Restriction map for
pFN21AE9655
(Circular) (Six-base) MAPSORT of: pFN21AE9655.seq Check: 3310 from: 1 to: 5089
pFN21AE9655 5089 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2899 278
Size: 53 83 186 936 1363 2468
Fragments arranged by size:
2468 1363 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2432 1937
Size: 495 4594
Acc65I G'GTAC_C
Cuts at: 2410 2410
Size: 5089
AclI AA'CG_TT
Cuts at: 1563 3216 3589 1563
Size: 1653 373 3063
Fragments arranged by size:
3063 1653 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3152 4536 4804 1632
Size: 1520 1384 268 1917
Fragments arranged by size:
1917 1520 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4892
AflIII A'CryG_T
Cuts at: 1203 2140 3988 4965 1203
Size: 937 1848 977 1327
Fragments arranged by size:
1848 1327 977 937
AgeI A'CCGG_T
Cuts at: 3920 3920
Size: 5089
AhdI GACnn_n'nnGTC
Cuts at: 2130 3818 2130
Size: 1688 3401
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5089
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5089
AlwNI CAG_nnn'CTG
Cuts at: 2832 4404 2832
Size: 1572 3517
ApaLI G'TGCA_C
Cuts at: 3146 4302 3146
Size: 1156 3933
ApoI r'AATT_y
Cuts at: 1493 2398 2624 2776 4885 1493
Size: 905 226 152 2109 1697
Fragments arranged by size:
2109 1697 905 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3643 160
Size: 872 2611 1606
Fragments arranged by size:
2611 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5089
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 1870
Size: 73 471 4545
Fragments arranged by size:
4545 471 73
BamHI G'GATC_C
Cuts at: 2419 2786 2419
Size: 367 4722
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2410 3865 618
Size: 325 217 627 623 1455 1842
Fragments arranged by size:
1842 1455 627 623 325 217
BanII G_rGCy'C
Cuts at: 1391 2408 1391
Size: 1017 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2387 928
Size: 971 488 3630
Fragments arranged by size:
3630 971 488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3280 3314 1903
Size: 34 1343 34 3678
Fragments arranged by size:
3678 1343 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2981 4191 2981
Size: 1210 3879
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5089
BfrBI ATG'CAT
Cuts at: 2222 2222
Size: 5089
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3700 136
Size: 107 122 71 631 605 2028 1525
Fragments arranged by size:
2028 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5084 5084
Size: 5089
BlpI GC'TnA_GC
Cuts at: 2506 2506
Size: 5089
Bme1580I G_kGCm'C
Cuts at: 1790 3150 4306 1790
Size: 1360 1156 2573
Fragments arranged by size:
2573 1360 1156
BmgBI CAC'GTC
Cuts at: 1318 2141 1318
Size: 823 4266
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2084 2820 3778
Size: 416 53 83 399 24 667 736 958
Cuts at: 3778 442
Size: 1753
Fragments arranged by size:
1753 958 736 667 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 3749 1239
Size: 962 1548 2579
Fragments arranged by size:
2579 1548 962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3214 4079 4377 3214
Size: 865 298 3926
Fragments arranged by size:
3926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3752 882
Size: 617 2253 2219
Fragments arranged by size:
2253 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2880 493
Size: 711 1676 2702
Fragments arranged by size:
2702 1676 711
BsaBI GATnn'nnATC
Cuts at: 5083 5083
Size: 5089
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2896 3278 3913 275
Size: 53 83 186 936 1363 382 635 1451
Fragments arranged by size:
1451 1363 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2033 3522 3920 4194 4341 4752 2033
Size: 1489 398 274 147 411 2370
Fragments arranged by size:
2370 1489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4864
Fragments arranged by size:
4864 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5089
BseYI C'CCAG_C
Cuts at: 4292 4788 4292
Size: 496 4593
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2539 3300 4328 1540
Size: 275 186 538 761 1028 2301
Fragments arranged by size:
2301 1028 761 538 275 186
BsiHKAI G_wGCw'C
Cuts at: 3150 3235 4306 3150
Size: 85 1071 3933
Fragments arranged by size:
3933 1071 85
BsmI GAATG_Cn'
Cuts at: 2600 2693 2600
Size: 93 4996
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2408 3150 3235 4306 1391
Size: 399 618 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 618 399 85
BspEI T'CCGG_A
Cuts at: 2033 4752 2033
Size: 2719 2370
BspHI T'CATG_A
Cuts at: 2978 4708 2978
Size: 1730 3359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2444 844
Size: 1600 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2064 2976 1394
Size: 429 241 912 3507
Fragments arranged by size:
3507 912 429 241
BsrDI GCAATG_nn'
Cuts at: 65 1248 3584 3758 65
Size: 1183 2336 174 1396
Fragments arranged by size:
2336 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 3733 3920 1700
Size: 110 265 1658 187 2869
Fragments arranged by size:
2869 1658 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 2163 96
Size: 2067 3022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5089
BssSI C'ACGA_G
Cuts at: 3149 4161 3149
Size: 1012 4077
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2219 794
Size: 1425 3664
BstBI TT'CG_AA
Cuts at: 3893 3893
Size: 5089
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5089
BstYI r'GATC_y
Cuts at: 1972 2419 2786 3171 3188 4640 4726 4738 5084
Size: 447 367 385 17 1452 86 12 346
Cuts at: 5084 1972
Size: 1977
Fragments arranged by size:
1977 1452 447 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3598
Fragments arranged by size:
3598 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4350
Fragments arranged by size:
4350 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 3399 3419 2605
Size: 794 20 4275
Fragments arranged by size:
4275 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5089
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5054
DraI TTT'AAA
Cuts at: 2393 2393
Size: 5089
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4096 809
Size: 507 2780 1802
Fragments arranged by size:
2780 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2536 3425 5054
Size: 54 1425 50 275 18 706 889 1629
Cuts at: 5054 8
Size: 43
Fragments arranged by size:
1629 1425 889 706 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2536 1537
Size: 275 724 4090
Fragments arranged by size:
4090 724 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3019 1353
Size: 1666 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3676 4050 4196 387
Size: 1064 138 2087 374 146 1280
Fragments arranged by size:
2087 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 3152 3749 4536 4804 1239
Size: 393 569 951 597 787 268 1524
Fragments arranged by size:
1524 951 787 597 569 393 268
EcoRI G'AATT_C
Cuts at: 2398 2776 2398
Size: 378 4711
FspI TGC'GCA
Cuts at: 3595 3595
Size: 5089
HaeII r_GCGC'y
Cuts at: 1678 4236 1678
Size: 2558 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2148 3730 3762 3804
Size: 32 135 32 544 32 1582 32 42
Cuts at: 3804 3836 1373
Size: 32 2626
Fragments arranged by size:
2626 1582 544 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2433 2679 669
Size: 988 281 495 246 3079
Fragments arranged by size:
3079 988 495 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5089
HpaI GTT'AAC
Cuts at: 2679 2679
Size: 5089
KpnI G_GTAC'C
Cuts at: 2414 2414
Size: 5089
MfeI C'AATT_G
Cuts at: 2316 2688 2316
Size: 372 4717
MluI A'CGCG_T
Cuts at: 4965 4965
Size: 5089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4203 4387 1305
Size: 2898 184 2007
Fragments arranged by size:
2898 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3267
Fragments arranged by size:
3267 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2139 3047 3406 3565 518
Size: 648 973 908 359 159 2042
Fragments arranged by size:
2042 973 908 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2159 2505 3182 4330 4575 1606
Size: 553 346 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 553 346 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 5089
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3598
Fragments arranged by size:
3598 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5089
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 5089
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5089
NotI GC'GGCC_GC
Cuts at: 2536 2536
Size: 5089
NsiI A_TGCA'T
Cuts at: 2224 2224
Size: 5089
NspI r_CATG'y
Cuts at: 1132 1229 2447 3992 1132
Size: 97 1218 1545 2229
Fragments arranged by size:
2229 1545 1218 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5089
PciI A'CATG_T
Cuts at: 3988 3988
Size: 5089
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5089
PfoI T'CCnGG_A
Cuts at: 2178 2178
Size: 5089
PmeI GTTT'AAAC
Cuts at: 2393 2393
Size: 5089
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 4671 4703 3158
Size: 32 1481 32 3544
Fragments arranged by size:
3544 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5089
PsiI TTA'TAA
Cuts at: 2659 2659
Size: 5089
PstI C_TGCA'G
Cuts at: 830 2441 830
Size: 1611 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5089
SalI G'TCGA_C
Cuts at: 1936 2431 1936
Size: 495 4594
SbfI CC_TGCA'GG
Cuts at: 2441 2441
Size: 5089
ScaI AGT'ACT
Cuts at: 3337 5049 3337
Size: 1712 3377
SfcI C'TryA_G
Cuts at: 826 1045 2437 3572 4253 4444 826
Size: 219 1392 1135 681 191 1471
Fragments arranged by size:
1471 1392 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5089
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5089
SmaI CCC'GGG
Cuts at: 1872 2416 1872
Size: 544 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3193 4094 4356 820
Size: 197 926 1250 901 262 1553
Fragments arranged by size:
1553 1250 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2880 493
Size: 2387 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5089
SphI G_CATG'C
Cuts at: 1132 2447 1132
Size: 1315 3774
SspI AAT'ATT
Cuts at: 5 52 3013 5
Size: 47 2961 2081
Fragments arranged by size:
2961 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5089
StyI C'CwwG_G
Cuts at: 513 1065 2004 2528 513
Size: 552 939 524 3074
Fragments arranged by size:
3074 939 552 524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3116 3133 3286 3471 3916 325
Size: 1255 1536 17 153 185 445 1498
Fragments arranged by size:
1536 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 3335 5047
Size: 274 80 33 51 1443 186 1172 1712
Cuts at: 5047 96
Size: 138
Fragments arranged by size:
1712 1443 1172 274 186 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5049 5081 5049
Size: 32 5057
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5089
XbaI T'CTAG_A
Cuts at: 2425 2425
Size: 5089
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5089
XmaI C'CCGG_G
Cuts at: 1870 2414 1870
Size: 544 4545
XmnI GAAnn'nnTTC
Cuts at: 3218 3218
Size: 5089
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2897 276
Size: 53 83 186 936 1363 2468
Fragments arranged by size:
2468 1363 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI