Restriction map for
pFN21AE9652
(Circular) (Six-base) MAPSORT of: pFN21AE9652.seq Check: 8846 from: 1 to: 12448
pFN21AE9652 12448 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3022 5385 3022
Size: 2363 10085
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10258 278
Size: 53 83 186 936 8722 2468
Fragments arranged by size:
8722 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2932 3936 7436 7970 9462 9791 1937
Size: 995 1004 3500 534 1492 329 4594
Fragments arranged by size:
4594 3500 1492 1004 995 534 329
Acc65I G'GTAC_C
Cuts at: 9769 9769
Size: 12448
AclI AA'CG_TT
Cuts at: 1563 10575 10948 1563
Size: 9012 373 3063
Fragments arranged by size:
9012 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2831 4014 5337 5572 6060 10511 11895 12163
Size: 1199 1183 1323 235 488 4451 1384 268
Cuts at: 12163 1632
Size: 1917
Fragments arranged by size:
4451 1917 1384 1323 1199 1183 488 268
235
AfeI AGC'GCT
Cuts at: 4103 4103
Size: 12448
AflII C'TTAA_G
Cuts at: 820 1017 2489 5078 820
Size: 197 1472 2589 8190
Fragments arranged by size:
8190 2589 1472 197
AflIII A'CryG_T
Cuts at: 1203 9339 11347 12324 1203
Size: 8136 2008 977 1327
Fragments arranged by size:
8136 2008 1327 977
AgeI A'CCGG_T
Cuts at: 11279 11279
Size: 12448
AhdI GACnn_n'nnGTC
Cuts at: 6450 11177 6450
Size: 4727 7721
AleI CACnn'nnGTG
Cuts at: 1166 4664 6486 9393 1166
Size: 3498 1822 2907 4221
Fragments arranged by size:
4221 3498 2907 1822
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4401 7868 8609 1184
Size: 3217 3467 741 5023
Fragments arranged by size:
5023 3467 3217 741
AlwNI CAG_nnn'CTG
Cuts at: 2516 2572 2777 5666 7595 10191 11763 2516
Size: 56 205 2889 1929 2596 1572 3201
Fragments arranged by size:
3201 2889 2596 1929 1572 205 56
ApaI G_GGCC'C
Cuts at: 2805 9262 2805
Size: 6457 5991
ApaLI G'TGCA_C
Cuts at: 9441 10505 11661 9441
Size: 1064 1156 10228
Fragments arranged by size:
10228 1156 1064
ApoI r'AATT_y
Cuts at: 1493 2041 2182 2535 4004 4325 4634 5206 5336
Size: 548 141 353 1469 321 309 572 130
Cuts at: 5336 6257 8571 9757 9983 10135 12244 1493
Size: 921 2314 1186 226 152 2109 1697
Fragments arranged by size:
2314 2109 1697 1469 1186 921 572 548
353 321 309 226 152 141 130
AseI AT'TA_AT
Cuts at: 160 1032 5652 11002 160
Size: 872 4620 5350 1606
Fragments arranged by size:
5350 4620 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12448
AvaI C'yCGr_G
Cuts at: 1870 1943 3447 3693 5631 8030 9773 1870
Size: 73 1504 246 1938 2399 1743 4545
Fragments arranged by size:
4545 2399 1938 1743 1504 246 73
BamHI G'GATC_C
Cuts at: 6657 8946 9778 10145 6657
Size: 2289 832 367 8960
Fragments arranged by size:
8960 2289 832 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2519 3572 4347 5128 9769
Size: 325 217 627 732 1053 775 781 4641
Cuts at: 9769 11224 618
Size: 1455 1842
Fragments arranged by size:
4641 1842 1455 1053 781 775 732 627
325 217
BanII G_rGCy'C
Cuts at: 1391 2805 3252 3421 3975 4158 4233 7232 7635
Size: 1414 447 169 554 183 75 2999 403
Cuts at: 7635 9262 9544 9767 1391
Size: 1627 282 223 4072
Fragments arranged by size:
4072 2999 1627 1414 554 447 403 282
223 183 169 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5104 5608 6467 928
Size: 971 3205 504 859 6909
Fragments arranged by size:
6909 3205 971 859 504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10639 10673 1903
Size: 34 8702 34 3678
Fragments arranged by size:
8702 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4077 8149 10340 11550 4077
Size: 4072 2191 1210 4975
Fragments arranged by size:
4975 4072 2191 1210
BclI T'GATC_A
Cuts at: 1548 4773 1548
Size: 3225 9223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2528 6143 7786
Size: 107 122 71 631 605 856 3615 1643
Cuts at: 7786 11059 136
Size: 3273 1525
Fragments arranged by size:
3615 3273 1643 1525 856 631 605 122
107 71
BglII A'GATC_T
Cuts at: 5917 6000 7161 12443 5917
Size: 83 1161 5282 5922
Fragments arranged by size:
5922 5282 1161 83
BlpI GC'TnA_GC
Cuts at: 2565 9865 2565
Size: 7300 5148
Bme1580I G_kGCm'C
Cuts at: 1790 2524 2805 3427 9262 9445 10509 11665 1790
Size: 734 281 622 5835 183 1064 1156 2573
Fragments arranged by size:
5835 2573 1156 1064 734 622 281 183
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12448
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3906 4633 6836
Size: 416 53 83 399 24 2489 727 2203
Cuts at: 6836 8049 8710 9327 10179 11137 442
Size: 1213 661 617 852 958 1753
Fragments arranged by size:
2489 2203 1753 1213 958 852 727 661
617 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7180 7669 7180
Size: 489 11959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2241 2247 2422 2469 2738 2861 2913 6878
Size: 1002 6 175 47 269 123 52 3965
Cuts at: 6878 7617 8081 8213 8260 8266 8622 8640 8912
Size: 739 464 132 47 6 356 18 272
Cuts at: 8912 8957 9035 9451 9712 11108 1239
Size: 45 78 416 261 1396 2579
Fragments arranged by size:
3965 2579 1396 1002 739 464 416 356
272 269 261 175 132 123 78 52
47 47 45 18 6 6
Bpu10I CC'TnA_GC
Cuts at: 2390 3834 4809 6063 8424 2390
Size: 1444 975 1254 2361 6414
Fragments arranged by size:
6414 2361 1444 1254 975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2964 4880 5006 5024 5807 8248 9556 10573 11438
Size: 1916 126 18 783 2441 1308 1017 865
Cuts at: 11438 11736 2964
Size: 298 3676
Fragments arranged by size:
3676 2441 1916 1308 1017 865 783 298
126 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6437 6777 8933 9327 11111 882
Size: 617 4938 340 2156 394 1784 2219
Fragments arranged by size:
4938 2219 2156 1784 617 394 340
BsaAI yAC'GTr
Cuts at: 493 1204 4174 10239 493
Size: 711 2970 6065 2702
Fragments arranged by size:
6065 2970 2702 711
BsaBI GATnn'nnATC
Cuts at: 2483 7422 8841 12442 2483
Size: 4939 1419 3601 2489
Fragments arranged by size:
4939 3601 2489 1419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2345 4738 7255 10255
Size: 53 83 186 936 812 2393 2517 3000
Cuts at: 10255 10637 11272 275
Size: 382 635 1451
Fragments arranged by size:
3000 2517 2393 1451 936 812 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 6660 10881 11279 11553 11700 12111 6660
Size: 4221 398 274 147 411 6997
Fragments arranged by size:
6997 4221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3598 3628 8885 8915 9008
Size: 30 165 30 2195 30 5257 30 93
Cuts at: 9008 9038 9710 9740 1178
Size: 30 672 30 3886
Fragments arranged by size:
5257 3886 2195 672 165 93 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2425 2497 3306 3503 3557 5533 7172 7658
Size: 1239 72 809 197 54 1976 1639 486
Cuts at: 7658 8048 8696 9629 1186
Size: 390 648 933 4005
Fragments arranged by size:
4005 1976 1639 1239 933 809 648 486
390 197 72 54
BseYI C'CCAG_C
Cuts at: 2726 3096 3114 3473 3524 6532 6958 7588 11651
Size: 370 18 359 51 3008 426 630 4063
Cuts at: 11651 12147 2726
Size: 496 3027
Fragments arranged by size:
4063 3027 3008 630 496 426 370 359
51 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3056 3666 7943 8685 1163
Size: 573 1320 610 4277 742 4926
Fragments arranged by size:
4926 4277 1320 742 610 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9898 10659 11687 1540
