Restriction map for
pFN21AE9658
(Circular) (Six-base) MAPSORT of: pFN21AE9658.seq Check: 8873 from: 1 to: 5338
pFN21AE9658 5338 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2585 3148 278
Size: 53 83 186 936 1049 563 2468
Fragments arranged by size:
2468 1049 936 563 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2112 2681 1937
Size: 175 569 4594
Fragments arranged by size:
4594 569 175
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 3401 4785 5053 1632
Size: 776 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 776 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 3081 4653 3081
Size: 1572 3766
ApaLI G'TGCA_C
Cuts at: 2453 3395 4551 2453
Size: 942 1156 3240
Fragments arranged by size:
3240 1156 942
ApoI r'AATT_y
Cuts at: 1493 2294 2647 2873 3025 5134 1493
Size: 801 353 226 152 2109 1697
Fragments arranged by size:
2109 1697 801 353 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 1870
Size: 73 720 4545
Fragments arranged by size:
4545 720 73
BamHI G'GATC_C
Cuts at: 2517 2668 3035 2517
Size: 151 367 4820
Fragments arranged by size:
4820 367 151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2659 4114 618
Size: 325 217 627 872 1455 1842
Fragments arranged by size:
1842 1455 872 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2657 1391
Size: 1266 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BfrBI ATG'CAT
Cuts at: 2548 2548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3949 136
Size: 107 122 71 631 605 2277 1525
Fragments arranged by size:
2277 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2457 3399 4555 1790
Size: 667 942 1156 2573
Fragments arranged by size:
2573 1156 942 667
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2279 3069 4027
Size: 416 53 83 399 24 862 790 958
Cuts at: 4027 442
Size: 1753
Fragments arranged by size:
1753 958 862 790 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2249 3998 1239
Size: 854 156 1749 2579
Fragments arranged by size:
2579 1749 854 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2101 2611 3129 493
Size: 711 897 510 518 2702
Fragments arranged by size:
2702 897 711 518 510
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2582 3145 3527 4162
Size: 53 83 186 936 1049 563 382 635
Cuts at: 4162 275
Size: 1451
Fragments arranged by size:
1451 1049 936 635 563 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2302 2332 1178
Size: 30 165 30 899 30 4184
Fragments arranged by size:
4184 899 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5338
BseYI C'CCAG_C
Cuts at: 2313 4541 5037 2313
Size: 2228 496 2614
Fragments arranged by size:
2614 2228 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 1163
Size: 573 456 4309
Fragments arranged by size:
4309 573 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2788 3549 4577 1540
Size: 275 186 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2280 2457 3399 3484 4555 2061
Size: 219 177 942 85 1071 2844
Fragments arranged by size:
2844 1071 942 219 177 85
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2280 2457 2657 3399 3484 4555
Size: 399 271 219 177 200 742 85 1071
Cuts at: 4555 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 271 219 200 177
85
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 844
Size: 1849 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 1394
Size: 429 1402 3507
Fragments arranged by size:
3507 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2155 3982 4169 1700
Size: 110 345 1827 187 2869
Fragments arranged by size:
2869 1827 345 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 2620 1925
Size: 695 4643
BssSI C'ACGA_G
Cuts at: 2458 3398 4410 2458
Size: 940 1012 3386
Fragments arranged by size:
3386 1012 940
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2490 2517 2668 3035 3420 3437 4889 4975
Size: 518 27 151 367 385 17 1452 86
Cuts at: 4975 4987 5333 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 518 385 367 346 151 86
27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 3733
Fragments arranged by size:
3733 1053 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2418 487
Size: 683 56 1192 3407
Fragments arranged by size:
3407 1192 683 56
BtsI GCAGTG_nn'
Cuts at: 2209 2321 2854 3648 3668 2209
Size: 112 533 794 20 3879
Fragments arranged by size:
3879 794 533 112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2595 2642 2595
Size: 47 5291
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2049 2785
Size: 54 1425 50 275 18 208 11 736
Cuts at: 2785 3674 5303 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 736 275 208 54 50
43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2785 1537
Size: 275 973 4090
Fragments arranged by size:
4090 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2610 3268 1353
Size: 1257 658 3423
Fragments arranged by size:
3423 1257 658
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2540 3925 4299 4445 387
Size: 1064 138 951 1385 374 146 1280
Fragments arranged by size:
1385 1280 1064 951 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2249 2408 3401 3998 4785 5053
Size: 393 461 156 159 993 597 787 268
Cuts at: 5053 1239
Size: 1524
Fragments arranged by size:
1524 993 787 597 461 393 268 159
156
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 4485 1678
Size: 2807 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 3979 4011 4053
Size: 32 135 32 441 32 1934 32 42
Cuts at: 4053 4085 1373
Size: 32 2626
Fragments arranged by size:
2626 1934 441 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 4452 4636 1305
Size: 1260 1887 184 2007
Fragments arranged by size:
2007 1887 1260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2040 10
Size: 54 1425 343 208 3308
Fragments arranged by size:
3308 1425 343 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 3296 3655 3814 518
Size: 648 1381 749 359 159 2042
Fragments arranged by size:
2042 1381 749 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2368 2754 3431 4579 4824 1606
Size: 762 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 386 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4786
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NsiI A_TGCA'T
Cuts at: 2550 2550
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2548 2696 4241 1132
Size: 97 1319 148 1545 2229
Fragments arranged by size:
2229 1545 1319 148 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 1108
Size: 939 4399
PfoI T'CCnGG_A
Cuts at: 2070 2196 2226 2412 2070
Size: 126 30 186 4996
Fragments arranged by size:
4996 186 126 30
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2690 830
Size: 1860 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2368 2368
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SapI GCTCTTCn'nnn_
Cuts at: 2610 2610
Size: 5338
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2686 3821 4502 4693 826
Size: 219 1641 1135 681 191 1471
Fragments arranged by size:
1641 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2777 513
Size: 552 1712 3074
Fragments arranged by size:
3074 1712 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 3365 3382 3535 3720 4165 325
Size: 1255 747 1038 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1038 747 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 3584 5296
Size: 274 80 33 51 1443 168 1439 1712
Cuts at: 5296 96
Size: 138
Fragments arranged by size:
1712 1443 1439 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2302 2334 5298 5330 2302
Size: 32 2964 32 2310
Fragments arranged by size:
2964 2310 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2479 2479
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2583 3146 276
Size: 53 83 186 936 1049 563 2468
Fragments arranged by size:
2468 1049 936 563 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI