Restriction map for
pFN21AE9661
(Circular) (Six-base) MAPSORT of: pFN21AE9661.seq Check: 274 from: 1 to: 5470
pFN21AE9661 5470 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3280 278
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 1937
Size: 876 4594
Acc65I G'GTAC_C
Cuts at: 2791 2791
Size: 5470
AclI AA'CG_TT
Cuts at: 1563 3597 3970 1563
Size: 2034 373 3063
Fragments arranged by size:
3063 2034 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2073 3533 4917 5185 1632
Size: 441 1460 1384 268 1917
Fragments arranged by size:
1917 1460 1384 441 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5273
AflIII A'CryG_T
Cuts at: 1203 4369 5346 1203
Size: 3166 977 1327
Fragments arranged by size:
3166 1327 977
AgeI A'CCGG_T
Cuts at: 4301 4301
Size: 5470
AhdI GACnn_n'nnGTC
Cuts at: 4199 4199
Size: 5470
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5470
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5470
AlwNI CAG_nnn'CTG
Cuts at: 2187 2453 3213 4785 2187
Size: 266 760 1572 2872
Fragments arranged by size:
2872 1572 760 266
ApaLI G'TGCA_C
Cuts at: 3527 4683 3527
Size: 1156 4314
ApoI r'AATT_y
Cuts at: 1493 2779 3005 3157 5266 1493
Size: 1286 226 152 2109 1697
Fragments arranged by size:
2109 1697 1286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4024 160
Size: 872 2992 1606
Fragments arranged by size:
2992 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
AvaI C'yCGr_G
Cuts at: 1870 1943 2795 1870
Size: 73 852 4545
Fragments arranged by size:
4545 852 73
BamHI G'GATC_C
Cuts at: 2800 3167 2800
Size: 367 5103
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2791 4246 618
Size: 325 217 627 1004 1455 1842
Fragments arranged by size:
1842 1455 1004 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2789 1391
Size: 1398 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3661 3695 1903
Size: 34 1724 34 3678
Fragments arranged by size:
3678 1724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3362 4572 3362
Size: 1210 4260
BclI T'GATC_A
Cuts at: 1548 2584 1548
Size: 1036 4434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4081 136
Size: 107 122 71 631 605 2409 1525
Fragments arranged by size:
2409 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5465 5465
Size: 5470
BlpI GC'TnA_GC
Cuts at: 2688 2887 2688
Size: 199 5271
Bme1580I G_kGCm'C
Cuts at: 1790 3531 4687 1790
Size: 1741 1156 2573
Fragments arranged by size:
2573 1741 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3201 4159 442
Size: 416 53 83 399 24 1784 958 1753
Fragments arranged by size:
1784 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4130 1239
Size: 2891 2579
Bpu10I CC'TnA_GC
Cuts at: 2597 2597
Size: 5470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3595 4460 4758 3595
Size: 865 298 4307
Fragments arranged by size:
4307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2070 4133 882
Size: 617 571 2063 2219
Fragments arranged by size:
2219 2063 617 571
BsaAI yAC'GTr
Cuts at: 493 1204 3261 493
Size: 711 2057 2702
Fragments arranged by size:
2702 2057 711
BsaBI GATnn'nnATC
Cuts at: 5464 5464
Size: 5470
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3277 3659 4294 275
Size: 53 83 186 936 1744 382 635 1451
Fragments arranged by size:
1744 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3903 4301 4575 4722 5133 3903
Size: 398 274 147 411 4240
Fragments arranged by size:
4240 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5245
Fragments arranged by size:
5245 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2222 1186
Size: 889 147 4434
Fragments arranged by size:
4434 889 147
BseYI C'CCAG_C
Cuts at: 4673 5169 4673
Size: 496 4974
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4897
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2920 3681 4709 1540
Size: 275 186 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3531 3616 4687 3531
Size: 85 1071 4314
Fragments arranged by size:
4314 1071 85
BsmI GAATG_Cn'
Cuts at: 2981 3074 2981
Size: 93 5377
BsmBI CGTCTCn'nnnn_
Cuts at: 2738 2738
Size: 5470
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2789 3531 3616 4687 1391
Size: 399 999 742 85 1071 2174
Fragments arranged by size:
2174 1071 999 742 399 85
BspEI T'CCGG_A
Cuts at: 5133 5133
Size: 5470
BspHI T'CATG_A
Cuts at: 3359 5089 3359
Size: 1730 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2825 844
Size: 1981 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3357 1394
Size: 429 1534 3507
Fragments arranged by size:
3507 1534 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3965 4139 65
Size: 1183 2717 174 1396
Fragments arranged by size:
2717 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4114 4301 1700
Size: 110 2304 187 2869
Fragments arranged by size:
2869 2304 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5470
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5470
BssSI C'ACGA_G
Cuts at: 3530 4542 3530
Size: 1012 4458
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5470
BstBI TT'CG_AA
Cuts at: 4274 4274
Size: 5470
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5470
BstYI r'GATC_y
Cuts at: 1972 2140 2305 2800 3167 3552 3569 5021 5107
Size: 168 165 495 367 385 17 1452 86
Cuts at: 5107 5119 5465 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 495 385 367 346 168 165
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3979
Fragments arranged by size:
3979 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4731
Fragments arranged by size:
4731 683 56
BtsI GCAGTG_nn'
Cuts at: 2986 3780 3800 2986
Size: 794 20 4656
Fragments arranged by size:
4656 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5435
DraI TTT'AAA
Cuts at: 2101 2605 2774 2101
Size: 504 169 4797
Fragments arranged by size:
4797 504 169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4477 809
Size: 507 3161 1802
Fragments arranged by size:
3161 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2917 3806 5435
Size: 54 1425 50 275 18 1087 889 1629
Cuts at: 5435 8
Size: 43
Fragments arranged by size:
1629 1425 1087 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2917 1537
Size: 275 1105 4090
Fragments arranged by size:
4090 1105 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 3400 1353
Size: 699 1348 3423
Fragments arranged by size:
3423 1348 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4057 4431 4577 387
Size: 1064 138 2468 374 146 1280
Fragments arranged by size:
2468 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 3533 4130 4917 5185 1239
Size: 393 441 1460 597 787 268 1524
Fragments arranged by size:
1524 1460 787 597 441 393 268
EcoRI G'AATT_C
Cuts at: 2779 3157 2779
Size: 378 5092
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2444 2444
Size: 5470
FspI TGC'GCA
Cuts at: 3976 3976
Size: 5470
HaeII r_GCGC'y
Cuts at: 1678 4617 1678
Size: 2939 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 4111 4143 4185
Size: 32 135 32 629 32 1878 32 42
Cuts at: 4185 4217 1373
Size: 32 2626
Fragments arranged by size:
2626 1878 629 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2247 2814 3060 669
Size: 988 281 309 567 246 3079
Fragments arranged by size:
3079 988 567 309 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5470
HpaI GTT'AAC
Cuts at: 2247 3060 2247
Size: 813 4657
KpnI G_GTAC'C
Cuts at: 2795 2795
Size: 5470
MfeI C'AATT_G
Cuts at: 3069 3069
Size: 5470
MluI A'CGCG_T
Cuts at: 5346 5346
Size: 5470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2254 4584 4768 1305
Size: 949 2330 184 2007
Fragments arranged by size:
2330 2007 949 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3648
Fragments arranged by size:
3648 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3428 3787 3946 518
Size: 648 2262 359 159 2042
Fragments arranged by size:
2262 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2489 2886 3563 4711 4956 1606
Size: 883 397 677 1148 245 2120
Fragments arranged by size:
2120 1148 883 677 397 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3979
Fragments arranged by size:
3979 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5470
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5470
NotI GC'GGCC_GC
Cuts at: 2917 2917
Size: 5470
NspI r_CATG'y
Cuts at: 1132 1229 2828 4373 1132
Size: 97 1599 1545 2229
Fragments arranged by size:
2229 1599 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5470
PciI A'CATG_T
Cuts at: 4369 4369
Size: 5470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2086 1108
Size: 978 4492
PmeI GTTT'AAAC
Cuts at: 2774 2774
Size: 5470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 5052 5084 3539
Size: 32 1481 32 3925
Fragments arranged by size:
3925 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5470
PsiI TTA'TAA
Cuts at: 3040 3040
Size: 5470
PstI C_TGCA'G
Cuts at: 830 2034 2822 830
Size: 1204 788 3478
Fragments arranged by size:
3478 1204 788
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5470
PvuII CAG'CTG
Cuts at: 2489 2489
Size: 5470
SalI G'TCGA_C
Cuts at: 1936 2812 1936
Size: 876 4594
SbfI CC_TGCA'GG
Cuts at: 2822 2822
Size: 5470
ScaI AGT'ACT
Cuts at: 3718 5430 3718
Size: 1712 3758
SfcI C'TryA_G
Cuts at: 826 1045 2030 2818 3953 4634 4825 826
Size: 219 985 788 1135 681 191 1471
Fragments arranged by size:
1471 1135 985 788 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5470
SmaI CCC'GGG
Cuts at: 1872 2797 1872
Size: 925 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3574 4475 4737 820
Size: 197 926 1631 901 262 1553
Fragments arranged by size:
1631 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3261 493
Size: 2768 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5470
SphI G_CATG'C
Cuts at: 1132 2828 1132
Size: 1696 3774
SspI AAT'ATT
Cuts at: 5 52 3394 5
Size: 47 3342 2081
Fragments arranged by size:
3342 2081 47
StuI AGG'CCT
Cuts at: 1517 2766 1517
Size: 1249 4221
StyI C'CwwG_G
Cuts at: 513 1065 2004 2909 513
Size: 552 939 905 3074
Fragments arranged by size:
3074 939 905 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3497 3514 3667 3852 4297 325
Size: 1255 1917 17 153 185 445 1498
Fragments arranged by size:
1917 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3716 5428 96
Size: 274 80 33 51 1443 1739 1712 138
Fragments arranged by size:
1739 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5430 5462 5430
Size: 32 5438
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5470
XbaI T'CTAG_A
Cuts at: 2806 2806
Size: 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5470
XmaI C'CCGG_G
Cuts at: 1870 2795 1870
Size: 925 4545
XmnI GAAnn'nnTTC
Cuts at: 3599 3599
Size: 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3278 276
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI