Restriction map for
pFN21AE9665
(Circular) (Six-base) MAPSORT of: pFN21AE9665.seq Check: 6197 from: 1 to: 6721
pFN21AE9665 6721 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4064 1937
Size: 2127 4594
Acc65I G'GTAC_C
Cuts at: 4042 4042
Size: 6721
AclI AA'CG_TT
Cuts at: 1563 4848 5221 1563
Size: 3285 373 3063
Fragments arranged by size:
3285 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 3702 3714 4784 6168 6436 1632
Size: 896 1174 12 1070 1384 268 1917
Fragments arranged by size:
1917 1384 1174 1070 896 268 12
AfeI AGC'GCT
Cuts at: 3708 3708
Size: 6721
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6524
AflIII A'CryG_T
Cuts at: 1203 5620 6597 1203
Size: 4417 977 1327
Fragments arranged by size:
4417 1327 977
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 5450 5450
Size: 6721
AleI CACnn'nnGTG
Cuts at: 1166 2369 2399 3002 3272 1166
Size: 1203 30 603 270 4615
Fragments arranged by size:
4615 1203 603 270 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3693 1184
Size: 2509 4212
AlwNI CAG_nnn'CTG
Cuts at: 2089 2536 4464 6036 2089
Size: 447 1928 1572 2774
Fragments arranged by size:
2774 1928 1572 447
ApaLI G'TGCA_C
Cuts at: 3502 4778 5934 3502
Size: 1276 1156 4289
Fragments arranged by size:
4289 1276 1156
ApoI r'AATT_y
Cuts at: 1493 2211 2303 2783 2950 3090 3314 3714 4030
Size: 718 92 480 167 140 224 400 316
Cuts at: 4030 4256 4408 6517 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 718 480 400 316 226 224
167 152 140 92
AseI AT'TA_AT
Cuts at: 160 1032 5275 160
Size: 872 4243 1606
Fragments arranged by size:
4243 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 4046 1870
Size: 73 2103 4545
Fragments arranged by size:
4545 2103 73
BamHI G'GATC_C
Cuts at: 3522 3543 4051 4418 3522
Size: 21 508 367 5825
Fragments arranged by size:
5825 508 367 21
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 3461 4042 5497 618
Size: 325 217 627 266 1408 581 1455 1842
Fragments arranged by size:
1842 1455 1408 627 581 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2499 4040 1391
Size: 1108 1541 4072
Fragments arranged by size:
4072 1541 1108
BbeI G_GCGC'C
Cuts at: 2057 3465 2057
Size: 1408 5313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2718 928
Size: 971 819 4931
Fragments arranged by size:
4931 971 819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4912 4946 1903
Size: 34 2975 34 3678
Fragments arranged by size:
3678 2975 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4613 5823 4613
Size: 1210 5511
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6721
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2420 5332 136
Size: 107 122 71 631 605 748 2912 1525
Fragments arranged by size:
2912 1525 748 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6716 6716
Size: 6721
BlpI GC'TnA_GC
Cuts at: 4138 4138
Size: 6721
Bme1580I G_kGCm'C
Cuts at: 1790 3506 4782 5938 1790
Size: 1716 1276 1156 2573
Fragments arranged by size:
2573 1716 1276 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6721
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3920 4452 5410
Size: 416 53 83 399 24 2503 532 958
Cuts at: 5410 442
Size: 1753
Fragments arranged by size:
2503 1753 958 532 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2120 5381 1239
Size: 818 63 3261 2579
Fragments arranged by size:
3261 2579 818 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2445 4846 5711 6009 2354
Size: 91 2401 865 298 3066
Fragments arranged by size:
3066 2401 865 298 91
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3013 3035 5384 882
Size: 617 1514 22 2349 2219
Fragments arranged by size:
2349 2219 1514 617 22
BsaAI yAC'GTr
Cuts at: 493 1204 4512 493
Size: 711 3308 2702
Fragments arranged by size:
3308 2702 711
BsaBI GATnn'nnATC
Cuts at: 6715 6715
Size: 6721
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 2093 3462 4528
Size: 53 83 186 936 521 39 1369 1066
Cuts at: 4528 4910 5545 275
Size: 382 635 1451
Fragments arranged by size:
1451 1369 1066 936 635 521 382 186
83 53 39
BsaWI w'CCGG_w
Cuts at: 3404 5154 5552 5826 5973 6384 3404
Size: 1750 398 274 147 411 3741
Fragments arranged by size:
3741 1750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 2504 2534 3399
Size: 30 165 30 675 30 396 30 865
Cuts at: 3399 3429 1178
Size: 30 4470
Fragments arranged by size:
4470 865 675 396 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2638 2657 2783 3001 1186
Size: 1452 19 126 218 4906
Fragments arranged by size:
4906 1452 218 126 19
BseYI C'CCAG_C
Cuts at: 2070 5924 6420 2070
Size: 3854 496 2371
Fragments arranged by size:
3854 2371 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3265 1163
Size: 573 1529 4619
Fragments arranged by size:
4619 1529 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3405 4171 4932 5960 1540
Size: 275 186 1404 766 761 1028 2301
Fragments arranged by size:
2301 1404 1028 766 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2471 2499 3506 4782 4867 5938 2471
Size: 28 1007 1276 85 1071 3254
Fragments arranged by size:
3254 1276 1071 1007 85 28
BsmI GAATG_Cn'
Cuts at: 4232 4325 4232
Size: 93 6628
BsmBI CGTCTCn'nnnn_
Cuts at: 2100 3392 2100
Size: 1292 5429
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2471 2499 3506 4040 4782 4867 5938
Size: 399 681 28 1007 534 742 85 1071
Cuts at: 5938 1391
Size: 2174
Fragments arranged by size:
2174 1071 1007 742 681 534 399 85
28
BspEI T'CCGG_A
Cuts at: 6384 6384
Size: 6721
BspHI T'CATG_A
Cuts at: 4610 6340 4610
Size: 1730 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3675 4076 844
Size: 2831 401 3489
Fragments arranged by size:
3489 2831 401
BsrBI CCG'CTC
Cuts at: 1394 1823 4608 1394
Size: 429 2785 3507
Fragments arranged by size:
3507 2785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3192 5216 5390 65
Size: 1183 1944 2024 174 1396
Fragments arranged by size:
2024 1944 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5365 5552 1700
Size: 110 3555 187 2869
Fragments arranged by size:
3555 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6721
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6721
BssSI C'ACGA_G
Cuts at: 4781 5793 4781
Size: 1012 5709
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2521 794
Size: 1727 4994
BstBI TT'CG_AA
Cuts at: 3135 5525 3135
Size: 2390 4331
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6721
BstXI CCAn_nnnn'nTGG
Cuts at: 2346 3022 3071 3211 2346
Size: 676 49 140 5856
Fragments arranged by size:
5856 676 140 49
BstYI r'GATC_y
Cuts at: 1972 3522 3543 3754 4051 4418 4803 4820 6272
Size: 1550 21 211 297 367 385 17 1452
Cuts at: 6272 6358 6370 6716 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1550 1452 385 367 346 297 211
86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 2478 2847 3358 3594 513
Size: 552 939 45 429 369 511 236 3640
Fragments arranged by size:
3640 939 552 511 429 369 236 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5982
Fragments arranged by size:
5982 683 56
BtsI GCAGTG_nn'
Cuts at: 2140 2430 3398 3529 4237 5031 5051 2140
Size: 290 968 131 708 794 20 3810
Fragments arranged by size:
3810 968 794 708 290 131 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6686
DraI TTT'AAA
Cuts at: 2790 4025 2790
Size: 1235 5486
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5728 809
Size: 507 4412 1802
Fragments arranged by size:
4412 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3163 4168
Size: 54 1425 50 275 18 216 1117 1005
Cuts at: 4168 5057 6686 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1117 1005 889 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4168 1537
Size: 275 2356 4090
Fragments arranged by size:
4090 2356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 3756 4651 1353
Size: 1281 1122 895 3423
Fragments arranged by size:
3423 1281 1122 895
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5308 5682 5828 387
Size: 1064 138 3719 374 146 1280
Fragments arranged by size:
3719 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2497 2497
Size: 6721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2120 2528 3702 3714 4784 5381
Size: 393 425 63 408 1174 12 1070 597
Cuts at: 5381 6168 6436 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1174 1070 787 597 425 408 393
268 63 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3551 3551
Size: 6721
EcoO109I rG'GnC_Cy
Cuts at: 2329 2329
Size: 6721
EcoRI G'AATT_C
Cuts at: 2303 2950 4030 4408 2303
Size: 647 1080 378 4616
Fragments arranged by size:
4616 1080 647 378
EcoRV GAT'ATC
Cuts at: 3591 3591
Size: 6721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3577 3577
Size: 6721
FspI TGC'GCA
Cuts at: 5227 5227
Size: 6721
HaeII r_GCGC'y
Cuts at: 1678 2057 3465 3710 5868 1678
Size: 379 1408 245 2158 2531
Fragments arranged by size:
2531 2158 1408 379 245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 3013 3045 3098
Size: 32 135 32 506 32 903 32 53
Cuts at: 3098 3130 5362 5394 5436 5468 1373
Size: 32 2232 32 42 32 2626
Fragments arranged by size:
