Restriction map for
pFN21AE9645
(Circular) (Six-base) MAPSORT of: pFN21AE9645.seq Check: 2788 from: 1 to: 12181
pFN21AE9645 12181 bp
With 182 enzymes: SgfI *
February 28, 2012 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7598 8575 7598
Size: 977 11204
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9991 278
Size: 53 83 186 936 8455 2468
Fragments arranged by size:
8455 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 9130 9524 1937
Size: 7193 394 4594
Fragments arranged by size:
7193 4594 394
Acc65I G'GTAC_C
Cuts at: 9502 9502
Size: 12181
AclI AA'CG_TT
Cuts at: 1563 10308 10681 1563
Size: 8745 373 3063
Fragments arranged by size:
8745 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 2190 2208 3555 4236 4575 4656 4959
Size: 480 78 18 1347 681 339 81 303
Cuts at: 4959 4974 4992 5217 5511 5721 5862 6009 8322
Size: 15 18 225 294 210 141 147 2313
Cuts at: 8322 8711 8826 10244 11628 11896 1632
Size: 389 115 1418 1384 268 1917
Fragments arranged by size:
2313 1917 1418 1384 1347 681 480 389
339 303 294 268 225 210 147 141
115 81 78 18 18 15
AflII C'TTAA_G
Cuts at: 820 1017 3179 6494 820
Size: 197 2162 3315 6507
Fragments arranged by size:
6507 3315 2162 197
AflIII A'CryG_T
Cuts at: 1203 3976 4862 11080 12057 1203
Size: 2773 886 6218 977 1327
Fragments arranged by size:
6218 2773 1327 977 886
AgeI A'CCGG_T
Cuts at: 3470 11012 3470
Size: 7542 4639
AhdI GACnn_n'nnGTC
Cuts at: 2534 10910 2534
Size: 8376 3805
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 12181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4764 1184
Size: 3580 8601
AlwNI CAG_nnn'CTG
Cuts at: 4175 4196 4259 5768 5987 7142 7748 8030 8557
Size: 21 63 1509 219 1155 606 282 527
Cuts at: 8557 9395 9924 11496 4175
Size: 838 529 1572 4860
Fragments arranged by size:
4860 1572 1509 1155 838 606 529 527
282 219 63 21
ApaLI G'TGCA_C
Cuts at: 9426 10238 11394 9426
Size: 812 1156 10213
Fragments arranged by size:
10213 1156 812
ApoI r'AATT_y
Cuts at: 1493 2335 2438 2860 3119 3244 4186 4272 4524
Size: 842 103 422 259 125 942 86 252
Cuts at: 4524 4603 4904 5076 5419 5644 5698 6441 6914
Size: 79 301 172 343 225 54 743 473
Cuts at: 6914 7210 7306 7478 7730 7879 8079 8306 9331
Size: 296 96 172 252 149 200 227 1025
Cuts at: 9331 9490 9716 9868 11977 1493
Size: 159 226 152 2109 1697
Fragments arranged by size:
2109 1697 1025 942 842 743 473 422
343 301 296 259 252 252 227 226
225 200 172 172 159 152 149 125
103 96 86 79 54
AseI AT'TA_AT
Cuts at: 160 1032 4266 8376 8988 10735 160
Size: 872 3234 4110 612 1747 1606
Fragments arranged by size:
4110 3234 1747 1606 872 612
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12181
AvaI C'yCGr_G
Cuts at: 1870 1943 7890 9506 1870
Size: 73 5947 1616 4545
Fragments arranged by size:
5947 4545 1616 73
BamHI G'GATC_C
Cuts at: 7316 9511 9878 7316
Size: 2195 367 9619
Fragments arranged by size:
9619 2195 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 9502 10957 618
Size: 325 217 627 7715 1455 1842
Fragments arranged by size:
7715 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2019 4406 8724 8950 9500 1391
Size: 628 2387 4318 226 550 4072
Fragments arranged by size:
4318 4072 2387 628 550 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 2418 2966 3243 3588 4437 4616
Size: 971 315 204 548 277 345 849 179
Cuts at: 4616 5703 7722 8192 928
Size: 1087 2019 470 4917
Fragments arranged by size:
4917 2019 1087 971 849 548 470 345
315 277 204 179
BbvCI CC'TCA_GC
Cuts at: 5478 8037 5478
Size: 2559 9622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10372 10406 1903
Size: 34 8435 34 3678
Fragments arranged by size:
8435 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8677 8698 8747 10073 11283 8677
Size: 21 49 1326 1210 9575
Fragments arranged by size:
9575 1326 1210 49 21
BclI T'GATC_A
Cuts at: 1548 3631 5638 7041 8575 9336 1548
Size: 2083 2007 1403 1534 761 4393
Fragments arranged by size:
4393 2083 2007 1534 1403 761
BfrBI ATG'CAT
Cuts at: 2733 3427 6774 2733
Size: 694 3347 8140
Fragments arranged by size:
8140 3347 694
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10792 136
Size: 107 122 71 631 605 9120 1525
Fragments arranged by size:
9120 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7886 12176 7886
Size: 4290 7891
BlpI GC'TnA_GC
Cuts at: 2013 9598 2013
Size: 7585 4596
Bme1580I G_kGCm'C
Cuts at: 1790 3051 4599 9430 10242 11398 1790
Size: 1261 1548 4831 812 1156 2573
Fragments arranged by size:
4831 2573 1548 1261 1156 812
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4429 8892 9311
Size: 416 53 83 399 24 3012 4463 419
Cuts at: 9311 9912 10870 442
Size: 601 958 1753
Fragments arranged by size:
4463 3012 1753 958 601 419 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4211 1056
Size: 3155 9026
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4684 8703 4684
Size: 4019 8162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3091 6081 6744 7983 8996 9230 10841 1239
Size: 1852 2990 663 1239 1013 234 1611 2579
Fragments arranged by size:
2990 2579 1852 1611 1239 1013 663 234
Bpu10I CC'TnA_GC
Cuts at: 4137 5064 5478 8037 8505 4137
Size: 927 414 2559 468 7813
Fragments arranged by size:
7813 2559 927 468 414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3620 4015 6977 7059 7816 9074 10306 11171 11469
Size: 395 2962 82 757 1258 1232 865 298
Cuts at: 11469 3620
Size: 4332
Fragments arranged by size:
4332 2962 1258 1232 865 757 395 298
82
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3080 10844 882
Size: 617 1581 7764 2219
Fragments arranged by size:
7764 2219 1581 617
BsaAI yAC'GTr
Cuts at: 493 1204 3977 9972 493
Size: 711 2773 5995 2702
Fragments arranged by size:
5995 2773 2702 711
BsaBI GATnn'nnATC
Cuts at: 5637 9206 12175 5637
Size: 3569 2969 5643
Fragments arranged by size:
5643 3569 2969
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 9988 10370 11005 275
Size: 53 83 186 936 8455 382 635 1451
Fragments arranged by size:
8455 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3470 10614 11012 11286 11433 11844 3470
Size: 7144 398 274 147 411 3807
Fragments arranged by size:
7144 3807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2089 2119 3089 3119 1178
Size: 30 165 30 686 30 970 30 10240
Fragments arranged by size:
10240 970 686 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2097 3732 4802 6356 6371 6374 6383 6386
Size: 911 1635 1070 1554 15 3 9 3
Cuts at: 6386 6389 6392 6401 6404 7243 1186
Size: 3 3 9 3 839 6124
Fragments arranged by size:
6124 1635 1554 1070 911 839 15 9
9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 7993 11384 11880 7993
Size: 3391 496 8294
Fragments arranged by size:
8294 3391 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2500 6674 7392 8262 1163
Size: 573 333 431 4174 718 870 5082
Fragments arranged by size:
5082 4174 870 718 573 431 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4004 9631 10392 11420 1540
Size: 275 186 2003 5627 761 1028 2301
Fragments arranged by size:
5627 2301 2003 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3423 4406 7417 8724 9430 10242 10327 11398 3423
Size: 983 3011 1307 706 812 85 1071 4206
Fragments arranged by size:
4206 3011 1307 1071 983 812 706 85
BsmI GAATG_Cn'
Cuts at: 2733 6774 8050 9692 9785 2733
Size: 4041 1276 1642 93 5129
Fragments arranged by size:
5129 4041 1642 1276 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6505 6505
Size: 12181
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 3051 3423 4406 4599 7417 8724
Size: 399 229 1032 372 983 193 2818 1307
Cuts at: 8724 8950 9430 9500 10242 10327 11398 1391
Size: 226 480 70 742 85 1071 2174
Fragments arranged by size:
2818 2174 1307 1071 1032 983 742 480
399 372 229 226 193 85 70
BspEI T'CCGG_A
Cuts at: 11844 11844
Size: 12181
BspHI T'CATG_A
Cuts at: 2059 9312 9339 10070 11800 2059
Size: 7253 27 731 1730 2440
Fragments arranged by size:
7253 2440 1730 731 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3529 6646 7598 8575 9536 844
Size: 2685 3117 952 977 961 3489
Fragments arranged by size:
3489 3117 2685 977 961 952
BsrBI CCG'CTC
Cuts at: 1394 1823 10068 1394
Size: 429 8245 3507
Fragments arranged by size:
8245 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 2576 8964 10676 10850 65
Size: 1183 879 449 6388 1712 174 1396
Fragments arranged by size:
6388 1712 1396 1183 879 449 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3470 10825 11012 1700
Size: 110 1660 7355 187 2869
Fragments arranged by size:
7355 2869 1660 187 110
BsrGI T'GTAC_A
Cuts at: 96 2386 96
Size: 2290 9891
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12181
BssSI C'ACGA_G
Cuts at: 10241 11253 10241
Size: 1012 11169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2365 2730 794
Size: 1571 365 10245
Fragments arranged by size:
10245 1571 365
BstBI TT'CG_AA
Cuts at: 3964 8086 8239 10985 3964
Size: 4122 153 2746 5160
Fragments arranged by size:
5160 4122 2746 153
BstEII G'GTnAC_C
Cuts at: 1184 9305 1184
Size: 8121 4060
BstXI CCAn_nnnn'nTGG
Cuts at: 2773 2773
Size: 12181
BstYI r'GATC_y
Cuts at: 1972 3968 5873 7316 7886 8363 9511 9878 10263
Size: 1996 1905 1443 570 477 1148 367 385
Cuts at: 10263 10280 11732 11818 11830 12176 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1996 1977 1905 1452 1443 1148 570 477
385 367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5091 6990 5091
Size: 1899 10282
BtgI C'CryG_G
Cuts at: 513 1065 7383 8514 513
Size: 552 6318 1131 4180
Fragments arranged by size:
6318 4180 1131 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3275 4941 5682 487
Size: 683 56 2049 1666 741 6986
Fragments arranged by size:
6986 2049 1666 741 683 56
BtsI GCAGTG_nn'
Cuts at: 2517 2835 3658 6136 7852 9042 9697 10491 10511
Size: 318 823 2478 1716 1190 655 794 20
Cuts at: 10511 2517
Size: 4187
Fragments arranged by size:
4187 2478 1716 1190 823 794 655 318
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2648 2683 529
Size: 35 2084 35 10027
Fragments arranged by size:
10027 2084 35 35
DraI TTT'AAA
Cuts at: 2428 3126 4305 7947 9485 2428
Size: 698 1179 3642 1538 5124
Fragments arranged by size:
5124 3642 1538 1179 698
DraIII CAC_nnn'GTG
Cuts at: 6594 6594
Size: 12181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11188 809
Size: 507 9872 1802
Fragments arranged by size:
9872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 9628 10517 12146
Size: 54 1425 50 275 18 7798 889 1629
Cuts at: 12146 8
Size: 43
Fragments arranged by size:
7798 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 9628 1537
Size: 275 7816 4090
Fragments arranged by size:
7816 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2209 4555 4675 5236 5306 5380 6333
Size: 831 25 2346 120 561 70 74 953
Cuts at: 6333 6348 6360 6378 6495 6501 7085 7591 8672
Size: 15 12 18 117 6 584 506 1081
Cuts at: 8672 8790 8992 9157 10111 1353
Size: 118 202 165 954 3423
Fragments arranged by size:
3423 2346 1081 954 953 831 584 561
506 202 165 120 118 117 74 70
25 18 15 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10768 11142 11288 387
Size: 1064 138 9179 374 146 1280
Fragments arranged by size:
9179 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4404 8722 4404
Size: 4318 7863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2190 2208 3091 3555 4236 4575
Size: 393 480 78 18 883 464 681 339
Cuts at: 4575 4656 4959 4974 4992 5217 5511 5721 5862
Size: 81 303 15 18 225 294 210 141
Cuts at: 5862 6009 6081 6744 7983 8322 8711 8826 8996
Size: 147 72 663 1239 339 389 115 170
Cuts at: 8996 9230 10244 10841 11628 11896 1239
Size: 234 1014 597 787 268 1524
Fragments arranged by size:
1524 1239 1014 883 787 681 663 597
480 464 393 389 339 339 303 294
268 234 225 210 170 147 141 115
81 78 72 18 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4220 6426 9465 4220
Size: 2206 3039 6936
Fragments arranged by size:
6936 3039 2206
EcoO109I rG'GnC_Cy
Cuts at: 2432 7139 8980 9458 2432
Size: 4707 1841 478 5155
Fragments arranged by size:
5155 4707 1841 478
EcoRI G'AATT_C
Cuts at: 4524 5644 7210 7879 9490 9868 4524
Size: 1120 1566 669 1611 378 6837
Fragments arranged by size:
6837 1611 1566 1120 669 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2513 2620 3410 5870 8180 2513
Size: 107 790 2460 2310 6514
Fragments arranged by size:
6514 2460 2310 790 107
FspI TGC'GCA
Cuts at: 10687 10687
Size: 12181
HaeII r_GCGC'y
Cuts at: 1678 11328 1678
Size: 9650 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2520 2552 3542 3574 3665
Size: 32 135 32 948 32 990 32 91
Cuts at: 3665 3697 4165 4197 4532 4564 4831 4863 4871
Size: 32 468 32 335 32 267 32 8
Cuts at: 4871 4903 5168 5200 5312 5344 5591 5623 5858
Size: 32 265 32 112 32 247 32 235
Cuts at: 5858 5890 6041 6073 6401 6433 7742 7774 7814
Size: 32 151 32 328 32 1309 32 40
Cuts at: 7814 7846 8328 8360 8518 8550 9034 9066 9156
Size: 32 482 32 158 32 484 32 90
Cuts at: 9156 9188 9328 9360 10822 10854 10896 10928 1373
Size: 32 140 32 1462 32 42 32 2626
Fragments arranged by size:
2626 1462 1309 990 948 484 482 468
335 328 267 265 247 235 158 151
140 135 112 91 90 42 40 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2905 5205 8802 9525 9771 669
Size: 988 281 967 2300 3597 723 246 3079
Fragments arranged by size:
3597 3079 2300 988 967 723 281 246
HindIII A'AGCT_T
Cuts at: 748 2054 4772 6953 7122 7616 748
Size: 1306 2718 2181 169 494 5313
Fragments arranged by size:
5313 2718 2181 1306 494 169
HpaI GTT'AAC
Cuts at: 9771 9771
Size: 12181
KpnI G_GTAC'C
Cuts at: 9506 9506
Size: 12181
MfeI C'AATT_G
Cuts at: 2256 9780 2256
Size: 7524 4657
MluI A'CGCG_T
Cuts at: 12057 12057
Size: 12181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2805 3464 6219 7076 11295 11479 1305
Size: 1500 659 2755 857 4219 184 2007
Fragments arranged by size:
4219 2755 2007 1500 857 659 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 10359
Fragments arranged by size:
10359 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2771 3432 7500 8763 10139 10498 10657
Size: 648 1605 661 4068 1263 1376 359 159
Cuts at: 10657 518
Size: 2042
Fragments arranged by size:
4068 2042 1605 1376 1263 661 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2012 6205 6253 8974 9071 9213 9597 10274
Size: 406 4193 48 2721 97 142 384 677
Cuts at: 10274 11422 11667 1606
Size: 1148 245 2120
Fragments arranged by size:
4193 2721 2120 1148 677 406 384 245
142 97 48
NcoI C'CATG_G
Cuts at: 513 1065 7383 8514 513
Size: 552 6318 1131 4180
Fragments arranged by size:
6318 4180 1131 552
NdeI CA'TA_TG
Cuts at: 387 5263 387
Size: 4876 7305
NheI G'CTAG_C
Cuts at: 1052 4207 1052
Size: 3155 9026
NotI GC'GGCC_GC
Cuts at: 9628 9628
Size: 12181
NsiI A_TGCA'T
Cuts at: 2735 3429 6776 2735
Size: 694 3347 8140
Fragments arranged by size:
8140 3347 694
NspI r_CATG'y
Cuts at: 1132 1229 2844 3427 4866 7009 9539 11084 1132
Size: 97 1615 583 1439 2143 2530 1545 2229
Fragments arranged by size:
2530 2229 2143 1615 1545 1439 583 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 12181
PciI A'CATG_T
Cuts at: 4862 11080 4862
Size: 6218 5963
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6790 9395 1108
Size: 5682 2605 3894
Fragments arranged by size:
5682 3894 2605
PfoI T'CCnGG_A
Cuts at: 6421 9454 6421
Size: 3033 9148
PmeI GTTT'AAAC
Cuts at: 9485 9485
Size: 12181
PmlI CAC'GTG
Cuts at: 3977 3977
Size: 12181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4228 4260 10250 10282 11763 11795 4228
Size: 32 5990 32 1481 32 4614
Fragments arranged by size:
5990 4614 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 8980 9458 8980
Size: 478 11703
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12181
PsiI TTA'TAA
Cuts at: 7346 8217 9751 7346
Size: 871 1534 9776
Fragments arranged by size:
9776 1534 871
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6690 6722 6690
Size: 32 12149
PstI C_TGCA'G
Cuts at: 830 7014 9533 830
Size: 6184 2519 3478
Fragments arranged by size:
6184 3478 2519
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12181
PvuII CAG'CTG
Cuts at: 8974 9071 9213 8974
Size: 97 142 11942
Fragments arranged by size:
11942 142 97
SacI G_AGCT'C
Cuts at: 4406 8724 4406
Size: 4318 7863
SalI G'TCGA_C
Cuts at: 1936 9523 1936
Size: 7587 4594
SapI GCTCTTCn'nnn_
Cuts at: 2209 2209
Size: 12181
SbfI CC_TGCA'GG
Cuts at: 9533 9533
Size: 12181
ScaI AGT'ACT
Cuts at: 7550 10429 12141 7550
Size: 2879 1712 7590
Fragments arranged by size:
7590 2879 1712
SfcI C'TryA_G
Cuts at: 826 1045 3738 4125 5649 7010 9529 10664 11345
Size: 219 2693 387 1524 1361 2519 1135 681
Cuts at: 11345 11536 826
Size: 191 1471
Fragments arranged by size:
2693 2519 1524 1471 1361 1135 681 387
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12181
SmaI CCC'GGG
Cuts at: 1872 9508 1872
Size: 7636 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3179 3599 3994 6494 6956 7074
Size: 197 926 1236 420 395 2500 462 118
Cuts at: 7074 7831 9053 10285 11186 11448 820
Size: 757 1222 1232 901 262 1553
Fragments arranged by size:
2500 1553 1236 1232 1222 926 901 757
462 420 395 262 197 118
SnaBI TAC'GTA
Cuts at: 493 9972 493
Size: 9479 2702
SpeI A'CTAG_T
Cuts at: 152 2963 152
Size: 2811 9370
SphI G_CATG'C
Cuts at: 1132 9539 1132
Size: 8407 3774
SspI AAT'ATT
Cuts at: 5 52 3697 5348 7113 10105 5
Size: 47 3645 1651 1765 2992 2081
Fragments arranged by size:
3645 2992 2081 1765 1651 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 12181
StyI C'CwwG_G
Cuts at: 513 1065 2746 6796 7383 7661 8514 9466 9620
Size: 552 1681 4050 587 278 853 952 154
Cuts at: 9620 513
Size: 3074
Fragments arranged by size:
4050 3074 1681 952 853 587 552 278
154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2826 4018 6601 10208 10225 10378 10563
Size: 1255 1246 1192 2583 3607 17 153 185
Cuts at: 10563 11008 325
Size: 445 1498
Fragments arranged by size:
3607 2583 1498 1255 1246 1192 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 4873 7548
Size: 274 80 33 51 1443 409 2487 2675
Cuts at: 7548 9109 9255 10427 12139 96
Size: 1561 146 1172 1712 138
Fragments arranged by size:
2675 2487 1712 1561 1443 1172 409 274
146 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6580 6612 7071 7103 12141 12173 6580
Size: 32 459 32 5038 32 6588
Fragments arranged by size:
6588 5038 459 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2831 1316
Size: 1515 10666
XbaI T'CTAG_A
Cuts at: 9517 9517
Size: 12181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6698 8001 6698
Size: 1303 10878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12181
XmaI C'CCGG_G
Cuts at: 1870 9506 1870
Size: 7636 4545
XmnI GAAnn'nnTTC
Cuts at: 4351 5302 7522 8082 8160 10310 4351
Size: 951 2220 560 78 2150 6222
Fragments arranged by size:
6222 2220 2150 951 560 78
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9989 276
Size: 53 83 186 936 8455 2468
Fragments arranged by size:
8455 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspOMI PspXI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI