Restriction map for
pFN21AE9629
(Circular) (Six-base) MAPSORT of: pFN21AE9629.seq Check: 3456 from: 1 to: 7708
pFN21AE9629 7708 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2509 2509
Size: 7708
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5518 278
Size: 53 83 186 936 3982 2468
Fragments arranged by size:
3982 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2385 5051 1937
Size: 448 2666 4594
Fragments arranged by size:
4594 2666 448
Acc65I G'GTAC_C
Cuts at: 5029 5029
Size: 7708
AclI AA'CG_TT
Cuts at: 1563 5835 6208 1563
Size: 4272 373 3063
Fragments arranged by size:
4272 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2666 3800 5771 7155 7423 1632
Size: 618 416 1134 1971 1384 268 1917
Fragments arranged by size:
1971 1917 1384 1134 618 416 268
AflII C'TTAA_G
Cuts at: 820 1017 4004 820
Size: 197 2987 4524
Fragments arranged by size:
4524 2987 197
AflIII A'CryG_T
Cuts at: 1203 2490 4766 6607 7584 1203
Size: 1287 2276 1841 977 1327
Fragments arranged by size:
2276 1841 1327 1287 977
AgeI A'CCGG_T
Cuts at: 6539 6539
Size: 7708
AhdI GACnn_n'nnGTC
Cuts at: 2161 4927 6437 2161
Size: 2766 1510 3432
Fragments arranged by size:
3432 2766 1510
AleI CACnn'nnGTG
Cuts at: 1166 3462 4647 4767 1166
Size: 2296 1185 120 4107
Fragments arranged by size:
4107 2296 1185 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7708
AlwNI CAG_nnn'CTG
Cuts at: 2273 2431 2632 2674 3301 4318 5451 7023 2273
Size: 158 201 42 627 1017 1133 1572 2958
Fragments arranged by size:
2958 1572 1133 1017 627 201 158 42
ApaI G_GGCC'C
Cuts at: 4271 4490 4271
Size: 219 7489
ApaLI G'TGCA_C
Cuts at: 5765 6921 5765
Size: 1156 6552
ApoI r'AATT_y
Cuts at: 1493 2201 2219 2377 2849 3809 5017 5243 5395
Size: 708 18 158 472 960 1208 226 152
Cuts at: 5395 7504 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1208 960 708 472 226 158
152 18
AseI AT'TA_AT
Cuts at: 160 1032 6262 160
Size: 872 5230 1606
Fragments arranged by size:
5230 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7708
AvaI C'yCGr_G
Cuts at: 1870 1943 4905 5033 1870
Size: 73 2962 128 4545
Fragments arranged by size:
4545 2962 128 73
AvrII C'CTAG_G
Cuts at: 4645 4645
Size: 7708
BamHI G'GATC_C
Cuts at: 2920 3494 4638 5038 5405 2920
Size: 574 1144 400 367 5223
Fragments arranged by size:
5223 1144 574 400 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3690 4607 4752 5029 6484
Size: 325 217 627 1903 917 145 277 1455
Cuts at: 6484 618
Size: 1842
Fragments arranged by size:
1903 1842 1455 917 627 325 277 217
145
BanII G_rGCy'C
Cuts at: 1391 2533 3966 4271 4490 4846 5027 1391
Size: 1142 1433 305 219 356 181 4072
Fragments arranged by size:
4072 1433 1142 356 305 219 181
BbeI G_GCGC'C
Cuts at: 4611 4756 4611
Size: 145 7563
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 928
Size: 971 162 6575
Fragments arranged by size:
6575 971 162
BbvCI CC'TCA_GC
Cuts at: 4034 4034
Size: 7708
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2042 2076 3557 3591 5899 5933 1903
Size: 34 105 34 1481 34 2308 34 3678
Fragments arranged by size:
3678 2308 1481 105 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3456 5600 6810 3456
Size: 2144 1210 4354
Fragments arranged by size:
4354 2144 1210
BclI T'GATC_A
Cuts at: 1548 2593 3454 1548
Size: 1045 861 5802
Fragments arranged by size:
5802 1045 861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2783 4629 6319
Size: 107 122 71 631 605 1111 1846 1690
Cuts at: 6319 136
Size: 1525
Fragments arranged by size:
1846 1690 1525 1111 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2211 2948 7703 2211
Size: 737 4755 2216
Fragments arranged by size:
4755 2216 737
BlpI GC'TnA_GC
Cuts at: 4063 5125 4063
Size: 1062 6646
Bme1580I G_kGCm'C
Cuts at: 1790 3693 3980 4271 4490 5769 6925 1790
Size: 1903 287 291 219 1279 1156 2573
Fragments arranged by size:
2573 1903 1279 1156 291 287 219
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7708
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2759 3895 4380
Size: 416 53 83 399 24 1342 1136 485
Cuts at: 4380 5439 6397 442
Size: 1059 958 1753
Fragments arranged by size:
1753 1342 1136 1059 958 485 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4284 1056
Size: 3228 4480
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4078 4078
Size: 7708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3481 4112 4217 4812 4860 4898 6368 1239
Size: 2242 631 105 595 48 38 1470 2579
Fragments arranged by size:
2579 2242 1470 631 595 105 48 38
Bpu10I CC'TnA_GC
Cuts at: 3351 3387 4034 3351
Size: 36 647 7025
Fragments arranged by size:
7025 647 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2961 3020 3057 5833 6698 6996 2961
Size: 59 37 2776 865 298 3673
Fragments arranged by size:
3673 2776 865 298 59 37
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3356 3530 3626 6371 882
Size: 617 1857 174 96 2745 2219
Fragments arranged by size:
2745 2219 1857 617 174 96
BsaAI yAC'GTr
Cuts at: 493 1204 2491 3655 5499 493
Size: 711 1287 1164 1844 2702
Fragments arranged by size:
2702 1844 1287 1164 711
BsaBI GATnn'nnATC
Cuts at: 2229 3380 3444 7702 2229
Size: 1151 64 4258 2235
Fragments arranged by size:
4258 2235 1151 64
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4608 4753 5515 5897
Size: 53 83 186 936 3075 145 762 382
Cuts at: 5897 6532 275
Size: 635 1451
Fragments arranged by size:
3075 1451 936 762 635 382 186 145
83 53
BsaWI w'CCGG_w
Cuts at: 2034 6141 6539 6813 6960 7371 2034
Size: 4107 398 274 147 411 2371
Fragments arranged by size:
4107 2371 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7483
Fragments arranged by size:
7483 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7708
BseYI C'CCAG_C
Cuts at: 3168 3509 3930 3949 4347 6911 7407 3168
Size: 341 421 19 398 2564 496 3469
Fragments arranged by size:
3469 2564 496 421 398 341 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3854 4748 1163
Size: 573 2118 894 4123
Fragments arranged by size:
4123 2118 894 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2173 5158 5919 6947 1540
Size: 275 186 172 2985 761 1028 2301
Fragments arranged by size:
2985 2301 1028 761 275 186 172
BsiHKAI G_wGCw'C
Cuts at: 3627 5769 5854 6925 3627
Size: 2142 85 1071 4410
Fragments arranged by size:
4410 2142 1071 85
BsmI GAATG_Cn'
Cuts at: 3189 5219 5312 3189
Size: 2030 93 5585
Fragments arranged by size:
5585 2030 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2533 3627 3693 3966 3980 4271 4490
Size: 399 743 1094 66 273 14 291 219
Cuts at: 4490 4846 5027 5769 5854 6925 1391
Size: 356 181 742 85 1071 2174
Fragments arranged by size:
2174 1094 1071 743 742 399 356 291
273 219 181 85 66 14
BspEI T'CCGG_A
Cuts at: 7371 7371
Size: 7708
BspHI T'CATG_A
Cuts at: 2290 2824 3414 5597 7327 2290
Size: 534 590 2183 1730 2671
Fragments arranged by size:
2671 2183 1730 590 534
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 2509 3838 4448 5063 844
Size: 1322 343 1329 610 615 3489
Fragments arranged by size:
3489 1329 1322 615 610 343
BsrBI CCG'CTC
Cuts at: 1394 1823 2767 3199 4826 5595 1394
Size: 429 944 432 1627 769 3507
Fragments arranged by size:
3507 1627 944 769 432 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3226 3573 6203 6377 65
Size: 1183 1978 347 2630 174 1396
Fragments arranged by size:
2630 1978 1396 1183 347 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4500 6352 6539 1700
Size: 110 2690 1852 187 2869
Fragments arranged by size:
2869 2690 1852 187 110
BsrGI T'GTAC_A
Cuts at: 96 4143 96
Size: 4047 3661
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7708
BssSI C'ACGA_G
Cuts at: 5768 6780 5768
Size: 1012 6696
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2524 3832 4692 4962 794
Size: 1730 1308 860 270 3540
Fragments arranged by size:
3540 1730 1308 860 270
BstBI TT'CG_AA
Cuts at: 2311 6512 2311
Size: 4201 3507
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7708
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2837 3517 3908 4195 4292 4649 4973 2086
Size: 751 680 391 287 97 357 324 4821
Fragments arranged by size:
4821 751 680 391 357 324 287 97
BstYI r'GATC_y
Cuts at: 1972 2211 2920 2948 3471 3494 4242 4638 5038
Size: 239 709 28 523 23 748 396 400
Cuts at: 5038 5405 5790 5807 7259 7345 7357 7703 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 748 709 523 400 396 385
367 346 239 86 28 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 2639 2697 4235 2639
Size: 58 1538 6112
Fragments arranged by size:
6112 1538 58
BtgI C'CryG_G
Cuts at: 513 1065 2079 2253 2925 3406 3516 3726 4410
Size: 552 1014 174 672 481 110 210 684
Cuts at: 4410 513
Size: 3811
Fragments arranged by size:
3811 1014 684 672 552 481 210 174
110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2622 3367 487
Size: 683 56 1396 745 4828
Fragments arranged by size:
4828 1396 745 683 56
BtsI GCAGTG_nn'
Cuts at: 4117 4681 4796 5224 6018 6038 4117
Size: 564 115 428 794 20 5787
Fragments arranged by size:
5787 794 564 428 115 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2870 2905 529
Size: 35 2306 35 5332
Fragments arranged by size:
5332 2306 35 35
DraI TTT'AAA
Cuts at: 5012 5012
Size: 7708
DraIII CAC_nnn'GTG
Cuts at: 4141 4141
Size: 7708
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4983 6715 809
Size: 507 3667 1732 1802
Fragments arranged by size:
3667 1802 1732 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2139 2239
Size: 54 1425 50 275 18 291 18 100
Cuts at: 2239 4294 4450 5155 6044 7673 8
Size: 2055 156 705 889 1629 43
Fragments arranged by size:
2055 1629 1425 889 705 291 275 156
100 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 5155 1537
Size: 275 3343 4090
Fragments arranged by size:
4090 3343 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5638 1353
Size: 4285 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2197 4233 6295 6669 6815 387
Size: 1064 138 608 2036 2062 374 146 1280
Fragments arranged by size:
2062 2036 1280 1064 608 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2666 3481 3800 4112 4217 4812
Size: 393 618 416 815 319 312 105 595
Cuts at: 4812 4860 4898 5771 6368 7155 7423 1239
Size: 48 38 873 597 787 268 1524
Fragments arranged by size:
1524 873 815 787 618 597 595 416
393 319 312 268 105 48 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2659 2659
Size: 7708
EcoO109I rG'GnC_Cy
Cuts at: 3174 4267 4268 4374 4486 4487 4577 4902 3174
Size: 1093 1 106 112 1 90 325 5980
Fragments arranged by size:
5980 1093 325 112 106 90 1 1
EcoRI G'AATT_C
Cuts at: 2849 5017 5395 2849
Size: 2168 378 5162
Fragments arranged by size:
5162 2168 378
EcoRV GAT'ATC
Cuts at: 3943 3943
Size: 7708
FspI TGC'GCA
Cuts at: 6214 6214
Size: 7708
HaeII r_GCGC'y
Cuts at: 1678 4611 4756 6855 1678
Size: 2933 145 2099 2531
Fragments arranged by size:
2933 2531 2099 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2933 2965 3145
Size: 32 135 32 575 32 754 32 180
Cuts at: 3145 3177 3479 3511 4177 4209 4913 4945 6349
Size: 32 302 32 666 32 704 32 1404
Cuts at: 6349 6381 6423 6455 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1404 754 704 666 575 302 180
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5052 5298 669
Size: 988 281 3114 246 3079
Fragments arranged by size:
3114 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7708
HpaI GTT'AAC
Cuts at: 5298 5298
Size: 7708
KasI G'GCGC_C
Cuts at: 4607 4752 4607
Size: 145 7563
KpnI G_GTAC'C
Cuts at: 5033 5033
Size: 7708
MfeI C'AATT_G
Cuts at: 5307 5307
Size: 7708
MluI A'CGCG_T
Cuts at: 7584 7584
Size: 7708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 6822 7006 1305
Size: 3558 1959 184 2007
Fragments arranged by size:
3558 2007 1959 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2241 4452 10
Size: 54 1425 343 409 2211 3266
Fragments arranged by size:
3266 2211 1425 409 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2235 2258 2835 3462 4647 4767
Size: 648 918 151 23 577 627 1185 120
Cuts at: 4767 5666 6025 6184 518
Size: 899 359 159 2042
Fragments arranged by size:
2042 1185 918 899 648 627 577 359
159 151 120 23
MspA1I CmG'CkG
Cuts at: 1606 2733 4038 4067 5124 5801 6949 7194 1606
Size: 1127 1305 29 1057 677 1148 245 2120
Fragments arranged by size:
2120 1305 1148 1127 1057 677 245 29
NaeI GCC'GGC
Cuts at: 4502 4502
Size: 7708
NarI GG'CG_CC
Cuts at: 4608 4753 4608
Size: 145 7563
NcoI C'CATG_G
Cuts at: 513 1065 2079 2253 2925 3406 3726 513
Size: 552 1014 174 672 481 320 4495
Fragments arranged by size:
4495 1014 672 552 481 320 174
NdeI CA'TA_TG
Cuts at: 387 3451 387
Size: 3064 4644
NgoMIV G'CCGG_C
Cuts at: 4500 4500
Size: 7708
NheI G'CTAG_C
Cuts at: 1052 4280 1052
Size: 3228 4480
NotI GC'GGCC_GC
Cuts at: 5155 5155
Size: 7708
NspI r_CATG'y
Cuts at: 1132 1229 4770 5066 6611 1132
Size: 97 3541 296 1545 2229
Fragments arranged by size:
3541 2229 1545 296 97
PasI CC'CwG_GG
Cuts at: 1348 3686 4275 4603 4615 4634 1348
Size: 2338 589 328 12 19 4422
Fragments arranged by size:
4422 2338 589 328 19 12
PciI A'CATG_T
Cuts at: 4766 6607 4766
Size: 1841 5867
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2431 3221 3328 1108
Size: 1323 790 107 5488
Fragments arranged by size:
5488 1323 790 107
PfoI T'CCnGG_A
Cuts at: 4245 4564 4850 4245
Size: 319 286 7103
Fragments arranged by size:
7103 319 286
PmeI GTTT'AAAC
Cuts at: 5012 5012
Size: 7708
PmlI CAC'GTG
Cuts at: 2491 2491
Size: 7708
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5777 5809 7290 7322 5777
Size: 32 1481 32 6163
Fragments arranged by size:
6163 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4374 4374
Size: 7708
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7708
PsiI TTA'TAA
Cuts at: 5278 5278
Size: 7708
PspOMI G'GGCC_C
Cuts at: 4267 4486 4267
Size: 219 7489
PstI C_TGCA'G
Cuts at: 830 2520 4305 5060 830
Size: 1690 1785 755 3478
Fragments arranged by size:
3478 1785 1690 755
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7708
PvuII CAG'CTG
Cuts at: 2733 4038 4067 2733
Size: 1305 29 6374
Fragments arranged by size:
6374 1305 29
SalI G'TCGA_C
Cuts at: 1936 5050 1936
Size: 3114 4594
SbfI CC_TGCA'GG
Cuts at: 4305 5060 4305
Size: 755 6953
ScaI AGT'ACT
Cuts at: 2366 3905 5956 7668 2366
Size: 1539 2051 1712 2406
Fragments arranged by size:
2406 2051 1712 1539
SfcI C'TryA_G
Cuts at: 826 1045 2516 4301 5056 6191 6872 7063 826
Size: 219 1471 1785 755 1135 681 191 1471
Fragments arranged by size:
1785 1471 1471 1135 755 681 219 191
SfoI GGC'GCC
Cuts at: 4609 4754 4609
Size: 145 7563
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7708
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7708
SmaI CCC'GGG
Cuts at: 1872 4907 5035 1872
Size: 3035 128 4545
Fragments arranged by size:
4545 3035 128
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2976 2999 3072 4004 5812 6713
Size: 197 926 1033 23 73 932 1808 901
Cuts at: 6713 6975 820
Size: 262 1553
Fragments arranged by size:
1808 1553 1033 932 926 901 262 197
73 23
SnaBI TAC'GTA
Cuts at: 493 5499 493
Size: 5006 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7708
SphI G_CATG'C
Cuts at: 1132 5066 1132
Size: 3934 3774
SspI AAT'ATT
Cuts at: 5 52 5632 5
Size: 47 5580 2081
Fragments arranged by size:
5580 2081 47
StuI AGG'CCT
Cuts at: 1517 3893 1517
Size: 2376 5332
StyI C'CwwG_G
Cuts at: 513 1065 2079 2142 2253 2925 2982 3406 3726
Size: 552 1014 63 111 672 57 424 320
Cuts at: 3726 3894 4587 4645 5147 513
Size: 168 693 58 502 3074
Fragments arranged by size:
3074 1014 693 672 552 502 424 320
168 111 63 58 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 3562 4423 5735 5752 5905 6090
Size: 1255 1020 962 861 1312 17 153 185
Cuts at: 6090 6535 325
Size: 445 1498
Fragments arranged by size:
1498 1312 1255 1020 962 861 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2364 3903 4143
Size: 274 80 33 51 1443 387 1539 240
Cuts at: 4143 5954 7666 96
Size: 1811 1712 138
Fragments arranged by size:
1811 1712 1539 1443 387 274 240 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2434 2466 2557 2589 7668 7700 2434
Size: 32 91 32 5079 32 2442
Fragments arranged by size:
5079 2442 91 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3998 4372 1316
Size: 2682 374 4652
Fragments arranged by size:
4652 2682 374
XbaI T'CTAG_A
Cuts at: 3193 5044 3193
Size: 1851 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2932 3456 2932
Size: 524 7184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7708
XmaI C'CCGG_G
Cuts at: 1870 4905 5033 1870
Size: 3035 128 4545
Fragments arranged by size:
4545 3035 128
XmnI GAAnn'nnTTC
Cuts at: 5837 5837
Size: 7708
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5516 276
Size: 53 83 186 936 3982 2468
Fragments arranged by size:
3982 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
EcoICRI FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI