Restriction map for
pFN21AE9668
(Circular) (Six-base) MAPSORT of: pFN21AE9668.seq Check: 1609 from: 1 to: 8791
pFN21AE9668 8791 bp
With 182 enzymes: SgfI *
December 21, 2016 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4500 4527 4768 5448 4500
Size: 27 241 680 7843
Fragments arranged by size:
7843 680 241 27
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3270 6601 278
Size: 53 83 186 936 1734 3331 2468
Fragments arranged by size:
3331 2468 1734 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2084 6134 1937
Size: 147 4050 4594
Fragments arranged by size:
4594 4050 147
Acc65I G'GTAC_C
Cuts at: 2590 6112 2590
Size: 3522 5269
AclI AA'CG_TT
Cuts at: 1563 6918 7291 1563
Size: 5355 373 3063
Fragments arranged by size:
5355 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3956 4001 5362 5747 6854 8238 8506 1632
Size: 2324 45 1361 385 1107 1384 268 1917
Fragments arranged by size:
2324 1917 1384 1361 1107 385 268 45
AfeI AGC'GCT
Cuts at: 2342 4010 2342
Size: 1668 7123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8594
AflIII A'CryG_T
Cuts at: 1203 7690 8667 1203
Size: 6487 977 1327
Fragments arranged by size:
6487 1327 977
AgeI A'CCGG_T
Cuts at: 5171 7622 5171
Size: 2451 6340
AhdI GACnn_n'nnGTC
Cuts at: 7520 7520
Size: 8791
AleI CACnn'nnGTG
Cuts at: 1166 4415 1166
Size: 3249 5542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5925 1184
Size: 4741 4050
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5588 5620 5588
Size: 32 8759
AlwNI CAG_nnn'CTG
Cuts at: 2743 3535 4807 4912 5239 5290 6534 8106 2743
Size: 792 1272 105 327 51 1244 1572 3428
Fragments arranged by size:
3428 1572 1272 1244 792 327 105 51
ApaI G_GGCC'C
Cuts at: 5527 5830 5527
Size: 303 8488
ApaLI G'TGCA_C
Cuts at: 3566 3905 4994 6848 8004 3566
Size: 339 1089 1854 1156 4353
Fragments arranged by size:
4353 1854 1156 1089 339
ApoI r'AATT_y
Cuts at: 1493 3940 6046 6100 6326 6478 8587 1493
Size: 2447 2106 54 226 152 2109 1697
Fragments arranged by size:
2447 2109 2106 1697 226 152 54
AseI AT'TA_AT
Cuts at: 160 1032 7345 160
Size: 872 6313 1606
Fragments arranged by size:
6313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8791
AvaI C'yCGr_G
Cuts at: 1870 1943 4196 5697 6116 1870
Size: 73 2253 1501 419 4545
Fragments arranged by size:
4545 2253 1501 419 73
BamHI G'GATC_C
Cuts at: 6121 6488 6121
Size: 367 8424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2590 2728 4191 4417 5145
Size: 325 217 627 803 138 1463 226 728
Cuts at: 5145 5847 6112 7567 618
Size: 702 265 1455 1842
Fragments arranged by size:
1842 1463 1455 803 728 702 627 325
265 226 217 138
BanII G_rGCy'C
Cuts at: 1391 2943 3529 3546 3930 4121 4434 5376 5403
Size: 1552 586 17 384 191 313 942 27
Cuts at: 5403 5527 5606 5830 5948 6110 1391
Size: 124 79 224 118 162 4072
Fragments arranged by size:
4072 1552 942 586 384 313 224 191
162 124 118 79 27 17
BbeI G_GCGC'C
Cuts at: 5851 5851
Size: 8791
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2858 4155 5564 6016 928
Size: 971 959 1297 1409 452 3703
Fragments arranged by size:
3703 1409 1297 971 959 452
BbvCI CC'TCA_GC
Cuts at: 2637 5112 2637
Size: 2475 6316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3560 3594 6982 7016 1903
Size: 34 1623 34 3388 34 3678
Fragments arranged by size:
3678 3388 1623 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2390 6683 7893 2390
Size: 4293 1210 3288
Fragments arranged by size:
4293 3288 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2176 4618 4660
Size: 107 122 71 631 605 504 2442 42
Cuts at: 4660 5449 5729 5825 7402 136
Size: 789 280 96 1577 1525
Fragments arranged by size:
2442 1577 1525 789 631 605 504 280
122 107 96 71 42
BglII A'GATC_T
Cuts at: 8786 8786
Size: 8791
BlpI GC'TnA_GC
Cuts at: 3540 3857 6208 3540
Size: 317 2351 6123
Fragments arranged by size:
6123 2351 317
Bme1580I G_kGCm'C
Cuts at: 1790 2766 3012 3426 3570 3909 4194 4266 4422
Size: 976 246 414 144 339 285 72 156
Cuts at: 4422 4998 5527 5830 6852 8008 1790
Size: 576 529 303 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 976 576 529 414 339
303 285 246 156 144 72
BmgBI CAC'GTC
Cuts at: 1318 2546 3007 3148 5843 1318
Size: 1228 461 141 2695 4266
Fragments arranged by size:
4266 2695 1228 461 141
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3167 5950 6522
Size: 416 53 83 399 24 1750 2783 572
Cuts at: 6522 7480 442
Size: 958 1753
Fragments arranged by size:
2783 1753 1750 958 572 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2231 2941 4150 2231
Size: 710 1209 6872
Fragments arranged by size:
6872 1209 710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2451 2775 3245 3326 3530 4025 4105 4448
Size: 1212 324 470 81 204 495 80 343
Cuts at: 4448 4566 4763 5547 5831 7451 1239
Size: 118 197 784 284 1620 2579
Fragments arranged by size:
2579 1620 1212 784 495 470 343 324
284 204 197 118 81 80
Bpu10I CC'TnA_GC
Cuts at: 2370 2637 3249 4503 4533 5112 5733 2370
Size: 267 612 1254 30 579 621 5428
Fragments arranged by size:
5428 1254 621 612 579 267 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2589 3050 3071 4032 6916 7781 8079 2201
Size: 388 461 21 961 2884 865 298 2913
Fragments arranged by size:
2913 2884 961 865 461 388 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 3058 3968 4579 7454 882
Size: 617 645 914 910 611 2875 2219
Fragments arranged by size:
2875 2219 914 910 645 617 611
BsaAI yAC'GTr
Cuts at: 493 1204 3798 6582 493
Size: 711 2594 2784 2702
Fragments arranged by size:
2784 2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 3962 8785 3962
Size: 4823 3968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2750 3267 5848 6013
Size: 53 83 186 936 1217 517 2581 165
Cuts at: 6013 6598 6980 7615 275
Size: 585 382 635 1451
Fragments arranged by size:
2581 1451 1217 936 635 585 517 382
186 165 83 53
BsaWI w'CCGG_w
Cuts at: 5171 7224 7622 7896 8043 8454 5171
Size: 2053 398 274 147 411 5508
Fragments arranged by size:
5508 2053 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4686 4716 5588 5618 1178
Size: 30 165 30 3283 30 872 30 4351
Fragments arranged by size:
4351 3283 872 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2607 2640 3404 3472 3530 3533 3699 3857
Size: 1421 33 764 68 58 3 166 158
Cuts at: 3857 4069 4126 4569 5354 5635 5723 5800 5849
Size: 212 57 443 785 281 88 77 49
Cuts at: 5849 1186
Size: 4128
Fragments arranged by size:
4128 1421 785 764 443 281 212 166
158 88 77 68 58 57 49 33
3
BseYI C'CCAG_C
Cuts at: 2109 2736 2769 2799 2956 3028 3528 3597 3892
Size: 627 33 30 157 72 500 69 295
Cuts at: 3892 4266 4287 4434 4476 4524 4605 4622 4666
Size: 374 21 147 42 48 81 17 44
Cuts at: 4666 4860 4944 4950 4962 5259 5316 5445 5949
Size: 194 84 6 12 297 57 129 504
Cuts at: 5949 7994 8490 2109
Size: 2045 496 2410
Fragments arranged by size:
2410 2045 627 504 500 496 374 297
295 194 157 147 129 84 81 72
69 57 48 44 42 33 30 21
17 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2678 4472 4838 4874 4922 5642 1163
Size: 573 942 1794 366 36 48 720 4312
Fragments arranged by size:
4312 1794 942 720 573 366 48 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5396 5453 6241 7002 8030 1540
Size: 275 186 3395 57 788 761 1028 2301
Fragments arranged by size:
3395 2301 1028 788 761 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2217 3209 3546 3570 3909 4121 4539 4998 5745
Size: 992 337 24 339 212 418 459 747
Cuts at: 5745 5948 6852 6937 8008 2217
Size: 203 904 85 1071 3000
Fragments arranged by size:
3000 1071 992 904 747 459 418 339
337 212 203 85 24
BsmI GAATG_Cn'
Cuts at: 2371 6302 6395 2371
Size: 3931 93 4767
Fragments arranged by size:
4767 3931 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2766 2943 3012 3209 3426 3529
Size: 399 427 549 177 69 197 217 103
Cuts at: 3529 3546 3570 3909 3930 4121 4194 4266 4422
Size: 17 24 339 21 191 73 72 156
Cuts at: 4422 4434 4539 4998 5376 5403 5527 5606 5745
Size: 12 105 459 378 27 124 79 139
Cuts at: 5745 5830 5948 6110 6852 6937 8008 1391
Size: 85 118 162 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 549 459 427 399 378
339 217 197 191 177 162 156 139
124 118 105 103 85 85 79 73
72 69 27 24 21 17 12
BspEI T'CCGG_A
Cuts at: 8454 8454
Size: 8791
BspHI T'CATG_A
Cuts at: 2517 2718 6680 8410 2517
Size: 201 3962 1730 2898
Fragments arranged by size:
3962 2898 1730 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 4500 4527 4768 4806 5448 6146 844
Size: 1364 2292 27 241 38 642 698 3489
Fragments arranged by size:
3489 2292 1364 698 642 241 38 27
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 2789 5696 5764 6678 1394
Size: 429 196 770 2907 68 914 3507
Fragments arranged by size:
3507 2907 914 770 429 196 68
BsrDI GCAATG_nn'
Cuts at: 65 1248 3754 4404 7286 7460 65
Size: 1183 2506 650 2882 174 1396
Fragments arranged by size:
2882 2506 1396 1183 650 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2979 4073 5171 7435 7622 1700
Size: 110 1169 1094 1098 2264 187 2869
Fragments arranged by size:
2869 2264 1169 1098 1094 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8791
BssSI C'ACGA_G
Cuts at: 2915 3569 5324 6851 7863 2915
Size: 654 1755 1527 1012 3843
Fragments arranged by size:
3843 1755 1527 1012 654
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2200 4807 4816 4912 5008 794
Size: 1406 2607 9 96 96 4577
Fragments arranged by size:
4577 2607 1406 96 96 9
BstBI TT'CG_AA
Cuts at: 3354 7595 3354
Size: 4241 4550
BstEII G'GTnAC_C
Cuts at: 1184 5174 1184
Size: 3990 4801
BstXI CCAn_nnnn'nTGG
Cuts at: 2956 3442 2956
Size: 486 8305
BstYI r'GATC_y
Cuts at: 1972 5346 6121 6488 6873 6890 8342 8428 8440
Size: 3374 775 367 385 17 1452 86 12
Cuts at: 8440 8786 1972
Size: 346 1977
Fragments arranged by size:
3374 1977 1452 775 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3387 3387
Size: 8791
BtgI C'CryG_G
Cuts at: 513 1065 2586 3195 3327 4716 4985 5304 6024
Size: 552 1521 609 132 1389 269 319 720
Cuts at: 6024 513
Size: 3280
Fragments arranged by size:
3280 1521 1389 720 609 552 319 269
132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2812 4394 487
Size: 683 56 1586 1582 4884
Fragments arranged by size:
4884 1586 1582 683 56
BtsI GCAGTG_nn'
Cuts at: 2741 3426 3992 4575 6307 7101 7121 2741
Size: 685 566 583 1732 794 20 4411
Fragments arranged by size:
4411 1732 794 685 583 566 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2884 2919 3421 3456 529
Size: 35 2320 35 502 35 5864
Fragments arranged by size:
5864 2320 502 35 35 35
DraI TTT'AAA
Cuts at: 6095 6095
Size: 8791
DraIII CAC_nnn'GTG
Cuts at: 3998 4570 3998
Size: 572 8219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2702 5248 7798 809
Size: 507 1386 2546 2550 1802
Fragments arranged by size:
2550 2546 1802 1386 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2832 3324 3402
Size: 54 1425 50 275 18 1002 492 78
Cuts at: 3402 4465 5393 5450 6238 7127 8756 8
Size: 1063 928 57 788 889 1629 43
Fragments arranged by size:
1629 1425 1063 1002 928 889 788 492
275 78 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5393 5450 6238 1537
Size: 275 3581 57 788 4090
Fragments arranged by size:
4090 3581 788 275 57
EarI CTCTTCn'nnn_
Cuts at: 1353 2477 2533 2864 3035 3672 3773 3807 4746
Size: 1124 56 331 171 637 101 34 939
Cuts at: 4746 6721 1353
Size: 1975 3423
Fragments arranged by size:
3423 1975 1124 939 637 331 171 101
56 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5058 5675 7378 7752 7898 387
Size: 1064 138 3469 617 1703 374 146 1280
Fragments arranged by size:
3469 1703 1280 1064 617 374 146 138
EcoICRI GAG'CTC
Cuts at: 3544 4119 5946 3544
Size: 575 1827 6389
Fragments arranged by size:
6389 1827 575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2775 3245 3326 3530 3956 4001
Size: 393 819 324 470 81 204 426 45
Cuts at: 4001 4025 4105 4448 4566 4763 5362 5547 5747
Size: 24 80 343 118 197 599 185 200
Cuts at: 5747 5831 6854 7451 8238 8506 1239
Size: 84 1023 597 787 268 1524
Fragments arranged by size:
1524 1023 819 787 599 597 470 426
393 343 324 268 204 200 197 185
118 84 81 80 45 24
EcoO109I rG'GnC_Cy
Cuts at: 4249 5428 5523 5524 5581 5722 4249
Size: 1179 95 1 57 141 7318
Fragments arranged by size:
7318 1179 141 95 57 1
EcoRI G'AATT_C
Cuts at: 3940 6100 6478 3940
Size: 2160 378 6253
Fragments arranged by size:
6253 2160 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 2491 6101 2359
Size: 132 3610 5049
Fragments arranged by size:
5049 3610 132
FspI TGC'GCA
Cuts at: 7297 7297
Size: 8791
HaeII r_GCGC'y
Cuts at: 1678 2344 3773 4012 5851 5875 7938 1678
Size: 666 1429 239 1839 24 2063 2531
Fragments arranged by size:
2531 2063 1839 1429 666 239 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3467 3499 5150 5182 6050
Size: 32 135 32 1895 32 1651 32 868
Cuts at: 6050 6082 7432 7464 7506 7538 1373
Size: 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1895 1651 1350 868 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6135 6381 669
Size: 988 281 4197 246 3079
Fragments arranged by size:
4197 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8791
HpaI GTT'AAC
Cuts at: 6381 6381
Size: 8791
KasI G'GCGC_C
Cuts at: 5847 5847
Size: 8791
KpnI G_GTAC'C
Cuts at: 2594 6116 2594
Size: 3522 5269
MfeI C'AATT_G
Cuts at: 6390 6390
Size: 8791
MluI A'CGCG_T
Cuts at: 8667 8667
Size: 8791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 3309 3786 3969 4092 5053 5136 5657
Size: 876 1128 477 183 123 961 83 521
Cuts at: 5657 7905 8089 1305
Size: 2248 184 2007
Fragments arranged by size:
2248 2007 1128 961 876 521 477 184
183 123 83
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3326 10
Size: 54 1425 343 1494 5475
Fragments arranged by size:
5475 1494 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2723 3200 4415 4778 4784 6041 6749
Size: 648 1557 477 1215 363 6 1257 708
Cuts at: 6749 7108 7267 518
Size: 359 159 2042
Fragments arranged by size:
2042 1557 1257 1215 708 648 477 363
359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2965 3078 3532 4637 4792 4807 4912 5449
Size: 1359 113 454 1105 155 15 105 537
Cuts at: 5449 5909 6207 6884 8032 8277 1606
Size: 460 298 677 1148 245 2120
Fragments arranged by size:
2120 1359 1148 1105 677 537 460 454
298 245 155 113 105 15
NaeI GCC'GGC
Cuts at: 2981 4075 2981
Size: 1094 7697
NarI GG'CG_CC
Cuts at: 5848 5848
Size: 8791
NcoI C'CATG_G
Cuts at: 513 1065 3195 3327 4716 6024 513
Size: 552 2130 132 1389 1308 3280
Fragments arranged by size:
3280 2130 1389 1308 552 132
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8791
NgoMIV G'CCGG_C
Cuts at: 2979 4073 2979
Size: 1094 7697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8791
NotI GC'GGCC_GC
Cuts at: 6238 6238
Size: 8791
NspI r_CATG'y
Cuts at: 1132 1229 6149 7694 1132
Size: 97 4920 1545 2229
Fragments arranged by size:
4920 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2024 5047 1348
Size: 676 3023 5092
Fragments arranged by size:
5092 3023 676
PciI A'CATG_T
Cuts at: 7690 7690
Size: 8791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3319 3535 1108
Size: 2211 216 6364
Fragments arranged by size:
6364 2211 216
PfoI T'CCnGG_A
Cuts at: 2382 3342 3546 4425 2382
Size: 960 204 879 6748
Fragments arranged by size:
6748 960 879 204
PmeI GTTT'AAAC
Cuts at: 6095 6095
Size: 8791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5588 5620 6860 6892 8373 8405 5588
Size: 32 1240 32 1481 32 5974
Fragments arranged by size:
5974 1481 1240 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5581 5581
Size: 8791
PshAI GACnn'nnGTC
Cuts at: 1613 2714 1613
Size: 1101 7690
PsiI TTA'TAA
Cuts at: 6361 6361
Size: 8791
PspOMI G'GGCC_C
Cuts at: 5523 5826 5523
Size: 303 8488
PstI C_TGCA'G
Cuts at: 830 2748 3077 4803 4812 4917 6143 830
Size: 1918 329 1726 9 105 1226 3478
Fragments arranged by size:
3478 1918 1726 1226 329 105 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8791
PvuII CAG'CTG
Cuts at: 4792 4807 4912 5909 4792
Size: 15 105 997 7674
Fragments arranged by size:
7674 997 105 15
SacI G_AGCT'C
Cuts at: 3546 4121 5948 3546
Size: 575 1827 6389
Fragments arranged by size:
6389 1827 575
SalI G'TCGA_C
Cuts at: 1936 6133 1936
Size: 4197 4594
SapI GCTCTTCn'nnn_
Cuts at: 2477 3035 3773 4746 2477
Size: 558 738 973 6522
Fragments arranged by size:
6522 973 738 558
SbfI CC_TGCA'GG
Cuts at: 6143 6143
Size: 8791
ScaI AGT'ACT
Cuts at: 7039 8751 7039
Size: 1712 7079
SfcI C'TryA_G
Cuts at: 826 1045 2744 3073 4799 4808 4913 6139 7274
Size: 219 1699 329 1726 9 105 1226 1135
Cuts at: 7274 7955 8146 826
Size: 681 191 1471
Fragments arranged by size:
1726 1699 1471 1226 1135 681 329 219
191 105 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 5825 5825
Size: 8791
SfoI GGC'GCC
Cuts at: 5849 5849
Size: 8791
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8791
SmaI CCC'GGG
Cuts at: 1872 4198 6118 1872
Size: 2326 1920 4545
Fragments arranged by size:
4545 2326 1920
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 2604 3065 3086 4047 6895
Size: 197 926 273 388 461 21 961 2848
Cuts at: 6895 7796 8058 820
Size: 901 262 1553
Fragments arranged by size:
2848 1553 961 926 901 461 388 273
262 197 21
SnaBI TAC'GTA
Cuts at: 493 6582 493
Size: 6089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8791
SphI G_CATG'C
Cuts at: 1132 6149 1132
Size: 5017 3774
SspI AAT'ATT
Cuts at: 5 52 6072 6715 5
Size: 47 6020 643 2081
Fragments arranged by size:
6020 2081 643 47
StuI AGG'CCT
Cuts at: 1517 5482 1517
Size: 3965 4826
StyI C'CwwG_G
Cuts at: 513 1065 3195 3327 3435 3699 4716 6024 6230
Size: 552 2130 132 108 264 1017 1308 206
Cuts at: 6230 513
Size: 3074
Fragments arranged by size:
3074 2130 1308 1017 552 264 206 132
108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2832 2836 3042 4427 4490 5036 5371
Size: 1255 1252 4 206 1385 63 546 335
Cuts at: 5371 6818 6835 6988 7173 7618 325
Size: 1447 17 153 185 445 1498
Fragments arranged by size:
1498 1447 1385 1255 1252 546 445 335
206 185 153 63 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3297 7037 8749
Size: 274 80 33 51 1443 1320 3740 1712
Cuts at: 8749 96
Size: 138
Fragments arranged by size:
3740 1712 1443 1320 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4686 4718 5346 5378 8751 8783 4686
Size: 32 628 32 3373 32 4694
Fragments arranged by size:
4694 3373 628 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2441 1316
Size: 1125 7666
XbaI T'CTAG_A
Cuts at: 6127 6127
Size: 8791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3439 3439
Size: 8791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8791
XmaI C'CCGG_G
Cuts at: 1870 4196 6116 1870
Size: 2326 1920 4545
Fragments arranged by size:
4545 2326 1920
XmnI GAAnn'nnTTC
Cuts at: 4094 6920 4094
Size: 2826 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3268 6599 276
Size: 53 83 186 936 1734 3331 2468
Fragments arranged by size:
3331 2468 1734 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoNI
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SexAI SrfI SwaI