Size: 275 186 7897 761 1028 2301
Fragments arranged by size:
7897 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3975 4262 7921 8088 9445 10509 10594 11665 3975
Size: 287 3659 167 1357 1064 85 1071 4758
Fragments arranged by size:
4758 3659 1357 1071 1064 287 167 85
BsmI GAATG_Cn'
Cuts at: 2995 3714 6756 9959 10052 2995
Size: 719 3042 3203 93 5391
Fragments arranged by size:
5391 3203 3042 719 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3718 3718
Size: 12448
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2524 2805 3252 3421 3427 3975 4158
Size: 399 734 281 447 169 6 548 183
Cuts at: 4158 4233 4262 7232 7635 7921 8088 9262 9445
Size: 75 29 2970 403 286 167 1174 183
Cuts at: 9445 9544 9767 10509 10594 11665 1391
Size: 99 223 742 85 1071 2174
Fragments arranged by size:
2970 2174 1174 1071 742 734 548 447
403 399 286 281 223 183 183 169
167 99 85 75 29 6
BspEI T'CCGG_A
Cuts at: 6660 12111 6660
Size: 5451 6997
BspHI T'CATG_A
Cuts at: 5365 10337 12067 5365
Size: 4972 1730 5746
Fragments arranged by size:
5746 4972 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3022 5222 5385 5681 5721 9803 844
Size: 2178 2200 163 296 40 4082 3489
Fragments arranged by size:
4082 3489 2200 2178 296 163 40
BsrBI CCG'CTC
Cuts at: 1394 1823 6289 8337 10335 1394
Size: 429 4466 2048 1998 3507
Fragments arranged by size:
4466 3507 2048 1998 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3408 4542 4596 4940 10943 11117 65
Size: 1183 2160 1134 54 344 6003 174 1396
Fragments arranged by size:
6003 2160 1396 1183 1134 344 174 54
BsrFI r'CCGG_y
Cuts at: 1700 1810 2624 5399 7326 11092 11279 1700
Size: 110 814 2775 1927 3766 187 2869
Fragments arranged by size:
3766 2869 2775 1927 814 187 110
BsrGI T'GTAC_A
Cuts at: 96 3136 96
Size: 3040 9408
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12448
BssSI C'ACGA_G
Cuts at: 6464 6594 8087 10508 11520 6464
Size: 130 1493 2421 1012 7392
Fragments arranged by size:
7392 2421 1493 1012 130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2920 3311 5286 6339 794
Size: 2126 391 1975 1053 6903
Fragments arranged by size:
6903 2126 1975 1053 391
BstBI TT'CG_AA
Cuts at: 11252 11252
Size: 12448
BstEII G'GTnAC_C
Cuts at: 1184 7192 1184
Size: 6008 6440
BstXI CCAn_nnnn'nTGG
Cuts at: 2162 3283 6873 2162
Size: 1121 3590 7737
Fragments arranged by size:
7737 3590 1121
BstYI r'GATC_y
Cuts at: 1972 2023 2385 5035 5917 6000 6160 6657 7081
Size: 51 362 2650 882 83 160 497 424
Cuts at: 7081 7161 8946 9778 10145 10530 10547 11999 12085
Size: 80 1785 832 367 385 17 1452 86
Cuts at: 12085 12097 12443 1972
Size: 12 346 1977
Fragments arranged by size:
2650 1977 1785 1452 882 832 497 424
385 367 362 346 160 86 83 80
51 17 12
BstZ17I GTA'TAC
Cuts at: 7971 7971
Size: 12448
Bsu36I CC'TnA_GG
Cuts at: 6764 9612 6764
Size: 2848 9600
BtgI C'CryG_G
Cuts at: 513 1065 2004 3427 4579 4672 5385 6487 7546
Size: 552 939 1423 1152 93 713 1102 1059
Cuts at: 7546 7822 8673 513
Size: 276 851 4288
Fragments arranged by size:
4288 1423 1152 1102 1059 939 851 713
552 276 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3066 487
Size: 683 56 1840 9869
Fragments arranged by size:
9869 1840 683 56
BtsI GCAGTG_nn'
Cuts at: 7952 7980 7991 9400 9602 9964 10758 10778 7952
Size: 28 11 1409 202 362 794 20 9622
Fragments arranged by size:
9622 1409 794 362 202 28 20 11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12413
DraI TTT'AAA
Cuts at: 4330 6919 8076 9752 4330
Size: 2589 1157 1676 7026
Fragments arranged by size:
7026 2589 1676 1157
DraIII CAC_nnn'GTG
Cuts at: 4921 8092 4921
Size: 3171 9277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5612 6312 8639 11455 809
Size: 507 4296 700 2327 2816 1802
Fragments arranged by size:
4296 2816 2327 1802 700 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2676 3482 4683
Size: 54 1425 50 275 18 846 806 1201
Cuts at: 4683 5449 6290 6985 7557 8097 9895 10784 12413
Size: 766 841 695 572 540 1798 889 1629
Cuts at: 12413 8
Size: 43
Fragments arranged by size:
1798 1629 1425 1201 889 846 841 806
766 695 572 540 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 9895 1537
Size: 275 8083 4090
Fragments arranged by size:
8083 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3232 3247 3512 3551 3626 3716 3798 4146
Size: 1879 15 265 39 75 90 82 348
Cuts at: 4146 5979 8307 8349 8904 9562 10378 1353
Size: 1833 2328 42 555 658 816 3423
Fragments arranged by size:
3423 2328 1879 1833 816 658 555 348
265 90 82 75 42 39 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 11035 11409 11555 387
Size: 1064 138 9446 374 146 1280
Fragments arranged by size:
9446 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3973 3973
Size: 12448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2241 2247 2422 2469 2738 2831 2861
Size: 393 609 6 175 47 269 93 30
Cuts at: 2861 2913 4014 5337 5572 6060 6878 7617 8081
Size: 52 1101 1323 235 488 818 739 464
Cuts at: 8081 8213 8260 8266 8622 8640 8912 8957 9035
Size: 132 47 6 356 18 272 45 78
Cuts at: 9035 9451 9712 10511 11108 11895 12163 1239
Size: 416 261 799 597 787 268 1524
Fragments arranged by size:
1524 1323 1101 818 799 787 739 609
597 488 464 416 393 356 272 269
268 261 235 175 132 93 78 52
47 47 45 30 18 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 5567 6767 6977 9302 5567
Size: 1200 210 2325 8713
Fragments arranged by size:
8713 2325 1200 210
EcoO109I rG'GnC_Cy
Cuts at: 2494 2686 2801 2802 2897 3237 3766 7106 7858
Size: 192 115 1 95 340 529 3340 752
Cuts at: 7858 8050 9227 2494
Size: 192 1177 5715
Fragments arranged by size:
5715 3340 1177 752 529 340 192 192
115 95 1
EcoRI G'AATT_C
Cuts at: 2182 4004 5336 6257 9757 10135 2182
Size: 1822 1332 921 3500 378 4495
Fragments arranged by size:
4495 3500 1822 1332 921 378
EcoRV GAT'ATC
Cuts at: 6640 6640
Size: 12448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6088 6727 6088
Size: 639 11809
FspI TGC'GCA
Cuts at: 5279 10954 5279
Size: 5675 6773
HaeII r_GCGC'y
Cuts at: 1678 4105 7690 11595 1678
Size: 2427 3585 3905 2531
Fragments arranged by size:
3905 3585 2531 2427
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4072 4104 4728 4760 5455
Size: 32 135 32 2500 32 624 32 695
Cuts at: 5455 5487 5902 5934 5954 5986 6383 6415 6436
Size: 32 415 32 20 32 397 32 21
Cuts at: 6436 6468 7022 7054 8198 8230 8591 8623 8675
Size: 32 554 32 1144 32 361 32 52
Cuts at: 8675 8707 9638 9670 11089 11121 11163 11195 1373
Size: 32 931 32 1419 32 42 32 2626
Fragments arranged by size:
2626 2500 1419 1144 931 695 624 554
415 397 361 135 52 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 21 20
HincII GTy'rAC
Cuts at: 669 1657 1938 7060 8793 9792 10038 669
Size: 988 281 5122 1733 999 246 3079
Fragments arranged by size:
5122 3079 1733 999 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5346 5435 6070 748
Size: 4598 89 635 7126
Fragments arranged by size:
7126 4598 635 89
HpaI GTT'AAC
Cuts at: 8793 10038 8793
Size: 1245 11203
KpnI G_GTAC'C
Cuts at: 9773 9773
Size: 12448
MfeI C'AATT_G
Cuts at: 7005 10047 7005
Size: 3042 9406
MluI A'CGCG_T
Cuts at: 12324 12324
Size: 12448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4431 5814 5914 6890 7008 9370 11562 11746
Size: 3126 1383 100 976 118 2362 2192 184
Cuts at: 11746 1305
Size: 2007
Fragments arranged by size:
3126 2362 2192 2007 1383 976 184 118
100
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4685 5451 7559 8099 10
Size: 54 1425 343 2853 766 2108 540 4359
Fragments arranged by size:
4359 2853 2108 1425 766 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2913 4146 4664 5267 6324 6464 6486
Size: 648 1747 1233 518 603 1057 140 22
Cuts at: 6486 6927 8925 9393 10406 10765 10924 518
Size: 441 1998 468 1013 359 159 2042
Fragments arranged by size:
2042 1998 1747 1233 1057 1013 648 603
518 468 441 359 159 140 22
MspA1I CmG'CkG
Cuts at: 1606 2429 2746 2965 3028 3429 5663 6536 7595
Size: 823 317 219 63 401 2234 873 1059
Cuts at: 7595 8708 9864 10541 11689 11934 1606
Size: 1113 1156 677 1148 245 2120
Fragments arranged by size:
2234 2120 1156 1148 1113 1059 873 823
677 401 317 245 219 63
NaeI GCC'GGC
Cuts at: 5401 5401
Size: 12448
NcoI C'CATG_G
Cuts at: 513 1065 2004 4579 4672 5385 7546 7822 8673
Size: 552 939 2575 93 713 2161 276 851
Cuts at: 8673 513
Size: 4288
Fragments arranged by size:
4288 2575 2161 939 851 713 552 276
93
NdeI CA'TA_TG
Cuts at: 387 5938 6833 387
Size: 5551 895 6002
Fragments arranged by size:
6002 5551 895
NgoMIV G'CCGG_C
Cuts at: 5399 5399
Size: 12448
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12448
NotI GC'GGCC_GC
Cuts at: 9895 9895
Size: 12448
NspI r_CATG'y
Cuts at: 1132 1229 2918 5248 9806 11351 1132
Size: 97 1689 2330 4558 1545 2229
Fragments arranged by size:
4558 2330 2229 1689 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2690 2789 2845 1348
Size: 1342 99 56 10951
Fragments arranged by size:
10951 1342 99 56
PciI A'CATG_T
Cuts at: 11347 11347
Size: 12448
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2891 3221 6123 7595 7773 8557 1108
Size: 1783 330 2902 1472 178 784 4999
Fragments arranged by size:
4999 2902 1783 1472 784 330 178
PfoI T'CCnGG_A
Cuts at: 8168 8617 8168
Size: 449 11999
PmeI GTTT'AAAC
Cuts at: 9752 9752
Size: 12448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10517 10549 12030 12062 10517
Size: 32 1481 32 10903
Fragments arranged by size:
10903 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2686 2897 3766 7106 7858 8050 2686
Size: 211 869 3340 752 192 7084
Fragments arranged by size:
7084 3340 869 752 211 192
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12448
PsiI TTA'TAA
Cuts at: 6702 10018 6702
Size: 3316 9132
PspOMI G'GGCC_C
Cuts at: 2801 9258 2801
Size: 6457 5991
PspXI vC'TCGA_Gb
Cuts at: 3447 3447
Size: 12448
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3249 3281 3249
Size: 32 12416
PstI C_TGCA'G
Cuts at: 830 2821 3033 3213 5382 5718 7315 8442 8698
Size: 1991 212 180 2169 336 1597 1127 256
Cuts at: 8698 9800 830
Size: 1102 3478
Fragments arranged by size:
3478 2169 1991 1597 1127 1102 336 256
212 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12448
PvuII CAG'CTG
Cuts at: 2429 2746 2965 3028 5663 6536 7595 2429
Size: 317 219 63 2635 873 1059 7282
Fragments arranged by size:
7282 2635 1059 873 317 219 63
SacI G_AGCT'C
Cuts at: 3975 3975
Size: 12448
SacII CC_GC'GG
Cuts at: 3430 3430
Size: 12448
SalI G'TCGA_C
Cuts at: 1936 9790 1936
Size: 7854 4594
SapI GCTCTTCn'nnn_
Cuts at: 3232 4146 8904 3232
Size: 914 4758 6776
Fragments arranged by size:
6776 4758 914
SbfI CC_TGCA'GG
Cuts at: 5382 5718 9800 5382
Size: 336 4082 8030
Fragments arranged by size:
8030 4082 336
ScaI AGT'ACT
Cuts at: 7541 10696 12408 7541
Size: 3155 1712 7581
Fragments arranged by size:
7581 3155 1712
SexAI A'CCwGG_T
Cuts at: 2082 2641 4245 6249 2082
Size: 559 1604 2004 8281
Fragments arranged by size:
8281 2004 1604 559
SfcI C'TryA_G
Cuts at: 826 1045 2817 3029 3209 3255 3618 4953 5066
Size: 219 1772 212 180 46 363 1335 113
Cuts at: 5066 5378 5714 7311 8438 8694 9365 9796 10931
Size: 312 336 1597 1127 256 671 431 1135
Cuts at: 10931 11612 11803 826
Size: 681 191 1471
Fragments arranged by size:
1772 1597 1471 1335 1135 1127 681 671
431 363 336 312 256 219 212 191
180 113 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12448
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12448
SmaI CCC'GGG
Cuts at: 1872 5633 9775 1872
Size: 3761 4142 4545
Fragments arranged by size:
4545 4142 3761
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 2979 3447 3693 4895 5021
Size: 197 926 546 490 468 246 1202 126
Cuts at: 5021 5039 5078 5822 8227 9535 10552 11453 11715
Size: 18 39 744 2405 1308 1017 901 262
Cuts at: 11715 820
Size: 1553
Fragments arranged by size:
2405 1553 1308 1202 1017 926 901 744
546 490 468 262 246 197 126 39
18
SnaBI TAC'GTA
Cuts at: 493 4174 10239 493
Size: 3681 6065 2702
Fragments arranged by size:
6065 3681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12448
SphI G_CATG'C
Cuts at: 1132 9806 1132
Size: 8674 3774
SspI AAT'ATT
Cuts at: 5 52 6502 10372 5
Size: 47 6450 3870 2081
Fragments arranged by size:
6450 3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 12448
StyI C'CwwG_G
Cuts at: 513 1065 2004 2113 2139 2395 2545 2682 2902
Size: 552 939 109 26 256 150 137 220
Cuts at: 2902 2995 3009 3232 4579 4672 5385 7102 7546
Size: 93 14 223 1347 93 713 1717 444
Cuts at: 7546 7822 8469 8673 9887 513
Size: 276 647 204 1214 3074
Fragments arranged by size:
3074 1717 1347 1214 939 713 647 552
444 276 256 223 220 204 150 137
109 93 93 26 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2402 3128 4365 5146 5310 8975 9425
Size: 1255 822 726 1237 781 164 3665 450
Cuts at: 9425 10475 10492 10645 10830 11275 325
Size: 1050 17 153 185 445 1498
Fragments arranged by size:
3665 1498 1255 1237 1050 822 781 726
450 445 185 164 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3136 4017 4716
Size: 274 80 33 51 1443 1159 881 699
Cuts at: 4716 6468 7539 8515 9154 10694 12406 96
Size: 1752 1071 976 639 1540 1712 138
Fragments arranged by size:
1752 1712 1540 1443 1159 1071 976 881
699 639 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3505 3537 3598 3630 7390 7422 8885 8917 12408
Size: 32 61 32 3760 32 1463 32 3491
Cuts at: 12408 12440 3505
Size: 32 3513
Fragments arranged by size:
3760 3513 3491 1463 61 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3127 8501 1316
Size: 1811 5374 5263
Fragments arranged by size:
5374 5263 1811
XbaI T'CTAG_A
Cuts at: 9170 9784 9170
Size: 614 11834
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5824 6873 5824
Size: 1049 11399
XhoI C'TCGA_G
Cuts at: 1943 3447 3693 1943
Size: 1504 246 10698
Fragments arranged by size:
10698 1504 246
XmaI C'CCGG_G
Cuts at: 1870 5631 9773 1870
Size: 3761 4142 4545
Fragments arranged by size:
4545 4142 3761
XmnI GAAnn'nnTTC
Cuts at: 2405 5055 5207 6049 7680 10577 2405
Size: 2650 152 842 1631 2897 4276
Fragments arranged by size:
4276 2897 2650 1631 842 152
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10256 276
Size: 53 83 186 936 8722 2468
Fragments arranged by size:
8722 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
RsrII SanDI SfiI SfoI SrfI SwaI