Restriction map for
pFN21AE9656
(Circular) (Six-base) MAPSORT of: pFN21AE9656.seq Check: 4168 from: 1 to: 5197
pFN21AE9656 5197 bp
With 182 enzymes: SgfI *
March 10, 2017 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3007 278
Size: 53 83 186 936 1471 2468
Fragments arranged by size:
2468 1471 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2206 2540 1937
Size: 269 334 4594
Fragments arranged by size:
4594 334 269
Acc65I G'GTAC_C
Cuts at: 2518 2518
Size: 5197
AclI AA'CG_TT
Cuts at: 1563 3324 3697 1563
Size: 1761 373 3063
Fragments arranged by size:
3063 1761 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2365 3260 4644 4912 1632
Size: 733 895 1384 268 1917
Fragments arranged by size:
1917 1384 895 733 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5000
AflIII A'CryG_T
Cuts at: 1203 4096 5073 1203
Size: 2893 977 1327
Fragments arranged by size:
2893 1327 977
AgeI A'CCGG_T
Cuts at: 4028 4028
Size: 5197
AhdI GACnn_n'nnGTC
Cuts at: 3926 3926
Size: 5197
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5197
AlwNI CAG_nnn'CTG
Cuts at: 2940 4512 2940
Size: 1572 3625
ApaI G_GGCC'C
Cuts at: 2127 2171 2127
Size: 44 5153
ApaLI G'TGCA_C
Cuts at: 3254 4410 3254
Size: 1156 4041
ApoI r'AATT_y
Cuts at: 1493 2506 2732 2884 4993 1493
Size: 1013 226 152 2109 1697
Fragments arranged by size:
2109 1697 1013 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3751 160
Size: 872 2719 1606
Fragments arranged by size:
2719 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5197
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 2489 2522 1870
Size: 73 228 318 33 4545
Fragments arranged by size:
4545 318 228 73 33
BamHI G'GATC_C
Cuts at: 2527 2894 2527
Size: 367 4830
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2227 2485 2518 3973
Size: 325 217 627 250 190 258 33 1455
Cuts at: 3973 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 258 250 217 190
33
BanII G_rGCy'C
Cuts at: 1391 2127 2171 2516 1391
Size: 736 44 345 4072
Fragments arranged by size:
4072 736 345 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3388 3422 1903
Size: 34 1451 34 3678
Fragments arranged by size:
3678 1451 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2240 3089 4299 2240
Size: 849 1210 3138
Fragments arranged by size:
3138 1210 849
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5197
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3808 136
Size: 107 122 71 631 605 2136 1525
Fragments arranged by size:
2136 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5192 5192
Size: 5197
BlpI GC'TnA_GC
Cuts at: 2614 2614
Size: 5197
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2171 2490 3258 4414 1790
Size: 337 44 319 768 1156 2573
Fragments arranged by size:
2573 1156 768 337 319 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5197
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2928 3886 442
Size: 416 53 83 399 24 1511 958 1753
Fragments arranged by size:
1753 1511 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2426 3857 1239
Size: 1121 66 1431 2579
Fragments arranged by size:
2579 1431 1121 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3322 4187 4485 3322
Size: 865 298 4034
Fragments arranged by size:
4034 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2402 3860 882
Size: 617 903 1458 2219
Fragments arranged by size:
2219 1458 903 617
BsaAI yAC'GTr
Cuts at: 493 1204 2251 2988 493
Size: 711 1047 737 2702
Fragments arranged by size:
2702 1047 737 711
BsaBI GATnn'nnATC
Cuts at: 5191 5191
Size: 5197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 3004 3386 4021
Size: 53 83 186 936 540 931 382 635
Cuts at: 4021 275
Size: 1451
Fragments arranged by size:
1451 936 931 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3630 4028 4302 4449 4860 3630
Size: 398 274 147 411 3967
Fragments arranged by size:
3967 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2205 2235 1178
Size: 30 165 30 636 30 136 30 4140
Fragments arranged by size:
4140 636 165 136 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2342 2345 1186
Size: 1045 111 3 4038
Fragments arranged by size:
4038 1045 111 3
BseYI C'CCAG_C
Cuts at: 2456 4400 4896 2456
Size: 1944 496 2757
Fragments arranged by size:
2757 1944 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4624
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2356 2647 3408 4436 1540
Size: 275 186 355 291 761 1028 2301
Fragments arranged by size:
2301 1028 761 355 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 3258 3343 4414 3258
Size: 85 1071 4041
Fragments arranged by size:
4041 1071 85
BsmI GAATG_Cn'
Cuts at: 2708 2801 2708
Size: 93 5104
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2171 2490 2516 3258 3343 4414
Size: 399 337 44 319 26 742 85 1071
Cuts at: 4414 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 337 319 85 44
26
BspEI T'CCGG_A
Cuts at: 4860 4860
Size: 5197
BspHI T'CATG_A
Cuts at: 3086 4816 3086
Size: 1730 3467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2552 844
Size: 1708 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 2344 3084 1394
Size: 429 190 331 740 3507
Fragments arranged by size:
3507 740 429 331 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 2063 3692 3866 65
Size: 1183 815 1629 174 1396
Fragments arranged by size:
1629 1396 1183 815 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3841 4028 1700
Size: 110 2031 187 2869
Fragments arranged by size:
2869 2031 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5197
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5197
BssSI C'ACGA_G
Cuts at: 2240 3257 4269 2240
Size: 1017 1012 3168
Fragments arranged by size:
3168 1017 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2098 794
Size: 1304 3893
BstBI TT'CG_AA
Cuts at: 4001 4001
Size: 5197
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5197
BstYI r'GATC_y
Cuts at: 1972 2527 2894 3279 3296 4748 4834 4846 5192
Size: 555 367 385 17 1452 86 12 346
Cuts at: 5192 1972
Size: 1977
Fragments arranged by size:
1977 1452 555 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3706
Fragments arranged by size:
3706 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4458
Fragments arranged by size:
4458 683 56
BtsI GCAGTG_nn'
Cuts at: 2087 2370 2713 3507 3527 2087
Size: 283 343 794 20 3757
Fragments arranged by size:
3757 794 343 283 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5197
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2419 2454 529
Size: 35 1855 35 3272
Fragments arranged by size:
3272 1855 35 35
DraI TTT'AAA
Cuts at: 2501 2501
Size: 5197
DraIII CAC_nnn'GTG
Cuts at: 2397 2397
Size: 5197
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4204 809
Size: 507 2888 1802
Fragments arranged by size:
2888 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2644 3533
Size: 54 1425 50 275 18 195 619 889
Cuts at: 3533 5162 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 619 275 195 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2644 1537
Size: 275 832 4090
Fragments arranged by size:
4090 832 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 3127 1353
Size: 906 868 3423
Fragments arranged by size:
3423 906 868
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2047 2150 3784 4158 4304 387
Size: 1064 138 458 103 1634 374 146 1280
Fragments arranged by size:
1634 1280 1064 458 374 146 138 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2365 2426 3260 3857 4644 4912
Size: 393 728 5 61 834 597 787 268
Cuts at: 4912 1239
Size: 1524
Fragments arranged by size:
1524 834 787 728 597 393 268 61
5
EcoO109I rG'GnC_Cy
Cuts at: 2167 2168 2442 2167
Size: 1 274 4922
Fragments arranged by size:
4922 274 1
EcoRI G'AATT_C
Cuts at: 2506 2884 2506
Size: 378 4819
FspI TGC'GCA
Cuts at: 3703 3703
Size: 5197
HaeII r_GCGC'y
Cuts at: 1678 2143 4344 1678
Size: 465 2201 2531
Fragments arranged by size:
2531 2201 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3838 3870 3912 3944 1373
Size: 32 135 32 2266 32 42 32 2626
Fragments arranged by size:
2626 2266 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2541 2787 669
Size: 988 281 603 246 3079
Fragments arranged by size:
3079 988 603 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5197
HpaI GTT'AAC
Cuts at: 2787 2787
Size: 5197
KpnI G_GTAC'C
Cuts at: 2522 2522
Size: 5197
MfeI C'AATT_G
Cuts at: 2796 2796
Size: 5197
MluI A'CGCG_T
Cuts at: 5073 5073
Size: 5197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4311 4495 1305
Size: 3006 184 2007
Fragments arranged by size:
3006 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3375
Fragments arranged by size:
3375 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3155 3514 3673 518
Size: 648 1989 359 159 2042
Fragments arranged by size:
2042 1989 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2202 2338 2613 3290 4438 4683 1606
Size: 596 136 275 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 596 275 245 136
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3706
Fragments arranged by size:
3706 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5197
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5197
NotI GC'GGCC_GC
Cuts at: 2644 2644
Size: 5197
NspI r_CATG'y
Cuts at: 1132 1229 2555 4100 1132
Size: 97 1326 1545 2229
Fragments arranged by size:
2229 1545 1326 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5197
PciI A'CATG_T
Cuts at: 4096 4096
Size: 5197
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5197
PfoI T'CCnGG_A
Cuts at: 2309 2309
Size: 5197
PmeI GTTT'AAAC
Cuts at: 2501 2501
Size: 5197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 4779 4811 3266
Size: 32 1481 32 3652
Fragments arranged by size:
3652 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5197
PsiI TTA'TAA
Cuts at: 2767 2767
Size: 5197
PspOMI G'GGCC_C
Cuts at: 2123 2167 2123
Size: 44 5153
PstI C_TGCA'G
Cuts at: 830 2549 830
Size: 1719 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5197
SalI G'TCGA_C
Cuts at: 1936 2539 1936
Size: 603 4594
SbfI CC_TGCA'GG
Cuts at: 2549 2549
Size: 5197
ScaI AGT'ACT
Cuts at: 3445 5157 3445
Size: 1712 3485
SfcI C'TryA_G
Cuts at: 826 1045 2545 3680 4361 4552 826
Size: 219 1500 1135 681 191 1471
Fragments arranged by size:
1500 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5197
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5197
SmaI CCC'GGG
Cuts at: 1872 2524 1872
Size: 652 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3301 4202 4464 820
Size: 197 926 1358 901 262 1553
Fragments arranged by size:
1553 1358 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2988 493
Size: 2495 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5197
SphI G_CATG'C
Cuts at: 1132 2555 1132
Size: 1423 3774
SspI AAT'ATT
Cuts at: 5 52 3121 5
Size: 47 3069 2081
Fragments arranged by size:
3069 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5197
StyI C'CwwG_G
Cuts at: 513 1065 2004 2636 513
Size: 552 939 632 3074
Fragments arranged by size:
3074 939 632 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3224 3241 3394 3579 4024 325
Size: 1255 1644 17 153 185 445 1498
Fragments arranged by size:
1644 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3443 5155 96
Size: 274 80 33 51 1443 1466 1712 138
Fragments arranged by size:
1712 1466 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 2169 2201 2205 2237 5157 5189 2039
Size: 32 98 32 4 32 2920 32 2047
Fragments arranged by size:
2920 2047 98 32 32 32 32 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5197
XbaI T'CTAG_A
Cuts at: 2533 2533
Size: 5197
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5197
XmaI C'CCGG_G
Cuts at: 1870 2522 1870
Size: 652 4545
XmnI GAAnn'nnTTC
Cuts at: 3326 3326
Size: 5197
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3005 276
Size: 53 83 186 936 1471 2468
Fragments arranged by size:
2468 1471 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI