Restriction map for
pFN21AE9628
(Circular) (Six-base) MAPSORT of: pFN21AE9628.seq Check: 7360 from: 1 to: 7351
pFN21AE9628 7351 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5161 278
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2132 4232 4694 1937
Size: 195 2100 462 4594
Fragments arranged by size:
4594 2100 462 195
Acc65I G'GTAC_C
Cuts at: 2777 4672 2777
Size: 1895 5456
AclI AA'CG_TT
Cuts at: 1563 2442 5478 5851 1563
Size: 879 3036 373 3063
Fragments arranged by size:
3063 3036 879 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3054 5414 6798 7066 1632
Size: 1422 2360 1384 268 1917
Fragments arranged by size:
2360 1917 1422 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7154
AflIII A'CryG_T
Cuts at: 1203 4518 6250 7227 1203
Size: 3315 1732 977 1327
Fragments arranged by size:
3315 1732 1327 977
AgeI A'CCGG_T
Cuts at: 6182 6182
Size: 7351
AhdI GACnn_n'nnGTC
Cuts at: 6080 6080
Size: 7351
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2955 2987 2955
Size: 32 7319
AlwNI CAG_nnn'CTG
Cuts at: 5094 6666 5094
Size: 1572 5779
ApaLI G'TGCA_C
Cuts at: 5408 6564 5408
Size: 1156 6195
ApoI r'AATT_y
Cuts at: 1493 2510 3317 3380 4660 4886 5038 7147 1493
Size: 1017 807 63 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 1017 807 226 152 63
AseI AT'TA_AT
Cuts at: 160 1032 2957 5905 160
Size: 872 1925 2948 1606
Fragments arranged by size:
2948 1925 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 3406 4676 1870
Size: 73 88 1375 1270 4545
Fragments arranged by size:
4545 1375 1270 88 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2361 2394 3784 3817 2361
Size: 33 1390 33 5895
Fragments arranged by size:
5895 1390 33 33
BamHI G'GATC_C
Cuts at: 4681 5048 4681
Size: 367 6984
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2777 4672 6127 618
Size: 325 217 627 284 706 1895 1455 1842
Fragments arranged by size:
1895 1842 1455 706 627 325 284 217
BanII G_rGCy'C
Cuts at: 1391 2044 2118 3916 4481 4670 1391
Size: 653 74 1798 565 189 4072
Fragments arranged by size:
4072 1798 653 565 189 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3042 928
Size: 971 1143 5237
Fragments arranged by size:
5237 1143 971
BbvCI CC'TCA_GC
Cuts at: 3201 3201
Size: 7351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5542 5576 1903
Size: 34 50 34 3521 34 3678
Fragments arranged by size:
3678 3521 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 5243 6453 3758
Size: 1485 1210 4656
Fragments arranged by size:
4656 1485 1210
BclI T'GATC_A
Cuts at: 1548 2494 1548
Size: 946 6405
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5962 136
Size: 107 122 71 631 605 4290 1525
Fragments arranged by size:
4290 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4098 7346 4098
Size: 3248 4103
BlpI GC'TnA_GC
Cuts at: 4768 4768
Size: 7351
Bme1580I G_kGCm'C
Cuts at: 1790 5412 6568 1790
Size: 3622 1156 2573
Fragments arranged by size:
3622 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2655 1318
Size: 1337 6014
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 5082 6040
Size: 416 53 83 399 24 739 2926 958
Cuts at: 6040 442
Size: 1753
Fragments arranged by size:
2926 1753 958 739 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3932
Size: 7351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4115 4332 6011 1239
Size: 2876 217 1679 2579
Fragments arranged by size:
2876 2579 1679 217
Bpu10I CC'TnA_GC
Cuts at: 3201 3201
Size: 7351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2208 4495 5476 6341 6639 2208
Size: 2287 981 865 298 2920
Fragments arranged by size:
2920 2287 981 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6014 882
Size: 617 4515 2219
Fragments arranged by size:
4515 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5142 493
Size: 711 3938 2702
Fragments arranged by size:
3938 2702 711
BsaBI GATnn'nnATC
Cuts at: 2472 3746 7345 2472
Size: 1274 3599 2478
Fragments arranged by size:
3599 2478 1274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5158 5540 6175 275
Size: 53 83 186 936 3625 382 635 1451
Fragments arranged by size:
3625 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5784 6182 6456 6603 7014 5784
Size: 398 274 147 411 6121
Fragments arranged by size:
6121 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4237 4267 1178
Size: 30 165 30 2834 30 4262
Fragments arranged by size:
4262 2834 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2211 2214 2223 2226 3924 1186
Size: 1025 3 9 3 1698 4613
Fragments arranged by size:
4613 1698 1025 9 3 3
BseYI C'CCAG_C
Cuts at: 2252 3885 6554 7050 2252
Size: 1633 2669 496 2553
Fragments arranged by size:
2669 2553 1633 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6778
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2144 4801 5562 6590 1540
Size: 275 186 143 2657 761 1028 2301
Fragments arranged by size:
2657 2301 1028 761 275 186 143
BsiHKAI G_wGCw'C
Cuts at: 2092 2218 2594 5412 5497 6568 2092
Size: 126 376 2818 85 1071 2875
Fragments arranged by size:
2875 2818 1071 376 126 85
BsmI GAATG_Cn'
Cuts at: 4862 4955 4862
Size: 93 7258
BsmBI CGTCTCn'nnnn_
Cuts at: 4643 4643
Size: 7351
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2092 2118 2218 2594 3916 4481
Size: 399 254 48 26 100 376 1322 565
Cuts at: 4481 4670 5412 5497 6568 1391
Size: 189 742 85 1071 2174
Fragments arranged by size:
2174 1322 1071 742 565 399 376 254
189 100 85 48 26
BspEI T'CCGG_A
Cuts at: 7014 7014
Size: 7351
BspHI T'CATG_A
Cuts at: 5240 6970 5240
Size: 1730 5621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4706 844
Size: 3862 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2572 5238 1394
Size: 429 749 2666 3507
Fragments arranged by size:
3507 2666 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3522 4202 5846 6020 65
Size: 1183 2274 680 1644 174 1396
Fragments arranged by size:
2274 1644 1396 1183 680 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5995 6182 1700
Size: 110 4185 187 2869
Fragments arranged by size:
4185 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7351
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7351
BssSI C'ACGA_G
Cuts at: 4589 5411 6423 4589
Size: 822 1012 5517
Fragments arranged by size:
5517 1012 822
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7351
BstBI TT'CG_AA
Cuts at: 6155 6155
Size: 7351
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7351
BstYI r'GATC_y
Cuts at: 1972 4098 4681 5048 5433 5450 6902 6988 7000
Size: 2126 583 367 385 17 1452 86 12
Cuts at: 7000 7346 1972
Size: 346 1977
Fragments arranged by size:
2126 1977 1452 583 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2247 2247
Size: 7351
BtgI C'CryG_G
Cuts at: 513 1065 3609 513
Size: 552 2544 4255
Fragments arranged by size:
4255 2544 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6612
Fragments arranged by size:
6612 683 56
BtsI GCAGTG_nn'
Cuts at: 3701 4867 5661 5681 3701
Size: 1166 794 20 5371
Fragments arranged by size:
5371 1166 794 20
ClaI AT'CG_AT
Cuts at: 1554 4145 1554
Size: 2591 4760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7316
DraI TTT'AAA
Cuts at: 4655 4655
Size: 7351
DraIII CAC_nnn'GTG
Cuts at: 3179 3179
Size: 7351
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6358 809
Size: 507 5042 1802
Fragments arranged by size:
5042 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2141 2558 4798
Size: 54 1425 50 275 18 311 417 2240
Cuts at: 4798 5687 7316 8
Size: 889 1629 43
Fragments arranged by size:
2240 1629 1425 889 417 311 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2141 4798 1537
Size: 275 329 2657 4090
Fragments arranged by size:
4090 2657 329 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3406 4071 5281 1353
Size: 2053 665 1210 3423
Fragments arranged by size:
3423 2053 1210 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5938 6312 6458 387
Size: 1064 138 4349 374 146 1280
Fragments arranged by size:
4349 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3054 4115 4332 5414 6011 6798 7066
Size: 393 1422 1061 217 1082 597 787 268
Cuts at: 7066 1239
Size: 1524
Fragments arranged by size:
1524 1422 1082 1061 787 597 393 268
217
EcoNI CCTnn'n_nnAGG
Cuts at: 3920 3920
Size: 7351
EcoO109I rG'GnC_Cy
Cuts at: 2081 3160 2081
Size: 1079 6272
EcoRI G'AATT_C
Cuts at: 3317 4660 5038 3317
Size: 1343 378 5630
Fragments arranged by size:
5630 1343 378
EcoRV GAT'ATC
Cuts at: 2409 3979 2409
Size: 1570 5781
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4223 4223
Size: 7351
FspI TGC'GCA
Cuts at: 5857 5857
Size: 7351
HaeII r_GCGC'y
Cuts at: 1678 2270 2524 4559 6498 1678
Size: 592 254 2035 1939 2531
Fragments arranged by size:
2531 2035 1939 592 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 2525 2557 3647
Size: 32 135 32 826 32 95 32 1090
Cuts at: 3647 3679 5992 6024 6066 6098 1373
Size: 32 2313 32 42 32 2626
Fragments arranged by size:
2626 2313 1090 826 135 95 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2133 2446 4233 4374 4632 4695
Size: 988 281 195 313 1787 141 258 63
Cuts at: 4695 4941 669
Size: 246 3079
Fragments arranged by size:
3079 1787 988 313 281 258 246 195
141 63
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7351
HpaI GTT'AAC
Cuts at: 4941 4941
Size: 7351
KpnI G_GTAC'C
Cuts at: 2781 4676 2781
Size: 1895 5456
MfeI C'AATT_G
Cuts at: 2967 4950 2967
Size: 1983 5368
MluI A'CGCG_T
Cuts at: 7227 7227
Size: 7351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6465 6649 1305
Size: 5160 184 2007
Fragments arranged by size:
5160 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5529
Fragments arranged by size:
5529 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3620 4283 5309 5668 5827 518
Size: 648 2454 663 1026 359 159 2042
Fragments arranged by size:
2454 2042 1026 663 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 3205 4767 5444 6592 6837 1606
Size: 540 1059 1562 677 1148 245 2120
Fragments arranged by size:
2120 1562 1148 1059 677 540 245
NcoI C'CATG_G
Cuts at: 513 1065 3609 513
Size: 552 2544 4255
Fragments arranged by size:
4255 2544 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7351
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7351
NotI GC'GGCC_GC
Cuts at: 4798 4798
Size: 7351
NspI r_CATG'y
Cuts at: 1132 1229 2342 4522 4709 6254 1132
Size: 97 1113 2180 187 1545 2229
Fragments arranged by size:
2229 2180 1545 1113 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7351
PciI A'CATG_T
Cuts at: 4518 6250 4518
Size: 1732 5619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3829 4579 1108
Size: 2721 750 3880
Fragments arranged by size:
3880 2721 750
PfoI T'CCnGG_A
Cuts at: 4348 4348
Size: 7351
PmeI GTTT'AAAC
Cuts at: 4655 4655
Size: 7351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5420 5452 6933 6965 5420
Size: 32 1481 32 5806
Fragments arranged by size:
5806 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2081
Size: 7351
PshAI GACnn'nnGTC
Cuts at: 1613 3869 1613
Size: 2256 5095
PsiI TTA'TAA
Cuts at: 4449 4527 4921 4449
Size: 78 394 6879
Fragments arranged by size:
6879 394 78
PstI C_TGCA'G
Cuts at: 830 2151 2181 3708 4703 830
Size: 1321 30 1527 995 3478
Fragments arranged by size:
3478 1527 1321 995 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7351
PvuII CAG'CTG
Cuts at: 3205 3205
Size: 7351
SalI G'TCGA_C
Cuts at: 1936 2131 4231 4693 1936
Size: 195 2100 462 4594
Fragments arranged by size:
4594 2100 462 195
SanDI GG'GwC_CC
Cuts at: 2081 2081
Size: 7351
SapI GCTCTTCn'nnn_
Cuts at: 4071 4071
Size: 7351
SbfI CC_TGCA'GG
Cuts at: 4703 4703
Size: 7351
ScaI AGT'ACT
Cuts at: 5599 7311 5599
Size: 1712 5639
SexAI A'CCwGG_T
Cuts at: 2307 2307
Size: 7351
SfcI C'TryA_G
Cuts at: 826 1045 2147 2177 2998 3085 3544 3590 3704
Size: 219 1102 30 821 87 459 46 114
Cuts at: 3704 4699 5834 6515 6706 826
Size: 995 1135 681 191 1471
Fragments arranged by size:
1471 1135 1102 995 821 681 459 219
191 114 87 46 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7351
SmaI CCC'GGG
Cuts at: 1872 3408 4678 1872
Size: 1536 1270 4545
Fragments arranged by size:
4545 1536 1270
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2223 4474 5455 6356 6618 820
Size: 197 926 280 2251 981 901 262 1553
Fragments arranged by size:
2251 1553 981 926 901 280 262 197
SnaBI TAC'GTA
Cuts at: 493 5142 493
Size: 4649 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7351
SphI G_CATG'C
Cuts at: 1132 4709 1132
Size: 3577 3774
SspI AAT'ATT
Cuts at: 5 52 5275 5
Size: 47 5223 2081
Fragments arranged by size:
5223 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7351
StyI C'CwwG_G
Cuts at: 513 1065 3609 4790 513
Size: 552 2544 1181 3074
Fragments arranged by size:
3074 2544 1181 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5378 5395 5548 5733 6178 325
Size: 1255 3798 17 153 185 445 1498
Fragments arranged by size:
3798 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4489 5597 7309
Size: 274 80 33 51 1443 2512 1108 1712
Cuts at: 7309 96
Size: 138
Fragments arranged by size:
2512 1712 1443 1108 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4112 4144 7311 7343 4112
Size: 32 3167 32 4120
Fragments arranged by size:
4120 3167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7351
XbaI T'CTAG_A
Cuts at: 4687 4687
Size: 7351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2531 3528 2531
Size: 997 6354
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7351
XmaI C'CCGG_G
Cuts at: 1870 3406 4676 1870
Size: 1536 1270 4545
Fragments arranged by size:
4545 1536 1270
XmnI GAAnn'nnTTC
Cuts at: 5480 5480
Size: 7351
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5159 276
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BsiWI
BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SfiI SfoI SrfI SwaI