Restriction map for
pFN21AE9636
(Circular) (Six-base) MAPSORT of: pFN21AE9636.seq Check: 3966 from: 1 to: 9382
pFN21AE9636 9382 bp
With 182 enzymes: SgfI *
March 28, 2012 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4762 5055 5566 4762
Size: 293 511 8578
Fragments arranged by size:
8578 511 293
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7192 278
Size: 53 83 186 936 5656 2468
Fragments arranged by size:
5656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4039 4253 4537 6725 1937
Size: 2102 214 284 2188 4594
Fragments arranged by size:
4594 2188 2102 284 214
Acc65I G'GTAC_C
Cuts at: 6703 6703
Size: 9382
AclI AA'CG_TT
Cuts at: 1563 7509 7882 1563
Size: 5946 373 3063
Fragments arranged by size:
5946 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 4310 4419 5916 6585 7445 8829 9097
Size: 544 2134 109 1497 669 860 1384 268
Cuts at: 9097 1632
Size: 1917
Fragments arranged by size:
2134 1917 1497 1384 860 669 544 268
109
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9185
AflIII A'CryG_T
Cuts at: 1203 2534 4139 8281 9258 1203
Size: 1331 1605 4142 977 1327
Fragments arranged by size:
4142 1605 1331 1327 977
AgeI A'CCGG_T
Cuts at: 8213 8213
Size: 9382
AhdI GACnn_n'nnGTC
Cuts at: 4862 8111 4862
Size: 3249 6133
AleI CACnn'nnGTG
Cuts at: 1166 2041 2813 3585 1166
Size: 875 772 772 6963
Fragments arranged by size:
6963 875 772 772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3512 4489 6047 1184
Size: 2328 977 1558 4519
Fragments arranged by size:
4519 2328 1558 977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 9350
AlwNI CAG_nnn'CTG
Cuts at: 2218 4076 4240 4711 4927 5227 5435 5581 5803
Size: 1858 164 471 216 300 208 146 222
Cuts at: 5803 5887 5993 6268 6467 7125 8697 2218
Size: 84 106 275 199 658 1572 2903
Fragments arranged by size:
2903 1858 1572 658 471 300 275 222
216 208 199 164 146 106 84
ApaI G_GGCC'C
Cuts at: 6193 6364 6521 6193
Size: 171 157 9054
Fragments arranged by size:
9054 171 157
ApaLI G'TGCA_C
Cuts at: 7439 8595 7439
Size: 1156 8226
ApoI r'AATT_y
Cuts at: 1493 2276 3344 5278 5723 6691 6917 7069 9178
Size: 783 1068 1934 445 968 226 152 2109
Cuts at: 9178 1493
Size: 1697
Fragments arranged by size:
2109 1934 1697 1068 968 783 445 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7936 160
Size: 872 6904 1606
Fragments arranged by size:
6904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9382
AvaI C'yCGr_G
Cuts at: 1870 1943 2409 2994 3168 4482 5088 5819 6186
Size: 73 466 585 174 1314 606 731 367
Cuts at: 6186 6227 6351 6420 6707 1870
Size: 41 124 69 287 4545
Fragments arranged by size:
4545 1314 731 606 585 466 367 287
174 124 73 69 41
BamHI G'GATC_C
Cuts at: 6300 6712 7079 6300
Size: 412 367 8603
Fragments arranged by size:
8603 412 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3102 3247 3381 3496 3610
Size: 325 217 627 1315 145 134 115 114
Cuts at: 3610 3879 4744 4835 4871 5754 6703 8158 618
Size: 269 865 91 36 883 949 1455 1842
Fragments arranged by size:
1842 1455 1315 949 883 865 627 325
269 217 145 134 115 114 91 36
BanII G_rGCy'C
Cuts at: 1391 2297 2410 4489 4560 5599 5934 6107 6193
Size: 906 113 2079 71 1039 335 173 86
Cuts at: 6193 6207 6352 6364 6455 6521 6701 1391
Size: 14 145 12 91 66 180 4072
Fragments arranged by size:
4072 2079 1039 906 335 180 173 145
113 91 86 71 66 14 12
BbeI G_GCGC'C
Cuts at: 3883 5758 3883
Size: 1875 7507
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2682 3514 3602 3916 928
Size: 971 783 832 88 314 6394
Fragments arranged by size:
6394 971 832 783 314 88
BbvCI CC'TCA_GC
Cuts at: 4656 4822 5660 6468 6669 4656
Size: 166 838 808 201 7369
Fragments arranged by size:
7369 838 808 201 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2106 2140 5477 5511 7573 7607 1903
Size: 34 169 34 3337 34 2062 34 3678
Fragments arranged by size:
3678 3337 2062 169 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5492 7274 8484 5492
Size: 1782 1210 6390
Fragments arranged by size:
6390 1782 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9382
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3877 4179 4652
Size: 107 122 71 631 605 2205 302 473
Cuts at: 4652 5812 5882 7993 136
Size: 1160 70 2111 1525
Fragments arranged by size:
2205 2111 1525 1160 631 605 473 302
122 107 71 70
BglII A'GATC_T
Cuts at: 2712 6118 9377 2712
Size: 3406 3259 2717
Fragments arranged by size:
3406 3259 2717
BlpI GC'TnA_GC
Cuts at: 4077 5928 6799 4077
Size: 1851 871 6660
Fragments arranged by size:
6660 1851 871
Bme1580I G_kGCm'C
Cuts at: 1790 2048 3107 3252 3501 3615 4390 4838 6193
Size: 258 1059 145 249 114 775 448 1355
Cuts at: 6193 6364 6521 7443 8599 1790
Size: 171 157 922 1156 2573
Fragments arranged by size:
2573 1355 1156 1059 922 775 448 258
249 171 157 145 114
BmgBI CAC'GTC
Cuts at: 1318 2537 1318
Size: 1219 8163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2953 3592 5511
Size: 416 53 83 399 24 1536 639 1919
Cuts at: 5511 6516 7113 8071 442
Size: 1005 597 958 1753
Fragments arranged by size:
1919 1753 1536 1005 958 639 597 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5272 6121 6366 5272
Size: 849 245 8288
Fragments arranged by size:
8288 849 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2532 2897 3298 3577 4076 4207 4364 4672
Size: 1293 365 401 279 499 131 157 308
Cuts at: 4672 4748 4994 5216 5263 5318 5786 6074 6143
Size: 76 246 222 47 55 468 288 69
Cuts at: 6143 6314 6335 6341 6386 6515 8042 1239
Size: 171 21 6 45 129 1527 2579
Fragments arranged by size:
2579 1527 1293 499 468 401 365 308
288 279 246 222 171 157 131 129
76 69 55 47 45 21 6
Bpu10I CC'TnA_GC
Cuts at: 4656 4822 5082 5660 6106 6454 6468 6669 4656
Size: 166 260 578 446 348 14 201 7369
Fragments arranged by size:
7369 578 446 348 260 201 166 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5027 5051 5255 5734 7507 8372 8670 5027
Size: 24 204 479 1773 865 298 5739
Fragments arranged by size:
5739 1773 865 479 298 204 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2073 5762 8045 882
Size: 617 574 3689 2283 2219
Fragments arranged by size:
3689 2283 2219 617 574
BsaAI yAC'GTr
Cuts at: 493 1204 3511 4140 7173 493
Size: 711 2307 629 3033 2702
Fragments arranged by size:
3033 2702 2307 711 629
BsaBI GATnn'nnATC
Cuts at: 4013 9376 4013
Size: 5363 4019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3880 4036 5755 7189
Size: 53 83 186 936 2347 156 1719 1434
Cuts at: 7189 7571 8206 275
Size: 382 635 1451
Fragments arranged by size:
2347 1719 1451 1434 936 635 382 186
156 83 53
BsaWI w'CCGG_w
Cuts at: 2038 2659 3909 7815 8213 8487 8634 9045 2038
Size: 621 1250 3906 398 274 147 411 2375
Fragments arranged by size:
3906 2375 1250 621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 3257 3287 3765
Size: 30 165 30 597 30 1227 30 478
Cuts at: 3765 3795 5357 5387 6054 6084 6116 6146 6616
Size: 30 1562 30 667 30 32 30 470
Cuts at: 6616 6646 1178
Size: 30 3914
Fragments arranged by size:
3914 1562 1227 667 597 478 470 165
32 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2287 2567 2840 2903 3060 3413 3416 3950
Size: 1101 280 273 63 157 353 3 534
Cuts at: 3950 4967 5213 5536 5648 5981 6113 6215 6440
Size: 1017 246 323 112 333 132 102 225
Cuts at: 6440 6485 6497 6512 6515 1186
Size: 45 12 15 3 4053
Fragments arranged by size:
4053 1101 1017 534 353 333 323 280
273 246 225 157 132 112 102 63
45 15 12 3 3
BseYI C'CCAG_C
Cuts at: 2064 2418 3875 4732 4755 4947 5368 5398 5585
Size: 354 1457 857 23 192 421 30 187
Cuts at: 5585 6415 6551 8585 9081 2064
Size: 830 136 2034 496 2365
Fragments arranged by size:
2365 2034 1457 857 830 496 421 354
192 187 136 30 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 2899 3677 3789 5337 6510 1163
Size: 573 888 275 778 112 1548 1173 4035
Fragments arranged by size:
4035 1548 1173 888 778 573 275 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6832 7593 8621 1540
Size: 275 186 4831 761 1028 2301
Fragments arranged by size:
4831 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3897 4223 4489 4560 5540 5599 5934 6207 6455
Size: 326 266 71 980 59 335 273 248
Cuts at: 6455 7443 7528 8599 3897
Size: 988 85 1071 4680
Fragments arranged by size:
4680 1071 988 980 335 326 273 266
248 85 71 59
BsmI GAATG_Cn'
Cuts at: 6893 6986 6893
Size: 93 9289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2297 2410 3107 3252 3501 3615
Size: 399 258 249 113 697 145 249 114
Cuts at: 3615 3897 4223 4390 4489 4560 4838 5540 5599
Size: 282 326 167 99 71 278 702 59
Cuts at: 5599 5934 6107 6193 6207 6352 6364 6455 6521
Size: 335 173 86 14 145 12 91 66
Cuts at: 6521 6701 7443 7528 8599 1391
Size: 180 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 697 399 335 326
282 278 258 249 249 180 173 167
145 145 114 113 99 91 86 85
71 66 59 14 12
BspEI T'CCGG_A
Cuts at: 2659 3909 9045 2659
Size: 1250 5136 2996
Fragments arranged by size:
5136 2996 1250
BspHI T'CATG_A
Cuts at: 3711 6207 7271 9001 3711
Size: 2496 1064 1730 4092
Fragments arranged by size:
4092 2496 1730 1064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4248 4596 4762 5055 5566 6231 6737 844
Size: 3404 348 166 293 511 665 506 3489
Fragments arranged by size:
3489 3404 665 511 506 348 293 166
BsrBI CCG'CTC
Cuts at: 1394 1823 4033 4411 7269 1394
Size: 429 2210 378 2858 3507
Fragments arranged by size:
3507 2858 2210 429 378
BsrDI GCAATG_nn'
Cuts at: 65 1248 2959 4935 7877 8051 65
Size: 1183 1711 1976 2942 174 1396
Fragments arranged by size:
2942 1976 1711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2631 3439 3903 4230 5324 5349 6583
Size: 110 821 808 464 327 1094 25 1234
Cuts at: 6583 8026 8213 1700
Size: 1443 187 2869
Fragments arranged by size:
2869 1443 1234 1094 821 808 464 327
187 110 25
BsrGI T'GTAC_A
Cuts at: 96 5376 96
Size: 5280 4102
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9382
BssSI C'ACGA_G
Cuts at: 4662 7442 8454 4662
Size: 2780 1012 5590
Fragments arranged by size:
5590 2780 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3247 4240 4927 5803 5993 6268 794
Size: 2453 993 687 876 190 275 3908
Fragments arranged by size:
3908 2453 993 876 687 275 190
BstBI TT'CG_AA
Cuts at: 8186 8186
Size: 9382
BstEII G'GTnAC_C
Cuts at: 1184 5126 6232 1184
Size: 3942 1106 4334
Fragments arranged by size:
4334 3942 1106
BstXI CCAn_nnnn'nTGG
Cuts at: 2737 2815 4514 5524 2737
Size: 78 1699 1010 6595
Fragments arranged by size:
6595 1699 1010 78
BstYI r'GATC_y
Cuts at: 1972 2139 2328 2712 3081 5115 6118 6300 6712
Size: 167 189 384 369 2034 1003 182 412
Cuts at: 6712 7079 7464 7481 8933 9019 9031 9377 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2034 1977 1452 1003 412 385 384 369
367 346 189 182 167 86 17 12
BstZ17I GTA'TAC
Cuts at: 4254 4254
Size: 9382
Bsu36I CC'TnA_GG
Cuts at: 4749 5420 5964 6036 6177 4749
Size: 671 544 72 141 7954
Fragments arranged by size:
7954 671 544 141 72
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 3736 5629 513
Size: 552 939 147 1585 1893 4266
Fragments arranged by size:
4266 1893 1585 939 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2080 2098 2844 4801 6423 487
Size: 683 56 854 18 746 1957 1622 3446
Fragments arranged by size:
3446 1957 1622 854 746 683 56 18
BtsI GCAGTG_nn'
Cuts at: 3855 4679 5609 5693 5780 5843 6025 6898 7692
Size: 824 930 84 87 63 182 873 794
Cuts at: 7692 7712 3855
Size: 20 5525
Fragments arranged by size:
5525 930 873 824 794 182 87 84
63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3241 3276 529
Size: 35 2677 35 6635
Fragments arranged by size:
6635 2677 35 35
DraI TTT'AAA
Cuts at: 3214 6686 3214
Size: 3472 5910
DraIII CAC_nnn'GTG
Cuts at: 4528 4528
Size: 9382
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4184 8389 809
Size: 507 2868 4205 1802
Fragments arranged by size:
4205 2868 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2629 2754 3201
Size: 54 1425 50 275 18 799 125 447
Cuts at: 3201 4504 5508 6829 7718 9347 8
Size: 1303 1004 1321 889 1629 43
Fragments arranged by size:
1629 1425 1321 1303 1004 889 799 447
275 125 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6829 1537
Size: 275 5017 4090
Fragments arranged by size:
5017 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3390 4023 6483 7312 1353
Size: 2037 633 2460 829 3423
Fragments arranged by size:
3423 2460 2037 829 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2132 2341 3137 4375 5924 6397
Size: 1064 138 543 209 796 1238 1549 473
Cuts at: 6397 6602 7969 8343 8489 387
Size: 205 1367 374 146 1280
Fragments arranged by size:
1549 1367 1280 1238 1064 796 543 473
374 209 205 146 138
EcoICRI GAG'CTC
Cuts at: 4487 4558 5597 5932 6205 6453 4487
Size: 71 1039 335 273 248 7416
Fragments arranged by size:
7416 1039 335 273 248 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2532 2897 3298 3577 4076 4207
Size: 393 544 356 365 401 279 499 131
Cuts at: 4207 4310 4364 4419 4672 4748 4994 5216 5263
Size: 103 54 55 253 76 246 222 47
Cuts at: 5263 5318 5786 5916 6074 6143 6314 6335 6341
Size: 55 468 130 158 69 171 21 6
Cuts at: 6341 6386 6515 6585 7445 8042 8829 9097 1239
Size: 45 129 70 860 597 787 268 1524
Fragments arranged by size:
1524 860 787 597 544 499 468 401
393 365 356 279 268 253 246 222
171 158 131 130 129 103 76 70
69 55 55 54 47 45 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4460 5615 6011 6227 4460
Size: 1155 396 216 7615
Fragments arranged by size:
7615 1155 396 216
EcoO109I rG'GnC_Cy
Cuts at: 2726 2792 4048 4669 5158 5179 5416 5425 5830
Size: 66 1256 621 489 21 237 9 405
Cuts at: 5830 6190 6220 6361 6517 6518 6605 2726
Size: 360 30 141 156 1 87 5503
Fragments arranged by size:
5503 1256 621 489 405 360 237 156
141 87 66 30 21 9 1
EcoRI G'AATT_C
Cuts at: 6691 7069 6691
Size: 378 9004
EcoRV GAT'ATC
Cuts at: 3481 3481
Size: 9382
FspI TGC'GCA
Cuts at: 5412 7888 5412
Size: 2476 6906
HaeII r_GCGC'y
Cuts at: 1678 2425 3883 4117 5758 8529 1678
Size: 747 1458 234 1641 2771 2531
Fragments arranged by size:
2771 2531 1641 1458 747 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2843 2875 2977
Size: 32 135 32 428 32 811 32 102
Cuts at: 2977 3009 3315 3347 3765 3797 3906 3938 4848
Size: 32 306 32 418 32 109 32 910
Cuts at: 4848 4880 5312 5344 5417 5449 5662 5694 6116
Size: 32 432 32 73 32 213 32 422
Cuts at: 6116 6148 8023 8055 8097 8129 1373
Size: 32 1875 32 42 32 2626
Fragments arranged by size:
2626 1875 910 811 432 428 422 418
306 213 135 109 102 73 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3847 6153 6726 6972 669
Size: 988 281 918 991 2306 573 246 3079
Fragments arranged by size:
3079 2306 991 988 918 573 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9382
HpaI GTT'AAC
Cuts at: 6972 6972
Size: 9382
KasI G'GCGC_C
Cuts at: 3879 5754 3879
Size: 1875 7507
KpnI G_GTAC'C
Cuts at: 6707 6707
Size: 9382
MfeI C'AATT_G
Cuts at: 6981 6981
Size: 9382
MluI A'CGCG_T
Cuts at: 9258 9258
Size: 9382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2043 3401 3426 4695 6147 8496 8680 1305
Size: 738 1358 25 1269 1452 2349 184 2007
Fragments arranged by size:
2349 2007 1452 1358 1269 738 184 25
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 10
Size: 54 1425 343 924 6636
Fragments arranged by size:
6636 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2041 2813 2921 2952 3236 3471 3494
Size: 648 875 772 108 31 284 235 23
Cuts at: 3494 3585 3710 3728 4019 4203 7340 7699 7858
Size: 91 125 18 291 184 3137 359 159
Cuts at: 7858 518
Size: 2042
Fragments arranged by size:
3137 2042 875 772 648 359 291 284
235 184 159 125 108 91 31 23
18
MspA1I CmG'CkG
Cuts at: 1606 2153 3354 3810 3995 4503 4732 4768 5491
Size: 547 1201 456 185 508 229 36 723
Cuts at: 5491 5862 6265 6798 7475 8623 8868 1606
Size: 371 403 533 677 1148 245 2120
Fragments arranged by size:
2120 1201 1148 723 677 547 533 508
456 403 371 245 229 185 36
NaeI GCC'GGC
Cuts at: 2633 3905 4232 5326 2633
Size: 1272 327 1094 6689
Fragments arranged by size:
6689 1272 1094 327
NarI GG'CG_CC
Cuts at: 3880 5755 3880
Size: 1875 7507
NcoI C'CATG_G
Cuts at: 513 1065 2004 3736 5629 513
Size: 552 939 1732 1893 4266
Fragments arranged by size:
4266 1893 1732 939 552
NdeI CA'TA_TG
Cuts at: 387 2974 3243 6257 387
Size: 2587 269 3014 3512
Fragments arranged by size:
3512 3014 2587 269
NgoMIV G'CCGG_C
Cuts at: 2631 3903 4230 5324 2631
Size: 1272 327 1094 6689
Fragments arranged by size:
6689 1272 1094 327
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9382
NotI GC'GGCC_GC
Cuts at: 6829 6829
Size: 9382
NruI TCG'CGA
Cuts at: 2099 2099
Size: 9382
NspI r_CATG'y
Cuts at: 1132 1229 3235 6740 8285 1132
Size: 97 2006 3505 1545 2229
Fragments arranged by size:
3505 2229 2006 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9382
PciI A'CATG_T
Cuts at: 8281 8281
Size: 9382
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2280 3420 4528 5862 1108
Size: 1172 1140 1108 1334 4628
Fragments arranged by size:
4628 1334 1172 1140 1108
PfoI T'CCnGG_A
Cuts at: 2874 5240 6477 2874
Size: 2366 1237 5779
Fragments arranged by size:
5779 2366 1237
PmeI GTTT'AAAC
Cuts at: 6686 6686
Size: 9382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 7451 7483 8964 8996 3257
Size: 32 4162 32 1481 32 3643
Fragments arranged by size:
4162 3643 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5179 5416 5425 6220 5179
Size: 237 9 795 8341
Fragments arranged by size:
8341 795 237 9
PshAI GACnn'nnGTC
Cuts at: 1613 5613 1613
Size: 4000 5382
PsiI TTA'TAA
Cuts at: 6952 6952
Size: 9382
PspOMI G'GGCC_C
Cuts at: 6189 6360 6517 6189
Size: 171 157 9054
Fragments arranged by size:
9054 171 157
PspXI vC'TCGA_Gb
Cuts at: 6227 6227
Size: 9382
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3441 3473 3441
Size: 32 9350
PstI C_TGCA'G
Cuts at: 830 2652 3186 4440 4707 4923 5490 5604 5799
Size: 1822 534 1254 267 216 567 114 195
Cuts at: 5799 5838 5989 6273 6734 830
Size: 39 151 284 461 3478
Fragments arranged by size:
3478 1822 1254 567 534 461 284 267
216 195 151 114 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9382
PvuII CAG'CTG
Cuts at: 3995 4732 5491 5862 6265 3995
Size: 737 759 371 403 7112
Fragments arranged by size:
7112 759 737 403 371
SacI G_AGCT'C
Cuts at: 4489 4560 5599 5934 6207 6455 4489
Size: 71 1039 335 273 248 7416
Fragments arranged by size:
7416 1039 335 273 248 71
SacII CC_GC'GG
Cuts at: 2154 2154
Size: 9382
SalI G'TCGA_C
Cuts at: 1936 6724 1936
Size: 4788 4594
SanDI GG'GwC_CC
Cuts at: 5425 5425
Size: 9382
SapI GCTCTTCn'nnn_
Cuts at: 4023 6483 4023
Size: 2460 6922
SbfI CC_TGCA'GG
Cuts at: 6734 6734
Size: 9382
ScaI AGT'ACT
Cuts at: 7630 9342 7630
Size: 1712 7670
SexAI A'CCwGG_T
Cuts at: 3599 3599
Size: 9382
SfcI C'TryA_G
Cuts at: 826 1045 2341 2648 2708 3182 4436 4538 4703
Size: 219 1296 307 60 474 1254 102 165
Cuts at: 4703 4919 5219 5486 5600 5795 5834 5945 5985
Size: 216 300 267 114 195 39 111 40
Cuts at: 5985 6269 6730 7865 8546 8737 826
Size: 284 461 1135 681 191 1471
Fragments arranged by size:
1471 1296 1254 1135 681 474 461 307
300 284 267 219 216 195 191 165
114 111 102 60 40 39
SfoI GGC'GCC
Cuts at: 3881 5756 3881
Size: 1875 7507
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9382
SmaI CCC'GGG
Cuts at: 1872 3170 5090 6188 6709 1872
Size: 1298 1920 1098 521 4545
Fragments arranged by size:
4545 1920 1298 1098 521
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2994 5006 5066 5270 5749 6227
Size: 197 926 1051 2012 60 204 479 478
Cuts at: 6227 7486 8387 8649 820
Size: 1259 901 262 1553
Fragments arranged by size:
2012 1553 1259 1051 926 901 479 478
262 204 197 60
SnaBI TAC'GTA
Cuts at: 493 7173 493
Size: 6680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9382
SphI G_CATG'C
Cuts at: 1132 6740 1132
Size: 5608 3774
SrfI GCCC'GGGC
Cuts at: 5090 5090
Size: 9382
SspI AAT'ATT
Cuts at: 5 52 2449 7306 5
Size: 47 2397 4857 2081
Fragments arranged by size:
4857 2397 2081 47
StuI AGG'CCT
Cuts at: 1517 4646 5255 1517
Size: 3129 609 5644
Fragments arranged by size:
5644 3129 609
StyI C'CwwG_G
Cuts at: 513 1065 2004 3087 3736 3820 4257 4839 5629
Size: 552 939 1083 649 84 437 582 790
Cuts at: 5629 6195 6821 513
Size: 566 626 3074
Fragments arranged by size:
3074 1083 939 790 649 626 582 566
552 437 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6544 6572 7409 7426 7579 7764 8209
Size: 1255 4964 28 837 17 153 185 445
Cuts at: 8209 325
Size: 1498
Fragments arranged by size:
4964 1498 1255 837 445 185 153 28
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3457 5376 7628
Size: 274 80 33 51 1443 1480 1919 2252
Cuts at: 7628 9340 96
Size: 1712 138
Fragments arranged by size:
2252 1919 1712 1480 1443 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 6054 6086 6616 6648 9342 9374 2387
Size: 32 3635 32 530 32 2694 32 2395
Fragments arranged by size:
3635 2694 2395 530 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9382
XbaI T'CTAG_A
Cuts at: 6718 6718
Size: 9382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2154 3259 4529 5700 5884 2154
Size: 1105 1270 1171 184 5652
Fragments arranged by size:
5652 1270 1171 1105 184
XhoI C'TCGA_G
Cuts at: 1943 2994 6227 1943
Size: 1051 3233 5098
Fragments arranged by size:
5098 3233 1051
XmaI C'CCGG_G
Cuts at: 1870 3168 5088 6186 6707 1870
Size: 1298 1920 1098 521 4545
Fragments arranged by size:
4545 1920 1298 1098 521
XmnI GAAnn'nnTTC
Cuts at: 3272 7511 3272
Size: 4239 5143
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7190 276
Size: 53 83 186 936 5656 2468
Fragments arranged by size:
5656 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI FalI
FseI FspAI NsiI PacI PmlI RsrII SfiI SwaI