Restriction map for
pFN21AE9638
(Circular) (Six-base) MAPSORT of: pFN21AE9638.seq Check: 421 from: 1 to: 10189
pFN21AE9638 10189 bp
With 182 enzymes: SgfI *
March 28, 2012 12:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7999 278
Size: 53 83 186 936 6463 2468
Fragments arranged by size:
6463 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7532 1937
Size: 5595 4594
Acc65I G'GTAC_C
Cuts at: 7510 7510
Size: 10189
AclI AA'CG_TT
Cuts at: 1563 2691 8316 8689 1563
Size: 1128 5625 373 3063
Fragments arranged by size:
5625 3063 1128 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2100 3096 3099 3407 3935 4160 4338 4620
Size: 468 996 3 308 528 225 178 282
Cuts at: 4620 5534 5745 6883 8252 9636 9904 1632
Size: 914 211 1138 1369 1384 268 1917
Fragments arranged by size:
1917 1384 1369 1138 996 914 528 468
308 282 268 225 211 178 3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9992
AflIII A'CryG_T
Cuts at: 1203 7083 9088 10065 1203
Size: 5880 2005 977 1327
Fragments arranged by size:
5880 2005 1327 977
AgeI A'CCGG_T
Cuts at: 9020 9020
Size: 10189
AhdI GACnn_n'nnGTC
Cuts at: 5272 8918 5272
Size: 3646 6543
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10189
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6923 6955 6923
Size: 32 10157
AlwNI CAG_nnn'CTG
Cuts at: 2078 3109 4513 4552 7932 9504 2078
Size: 1031 1404 39 3380 1572 2763
Fragments arranged by size:
3380 2763 1572 1404 1031 39
ApaLI G'TGCA_C
Cuts at: 8246 9402 8246
Size: 1156 9033
ApoI r'AATT_y
Cuts at: 1493 3311 3955 4010 4922 4995 5202 5220 5889
Size: 1818 644 55 912 73 207 18 669
Cuts at: 5889 6629 7193 7498 7724 7876 9985 1493
Size: 740 564 305 226 152 2109 1697
Fragments arranged by size:
2109 1818 1697 912 740 669 644 564
305 226 207 152 73 55 18
AseI AT'TA_AT
Cuts at: 160 1032 8743 160
Size: 872 7711 1606
Fragments arranged by size:
7711 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10189
AvaI C'yCGr_G
Cuts at: 1870 1943 4701 5940 6036 7514 1870
Size: 73 2758 1239 96 1478 4545
Fragments arranged by size:
4545 2758 1478 1239 96 73
AvrII C'CTAG_G
Cuts at: 3541 6101 3541
Size: 2560 7629
BamHI G'GATC_C
Cuts at: 7519 7886 7519
Size: 367 9822
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2492 3220 3670 6987 7158
Size: 325 217 627 705 728 450 3317 171
Cuts at: 7158 7510 8965 618
Size: 352 1455 1842
Fragments arranged by size:
3317 1842 1455 728 705 627 450 352
325 217 171
BanII G_rGCy'C
Cuts at: 1391 2414 3237 3972 6483 7508 1391
Size: 1023 823 735 2511 1025 4072
Fragments arranged by size:
4072 2511 1025 1023 823 735
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2545 2597 3170 4038 4061 5328 928
Size: 971 646 52 573 868 23 1267 5789
Fragments arranged by size:
5789 1267 971 868 646 573 52 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2654 2688 5353 5387 8380 8414 1903
Size: 34 717 34 2665 34 2993 34 3678
Fragments arranged by size:
3678 2993 2665 717 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8081 9291 8081
Size: 1210 8979
BclI T'GATC_A
Cuts at: 1548 7240 1548
Size: 5692 4497
BfrBI ATG'CAT
Cuts at: 2060 6853 2060
Size: 4793 5396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8800 136
Size: 107 122 71 631 605 7128 1525
Fragments arranged by size:
7128 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5509 10184 5509
Size: 4675 5514
BlpI GC'TnA_GC
Cuts at: 5484 7606 5484
Size: 2122 8067
Bme1580I G_kGCm'C
Cuts at: 1790 2565 3673 6063 8250 9406 1790
Size: 775 1108 2390 2187 1156 2573
Fragments arranged by size:
2573 2390 2187 1156 1108 775
BmgBI CAC'GTC
Cuts at: 1318 6718 1318
Size: 5400 4789
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2825 3844 5140
Size: 416 53 83 399 24 1408 1019 1296
Cuts at: 5140 5854 6732 7322 7920 8878 442
Size: 714 878 590 598 958 1753
Fragments arranged by size:
1753 1408 1296 1019 958 878 714 598
590 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3491 1056
Size: 2435 7754
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 10189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2772 2856 5483 5999 6610 6745 8849 1239
Size: 1533 84 2627 516 611 135 2104 2579
Fragments arranged by size:
2627 2579 2104 1533 611 516 135 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6669 8314 9179 9477 6669
Size: 1645 865 298 7381
Fragments arranged by size:
7381 1645 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3388 5450 6528 6687 7119 7428 8852
Size: 617 1889 2062 1078 159 432 309 1424
Cuts at: 8852 882
Size: 2219
Fragments arranged by size:
2219 2062 1889 1424 1078 617 432 309
159
BsaAI yAC'GTr
Cuts at: 493 1204 5477 7980 493
Size: 711 4273 2503 2702
Fragments arranged by size:
4273 2702 2503 711
BsaBI GATnn'nnATC
Cuts at: 6390 10183 6390
Size: 3793 6396
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5359 7996 8378 9013
Size: 53 83 186 936 3826 2637 382 635
Cuts at: 9013 275
Size: 1451
Fragments arranged by size:
3826 2637 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4098 5642 6537 7061 8622 9020 9294 9441 9852
Size: 1544 895 524 1561 398 274 147 411
Cuts at: 9852 4098
Size: 4435
Fragments arranged by size:
4435 1561 1544 895 524 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2745 2775 5637 5667 1178
Size: 30 165 30 1342 30 2862 30 5700
Fragments arranged by size:
5700 2862 1342 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 4784 4787 5186 5380 5699 6089 6607
Size: 862 2736 3 399 194 319 390 518
Cuts at: 6607 6710 6930 7195 7380 1186
Size: 103 220 265 185 3995
Fragments arranged by size:
3995 2736 862 518 399 390 319 265
220 194 185 103 3
BseYI C'CCAG_C
Cuts at: 2229 3282 3820 5815 9392 9888 2229
Size: 1053 538 1995 3577 496 2530
Fragments arranged by size:
3577 2530 1995 1053 538 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2520 3968 5751 1163
Size: 573 784 1448 1783 5601
Fragments arranged by size:
5601 1783 1448 784 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7639 8400 9428 1540
Size: 275 186 5638 761 1028 2301
Fragments arranged by size:
5638 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3972 6579 8250 8335 9406 3972
Size: 2607 1671 85 1071 4755
Fragments arranged by size:
4755 2607 1671 1071 85
BsmI GAATG_Cn'
Cuts at: 3380 6106 7700 7793 3380
Size: 2726 1594 93 5776
Fragments arranged by size:
5776 2726 1594 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2414 2565 3237 3673 3972 6063 6483
Size: 399 624 151 672 436 299 2091 420
Cuts at: 6483 6579 7508 8250 8335 9406 1391
Size: 96 929 742 85 1071 2174
Fragments arranged by size:
2174 2091 1071 929 742 672 624 436
420 399 299 151 96 85
BspEI T'CCGG_A
Cuts at: 4098 5642 7061 9852 4098
Size: 1544 1419 2791 4435
Fragments arranged by size:
4435 2791 1544 1419
BspHI T'CATG_A
Cuts at: 7165 8078 9808 7165
Size: 913 1730 7546
Fragments arranged by size:
7546 1730 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 3433 4720 7544 844
Size: 1363 1226 1287 2824 3489
Fragments arranged by size:
3489 2824 1363 1287 1226
BsrBI CCG'CTC
Cuts at: 1394 1823 2681 5182 5945 7293 8076 1394
Size: 429 858 2501 763 1348 783 3507
Fragments arranged by size:
3507 2501 1348 858 783 763 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2062 5562 7340 8684 8858 65
Size: 1183 814 3500 1778 1344 174 1396
Fragments arranged by size:
3500 1778 1396 1344 1183 814 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3217 5896 8833 9020 1700
Size: 110 1407 2679 2937 187 2869
Fragments arranged by size:
2937 2869 2679 1407 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10189
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10189
BssSI C'ACGA_G
Cuts at: 2571 5384 8249 9261 2571
Size: 2813 2865 1012 3499
Fragments arranged by size:
3499 2865 2813 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4296 5480 794
Size: 3502 1184 5503
Fragments arranged by size:
5503 3502 1184
BstBI TT'CG_AA
Cuts at: 8993 8993
Size: 10189
BstEII G'GTnAC_C
Cuts at: 1184 3701 1184
Size: 2517 7672
BstXI CCAn_nnnn'nTGG
Cuts at: 3602 7028 3602
Size: 3426 6763
BstYI r'GATC_y
Cuts at: 1972 3795 4936 5294 5409 5509 7184 7360 7519
Size: 1823 1141 358 115 100 1675 176 159
Cuts at: 7519 7886 8271 8288 9740 9826 9838 10184 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1823 1675 1452 1141 385 367 358
346 176 159 115 100 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4974 5919 6754 4974
Size: 945 835 8409
Fragments arranged by size:
8409 945 835
BtgI C'CryG_G
Cuts at: 513 1065 4036 6838 6971 7264 513
Size: 552 2971 2802 133 293 3438
Fragments arranged by size:
3438 2971 2802 552 293 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2346 487
Size: 683 56 1120 8330
Fragments arranged by size:
8330 1120 683 56
BtsI GCAGTG_nn'
Cuts at: 4294 4378 7123 7705 8499 8519 4294
Size: 84 2745 582 794 20 5964
Fragments arranged by size:
5964 2745 794 582 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6931 6966 529
Size: 35 6367 35 3752
Fragments arranged by size:
6367 3752 35 35
DraI TTT'AAA
Cuts at: 3414 7493 3414
Size: 4079 6110
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4649 9196 809
Size: 507 3333 4547 1802
Fragments arranged by size:
4547 3333 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4843 7267 7636
Size: 54 1425 50 275 18 3013 2424 369
Cuts at: 7636 8525 10154 8
Size: 889 1629 43
Fragments arranged by size:
3013 2424 1629 1425 889 369 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7636 1537
Size: 275 5824 4090
Fragments arranged by size:
5824 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 2416 2671 3954 4896 4946 5097 5106
Size: 1023 40 255 1283 942 50 151 9
Cuts at: 5106 5172 5565 5676 6003 6120 6687 8119 1353
Size: 66 393 111 327 117 567 1432 3423
Fragments arranged by size:
3423 1432 1283 1023 942 567 393 327
255 151 117 111 66 50 40 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8776 9150 9296 387
Size: 1064 138 7187 374 146 1280
Fragments arranged by size:
7187 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3970 3970
Size: 10189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2772 2856 3096 3099 3407 3935
Size: 393 468 672 84 240 3 308 528
Cuts at: 3935 4160 4338 4620 5483 5534 5745 5999 6610
Size: 225 178 282 863 51 211 254 611
Cuts at: 6610 6745 6883 8252 8849 9636 9904 1239
Size: 135 138 1369 597 787 268 1524
Fragments arranged by size:
1524 1369 863 787 672 611 597 528
468 393 308 282 268 254 240 225
211 178 138 135 84 51 3
EcoNI CCTnn'n_nnAGG
Cuts at: 5387 5387
Size: 10189
EcoO109I rG'GnC_Cy
Cuts at: 3004 3382 3942 5380 6332 7274 3004
Size: 378 560 1438 952 942 5919
Fragments arranged by size:
5919 1438 952 942 560 378
EcoRI G'AATT_C
Cuts at: 3955 5220 7498 7876 3955
Size: 1265 2278 378 6268
Fragments arranged by size:
6268 2278 1265 378
EcoRV GAT'ATC
Cuts at: 4468 5640 6780 4468
Size: 1172 1140 7877
Fragments arranged by size:
7877 1172 1140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6913 6913
Size: 10189
FspI TGC'GCA
Cuts at: 8695 8695
Size: 10189
HaeII r_GCGC'y
Cuts at: 1678 5521 9336 1678
Size: 3843 3815 2531
Fragments arranged by size:
3843 3815 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2195 2209 2227 3899
Size: 32 135 32 605 18 14 18 1672
Cuts at: 3899 3931 3959 3991 4422 4454 5246 5258 5278
Size: 32 28 32 431 32 792 12 20
Cuts at: 5278 5290 6080 6104 6112 6136 6287 6319 7352
Size: 12 790 24 8 24 151 32 1033
Cuts at: 7352 7384 7388 7406 7420 7438 8830 8862 8904
Size: 32 4 18 14 18 1392 32 42
Cuts at: 8904 8936 1373
Size: 32 2626
Fragments arranged by size:
2626 1672 1392 1033 792 790 605 431
151 135 42 32 32 32 32 32
32 32 32 32 28 24 24 20
18 18 18 18 14 14 12 12
8 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4404 5034 7533 7779 669
Size: 988 281 2466 630 2499 246 3079
Fragments arranged by size:
3079 2499 2466 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 2085 748
Size: 1337 8852
HpaI GTT'AAC
Cuts at: 7779 7779
Size: 10189
KpnI G_GTAC'C
Cuts at: 7514 7514
Size: 10189
MfeI C'AATT_G
Cuts at: 3094 7788 3094
Size: 4694 5495
MluI A'CGCG_T
Cuts at: 10065 10065
Size: 10189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2175 3191 3942 4443 4902 5651 5698
Size: 789 81 1016 751 501 459 749 47
Cuts at: 5698 7252 9303 9487 1305
Size: 1554 2051 184 2007
Fragments arranged by size:
2051 2007 1554 1016 789 751 749 501
459 184 81 47
MscI TGG'CCA
Cuts at: 10 64 1489 1832 7269 10
Size: 54 1425 343 5437 2930
Fragments arranged by size:
5437 2930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 2744 4563 5775 5835 6996 7026
Size: 648 889 689 1819 1212 60 1161 30
Cuts at: 7026 7047 7164 8147 8506 8665 518
Size: 21 117 983 359 159 2042
Fragments arranged by size:
2042 1819 1212 1161 983 889 689 648
359 159 117 60 30 21
MspA1I CmG'CkG
Cuts at: 1606 2437 3598 4640 5544 5578 6499 7605 8282
Size: 831 1161 1042 904 34 921 1106 677
Cuts at: 8282 9430 9675 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1161 1148 1106 1042 921 904 831
677 245 34
NaeI GCC'GGC
Cuts at: 3219 3219
Size: 10189
NcoI C'CATG_G
Cuts at: 513 1065 4036 6838 6971 7264 513
Size: 552 2971 2802 133 293 3438
Fragments arranged by size:
3438 2971 2802 552 293 133
NdeI CA'TA_TG
Cuts at: 387 3790 6416 7044 387
Size: 3403 2626 628 3532
Fragments arranged by size:
3532 3403 2626 628
NgoMIV G'CCGG_C
Cuts at: 3217 3217
Size: 10189
NheI G'CTAG_C
Cuts at: 1052 3487 1052
Size: 2435 7754
NotI GC'GGCC_GC
Cuts at: 7636 7636
Size: 10189
NruI TCG'CGA
Cuts at: 2365 2365
Size: 10189
NsiI A_TGCA'T
Cuts at: 2062 6855 2062
Size: 4793 5396
NspI r_CATG'y
Cuts at: 1132 1229 7087 7547 9092 1132
Size: 97 5858 460 1545 2229
Fragments arranged by size:
5858 2229 1545 460 97
PacI TTA_AT'TAA
Cuts at: 5856 5856
Size: 10189
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10189
PciI A'CATG_T
Cuts at: 7083 9088 7083
Size: 2005 8184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2144 4513 1108
Size: 1036 2369 6784
Fragments arranged by size:
6784 2369 1036
PfoI T'CCnGG_A
Cuts at: 2137 4854 2137
Size: 2717 7472
PmeI GTTT'AAAC
Cuts at: 7493 7493
Size: 10189
PmlI CAC'GTG
Cuts at: 5477 5477
Size: 10189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2156 2188 4137 4169 8258 8290 9771 9803 2156
Size: 32 1949 32 4089 32 1481 32 2542
Fragments arranged by size:
4089 2542 1949 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3942 5380 6332 7274 3942
Size: 1438 952 942 6857
Fragments arranged by size:
6857 1438 952 942
PshAI GACnn'nnGTC
Cuts at: 1613 7234 1613
Size: 5621 4568
PsiI TTA'TAA
Cuts at: 3782 5433 7467 7759 3782
Size: 1651 2034 292 6212
Fragments arranged by size:
6212 2034 1651 292
PstI C_TGCA'G
Cuts at: 830 7354 7541 830
Size: 6524 187 3478
Fragments arranged by size:
6524 3478 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10189
PvuII CAG'CTG
Cuts at: 2437 3598 5544 5578 6499 2437
Size: 1161 1946 34 921 6127
Fragments arranged by size:
6127 1946 1161 921 34
SacI G_AGCT'C
Cuts at: 3972 3972
Size: 10189
SalI G'TCGA_C
Cuts at: 1936 7531 1936
Size: 5595 4594
SanDI GG'GwC_CC
Cuts at: 3942 7274 3942
Size: 3332 6857
SapI GCTCTTCn'nnn_
Cuts at: 2671 4896 5097 5106 5172 5565 2671
Size: 2225 201 9 66 393 7295
Fragments arranged by size:
7295 2225 393 201 66 9
SbfI CC_TGCA'GG
Cuts at: 7541 7541
Size: 10189
ScaI AGT'ACT
Cuts at: 8437 10149 8437
Size: 1712 8477
SfcI C'TryA_G
Cuts at: 826 1045 5933 7350 7537 8672 9353 9544 826
Size: 219 4888 1417 187 1135 681 191 1471
Fragments arranged by size:
4888 1471 1417 1135 681 219 191 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10189
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10189
SmaI CCC'GGG
Cuts at: 1872 7516 1872
Size: 5644 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6684 8293 9194 9456 820
Size: 197 926 4741 1609 901 262 1553
Fragments arranged by size:
4741 1609 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7980 493
Size: 7487 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10189
SphI G_CATG'C
Cuts at: 1132 7547 1132
Size: 6415 3774
SspI AAT'ATT
Cuts at: 5 52 2374 3089 4264 4738 8113 5
Size: 47 2322 715 1175 474 3375 2081
Fragments arranged by size:
3375 2322 2081 1175 715 474 47
StuI AGG'CCT
Cuts at: 1517 6752 7099 7201 1517
Size: 5235 347 102 4505
Fragments arranged by size:
5235 4505 347 102
StyI C'CwwG_G
Cuts at: 513 1065 3541 4036 6101 6838 6971 7264 7628
Size: 552 2476 495 2065 737 133 293 364
Cuts at: 7628 513
Size: 3074
Fragments arranged by size:
3074 2476 2065 737 552 495 364 293
133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5513 6876 7137 8216 8233 8386 8571
Size: 1255 3933 1363 261 1079 17 153 185
Cuts at: 8571 9016 325
Size: 445 1498
Fragments arranged by size:
3933 1498 1363 1255 1079 445 261 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7110 8435 10147
Size: 274 80 33 51 1443 5133 1325 1712
Cuts at: 10147 96
Size: 138
Fragments arranged by size:
5133 1712 1443 1325 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10149 10181 10149
Size: 32 10157
Tth111I GACn'n_nGTC
Cuts at: 1316 4646 1316
Size: 3330 6859
XbaI T'CTAG_A
Cuts at: 7525 7525
Size: 10189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4514 4798 7408 7467 4514
Size: 284 2610 59 7236
Fragments arranged by size:
7236 2610 284 59
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10189
XmaI C'CCGG_G
Cuts at: 1870 7514 1870
Size: 5644 4545
XmnI GAAnn'nnTTC
Cuts at: 3741 4149 8318 3741
Size: 408 4169 5612
Fragments arranged by size:
5612 4169 408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7997 276
Size: 53 83 186 936 6463 2468
Fragments arranged by size:
6463 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NarI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI