Restriction map for
pFN21AE9669
(Circular) (Six-base) MAPSORT of: pFN21AE9669.seq Check: 7247 from: 1 to: 13696
pFN21AE9669 13696 bp
With 182 enzymes: SgfI *
October 14, 2011 09:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5515 5515
Size: 13696
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5572 9602 11506 278
Size: 53 83 186 936 4036 4030 1904 2468
Fragments arranged by size:
4036 4030 2468 1904 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2379 11039 1937
Size: 442 8660 4594
Fragments arranged by size:
8660 4594 442
Acc65I G'GTAC_C
Cuts at: 2458 5994 11017 2458
Size: 3536 5023 5137
Fragments arranged by size:
5137 5023 3536
AclI AA'CG_TT
Cuts at: 1563 11823 12196 1563
Size: 10260 373 3063
Fragments arranged by size:
10260 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4236 4305 5354 5422 5460 5808 5930 6774
Size: 2604 69 1049 68 38 348 122 844
Cuts at: 6774 7217 7362 7614 8032 8871 9809 10262 10769
Size: 443 145 252 418 839 938 453 507
Cuts at: 10769 11759 13143 13411 1632
Size: 990 1384 268 1917
Fragments arranged by size:
2604 1917 1384 1049 990 938 844 839
507 453 443 418 348 268 252 145
122 69 68 38
AfeI AGC'GCT
Cuts at: 4277 4277
Size: 13696
AflII C'TTAA_G
Cuts at: 820 1017 8237 820
Size: 197 7220 6279
Fragments arranged by size:
7220 6279 197
AflIII A'CryG_T
Cuts at: 1203 7736 10428 12595 13572 1203
Size: 6533 2692 2167 977 1327
Fragments arranged by size:
6533 2692 2167 1327 977
AgeI A'CCGG_T
Cuts at: 12527 12527
Size: 13696
AhdI GACnn_n'nnGTC
Cuts at: 5274 7737 8686 8752 12425 5274
Size: 2463 949 66 3673 6545
Fragments arranged by size:
6545 3673 2463 949 66
AleI CACnn'nnGTG
Cuts at: 1166 3111 9062 1166
Size: 1945 5951 5800
Fragments arranged by size:
5951 5800 1945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5897 8198 1184
Size: 4713 2301 6682
Fragments arranged by size:
6682 4713 2301
AlwNI CAG_nnn'CTG
Cuts at: 2699 4334 5346 7067 8684 8786 9428 10592 11439
Size: 1635 1012 1721 1617 102 642 1164 847
Cuts at: 11439 13011 2699
Size: 1572 3384
Fragments arranged by size:
3384 1721 1635 1617 1572 1164 1012 847
642 102
ApaI G_GGCC'C
Cuts at: 2444 2535 3359 2444
Size: 91 824 12781
Fragments arranged by size:
12781 824 91
ApaLI G'TGCA_C
Cuts at: 6414 11753 12909 6414
Size: 5339 1156 7201
Fragments arranged by size:
7201 5339 1156
ApoI r'AATT_y
Cuts at: 1493 2995 4174 4478 4557 5105 5498 5677 5796
Size: 1502 1179 304 79 548 393 179 119
Cuts at: 5796 6256 6446 6589 7544 7613 7621 8803 9373
Size: 460 190 143 955 69 8 1182 570
Cuts at: 9373 10037 11005 11231 11383 13492 1493
Size: 664 968 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 1182 1179 968 955 664
570 548 460 393 304 226 190 179
152 143 119 79 69 8
AseI AT'TA_AT
Cuts at: 160 1032 12250 160
Size: 872 11218 1606
Fragments arranged by size:
11218 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13696
AvaI C'yCGr_G
Cuts at: 1870 1943 3565 7032 7836 8853 10702 11021 1870
Size: 73 1622 3467 804 1017 1849 319 4545
Fragments arranged by size:
4545 3467 1849 1622 1017 804 319 73
BamHI G'GATC_C
Cuts at: 3832 7320 9708 11026 11393 3832
Size: 3488 2388 1318 367 6135
Fragments arranged by size:
6135 3488 2388 1318 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2341 2458 2740 3326 3403
Size: 325 217 627 554 117 282 586 77
Cuts at: 3403 5994 9012 9943 9991 11017 12472 618
Size: 2591 3018 931 48 1026 1455 1842
Fragments arranged by size:
3018 2591 1842 1455 1026 931 627 586
554 325 282 217 117 77 48
BanII G_rGCy'C
Cuts at: 1391 2444 2479 2535 3359 3687 3999 7855 9102
Size: 1053 35 56 824 328 312 3856 1247
Cuts at: 9102 10802 11015 1391
Size: 1700 213 4072
Fragments arranged by size:
4072 3856 1700 1247 1053 824 328 312
213 56 35
BbeI G_GCGC'C
Cuts at: 9016 9995 9016
Size: 979 12717
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3576 4293 4752 5979 7569 7817 8757
Size: 971 1677 717 459 1227 1590 248 940
Cuts at: 8757 10521 10563 10926 928
Size: 1764 42 363 3698
Fragments arranged by size:
3698 1764 1677 1590 1227 971 940 717
459 363 248 42
BbvCI CC'TCA_GC
Cuts at: 2553 3303 9621 10830 2553
Size: 750 6318 1209 5419
Fragments arranged by size:
6318 5419 1209 750
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2918 2952 3359 3393 9227 9261 9453
Size: 34 981 34 407 34 5834 34 192
Cuts at: 9453 9487 11887 11921 1903
Size: 34 2400 34 3678
Fragments arranged by size:
5834 3678 2400 981 407 192 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3255 7948 11588 12798 3255
Size: 4693 3640 1210 4153
Fragments arranged by size:
4693 4153 3640 1210
BclI T'GATC_A
Cuts at: 1548 2212 6466 7005 1548
Size: 664 4254 539 8239
Fragments arranged by size:
8239 4254 664 539
BfrBI ATG'CAT
Cuts at: 2245 3148 2245
Size: 903 12793
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2658 10323 12307
Size: 107 122 71 631 605 986 7665 1984
Cuts at: 12307 136
Size: 1525
Fragments arranged by size:
7665 1984 1525 986 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4537 7281 13691 4537
Size: 2744 6410 4542
Fragments arranged by size:
6410 4542 2744
BlpI GC'TnA_GC
Cuts at: 3738 8895 9096 11113 3738
Size: 5157 201 2017 6321
Fragments arranged by size:
6321 5157 2017 201
Bme1580I G_kGCm'C
Cuts at: 1790 2346 2444 2535 2721 3359 3426 3619 6418
Size: 556 98 91 186 638 67 193 2799
Cuts at: 6418 11757 12913 1790
Size: 5339 1156 2573
Fragments arranged by size:
5339 2799 2573 1156 638 556 193 186
98 91 67
BmgBI CAC'GTC
Cuts at: 1318 7739 10218 1318
Size: 6421 2479 4796
Fragments arranged by size:
6421 4796 2479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 3429 3831
Size: 416 53 83 399 24 1688 324 402
Cuts at: 3831 4360 4397 5104 6308 8694 10128 10751 11427
Size: 529 37 707 1204 2386 1434 623 676
Cuts at: 11427 12385 442
Size: 958 1753
Fragments arranged by size:
2386 1753 1688 1434 1204 958 707 676
623 529 416 402 399 324 83 53
37 24
BmtI G_CTAG'C
Cuts at: 1056 7523 10670 1056
Size: 6467 3147 4082
Fragments arranged by size:
6467 4082 3147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6050 8173 8656 10966 6050
Size: 2123 483 2310 8780
Fragments arranged by size:
8780 2310 2123 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 4476 5399 5820 5834 6170 8451 8759
Size: 3074 163 923 421 14 336 2281 308
Cuts at: 8759 10035 10271 10280 10289 10317 10719 10786 12356
Size: 1276 236 9 9 28 402 67 1570
Cuts at: 12356 1239
Size: 2579
Fragments arranged by size:
3074 2579 2281 1570 1276 923 421 402
336 308 236 163 67 28 14 9
9
Bpu10I CC'TnA_GC
Cuts at: 2553 3303 5280 6192 6534 9621 10830 2553
Size: 750 1977 912 342 3087 1209 5419
Fragments arranged by size:
5419 3087 1977 1209 912 750 342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5351 5690 7285 9331 11821 12686 12984 5351
Size: 339 1595 2046 2490 865 298 6063
Fragments arranged by size:
6063 2490 2046 1595 865 339 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3456 7260 9307 9647 10850 12359 882
Size: 617 1957 3804 2047 340 1203 1509 2219
Fragments arranged by size:
3804 2219 2047 1957 1509 1203 617 340
BsaAI yAC'GTr
Cuts at: 493 1204 2588 2716 4679 4755 11487 493
Size: 711 1384 128 1963 76 6732 2702
Fragments arranged by size:
6732 2702 1963 1384 711 128 76
BsaBI GATnn'nnATC
Cuts at: 3064 6465 13690 3064
Size: 3401 7225 3070
Fragments arranged by size:
7225 3401 3070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5569 6781 9013 9599
Size: 53 83 186 936 4036 1212 2232 586
Cuts at: 9599 9992 11503 11885 12520 275
Size: 393 1511 382 635 1451
Fragments arranged by size:
4036 2232 1511 1451 1212 936 635 586
393 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4659 7052 10510 12129 12527 12801 12948 13359 4659
Size: 2393 3458 1619 398 274 147 411 4996
Fragments arranged by size:
4996 3458 2393 1619 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 2793 2823 3217
Size: 30 165 30 1011 30 349 30 394
Cuts at: 3217 3247 3532 3562 4449 4479 4689 4719 6044
Size: 30 285 30 887 30 210 30 1325
Cuts at: 6044 6074 6805 6835 8108 8138 9136 9166 1178
Size: 30 731 30 1273 30 998 30 5708
Fragments arranged by size:
5708 1325 1273 1011 998 887 731 394
349 285 210 165 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3219 3222 4197 4200 4328 4475 4808 6443
Size: 2033 3 975 3 128 147 333 1635
Cuts at: 6443 8113 8162 8454 8546 9100 9144 9220 9223
Size: 1670 49 292 92 554 44 76 3
Cuts at: 9223 9226 9229 9550 10550 10895 1186
Size: 3 3 321 1000 345 3987
Fragments arranged by size:
3987 2033 1670 1635 1000 975 554 345
333 321 292 147 128 92 76 49
44 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2721 2732 3257 4337 8220 8224 8545 8769 9279
Size: 11 525 1080 3883 4 321 224 510
Cuts at: 9279 10768 12899 13395 2721
Size: 1489 2131 496 3022
Fragments arranged by size:
3883 3022 2131 1489 1080 525 510 496
321 224 11 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2523 2783 6723 7145 9630 9779 1163
Size: 573 787 260 3940 422 2485 149 5080
Fragments arranged by size:
5080 3940 2485 787 573 422 260 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6019 10197 11146 11907 12935 1540
Size: 275 186 4018 4178 949 761 1028 2301
Fragments arranged by size:
4178 4018 2301 1028 949 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6418 7112 7855 8837 9102 11757 11842 12913 6418
Size: 694 743 982 265 2655 85 1071 7201
Fragments arranged by size:
7201 2655 1071 982 743 694 265 85
BsmI GAATG_Cn'
Cuts at: 3670 3739 8091 9844 10795 11207 11300 3670
Size: 69 4352 1753 951 412 93 6066
Fragments arranged by size:
6066 4352 1753 951 412 93 69
BsmBI CGTCTCn'nnnn_
Cuts at: 2307 4240 5560 5576 9156 2307
Size: 1933 1320 16 3580 6847
Fragments arranged by size:
6847 3580 1933 1320 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2444 2479 2535 2721 3359 3426
Size: 399 556 98 35 56 186 638 67
Cuts at: 3426 3619 3687 3999 6418 7112 7855 8837 9102
Size: 193 68 312 2419 694 743 982 265
Cuts at: 9102 10802 11015 11757 11842 12913 1391
Size: 1700 213 742 85 1071 2174
Fragments arranged by size:
2419 2174 1700 1071 982 743 742 694
638 556 399 312 265 213 193 186
98 85 68 67 56 35
BspEI T'CCGG_A
Cuts at: 13359 13359
Size: 13696
BspHI T'CATG_A
Cuts at: 8905 9582 11585 13315 8905
Size: 677 2003 1730 9286
Fragments arranged by size:
9286 2003 1730 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3771 5515 6152 8194 8405 11051 844
Size: 2927 1744 637 2042 211 2646 3489
Fragments arranged by size:
3489 2927 2646 2042 1744 637 211
BsrBI CCG'CTC
Cuts at: 1394 1823 4762 11583 1394
Size: 429 2939 6821 3507
Fragments arranged by size:
6821 3507 2939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3213 5862 8827 12191 12365 65
Size: 1183 1965 2649 2965 3364 174 1396
Fragments arranged by size:
3364 2965 2649 1965 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2518 7117 7746 7818 12340 12527 1700
Size: 110 708 4599 629 72 4522 187 2869
Fragments arranged by size:
4599 4522 2869 708 629 187 110 72
BsrGI T'GTAC_A
Cuts at: 96 6098 96
Size: 6002 7694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13696
BssSI C'ACGA_G
Cuts at: 11756 12768 11756
Size: 1012 12684
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3655 4064 4325 4334 9617 10349 794
Size: 2861 409 261 9 5283 732 4141
Fragments arranged by size:
5283 4141 2861 732 409 261 9
BstBI TT'CG_AA
Cuts at: 12500 12500
Size: 13696
BstEII G'GTnAC_C
Cuts at: 1184 2087 9625 1184
Size: 903 7538 5255
Fragments arranged by size:
7538 5255 903
BstXI CCAn_nnnn'nTGG
Cuts at: 3113 3323 5870 6594 8274 9064 10021 3113
Size: 210 2547 724 1680 790 957 6788
Fragments arranged by size:
6788 2547 1680 957 790 724 210
BstYI r'GATC_y
Cuts at: 1972 2011 3832 4537 5197 6388 6471 7000 7281
Size: 39 1821 705 660 1191 83 529 281
Cuts at: 7281 7320 8193 8260 8881 9708 10006 10619 11026
Size: 39 873 67 621 827 298 613 407
Cuts at: 11026 11393 11778 11795 13247 13333 13345 13691 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1821 1452 1191 873 827 705 660
621 613 529 407 385 367 346 298
281 86 83 67 39 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2337 2692 3162 8937 2337
Size: 355 470 5775 7096
Fragments arranged by size:
7096 5775 470 355
BtgI C'CryG_G
Cuts at: 513 1065 2004 2347 2797 3330 5050 5297 6776
Size: 552 939 343 450 533 1720 247 1479
Cuts at: 6776 6883 7650 9105 10191 10794 513
Size: 107 767 1455 1086 603 3415
Fragments arranged by size:
3415 1720 1479 1455 1086 939 767 603
552 533 450 343 247 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 10380 10887 487
Size: 683 56 9154 507 3296
Fragments arranged by size:
9154 3296 683 507 56
BtsI GCAGTG_nn'
Cuts at: 2362 3551 6129 6711 8835 8988 11212 12006 12026
Size: 1189 2578 582 2124 153 2224 794 20
Cuts at: 12026 2362
Size: 4032
Fragments arranged by size:
4032 2578 2224 2124 1189 794 582 153
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13696
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5683 5718 529
Size: 35 5119 35 8507
Fragments arranged by size:
8507 5119 35 35
DraI TTT'AAA
Cuts at: 5812 10846 11000 5812
Size: 5034 154 8508
Fragments arranged by size:
8508 5034 154
DraIII CAC_nnn'GTG
Cuts at: 2761 5392 5929 10937 2761
Size: 2631 537 5008 5520
Fragments arranged by size:
5520 5008 2631 537
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9891 10903 12703 809
Size: 507 8575 1012 1800 1802
Fragments arranged by size:
8575 1802 1800 1012 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2701 3496 5920
Size: 54 1425 50 275 18 871 795 2424
Cuts at: 5920 6016 7024 7143 8061 8999 9112 10194 11143
Size: 96 1008 119 918 938 113 1082 949
Cuts at: 11143 12032 13661 8
Size: 889 1629 43
Fragments arranged by size:
2424 1629 1425 1082 1008 949 938 918
889 871 795 275 119 113 96 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6016 10194 11143 1537
Size: 275 4204 4178 949 4090
Fragments arranged by size:
4204 4178 4090 949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3743 4238 4262 5441 5514 5784 5799 6331
Size: 2390 495 24 1179 73 270 15 532
Cuts at: 6331 7903 7908 8571 8712 8721 8754 9077 9527
Size: 1572 5 663 141 9 33 323 450
Cuts at: 9527 10147 10715 10935 11626 1353
Size: 620 568 220 691 3423
Fragments arranged by size:
3423 2390 1572 1179 691 663 620 568
532 495 450 323 270 220 141 73
33 24 15 9 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6026 7289 12283 12657 12803 387
Size: 1064 138 4437 1263 4994 374 146 1280
Fragments arranged by size:
4994 4437 1280 1263 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 7853 9100 7853
Size: 1247 12449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4236 4305 4313 4476 5354 5399 5422
Size: 393 2604 69 8 163 878 45 23
Cuts at: 5422 5460 5808 5820 5834 5930 6170 6774 7217
Size: 38 348 12 14 96 240 604 443
Cuts at: 7217 7362 7614 8032 8451 8759 8871 9809 10035
Size: 145 252 418 419 308 112 938 226
Cuts at: 10035 10262 10271 10280 10289 10317 10719 10769 10786
Size: 227 9 9 9 28 402 50 17
Cuts at: 10786 11759 12356 13143 13411 1239
Size: 973 597 787 268 1524
Fragments arranged by size:
2604 1524 973 938 878 787 604 597
443 419 418 402 393 348 308 268
252 240 227 226 163 145 112 96
69 50 45 38 28 23 17 14
12 9 9 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2666 2960 6038 6265 7169 8005 2666
Size: 294 3078 227 904 836 8357
Fragments arranged by size:
8357 3078 904 836 294 227
EcoO109I rG'GnC_Cy
Cuts at: 2440 2441 2531 2532 2659 2696 3356 3912 4228
Size: 1 90 1 127 37 660 556 316
Cuts at: 4228 5223 6405 7216 7801 10838 2440
Size: 995 1182 811 585 3037 5298
Fragments arranged by size:
5298 3037 1182 995 811 660 585 556
316 127 90 37 1 1
EcoRI G'AATT_C
Cuts at: 4478 4557 5105 11005 11383 4478
Size: 79 548 5900 378 6791
Fragments arranged by size:
6791 5900 548 378 79
EcoRV GAT'ATC
Cuts at: 2942 2942
Size: 13696
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 8841 9106 9418 9485 8841
Size: 265 312 67 13052
Fragments arranged by size:
13052 312 265 67
FspI TGC'GCA
Cuts at: 12202 12202
Size: 13696
HaeII r_GCGC'y
Cuts at: 1678 4279 5305 9016 9995 12843 1678
Size: 2601 1026 3711 979 2848 2531
Fragments arranged by size:
3711 2848 2601 2531 1026 979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 4337 4369 4699
Size: 32 135 32 842 32 1891 32 330
Cuts at: 4699 4731 5260 5292 6044 6076 6425 6457 7026
Size: 32 529 32 752 32 349 32 569
Cuts at: 7026 7058 7723 7755 8006 8038 8108 8140 8329
Size: 32 665 32 251 32 70 32 189
Cuts at: 8329 8361 8672 8704 8738 8770 9244 9276 12337
Size: 32 311 32 34 32 474 32 3061
Cuts at: 12337 12369 12411 12443 1373
Size: 32 42 32 2626
Fragments arranged by size:
3061 2626 1891 842 752 665 569 529
474 349 330 311 251 189 135 70
42 34 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2974 3515 4147 4657 7045 11040
Size: 988 281 1036 541 632 510 2388 3995
Cuts at: 11040 11286 669
Size: 246 3079
Fragments arranged by size:
3995 3079 2388 1036 988 632 541 510
281 246
HindIII A'AGCT_T
Cuts at: 748 6988 8254 9633 748
Size: 6240 1266 1379 4811
Fragments arranged by size:
6240 4811 1379 1266
HpaI GTT'AAC
Cuts at: 2974 11286 2974
Size: 8312 5384
KasI G'GCGC_C
Cuts at: 9012 9991 9012
Size: 979 12717
KpnI G_GTAC'C
Cuts at: 2462 5998 11021 2462
Size: 3536 5023 5137
Fragments arranged by size:
5137 5023 3536
MfeI C'AATT_G
Cuts at: 4948 11295 4948
Size: 6347 7349
MluI A'CGCG_T
Cuts at: 13572 13572
Size: 13696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3660 4380 4902 5144 5337 8315 8428 8736
Size: 2355 720 522 242 193 2978 113 308
Cuts at: 8736 12810 12994 1305
Size: 4074 184 2007
Fragments arranged by size:
4074 2978 2355 2007 720 522 308 242
193 184 113
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2703 3498 5922 7026 7145
Size: 54 1425 343 871 795 2424 1104 119
Cuts at: 7145 8063 9001 10
Size: 918 938 4705
Fragments arranged by size:
4705 2424 1425 1104 938 918 871 795
343 119 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3111 5489 5738 6944 7410 9062 9269
Size: 648 1945 2378 249 1206 466 1652 207
Cuts at: 9269 11654 12013 12172 518
Size: 2385 359 159 2042
Fragments arranged by size:
2385 2378 2042 1945 1652 1206 648 466
359 249 207 159
MspA1I CmG'CkG
Cuts at: 1606 7019 7149 8224 8549 8601 9202 9691 10193
Size: 5413 130 1075 325 52 601 489 502
Cuts at: 10193 10202 10309 10349 10469 11112 11789 12937 13182
Size: 9 107 40 120 643 677 1148 245
Cuts at: 13182 1606
Size: 2120
Fragments arranged by size:
5413 2120 1148 1075 677 643 601 502
489 325 245 130 120 107 52 40
9
NaeI GCC'GGC
Cuts at: 2520 7748 2520
Size: 5228 8468
NarI GG'CG_CC
Cuts at: 9013 9992 9013
Size: 979 12717
NcoI C'CATG_G
Cuts at: 513 1065 2004 2347 2797 3330 5050 5297 7650
Size: 552 939 343 450 533 1720 247 2353
Cuts at: 7650 9105 513
Size: 1455 5104
Fragments arranged by size:
5104 2353 1720 1455 939 552 533 450
343 247
NdeI CA'TA_TG
Cuts at: 387 6001 387
Size: 5614 8082
NgoMIV G'CCGG_C
Cuts at: 2518 7746 2518
Size: 5228 8468
NheI G'CTAG_C
Cuts at: 1052 7519 10666 1052
Size: 6467 3147 4082
Fragments arranged by size:
6467 4082 3147
NotI GC'GGCC_GC
Cuts at: 10194 11143 10194
Size: 949 12747
NsiI A_TGCA'T
Cuts at: 2247 3150 2247
Size: 903 12793
NspI r_CATG'y
Cuts at: 1132 1229 2392 2563 3370 3397 4963 7237 7519
Size: 97 1163 171 807 27 1566 2274 282
Cuts at: 7519 7664 11054 12599 1132
Size: 145 3390 1545 2229
Fragments arranged by size:
3390 2274 2229 1566 1545 1163 807 282
171 145 97 27
PasI CC'CwG_GG
Cuts at: 1348 2285 2445 5203 1348
Size: 937 160 2758 9841
Fragments arranged by size:
9841 2758 937 160
PciI A'CATG_T
Cuts at: 12595 12595
Size: 13696
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 13696
PfoI T'CCnGG_A
Cuts at: 2014 3477 7170 9682 10333 10735 2014
Size: 1463 3693 2512 651 402 4975
Fragments arranged by size:
4975 3693 2512 1463 651 402
PmeI GTTT'AAAC
Cuts at: 11000 11000
Size: 13696
PmlI CAC'GTG
Cuts at: 2588 2716 4755 2588
Size: 128 2039 11529
Fragments arranged by size:
11529 2039 128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4414 4446 10556 10588 11765 11797 13278 13310 4414
Size: 32 6110 32 1177 32 1481 32 4800
Fragments arranged by size:
6110 4800 1481 1177 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2696 3912 4228 5223 7216 10838 2696
Size: 1216 316 995 1993 3622 5554
Fragments arranged by size:
5554 3622 1993 1216 995 316
PshAI GACnn'nnGTC
Cuts at: 1613 2299 1613
Size: 686 13010
PsiI TTA'TAA
Cuts at: 11266 11266
Size: 13696
PspOMI G'GGCC_C
Cuts at: 2440 2531 3355 2440
Size: 91 824 12781
Fragments arranged by size:
12781 824 91
PspXI vC'TCGA_Gb
Cuts at: 7032 7032
Size: 13696
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3474 3506 3474
Size: 32 13664
PstI C_TGCA'G
Cuts at: 830 3558 6935 7354 10903 11048 830
Size: 2728 3377 419 3549 145 3478
Fragments arranged by size:
3549 3478 3377 2728 419 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13696
PvuII CAG'CTG
Cuts at: 7149 8224 8549 9691 10309 10349 10469 7149
Size: 1075 325 1142 618 40 120 10376
Fragments arranged by size:
10376 1142 1075 618 325 120 40
SacI G_AGCT'C
Cuts at: 7855 9102 7855
Size: 1247 12449
SacII CC_GC'GG
Cuts at: 10194 10194
Size: 13696
SalI G'TCGA_C
Cuts at: 1936 11038 1936
Size: 9102 4594
SanDI GG'GwC_CC
Cuts at: 3912 3912
Size: 13696
SapI GCTCTTCn'nnn_
Cuts at: 5514 10935 5514
Size: 5421 8275
SbfI CC_TGCA'GG
Cuts at: 11048 11048
Size: 13696
ScaI AGT'ACT
Cuts at: 11944 13656 11944
Size: 1712 11984
SexAI A'CCwGG_T
Cuts at: 6654 6654
Size: 13696
SfcI C'TryA_G
Cuts at: 826 1045 3054 3554 4530 6238 6320 6697 6931
Size: 219 2009 500 976 1708 82 377 234
Cuts at: 6931 7350 10125 10899 11044 12179 12860 13051 826
Size: 419 2775 774 145 1135 681 191 1471
Fragments arranged by size:
2775 2009 1708 1471 1135 976 774 681
500 419 377 234 219 191 145 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 2658 2658
Size: 13696
SfoI GGC'GCC
Cuts at: 9014 9993 9014
Size: 979 12717
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13696
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13696
SmaI CCC'GGG
Cuts at: 1872 3567 10704 11023 1872
Size: 1695 7137 319 4545
Fragments arranged by size:
7137 4545 1695 319
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5366 5669 7032 7300 7836 8237
Size: 197 926 3423 303 1363 268 536 401
Cuts at: 8237 9346 11800 12701 12963 820
Size: 1109 2454 901 262 1553
Fragments arranged by size:
3423 2454 1553 1363 1109 926 901 536
401 303 268 262 197
SnaBI TAC'GTA
Cuts at: 493 11487 493
Size: 10994 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13696
SphI G_CATG'C
Cuts at: 1132 2392 7237 11054 1132
Size: 1260 4845 3817 3774
Fragments arranged by size:
4845 3817 3774 1260
SspI AAT'ATT
Cuts at: 5 52 2050 5293 5654 11620 5
Size: 47 1998 3243 361 5966 2081
Fragments arranged by size:
5966 3243 2081 1998 361 47
StuI AGG'CCT
Cuts at: 1517 2073 3003 9036 9344 9468 10317 1517
Size: 556 930 6033 308 124 849 4896
Fragments arranged by size:
6033 4896 930 849 556 308 124
StyI C'CwwG_G
Cuts at: 513 1065 2004 2137 2347 2647 2797 3330 3407
Size: 552 939 133 210 300 150 533 77
Cuts at: 3407 3457 3611 3981 3999 5050 5297 6576 7038
Size: 50 154 370 18 1051 247 1279 462
Cuts at: 7038 7073 7482 7650 8553 9031 9105 11135 513
Size: 35 409 168 903 478 74 2030 3074
Fragments arranged by size:
3074 2030 1279 1051 939 903 552 533
478 462 409 370 300 247 210 168
154 150 133 77 74 50 35 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2363 3211 3642 3745 7027 8310 9070
Size: 1255 783 848 431 103 3282 1283 760
Cuts at: 9070 9496 9968 11723 11740 11893 12078 12523 325
Size: 426 472 1755 17 153 185 445 1498
Fragments arranged by size:
3282 1755 1498 1283 1255 848 783 760
472 445 431 426 185 153 103 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5187 6098 6948
Size: 274 80 33 51 1443 3210 911 850
Cuts at: 6948 7239 7419 11942 13654 96
Size: 291 180 4523 1712 138
Fragments arranged by size:
4523 3210 1712 1443 911 850 291 274
180 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3217 3249 3459 3491 4689 4721 7080 7112 9136
Size: 32 210 32 1198 32 2359 32 2024
Cuts at: 9136 9168 13656 13688 3217
Size: 32 4488 32 3225
Fragments arranged by size:
4488 3225 2359 2024 1198 210 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 13696
XbaI T'CTAG_A
Cuts at: 3691 8792 11032 3691
Size: 5101 2240 6355
Fragments arranged by size:
6355 5101 2240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2354 3506 2354
Size: 1152 12544
XhoI C'TCGA_G
Cuts at: 1943 7032 7836 1943
Size: 5089 804 7803
Fragments arranged by size:
7803 5089 804
XmaI C'CCGG_G
Cuts at: 1870 3565 10702 11021 1870
Size: 1695 7137 319 4545
Fragments arranged by size:
7137 4545 1695 319
XmnI GAAnn'nnTTC
Cuts at: 2051 4604 5678 8045 11825 2051
Size: 2553 1074 2367 3780 3922
Fragments arranged by size:
3922 3780 2553 2367 1074
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5570 9600 11504 276
Size: 53 83 186 936 4036 4030 1904 2468
Fragments arranged by size:
4036 4030 2468 1904 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BstZ17I FseI FspAI
NruI PacI RsrII SrfI SwaI