Restriction map for
pFN21AE9650
(Circular) (Six-base) MAPSORT of: pFN21AE9650.seq Check: 7042 from: 1 to: 12850
pFN21AE9650 12850 bp
With 182 enzymes: SgfI *
March 28, 2012 12:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4385 5630 9647 4385
Size: 1245 4017 7588
Fragments arranged by size:
7588 4017 1245
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6205 10660 278
Size: 53 83 186 936 4669 4455 2468
Fragments arranged by size:
4669 4455 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2189 2693 6538 10193 1937
Size: 252 504 3845 3655 4594
Fragments arranged by size:
4594 3845 3655 504 252
Acc65I G'GTAC_C
Cuts at: 10171 10171
Size: 12850
AclI AA'CG_TT
Cuts at: 1563 8028 10977 11350 1563
Size: 6465 2949 373 3063
Fragments arranged by size:
6465 3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 3315 3833 3901 3939 4784 5013 5127
Size: 585 1098 518 68 38 845 229 114
Cuts at: 5127 5862 6942 8172 8437 8732 8912 9377 9431
Size: 735 1080 1230 265 295 180 465 54
Cuts at: 9431 10913 12297 12565 1632
Size: 1482 1384 268 1917
Fragments arranged by size:
1917 1482 1384 1230 1098 1080 845 735
585 518 465 295 268 265 229 180
114 68 54 38
AfeI AGC'GCT
Cuts at: 6224 6224
Size: 12850
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12653
AflIII A'CryG_T
Cuts at: 1203 7037 8881 11749 12726 1203
Size: 5834 1844 2868 977 1327
Fragments arranged by size:
5834 2868 1844 1327 977
AgeI A'CCGG_T
Cuts at: 4533 11681 4533
Size: 7148 5702
AhdI GACnn_n'nnGTC
Cuts at: 6206 6472 8819 11579 6206
Size: 266 2347 2760 7477
Fragments arranged by size:
7477 2760 2347 266
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 12850
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 8613 9092 9655 9926 1184
Size: 7429 479 563 271 4108
Fragments arranged by size:
7429 4108 563 479 271
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 6270 6284 6302 6316 3569
Size: 32 2669 14 18 14 10103
Fragments arranged by size:
10103 2669 32 18 14 14
AlwNI CAG_nnn'CTG
Cuts at: 2650 4616 4951 9005 9698 10016 10593 12165 2650
Size: 1966 335 4054 693 318 577 1572 3335
Fragments arranged by size:
4054 3335 1966 1572 693 577 335 318
ApaI G_GGCC'C
Cuts at: 9320 9320
Size: 12850
ApaLI G'TGCA_C
Cuts at: 4576 10907 12063 4576
Size: 6331 1156 5363
Fragments arranged by size:
6331 5363 1156
ApoI r'AATT_y
Cuts at: 1493 2044 2974 3278 3928 3977 4275 4735 6255
Size: 551 930 304 650 49 298 460 1520
Cuts at: 6255 7865 9136 10159 10385 10537 12646 1493
Size: 1610 1271 1023 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 1520 1271 1023 930 650
551 460 304 298 226 152 49
AseI AT'TA_AT
Cuts at: 160 1032 7535 11404 160
Size: 872 6503 3869 1606
Fragments arranged by size:
6503 3869 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12850
AvaI C'yCGr_G
Cuts at: 1870 1943 2475 2485 2716 4148 5630 7853 8217
Size: 73 532 10 231 1432 1482 2223 364
Cuts at: 8217 8577 10175 1870
Size: 360 1598 4545
Fragments arranged by size:
4545 2223 1598 1482 1432 532 364 360
231 73 10
AvrII C'CTAG_G
Cuts at: 2222 3472 2222
Size: 1250 11600
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2262 2295 9678 9711 2262
Size: 33 7383 33 5401
Fragments arranged by size:
7383 5401 33 33
BamHI G'GATC_C
Cuts at: 3025 10180 10547 3025
Size: 7155 367 5328
Fragments arranged by size:
7155 5328 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2489 4823 5253 6869 6877
Size: 325 217 627 702 2334 430 1616 8
Cuts at: 6877 10171 11626 618
Size: 3294 1455 1842
Fragments arranged by size:
3294 2334 1842 1616 1455 702 627 430
325 217 8
BanII G_rGCy'C
Cuts at: 1391 2352 3018 3915 5199 7175 7374 7692 8064
Size: 961 666 897 1284 1976 199 318 372
Cuts at: 8064 8171 8224 8367 8758 9320 10041 10169 1391
Size: 107 53 143 391 562 721 128 4072
Fragments arranged by size:
4072 1976 1284 961 897 721 666 562
391 372 318 199 143 128 107 53
BbeI G_GCGC'C
Cuts at: 5257 5257
Size: 12850
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 3697 4941 5196 5573 6036 6384
Size: 971 1212 586 1244 255 377 463 348
Cuts at: 6384 8079 8961 9720 9986 928
Size: 1695 882 759 266 3792
Fragments arranged by size:
3792 1695 1244 1212 971 882 759 586
463 377 348 266 255
BbvCI CC'TCA_GC
Cuts at: 4226 6039 6672 9756 4226
Size: 1813 633 3084 7320
Fragments arranged by size:
7320 3084 1813 633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5274 5308 10052 10086 11041 11075 1903
Size: 34 3337 34 4744 34 955 34 3678
Fragments arranged by size:
4744 3678 3337 955 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10742 11952 10742
Size: 1210 11640
BclI T'GATC_A
Cuts at: 1548 2131 4254 5478 7012 9787 1548
Size: 583 2123 1224 1534 2775 4611
Fragments arranged by size:
4611 2775 2123 1534 1224 583
BfrBI ATG'CAT
Cuts at: 7846 7846
Size: 12850
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2849 9014 11461
Size: 107 122 71 631 605 1177 6165 2447
Cuts at: 11461 136
Size: 1525
Fragments arranged by size:
6165 2447 1525 1177 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3985 6863 7900 12845 3985
Size: 2878 1037 4945 3990
Fragments arranged by size:
4945 3990 2878 1037
BlpI GC'TnA_GC
Cuts at: 7142 8235 9294 10267 7142
Size: 1093 1059 973 9725
Fragments arranged by size:
9725 1093 1059 973
Bme1580I G_kGCm'C
Cuts at: 1790 2492 4580 5413 6671 8613 9320 9591 10075
Size: 702 2088 833 1258 1942 707 271 484
Cuts at: 10075 10105 10123 10911 12067 1790
Size: 30 18 788 1156 2573
Fragments arranged by size:
2573 2088 1942 1258 1156 833 788 707
702 484 271 30 18
BmgBI CAC'GTC
Cuts at: 1318 3718 1318
Size: 2400 10450
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2573 3583 5534
Size: 416 53 83 399 24 1156 1010 1951
Cuts at: 5534 5744 5816 6713 8699 10062 10581 11539 442
Size: 210 72 897 1986 1363 519 958 1753
Fragments arranged by size:
1986 1951 1753 1363 1156 1010 958 897
519 416 399 210 83 72 53 24
BmtI G_CTAG'C
Cuts at: 1056 5019 5618 1056
Size: 3963 599 8288
Fragments arranged by size:
8288 3963 599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6773 8611 9357 6773
Size: 1838 746 10266
Fragments arranged by size:
10266 1838 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 2847 3799 3857 3956 5498 5951 6044
Size: 1056 552 952 58 99 1542 453 93
Cuts at: 6044 6674 7785 8490 8606 9974 11510 1239
Size: 630 1111 705 116 1368 1536 2579
Fragments arranged by size:
2579 1542 1536 1368 1111 1056 952 705
630 552 453 116 99 93 58
Bpu10I CC'TnA_GC
Cuts at: 4226 4383 4671 6039 6672 9021 9089 9206 9548
Size: 157 288 1368 633 2349 68 117 342
Cuts at: 9548 9756 4226
Size: 208 7320
Fragments arranged by size:
7320 2349 1368 633 342 288 208 157
117 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3371 6647 7299 7690 8995 10975 11840 12138 3371
Size: 3276 652 391 1305 1980 865 298 4083
Fragments arranged by size:
4083 3276 1980 1305 865 652 391 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2304 3953 6744 7390 11513 882
Size: 617 805 1649 2791 646 4123 2219
Fragments arranged by size:
4123 2791 2219 1649 805 646 617
BsaAI yAC'GTr
Cuts at: 493 1204 3237 8323 10641 493
Size: 711 2033 5086 2318 2702
Fragments arranged by size:
5086 2702 2318 2033 711
BsaBI GATnn'nnATC
Cuts at: 3258 7014 12844 3258
Size: 3756 5830 3264
Fragments arranged by size:
5830 3756 3264
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3678 5254 6202 8539
Size: 53 83 186 936 2145 1576 948 2337
Cuts at: 8539 9659 10657 11039 11674 275
Size: 1120 998 382 635 1451
Fragments arranged by size:
2337 2145 1576 1451 1120 998 948 936
635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3588 4533 4872 5703 11283 11681 11955 12102 12513
Size: 945 339 831 5580 398 274 147 411
Cuts at: 12513 3588
Size: 3925
Fragments arranged by size:
5580 3925 945 831 411 398 339 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3569 3599 9001 9031 9986
Size: 30 165 30 2166 30 5402 30 955
Cuts at: 9986 10016 1178
Size: 30 4012
Fragments arranged by size:
5402 4012 2166 955 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2277 2280 2527 2693 2870 3197 3923 4013
Size: 1091 3 247 166 177 327 726 90
Cuts at: 4013 4283 7320 7616 7628 7640 8171 8872 9175
Size: 270 3037 296 12 12 531 701 303
Cuts at: 9175 9605 1186
Size: 430 4431
Fragments arranged by size:
4431 3037 1091 726 701 531 430 327
303 296 270 247 177 166 90 12
12 3
BseYI C'CCAG_C
Cuts at: 4777 5328 5914 6704 6816 6852 9065 9425 10022
Size: 551 586 790 112 36 2213 360 597
Cuts at: 10022 10040 10109 12053 12549 4777
Size: 18 69 1944 496 5078
Fragments arranged by size:
5078 2213 1944 790 597 586 551 496
360 112 69 36 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2343 2503 2805 6718 7504 8034 9625
Size: 573 607 160 302 3913 786 530 1591
Cuts at: 9625 1163
Size: 4388
Fragments arranged by size:
4388 3913 1591 786 607 573 530 302
160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4534 5143 10300 11061 12089 1540
Size: 275 186 2533 609 5157 761 1028 2301
Fragments arranged by size:
5157 2533 2301 1028 761 609 275 186
BsiHKAI G_wGCw'C
Cuts at: 2430 3018 3215 3915 4580 5199 6663 7143 7374
Size: 588 197 700 665 619 1464 480 231
Cuts at: 7374 8064 8171 8224 8981 10911 10996 12067 2430
Size: 690 107 53 757 1930 85 1071 3213
Fragments arranged by size:
3213 1930 1464 1071 757 700 690 665
619 588 480 231 197 107 85 53
BsmI GAATG_Cn'
Cuts at: 3613 10361 10454 3613
Size: 6748 93 6009
Fragments arranged by size:
6748 6009 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3669 4085 6200 8103 9666 3669
Size: 416 2115 1903 1563 6853
Fragments arranged by size:
6853 2115 1903 1563 416
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2430 2492 3018 3215 3915 4580
Size: 399 562 78 62 526 197 700 665
Cuts at: 4580 5199 5413 6663 6671 7143 7175 7374 7692
Size: 619 214 1250 8 472 32 199 318
Cuts at: 7692 8064 8171 8224 8367 8613 8758 8981 9320
Size: 372 107 53 143 246 145 223 339
Cuts at: 9320 9591 10041 10075 10105 10123 10169 10911 10996
Size: 271 450 34 30 18 46 742 85
Cuts at: 10996 12067 1391
Size: 1071 2174
Fragments arranged by size:
2174 1250 1071 742 700 665 619 562
526 472 450 399 372 339 318 271
246 223 214 199 197 145 143 107
85 78 62 53 46 34 32 30
18 8
BspEI T'CCGG_A
Cuts at: 3588 4872 5703 12513 3588
Size: 1284 831 6810 3925
Fragments arranged by size:
6810 3925 1284 831
BspHI T'CATG_A
Cuts at: 6804 9408 10739 12469 6804
Size: 2604 1331 1730 7185
Fragments arranged by size:
7185 2604 1730 1331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3994 4385 4631 5322 5630 6762 9647 9808
Size: 3150 391 246 691 308 1132 2885 161
Cuts at: 9808 10205 844
Size: 397 3489
Fragments arranged by size:
3489 3150 2885 1132 691 397 391 308
246 161
BsrBI CCG'CTC
Cuts at: 1394 1823 3322 3580 4586 5288 7799 9210 10737
Size: 429 1499 258 1006 702 2511 1411 1527
Cuts at: 10737 1394
Size: 3507
Fragments arranged by size:
3507 2511 1527 1499 1411 1006 702 429
258
BsrDI GCAATG_nn'
Cuts at: 65 1248 11345 11519 65
Size: 1183 10097 174 1396
Fragments arranged by size:
10097 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4533 5334 6246 11494 11681 1700
Size: 110 2723 801 912 5248 187 2869
Fragments arranged by size:
5248 2869 2723 912 801 187 110
BsrGI T'GTAC_A
Cuts at: 96 3669 96
Size: 3573 9277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12850
BssSI C'ACGA_G
Cuts at: 5242 8854 10910 11922 5242
Size: 3612 2056 1012 6170
Fragments arranged by size:
6170 3612 2056 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4195 794
Size: 3401 9449
BstBI TT'CG_AA
Cuts at: 11654 11654
Size: 12850
BstEII G'GTnAC_C
Cuts at: 1184 6269 1184
Size: 5085 7765
BstXI CCAn_nnnn'nTGG
Cuts at: 5782 5829 6726 7247 7775 9599 5782
Size: 47 897 521 528 1824 9033
Fragments arranged by size:
9033 1824 897 528 521 47
BstYI r'GATC_y
Cuts at: 1972 2892 3025 3259 3985 5246 5765 5817 6863
Size: 920 133 234 726 1261 519 52 1046
Cuts at: 6863 7900 8481 10180 10547 10932 10949 12401 12487
Size: 1037 581 1699 367 385 17 1452 86
Cuts at: 12487 12499 12845 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1699 1452 1261 1046 1037 920 726
581 519 385 367 346 234 133 86
52 17 12
BstZ17I GTA'TAC
Cuts at: 2190 2190
Size: 12850
Bsu36I CC'TnA_GG
Cuts at: 2676 7254 8368 8991 2676
Size: 4578 1114 623 6535
Fragments arranged by size:
6535 4578 1114 623
BtgI C'CryG_G
Cuts at: 513 1065 3529 3622 7641 7932 8605 9627 513
Size: 552 2464 93 4019 291 673 1022 3736
Fragments arranged by size:
4019 3736 2464 1022 673 552 291 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3019 487
Size: 683 56 1793 10318
Fragments arranged by size:
10318 1793 683 56
BtsI GCAGTG_nn'
Cuts at: 5677 6765 7387 7819 7947 8375 8407 9648 9699
Size: 1088 622 432 128 428 32 1241 51
Cuts at: 9699 10366 11160 11180 5677
Size: 667 794 20 7347
Fragments arranged by size:
7347 1241 1088 794 667 622 432 428
128 51 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2562 2597 6332 6367 529
Size: 35 1998 35 3735 35 7012
Fragments arranged by size:
7012 3735 1998 35 35 35
DraI TTT'AAA
Cuts at: 2049 7972 7992 10154 2049
Size: 5923 20 2162 4745
Fragments arranged by size:
5923 4745 2162 20
DraIII CAC_nnn'GTG
Cuts at: 8326 8326
Size: 12850
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4562 6961 9618 11857 809
Size: 507 3246 2399 2657 2239 1802
Fragments arranged by size:
3246 2657 2399 2239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4399 5359 6167
Size: 54 1425 50 275 18 2569 960 808
Cuts at: 6167 6513 7411 8276 10297 11186 12815 8
Size: 346 898 865 2021 889 1629 43
Fragments arranged by size:
2569 2021 1629 1425 960 898 889 865
808 346 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10297 1537
Size: 275 8485 4090
Fragments arranged by size:
8485 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2193 2504 3006 3294 3920 6288 7605
Size: 780 60 311 502 288 626 2368 1317
Cuts at: 7605 8148 8833 9210 9525 9959 10780 1353
Size: 543 685 377 315 434 821 3423
Fragments arranged by size:
3423 2368 1317 821 780 685 626 543
502 434 377 315 311 288 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2867 6169 8878 8890 11437 11811
Size: 1064 138 1278 3302 2709 12 2547 374
Cuts at: 11811 11957 387
Size: 146 1280
Fragments arranged by size:
3302 2709 2547 1280 1278 1064 374 146
138 12
EcoICRI GAG'CTC
Cuts at: 3016 3913 5197 7372 8062 8169 8222 3016
Size: 897 1284 2175 690 107 53 7644
Fragments arranged by size:
7644 2175 1284 897 690 107 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2295 2847 3315 3799 3833 3857
Size: 393 585 78 552 468 484 34 24
Cuts at: 3857 3901 3939 3956 4784 5013 5127 5498 5862
Size: 44 38 17 828 229 114 371 364
Cuts at: 5862 5951 6044 6674 6942 7785 8172 8437 8490
Size: 89 93 630 268 843 387 265 53
Cuts at: 8490 8606 8732 8912 9377 9431 9974 10913 11510
Size: 116 126 180 465 54 543 939 597
Cuts at: 11510 12297 12565 1239
Size: 787 268 1524
Fragments arranged by size:
1524 939 843 828 787 630 597 585
552 543 484 468 465 393 387 371
364 268 268 265 229 180 126 116
114 93 89 78 54 53 44 38
34 24 17
EcoNI CCTnn'n_nnAGG
Cuts at: 4172 6009 8049 9771 4172
Size: 1837 2040 1722 7251
Fragments arranged by size:
7251 2040 1837 1722
EcoO109I rG'GnC_Cy
Cuts at: 2182 2647 2752 4864 5991 8581 8679 9002 9317
Size: 465 105 2112 1127 2590 98 323 315
Cuts at: 9317 2182
Size: 5715
Fragments arranged by size:
5715 2590 2112 1127 465 323 315 105
98
EcoRI G'AATT_C
Cuts at: 10159 10537 10159
Size: 378 12472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5035 5146 6445 8512 5035
Size: 111 1299 2067 9373
Fragments arranged by size:
9373 2067 1299 111
FspI TGC'GCA
Cuts at: 11356 11356
Size: 12850
HaeII r_GCGC'y
Cuts at: 1678 5257 5278 6226 8715 11997 1678
Size: 3579 21 948 2489 3282 2531
Fragments arranged by size:
3579 3282 2531 2489 948 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 4901 4933 5210
Size: 32 135 32 515 32 2782 32 277
Cuts at: 5210 5242 5942 5974 5978 6010 6192 6224 6458
Size: 32 700 32 4 32 182 32 234
Cuts at: 6458 6490 7567 7599 8051 8083 8805 8837 9185
Size: 32 1077 32 452 32 722 32 348
Cuts at: 9185 9217 9371 9403 11491 11523 11565 11597 1373
Size: 32 154 32 2088 32 42 32 2626
Fragments arranged by size:
2782 2626 2088 1077 722 700 515 452
348 277 234 182 154 135 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 10194 10440 669
Size: 988 281 8256 246 3079
Fragments arranged by size:
8256 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3848 4355 5128 7558 7750 748
Size: 3100 507 773 2430 192 5848
Fragments arranged by size:
5848 3100 2430 773 507 192
HpaI GTT'AAC
Cuts at: 10440 10440
Size: 12850
KasI G'GCGC_C
Cuts at: 5253 5253
Size: 12850
KpnI G_GTAC'C
Cuts at: 10175 10175
Size: 12850
MfeI C'AATT_G
Cuts at: 2790 10449 2790
Size: 7659 5191
MluI A'CGCG_T
Cuts at: 12726 12726
Size: 12850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6171 6456 10158 11964 12148 1305
Size: 4866 285 3702 1806 184 2007
Fragments arranged by size:
4866 3702 2007 1806 285 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4401 6169 6515 7413 8278
Size: 54 1425 343 2569 1768 346 898 865
Cuts at: 8278 10
Size: 4582
Fragments arranged by size:
4582 2569 1768 1425 898 865 346 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4103 4217 5396 5649 5867 5883
Size: 648 843 2094 114 1179 253 218 16
Cuts at: 5883 7596 10808 11167 11326 518
Size: 1713 3212 359 159 2042
Fragments arranged by size:
3212 2094 2042 1713 1179 843 648 359
253 218 159 114 16
MspA1I CmG'CkG
Cuts at: 1606 3400 4327 4613 5747 6676 6694 6820 7417
Size: 1794 927 286 1134 929 18 126 597
Cuts at: 7417 8239 9503 10026 10044 10266 10943 12091 12336
Size: 822 1264 523 18 222 677 1148 245
Cuts at: 12336 1606
Size: 2120
Fragments arranged by size:
2120 1794 1264 1148 1134 929 927 822
677 597 523 286 245 222 126 18
18
NaeI GCC'GGC
Cuts at: 6248 6248
Size: 12850
NarI GG'CG_CC
Cuts at: 5254 5254
Size: 12850
NcoI C'CATG_G
Cuts at: 513 1065 7641 7932 9627 513
Size: 552 6576 291 1695 3736
Fragments arranged by size:
6576 3736 1695 552 291
NdeI CA'TA_TG
Cuts at: 387 3223 387
Size: 2836 10014
NgoMIV G'CCGG_C
Cuts at: 6246 6246
Size: 12850
NheI G'CTAG_C
Cuts at: 1052 5015 5614 1052
Size: 3963 599 8288
Fragments arranged by size:
8288 3963 599
NotI GC'GGCC_GC
Cuts at: 10297 10297
Size: 12850
NruI TCG'CGA
Cuts at: 3363 3363
Size: 12850
NsiI A_TGCA'T
Cuts at: 7848 7848
Size: 12850
NspI r_CATG'y
Cuts at: 1132 1229 3317 4198 7041 8885 9556 10208 11753
Size: 97 2088 881 2843 1844 671 652 1545
Cuts at: 11753 1132
Size: 2229
Fragments arranged by size:
2843 2229 2088 1844 1545 881 671 652
97
PasI CC'CwG_GG
Cuts at: 1348 3682 8960 1348
Size: 2334 5278 5238
Fragments arranged by size:
5278 5238 2334
PciI A'CATG_T
Cuts at: 7037 8881 11749 7037
Size: 1844 2868 8138
Fragments arranged by size:
8138 2868 1844
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 12850
PfoI T'CCnGG_A
Cuts at: 2546 2913 3387 5928 6828 7453 9859 2546
Size: 367 474 2541 900 625 2406 5537
Fragments arranged by size:
5537 2541 2406 900 625 474 367
PmeI GTTT'AAAC
Cuts at: 10154 10154
Size: 12850
PmlI CAC'GTG
Cuts at: 3237 8323 3237
Size: 5086 7764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 10919 10951 12432 12464 3569
Size: 32 7318 32 1481 32 3955
Fragments arranged by size:
7318 3955 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 2752 4864 5991 8679 2182
Size: 570 2112 1127 2688 6353
Fragments arranged by size:
6353 2688 2112 1127 570
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12850
PsiI TTA'TAA
Cuts at: 5439 10420 5439
Size: 4981 7869
PspOMI G'GGCC_C
Cuts at: 9316 9316
Size: 12850
PspXI vC'TCGA_Gb
Cuts at: 8217 8217
Size: 12850
PstI C_TGCA'G
Cuts at: 830 2126 2533 4020 4332 4956 5971 7382 10202
Size: 1296 407 1487 312 624 1015 1411 2820
Cuts at: 10202 830
Size: 3478
Fragments arranged by size:
3478 2820 1487 1411 1296 1015 624 407
312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12850
PvuII CAG'CTG
Cuts at: 3400 4327 4613 5747 6676 6694 6820 7417 8239
Size: 927 286 1134 929 18 126 597 822
Cuts at: 8239 9503 10026 3400
Size: 1264 523 6224
Fragments arranged by size:
6224 1264 1134 929 927 822 597 523
286 126 18
SacI G_AGCT'C
Cuts at: 3018 3915 5199 7374 8064 8171 8224 3018
Size: 897 1284 2175 690 107 53 7644
Fragments arranged by size:
7644 2175 1284 897 690 107 53
SalI G'TCGA_C
Cuts at: 1936 10192 1936
Size: 8256 4594
SanDI GG'GwC_CC
Cuts at: 8679 8679
Size: 12850
SapI GCTCTTCn'nnn_
Cuts at: 3006 3920 3006
Size: 914 11936
SbfI CC_TGCA'GG
Cuts at: 10202 10202
Size: 12850
ScaI AGT'ACT
Cuts at: 4133 4410 5691 8147 11098 12810 4133
Size: 277 1281 2456 2951 1712 4173
Fragments arranged by size:
4173 2951 2456 1712 1281 277
SexAI A'CCwGG_T
Cuts at: 3198 6265 9435 3198
Size: 3067 3170 6613
Fragments arranged by size:
6613 3170 3067
SfcI C'TryA_G
Cuts at: 826 1045 2122 2529 2619 2694 4016 4328 4952
Size: 219 1077 407 90 75 1322 312 624
Cuts at: 4952 5967 6379 6838 7378 7781 8207 8243 10198
Size: 1015 412 459 540 403 426 36 1955
Cuts at: 10198 11333 12014 12205 826
Size: 1135 681 191 1471
Fragments arranged by size:
1955 1471 1322 1135 1077 1015 681 624
540 459 426 412 407 403 312 219
191 90 75 36
SfoI GGC'GCC
Cuts at: 5255 5255
Size: 12850
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12850
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12850
SmaI CCC'GGG
Cuts at: 1872 2477 10177 1872
Size: 605 7700 4545
Fragments arranged by size:
7700 4545 605
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3350 4148 6662 7314 7705 7853
Size: 197 926 1407 798 2514 652 391 148
Cuts at: 7853 8217 8974 10954 11855 12117 820
Size: 364 757 1980 901 262 1553
Fragments arranged by size:
2514 1980 1553 1407 926 901 798 757
652 391 364 262 197 148
SnaBI TAC'GTA
Cuts at: 493 10641 493
Size: 10148 2702
SpeI A'CTAG_T
Cuts at: 152 9812 152
Size: 9660 3190
SphI G_CATG'C
Cuts at: 1132 9556 10208 1132
Size: 8424 652 3774
Fragments arranged by size:
8424 3774 652
SspI AAT'ATT
Cuts at: 5 52 3513 10774 5
Size: 47 3461 7261 2081
Fragments arranged by size:
7261 3461 2081 47
StuI AGG'CCT
Cuts at: 1517 2483 4593 1517
Size: 966 2110 9774
Fragments arranged by size:
9774 2110 966
StyI C'CwwG_G
Cuts at: 513 1065 2222 2403 2421 2493 3162 3472 4173
Size: 552 1157 181 18 72 669 310 701
Cuts at: 4173 5010 6274 7641 7720 7884 7932 8000 8758
Size: 837 1264 1367 79 164 48 68 758
Cuts at: 8758 9237 9627 10289 513
Size: 479 390 662 3074
Fragments arranged by size:
3074 1367 1264 1157 837 758 701 669
662 552 479 390 310 181 164 79
72 68 48 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2940 5157 6887 8618 9640 10023 10877
Size: 1255 1360 2217 1730 1731 1022 383 854
Cuts at: 10877 10894 11047 11232 11677 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2217 1731 1730 1498 1360 1255 1022 854
445 383 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3669 4131 4408
Size: 274 80 33 51 1443 1692 462 277
Cuts at: 4408 5421 5689 5712 8145 11096 12808 96
Size: 1013 268 23 2433 2951 1712 138
Fragments arranged by size:
2951 2433 1712 1692 1443 1013 462 277
274 268 138 80 51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4344 4376 5910 5942 7412 7444 7939 7971 7996
Size: 32 1534 32 1470 32 495 32 25
Cuts at: 7996 8028 9001 9033 12810 12842 4344
Size: 32 973 32 3777 32 4352
Fragments arranged by size:
4352 3777 1534 1470 973 495 32 32
32 32 32 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 1316 2542 1316
Size: 1226 11624
XbaI T'CTAG_A
Cuts at: 4279 7193 10186 4279
Size: 2914 2993 6943
Fragments arranged by size:
6943 2993 2914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4774 5829 8807 4774
Size: 1055 2978 8817
Fragments arranged by size:
8817 2978 1055
XhoI C'TCGA_G
Cuts at: 1943 4148 7853 8217 1943
Size: 2205 3705 364 6576
Fragments arranged by size:
6576 3705 2205 364
XmaI C'CCGG_G
Cuts at: 1870 2475 10175 1870
Size: 605 7700 4545
Fragments arranged by size:
7700 4545 605
XmnI GAAnn'nnTTC
Cuts at: 2977 3288 3513 10979 2977
Size: 311 225 7466 4848
Fragments arranged by size:
7466 4848 311 225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6203 10658 276
Size: 53 83 186 936 4669 4455 2468
Fragments arranged by size:
4669 4455 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BsiWI EcoRV FseI FspAI PacI PsrI RsrII
SacII SfiI SrfI SwaI