2626 2232 903 506 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2740 4065 4311 669
Size: 988 281 802 1325 246 3079
Fragments arranged by size:
3079 1325 988 802 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6721
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KasI G'GCGC_C
Cuts at: 2053 3461 2053
Size: 1408 5313
KpnI G_GTAC'C
Cuts at: 4046 4046
Size: 6721
MfeI C'AATT_G
Cuts at: 2586 4320 2586
Size: 1734 4987
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3192 5835 6019 1305
Size: 1887 2643 184 2007
Fragments arranged by size:
2643 2007 1887 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3165 10
Size: 54 1425 343 1333 3566
Fragments arranged by size:
3566 1425 1333 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2344 2367 2369 2399 3002 3020 3272
Size: 648 1178 23 2 30 603 18 252
Cuts at: 3272 3618 4679 5038 5197 518
Size: 346 1061 359 159 2042
Fragments arranged by size:
2042 1178 1061 648 603 359 346 252
159 30 23 18 2
MspA1I CmG'CkG
Cuts at: 1606 2045 2312 2985 4137 4814 5962 6207 1606
Size: 439 267 673 1152 677 1148 245 2120
Fragments arranged by size:
2120 1152 1148 677 673 439 267 245
NarI GG'CG_CC
Cuts at: 2054 3462 2054
Size: 1408 5313
NcoI C'CATG_G
Cuts at: 513 1065 2004 2049 2478 2847 3594 513
Size: 552 939 45 429 369 747 3640
Fragments arranged by size:
3640 939 747 552 429 369 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6721
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6721
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 2597 4079 5624 1132
Size: 97 1368 1482 1545 2229
Fragments arranged by size:
2229 1545 1482 1368 97
PasI CC'CwG_GG
Cuts at: 1348 3491 1348
Size: 2143 4578
PciI A'CATG_T
Cuts at: 5620 5620
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3172 1108
Size: 2064 4657
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2433 2465 4790 4822 6303 6335 2433
Size: 32 2325 32 1481 32 2819
Fragments arranged by size:
2819 2325 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2329 2329
Size: 6721
PshAI GACnn'nnGTC
Cuts at: 1613 3407 1613
Size: 1794 4927
PsiI TTA'TAA
Cuts at: 4291 4291
Size: 6721
PstI C_TGCA'G
Cuts at: 830 2532 3393 4073 830
Size: 1702 861 680 3478
Fragments arranged by size:
3478 1702 861 680
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
PvuII CAG'CTG
Cuts at: 2312 2312
Size: 6721
SacI G_AGCT'C
Cuts at: 2499 2499
Size: 6721
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SapI GCTCTTCn'nnn_
Cuts at: 3756 3756
Size: 6721
SbfI CC_TGCA'GG
Cuts at: 2532 4073 2532
Size: 1541 5180
ScaI AGT'ACT
Cuts at: 4969 6681 4969
Size: 1712 5009
SexAI A'CCwGG_T
Cuts at: 2158 2158
Size: 6721
SfcI C'TryA_G
Cuts at: 826 1045 2528 3333 3389 4069 5204 5885 6076
Size: 219 1483 805 56 680 1135 681 191
Cuts at: 6076 826
Size: 1471
Fragments arranged by size:
1483 1471 1135 805 681 680 219 191
56
SfoI GGC'GCC
Cuts at: 2055 3463 2055
Size: 1408 5313
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6721
SmaI CCC'GGG
Cuts at: 1872 4048 1872
Size: 2176 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2333 2460 4825 5726 5988 820
Size: 197 926 390 127 2365 901 262 1553
Fragments arranged by size:
2365 1553 926 901 390 262 197 127
SnaBI TAC'GTA
Cuts at: 493 4512 493
Size: 4019 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6721
SphI G_CATG'C
Cuts at: 1132 4079 1132
Size: 2947 3774
SspI AAT'ATT
Cuts at: 5 52 4645 5
Size: 47 4593 2081
Fragments arranged by size:
4593 2081 47
StuI AGG'CCT
Cuts at: 1517 2288 1517
Size: 771 5950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2478 2847 3465 3594 4160
Size: 552 939 45 429 369 618 129 566
Cuts at: 4160 513
Size: 3074
Fragments arranged by size:
3074 939 618 566 552 429 369 129
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2530 2730 4748 4765 4918 5103 5548
Size: 1255 950 200 2018 17 153 185 445
Cuts at: 5548 325
Size: 1498
Fragments arranged by size:
2018 1498 1255 950 445 200 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4967 6679 96
Size: 274 80 33 51 1443 2990 1712 138
Fragments arranged by size:
2990 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3399 3431 6681 6713 3399
Size: 32 3250 32 3407
Fragments arranged by size:
3407 3250 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6721
XbaI T'CTAG_A
Cuts at: 3757 4057 3757
Size: 300 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3592 3592
Size: 6721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 4046 1870
Size: 2176 4545
XmnI GAAnn'nnTTC
Cuts at: 3977 4850 3977
Size: 873 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI