870 6708 6934 8442 8874 9888 9973 10092
Size: 519 838 226 1508 432 1014 85 119
Cuts at: 10092 2034
Size: 6337
Fragments arranged by size:
6337 1532 1508 1014 838 519 432 426
352 257 234 226 217 215 119 85
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2145 2687 4109 6113 7393 8153 9207 9664 12520
Size: 542 1422 2004 1280 760 1054 457 2856
Cuts at: 12520 13385 13683 2145
Size: 865 298 2857
Fragments arranged by size:
2857 2856 2004 1422 1280 1054 865 760
542 457 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 10351 10614 10694 11341 11385 13058 882
Size: 617 8852 263 80 647 44 1673 2219
Fragments arranged by size:
8852 2219 1673 647 617 263 80 44
BsaAI yAC'GTr
Cuts at: 493 1204 6211 12186 493
Size: 711 5007 5975 2702
Fragments arranged by size:
5975 5007 2702 711
BsaBI GATnn'nnATC
Cuts at: 4809 14389 4809
Size: 9580 4815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3827 4312 5054 7624
Size: 53 83 186 936 2294 485 742 2570
Cuts at: 7624 10168 11572 12202 12584 13219 275
Size: 2544 1404 630 382 635 1451
Fragments arranged by size:
2570 2544 2294 1451 1404 936 742 635
630 485 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3510 4735 6832 6886 6943 12828 13226 13500 13647
Size: 1225 2097 54 57 5885 398 274 147
Cuts at: 13647 14058 3510
Size: 411 3847
Fragments arranged by size:
5885 3847 2097 1225 411 398 274 147
57 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4043 4073 4302 4315 4332
Size: 30 165 30 2640 30 229 13 17
Cuts at: 4332 4345 4346 4376 5407 5437 7300 7330 8353
Size: 13 1 30 1031 30 1863 30 1023
Cuts at: 8353 8383 9047 9077 9493 9523 9529 9559 1178
Size: 30 664 30 416 30 6 30 6014
Fragments arranged by size:
6014 2640 1863 1031 1023 664 416 229
165 30 30 30 30 30 30 30
30 30 30 17 13 13 6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2540 2576 3353 3459 3557 3647 4280 5073
Size: 1354 36 777 106 98 90 633 793
Cuts at: 5073 5365 6192 6253 6804 6807 6816 8537 8702
Size: 292 827 61 551 3 9 1721 165
Cuts at: 8702 8897 8993 9008 9071 9095 9098 9101 9447
Size: 195 96 15 63 24 3 3 346
Cuts at: 9447 9498 9570 9718 9938 10152 11528 11555 11558
Size: 51 72 148 220 214 1376 27 3
Cuts at: 11558 1186
Size: 4023
Fragments arranged by size:
4023 1721 1376 1354 827 793 777 633
551 346 292 220 214 195 165 148
106 98 96 90 72 63 61 51
36 27 24 15 9 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2116 4076 5106 5356 5843 5989 6352 6412 6457
Size: 1960 1030 250 487 146 363 60 45
Cuts at: 6457 6631 7057 7288 7294 7348 7432 7450 7577
Size: 174 426 231 6 54 84 18 127
Cuts at: 7577 7832 7890 9101 9824 10467 10501 10622 10752
Size: 255 58 1211 723 643 34 121 130
Cuts at: 10752 10767 11060 13598 14094 2116
Size: 15 293 2538 496 2417
Fragments arranged by size:
2538 2417 1960 1211 1030 723 643 496
487 426 363 293 255 250 231 174
146 130 127 121 84 60 58 54
45 34 18 15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5481 7379 8790 9484 9804 1163
Size: 573 3745 1898 1411 694 320 5754
Fragments arranged by size:
5754 3745 1898 1411 694 573 320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4736 7407 11845 12606 13634 1540
Size: 275 186 2735 2671 4438 761 1028 2301
Fragments arranged by size:
4438 2735 2671 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5100 5724 6011 8046 8193 9000 9966 10546 12456
Size: 624 287 2035 147 807 966 580 1910
Cuts at: 12456 12541 13612 5100
Size: 85 1071 5883
Fragments arranged by size:
5883 2035 1910 1071 966 807 624 580
287 147 85
BsmI GAATG_Cn'
Cuts at: 3921 8220 11235 11906 11999 3921
Size: 4299 3015 671 93 6317
Fragments arranged by size:
6317 4299 3015 671 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4303 10530 4303
Size: 6227 8168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2264 2394 2554 3123 3450 3729
Size: 399 424 50 130 160 569 327 279
Cuts at: 3729 4076 4108 5066 5100 5178 5357 5668 5724
Size: 347 32 958 34 78 179 311 56
Cuts at: 5724 6011 6094 6413 7028 7289 7379 7403 7744
Size: 287 83 319 615 261 90 24 341
Cuts at: 7744 8046 8059 8193 8673 9000 9211 9609 9670
Size: 302 13 134 480 327 211 398 61
Cuts at: 9670 9747 9966 10428 10546 10752 11434 11714 12456
Size: 77 219 462 118 206 682 280 742
Cuts at: 12456 12541 13612 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 958 742 682 615 569 480
462 424 399 398 347 341 327 327
319 311 302 287 280 279 261 219
211 206 179 160 134 130 118 90
85 83 78 77 61 56 50 34
32 24 13
BspEI T'CCGG_A
Cuts at: 14058 14058
Size: 14395
BspHI T'CATG_A
Cuts at: 3073 4665 12284 14014 3073
Size: 1592 7619 1730 3454
Fragments arranged by size:
7619 3454 1730 1592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2703 5176 5344 8271 10159 10573 11750 844
Size: 1859 2473 168 2927 1888 414 1177 3489
Fragments arranged by size:
3489 2927 2473 1888 1859 1177 414 168
BsrBI CCG'CTC
Cuts at: 1394 1823 7496 12282 1394
Size: 429 5673 4786 3507
Fragments arranged by size:
5673 4786 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2632 2944 3762 7559 9958 10479 12890
Size: 1183 1384 312 818 3797 2399 521 2411
Cuts at: 12890 13064 65
Size: 174 1396
Fragments arranged by size:
3797 2411 2399 1396 1384 1183 818 521
312 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 6057 6943 8862 9141 11586 13039
Size: 110 2925 1322 886 1919 279 2445 1453
Cuts at: 13039 13226 1700
Size: 187 2869
Fragments arranged by size:
2925 2869 2445 1919 1453 1322 886 279
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 14395
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 14395
BssSI C'ACGA_G
Cuts at: 2522 5726 9183 10839 12455 13467 2522
Size: 3204 3457 1656 1616 1012 3450
Fragments arranged by size:
3457 3450 3204 1656 1616 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6737 7490 8340 9368 794
Size: 5943 753 850 1028 5821
Fragments arranged by size:
5943 5821 1028 850 753
BstBI TT'CG_AA
Cuts at: 10246 13199 10246
Size: 2953 11442
BstEII G'GTnAC_C
Cuts at: 1184 3892 4738 6569 1184
Size: 2708 846 1831 9010
Fragments arranged by size:
9010 2708 1831 846
BstXI CCAn_nnnn'nTGG
Cuts at: 3875 6763 7995 10760 11263 3875
Size: 2888 1232 2765 503 7007
Fragments arranged by size:
7007 2888 2765 1232 503
BstYI r'GATC_y
Cuts at: 1972 8276 9754 9777 10717 11725 12092 12477 12494
Size: 6304 1478 23 940 1008 367 385 17
Cuts at: 12494 13946 14032 14044 14390 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
6304 1977 1478 1452 1008 940 385 367
346 86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 2379 3126 3471 5545 9651 10137 11499 11533 2379
Size: 747 345 2074 4106 486 1362 34 5241
Fragments arranged by size:
5241 4106 2074 1362 747 486 345 34
BtgI C'CryG_G
Cuts at: 513 1065 2595 3294 3333 3450 4800 5048 5454
Size: 552 1530 699 39 117 1350 248 406
Cuts at: 5454 5790 5907 6624 7512 8248 8433 11256 513
Size: 336 117 717 888 736 185 2823 3652
Fragments arranged by size:
3652 2823 1530 1350 888 736 717 699
552 406 336 248 185 117 117 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4388 6831 487
Size: 683 56 3162 2443 8051
Fragments arranged by size:
8051 3162 2443 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 3394 3712 5225 5484 7590 8157 10451 10544
Size: 950 318 1513 259 2106 567 2294 93
Cuts at: 10544 10943 10959 11911 12705 12725 2444
Size: 399 16 952 794 20 4114
Fragments arranged by size:
4114 2294 2106 1513 952 950 794 567
399 318 259 93 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 14395
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3968 4003 529
Size: 35 3404 35 10921
Fragments arranged by size:
10921 3404 35 35
DraI TTT'AAA
Cuts at: 4084 11699 4084
Size: 7615 6780
DraIII CAC_nnn'GTG
Cuts at: 5728 8188 5728
Size: 2460 11935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 13402 809
Size: 507 12086 1802
Fragments arranged by size:
12086 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 3807 6055
Size: 54 1425 50 275 18 768 1209 2248
Cuts at: 6055 6531 6669 7404 8251 8470 8571 8703 8860
Size: 476 138 735 847 219 101 132 157
Cuts at: 8860 9711 10214 11842 12731 14360 8
Size: 851 503 1628 889 1629 43
Fragments arranged by size:
2248 1629 1628 1425 1209 889 851 847
768 735 503 476 275 219 157 138
132 101 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7404 11842 1537
Size: 275 5592 4438 4090
Fragments arranged by size:
5592 4438 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2363 2424 2794 2812 2886 2997 3111
Size: 917 93 61 370 18 74 111 114
Cuts at: 3111 3201 3219 3237 3456 3585 3624 3675 6129
Size: 90 18 18 219 129 39 51 2454
Cuts at: 6129 6202 6244 6601 6834 8546 8679 8688 8751
Size: 73 42 357 233 1712 133 9 63
Cuts at: 8751 9006 10023 11015 11237 12325 1353
Size: 255 1017 992 222 1088 3423
Fragments arranged by size:
3423 2454 1712 1088 1017 992 917 370
357 255 233 222 219 133 129 114
111 93 90 74 73 63 61 51
42 39 18 18 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9056 9241 12982 13356 13502 387
Size: 1064 138 7467 185 3741 374 146 1280
Fragments arranged by size:
7467 3741 1280 1064 374 185 146 138
EcoICRI GAG'CTC
Cuts at: 5098 6009 8044 8998 9964 5098
Size: 911 2035 954 966 9529
Fragments arranged by size:
9529 2035 966 954 911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2169 2237 2387 2641 2758 2806 3120
Size: 393 537 68 150 254 117 48 314
Cuts at: 3120 3132 3609 3705 3770 4756 4802 5070 5352
Size: 12 477 96 65 986 46 268 282
Cuts at: 5352 6150 6174 6687 6711 6792 6900 7146 7170
Size: 798 24 513 24 81 108 246 24
Cuts at: 7170 7236 7254 7263 7302 7338 7691 7785 8666
Size: 66 18 9 39 36 353 94 881
Cuts at: 8666 8703 8747 8984 8996 9327 9333 9531 9669
Size: 37 44 237 12 331 6 198 138
Cuts at: 9669 9716 9936 9980 10028 10042 10104 10596 11547
Size: 47 220 44 48 14 62 492 951
Cuts at: 11547 12458 13055 13842 14110 1239
Size: 911 597 787 268 1524
Fragments arranged by size:
1524 986 951 911 881 798 787 597
537 513 492 477 393 353 331 314
282 268 268 254 246 237 220 198
150 138 117 108 96 94 81 68
66 65 62 48 48 47 46 44
44 39 37 36 24 24 24 18
14 12 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3102 3720 4493 5085 5783 8537 3102
Size: 618 773 592 698 2754 8960
Fragments arranged by size:
8960 2754 773 698 618 592
EcoO109I rG'GnC_Cy
Cuts at: 2189 2260 3041 3446 3725 4007 5063 5090 5175
Size: 71 781 405 279 282 1056 27 85
Cuts at: 5175 5278 5289 7130 7740 7741 10020 10142 10232
Size: 103 11 1841 610 1 2279 122 90
Cuts at: 10232 10425 10590 10749 11430 11431 11495 11559 2189
Size: 193 165 159 681 1 64 64 5025
Fragments arranged by size:
5025 2279 1841 1056 781 681 610 405
282 279 193 165 159 122 103 90
85 71 64 64 27 11 1 1
EcoRI G'AATT_C
Cuts at: 11704 12082 11704
Size: 378 14017
EcoRV GAT'ATC
Cuts at: 8764 8764
Size: 14395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3344 3344
Size: 14395
FseI GG_CCGG'CC
Cuts at: 8866 8866
Size: 14395
FspI TGC'GCA
Cuts at: 2504 8090 11630 12901 2504
Size: 5586 3540 1271 3998
Fragments arranged by size:
5586 3998 3540 1271
FspAI rTGC'GCAy
Cuts at: 2504 8090 2504
Size: 5586 8809
HaeII r_GCGC'y
Cuts at: 1678 2470 4228 4279 5057 5509 7427 8947 11640
Size: 792 1758 51 778 452 1918 1520 2693
Cuts at: 11640 13542 1678
Size: 1902 2531
Fragments arranged by size:
2693 2531 1918 1902 1758 1520 792 778
452 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2311 2343 2729 2761 3593
Size: 32 135 32 739 32 386 32 832
Cuts at: 3593 3625 3952 3984 4302 4315 4334 4346 4347
Size: 32 327 32 318 13 19 12 1
Cuts at: 4347 4378 6553 6585 7228 7260 7300 7332 7895
Size: 31 2175 32 643 32 40 32 563
Cuts at: 7895 7927 8178 8210 8873 8905 10007 10039 10871
Size: 32 251 32 663 32 1102 32 832
Cuts at: 10871 10903 13036 13068 13110 13142 1373
Size: 32 2133 32 42 32 2626
Fragments arranged by size:
2626 2175 2133 1102 832 832 739 663
643 563 386 327 318 251 135 42
40 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 31 19 13 12 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4598 4987 6048 6423 11739 11985
Size: 988 281 2660 389 1061 375 5316 246
Cuts at: 11985 669
Size: 3079
Fragments arranged by size:
5316 3079 2660 1061 988 389 375 281
246
HindIII A'AGCT_T
Cuts at: 748 3911 748
Size: 3163 11232
HpaI GTT'AAC
Cuts at: 6048 11985 6048
Size: 5937 8458
KasI G'GCGC_C
Cuts at: 5053 5053
Size: 14395
KpnI G_GTAC'C
Cuts at: 6950 7181 11720 6950
Size: 231 4539 9625
Fragments arranged by size:
9625 4539 231
MfeI C'AATT_G
Cuts at: 8030 11994 8030
Size: 3964 10431
MluI A'CGCG_T
Cuts at: 14271 14271
Size: 14395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 2453 3015 3114 3252 5513 6980 7163
Size: 741 407 562 99 138 2261 1467 183
Cuts at: 7163 7253 7744 10559 10877 11295 13509 13693 1305
Size: 90 491 2815 318 418 2214 184 2007
Fragments arranged by size:
2815 2261 2214 2007 1467 741 562 491
418 407 318 184 183 138 99 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 3809 6533 6671 8253
Size: 54 1425 343 768 1209 2724 138 1582
Cuts at: 8253 8472 8573 8705 9713 10
Size: 219 101 132 1008 4692
Fragments arranged by size:
4692 2724 1582 1425 1209 1008 768 343
219 138 132 101 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3078 3192 4034 4685 5477 6113 8204
Size: 648 1912 114 842 651 792 636 2091
Cuts at: 8204 10334 11261 12353 12712 12871 518
Size: 2130 927 1092 359 159 2042
Fragments arranged by size:
2130 2091 2042 1912 1092 927 842 792
651 648 636 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 2533 2816 3031 3260 3776 4578 4693 4822
Size: 927 283 215 229 516 802 115 129
Cuts at: 4822 5704 6023 6461 7693 7909 8457 8929 9288
Size: 882 319 438 1232 216 548 472 359
Cuts at: 9288 10091 10315 11811 12488 13636 13881 1606
Size: 803 224 1496 677 1148 245 2120
Fragments arranged by size:
2120 1496 1232 1148 927 882 803 802
677 548 516 472 438 359 319 283
245 229 224 216 215 129 115
NaeI GCC'GGC
Cuts at: 6059 8864 11588 6059
Size: 2805 2724 8866
Fragments arranged by size:
8866 2805 2724
NarI GG'CG_CC
Cuts at: 5054 5054
Size: 14395
NcoI C'CATG_G
Cuts at: 513 1065 2595 3294 3333 5907 7512 8248 8433
Size: 552 1530 699 39 2574 1605 736 185
Cuts at: 8433 11256 513
Size: 2823 3652
Fragments arranged by size:
3652 2823 2574 1605 1530 736 699 552
185 39
NdeI CA'TA_TG
Cuts at: 387 387
Size: 14395
NgoMIV G'CCGG_C
Cuts at: 6057 8862 11586 6057
Size: 2805 2724 8866
Fragments arranged by size:
8866 2805 2724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 14395
NotI GC'GGCC_GC
Cuts at: 11842 11842
Size: 14395
NruI TCG'CGA
Cuts at: 4407 4407
Size: 14395
NspI r_CATG'y
Cuts at: 1132 1229 4571 6605 6639 7979 8089 10393 11753
Size: 97 3342 2034 34 1340 110 2304 1360
Cuts at: 11753 13298 1132
Size: 1545 2229
Fragments arranged by size:
3342 2304 2229 2034 1545 1360 1340 110
97 34
PasI CC'CwG_GG
Cuts at: 1348 5059 5272 6227 7126 7396 10691 10856 11137
Size: 3711 213 955 899 270 3295 165 281
Cuts at: 11137 11491 1348
Size: 354 4252
Fragments arranged by size:
4252 3711 3295 955 899 354 281 270
213 165
PciI A'CATG_T
Cuts at: 6601 13294 6601
Size: 6693 7702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3380 5791 7848 9676 10691 11441 1108
Size: 2272 2411 2057 1828 1015 750 4062
Fragments arranged by size:
4062 2411 2272 2057 1828 1015 750
PfoI T'CCnGG_A
Cuts at: 3858 6466 6916 7770 8022 8961 10725 3858
Size: 2608 450 854 252 939 1764 7528
Fragments arranged by size:
7528 2608 1764 939 854 450 252
PmeI GTTT'AAAC
Cuts at: 11699 11699
Size: 14395
PmlI CAC'GTG
Cuts at: 6211 6211
Size: 14395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9047 9079 12464 12496 13977 14009 9047
Size: 32 3385 32 1481 32 9433
Fragments arranged by size:
9433 3385 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4007 5090 5278 5289 7130 10020 10142 11559 4007
Size: 1083 188 11 1841 2890 122 1417 6843
Fragments arranged by size:
6843 2890 1841 1417 1083 188 122 11
PshAI GACnn'nnGTC
Cuts at: 1613 4833 7766 8457 9185 1613
Size: 3220 2933 691 728 6823
Fragments arranged by size:
6823 3220 2933 728 691
PsiI TTA'TAA
Cuts at: 11965 11965
Size: 14395
PspOMI G'GGCC_C
Cuts at: 2260 2550 3446 3725 5062 5174 6409 7285 7399
Size: 290 896 279 1337 112 1235 876 114
Cuts at: 7399 7740 10424 10748 11430 2260
Size: 341 2684 324 682 5225
Fragments arranged by size:
5225 2684 1337 1235 896 876 682 341
324 290 279 114 112
PspXI vC'TCGA_Gb
Cuts at: 6087 6087
Size: 14395
PstI C_TGCA'G
Cuts at: 830 3772 4194 4583 5001 5232 9293 10876 11747
Size: 2942 422 389 418 231 4061 1583 871
Cuts at: 11747 830
Size: 3478
Fragments arranged by size:
4061 3478 2942 1583 871 422 418 389
231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14395
PvuII CAG'CTG
Cuts at: 2816 3031 3260 3776 4693 6023 7909 8929 10091
Size: 215 229 516 917 1330 1886 1020 1162
Cuts at: 10091 2816
Size: 7120
Fragments arranged by size:
7120 1886 1330 1162 1020 917 516 229
215
SacI G_AGCT'C
Cuts at: 5100 6011 8046 9000 9966 5100
Size: 911 2035 954 966 9529
Fragments arranged by size:
9529 2035 966 954 911
SalI G'TCGA_C
Cuts at: 1936 11737 1936
Size: 9801 4594
SanDI GG'GwC_CC
Cuts at: 4007 7130 11559 4007
Size: 3123 4429 6843
Fragments arranged by size:
6843 4429 3123
SapI GCTCTTCn'nnn_
Cuts at: 2270 2794 2812 2997 3111 3456 3675 9006 10023
Size: 524 18 185 114 345 219 5331 1017
Cuts at: 10023 2270
Size: 6642
Fragments arranged by size:
6642 5331 1017 524 345 219 185 114
18
SbfI CC_TGCA'GG
Cuts at: 11747 11747
Size: 14395
ScaI AGT'ACT
Cuts at: 2732 3662 12643 14355 2732
Size: 930 8981 1712 2772
Fragments arranged by size:
8981 2772 1712 930
SexAI A'CCwGG_T
Cuts at: 5892 6565 5892
Size: 673 13722
SfcI C'TryA_G
Cuts at: 826 1045 2048 3252 3768 4190 4579 4997 5228
Size: 219 1003 1204 516 422 389 418 231
Cuts at: 5228 6291 9289 10872 11474 11743 12878 13559 13750
Size: 1063 2998 1583 602 269 1135 681 191
Cuts at: 13750 826
Size: 1471
Fragments arranged by size:
2998 1583 1471 1204 1135 1063 1003 681
602 516 422 418 389 269 231 219
191
SfoI GGC'GCC
Cuts at: 5055 5055
Size: 14395
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14395
SgrAI Cr'CCGG_yG
Cuts at: 1700 9141 1700
Size: 7441 6954
SmaI CCC'GGG
Cuts at: 1872 8054 8480 8894 9212 10368 11722 1872
Size: 6182 426 414 318 1156 1354 4545
Fragments arranged by size:
6182 4545 1354 1156 426 414 318
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2160 2666 3315 4124 6087 6128
Size: 197 926 217 506 649 809 1963 41
Cuts at: 6128 7372 8107 8168 9222 9643 11102 12499 13400
Size: 1244 735 61 1054 421 1459 1397 901
Cuts at: 13400 13662 820
Size: 262 1553
Fragments arranged by size:
1963 1553 1459 1397 1244 1054 926 901
809 735 649 506 421 262 217 197
61 41
SnaBI TAC'GTA
Cuts at: 493 12186 493
Size: 11693 2702
SpeI A'CTAG_T
Cuts at: 152 10355 152
Size: 10203 4192
SphI G_CATG'C
Cuts at: 1132 6639 7979 11753 1132
Size: 5507 1340 3774 3774
Fragments arranged by size:
5507 3774 3774 1340
SrfI GCCC'GGGC
Cuts at: 9212 9212
Size: 14395
SspI AAT'ATT
Cuts at: 5 52 12319 5
Size: 47 12267 2081
Fragments arranged by size:
12267 2081 47
StuI AGG'CCT
Cuts at: 1517 4205 4507 6121 8303 8969 9331 9649 1517
Size: 2688 302 1614 2182 666 362 318 6263
Fragments arranged by size:
6263 2688 2182 1614 666 362 318 302
StyI C'CwwG_G
Cuts at: 513 1065 2595 2601 3182 3294 3333 3792 5907
Size: 552 1530 6 581 112 39 459 2115
Cuts at: 5907 6480 7419 7512 7536 7994 8248 8433 8784
Size: 573 939 93 24 458 254 185 351
Cuts at: 8784 9051 9326 9636 9834 11143 11256 11608 11834
Size: 267 275 310 198 1309 113 352 226
Cuts at: 11834 513
Size: 3074
Fragments arranged by size:
3074 2115 1530 1309 939 581 573 552
459 458 352 351 310 275 267 254
226 198 185 113 112 93 39 24
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6121 7084 8001 8108 8216 8792 9152
Size: 1255 4541 963 917 107 108 576 360
Cuts at: 9152 9838 10932 11026 12422 12439 12592 12777 13222
Size: 686 1094 94 1396 17 153 185 445
Cuts at: 13222 325
Size: 1498
Fragments arranged by size:
4541 1498 1396 1255 1094 963 917 686
576 445 360 185 153 108 107 94
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 3660 7879
Size: 274 80 33 51 1443 753 930 4219
Cuts at: 7879 12641 14353 96
Size: 4762 1712 138
Fragments arranged by size:
4762 4219 1712 1443 930 753 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2408 2440 2467 2499 5407 5439 6200 6232 6328
Size: 32 27 32 2908 32 761 32 96
Cuts at: 6328 6357 6360 6389 6438 6470 8353 8385 9493
Size: 29 3 29 49 32 1883 32 1108
Cuts at: 9493 9525 14355 14387 2408
Size: 32 4830 32 2416
Fragments arranged by size:
4830 2908 2416 1883 1108 761 96 49
32 32 32 32 32 32 32 32
29 29 27 3
Tth111I GACn'n_nGTC
Cuts at: 1316 7150 7198 7240 10685 1316
Size: 5834 48 42 3445 5026
Fragments arranged by size:
5834 5026 3445 48 42
XbaI T'CTAG_A
Cuts at: 11731 11731
Size: 14395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 5114 6073 6109 8959 9832 10183 2212
Size: 2902 959 36 2850 873 351 6424
Fragments arranged by size:
6424 2902 2850 959 873 351 36
XhoI C'TCGA_G
Cuts at: 1943 3315 6087 11102 1943
Size: 1372 2772 5015 5236
Fragments arranged by size:
5236 5015 2772 1372
XmaI C'CCGG_G
Cuts at: 1870 8052 8478 8892 9210 10366 11720 1870
Size: 6182 426 414 318 1156 1354 4545
Fragments arranged by size:
6182 4545 1354 1156 426 414 318
XmnI GAAnn'nnTTC
Cuts at: 2587 12524 2587
Size: 9937 4458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11573 12203 276
Size: 53 83 186 936 10039 630 2468
Fragments arranged by size:
10039 2468 936 630 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BfrBI BsiWI BstZ17I NsiI PacI PsrI RsrII
SacII SfiI SwaI