Restriction map for
pFN21AE9626
(Circular) (Six-base) MAPSORT of: pFN21AE9626.seq Check: 459 from: 1 to: 7285
pFN21AE9626 7285 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3820 3820
Size: 7285
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5095 278
Size: 53 83 186 936 3559 2468
Fragments arranged by size:
3559 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2142 2251 2954 4628 1937
Size: 205 109 703 1674 4594
Fragments arranged by size:
4594 1674 703 205 109
Acc65I G'GTAC_C
Cuts at: 4280 4606 4280
Size: 326 6959
AclI AA'CG_TT
Cuts at: 1563 5412 5785 1563
Size: 3849 373 3063
Fragments arranged by size:
3849 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2945 2987 4328 4503 5348 6732 7000 1632
Size: 1313 42 1341 175 845 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1313 845 268 175 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7088
AflIII A'CryG_T
Cuts at: 1203 3238 3607 6184 7161 1203
Size: 2035 369 2577 977 1327
Fragments arranged by size:
2577 2035 1327 977 369
AgeI A'CCGG_T
Cuts at: 6116 6116
Size: 7285
AhdI GACnn_n'nnGTC
Cuts at: 6014 6014
Size: 7285
AleI CACnn'nnGTG
Cuts at: 1166 2534 1166
Size: 1368 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7285
AlwNI CAG_nnn'CTG
Cuts at: 3882 5028 6600 3882
Size: 1146 1572 4567
Fragments arranged by size:
4567 1572 1146
ApaI G_GGCC'C
Cuts at: 2038 2038
Size: 7285
ApaLI G'TGCA_C
Cuts at: 5342 6498 5342
Size: 1156 6129
ApoI r'AATT_y
Cuts at: 1493 2381 2777 4594 4820 4972 7081 1493
Size: 888 396 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 888 396 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5839 160
Size: 872 4807 1606
Fragments arranged by size:
4807 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
AvaI C'yCGr_G
Cuts at: 1870 1943 2391 4610 1870
Size: 73 448 2219 4545
Fragments arranged by size:
4545 2219 448 73
AvrII C'CTAG_G
Cuts at: 2180 2180
Size: 7285
BamHI G'GATC_C
Cuts at: 4615 4982 4615
Size: 367 6918
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2075 2403 4280 4606
Size: 325 217 627 266 22 328 1877 326
Cuts at: 4606 6061 618
Size: 1455 1842
Fragments arranged by size:
1877 1842 1455 627 328 326 325 266
217 22
BanII G_rGCy'C
Cuts at: 1391 2038 3516 4604 1391
Size: 647 1478 1088 4072
Fragments arranged by size:
4072 1478 1088 647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2068 3587 928
Size: 971 169 1519 4626
Fragments arranged by size:
4626 1519 971 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2648 2682 4059 4093 5476 5510 1903
Size: 34 711 34 1377 34 1383 34 3678
Fragments arranged by size:
3678 1383 1377 711 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5177 6387 5177
Size: 1210 6075
BclI T'GATC_A
Cuts at: 1548 3099 1548
Size: 1551 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5896 136
Size: 107 122 71 631 605 4224 1525
Fragments arranged by size:
4224 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3116 7280 3116
Size: 4164 3121
BlpI GC'TnA_GC
Cuts at: 4702 4702
Size: 7285
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2058 5346 6502 1790
Size: 248 20 3288 1156 2573
Fragments arranged by size:
3288 2573 1156 248 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5016 5974 442
Size: 416 53 83 399 24 3599 958 1753
Fragments arranged by size:
3599 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 3245 5945 1239
Size: 1305 701 2700 2579
Fragments arranged by size:
2700 2579 1305 701
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 7285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3515 5410 6275 6573 3515
Size: 1895 865 298 4227
Fragments arranged by size:
4227 1895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2238 3898 5948 882
Size: 617 739 1660 2050 2219
Fragments arranged by size:
2219 2050 1660 739 617
BsaAI yAC'GTr
Cuts at: 493 1204 4378 5076 493
Size: 711 3174 698 2702
Fragments arranged by size:
3174 2702 711 698
BsaBI GATnn'nnATC
Cuts at: 2766 3458 7279 2766
Size: 692 3821 2772
Fragments arranged by size:
3821 2772 692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5092 5474 6109 275
Size: 53 83 186 936 3559 382 635 1451
Fragments arranged by size:
3559 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5718 6116 6390 6537 6948 5718
Size: 398 274 147 411 6055
Fragments arranged by size:
6055 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3243 3273 3783 3813 3920
Size: 30 165 30 1840 30 510 30 107
Cuts at: 3920 3950 1178
Size: 30 4513
Fragments arranged by size:
4513 1840 510 165 107 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3666 1186
Size: 2480 4805
BseYI C'CCAG_C
Cuts at: 2030 2037 4198 6488 6984 2030
Size: 7 2161 2290 496 2331
Fragments arranged by size:
2331 2290 2161 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3105 3412 4392 1163
Size: 573 1369 307 980 4056
Fragments arranged by size:
4056 1369 980 573 307
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4735 5496 6524 1540
Size: 275 186 2734 761 1028 2301
Fragments arranged by size:
2734 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5346 5431 6502 5346
Size: 85 1071 6129
Fragments arranged by size:
6129 1071 85
BsiWI C'GTAC_G
Cuts at: 4378 4378
Size: 7285
BsmI GAATG_Cn'
Cuts at: 4796 4889 4796
Size: 93 7192
BsmBI CGTCTCn'nnnn_
Cuts at: 2827 2827
Size: 7285
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2058 3516 4604 5346 5431 6502
Size: 399 248 20 1458 1088 742 85 1071
Cuts at: 6502 1391
Size: 2174
Fragments arranged by size:
2174 1458 1088 1071 742 399 248 85
20
BspEI T'CCGG_A
Cuts at: 6948 6948
Size: 7285
BspHI T'CATG_A
Cuts at: 5174 6904 5174
Size: 1730 5555
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3030 3820 4030 4414 4640 844
Size: 2186 790 210 384 226 3489
Fragments arranged by size:
3489 2186 790 384 226 210
BsrBI CCG'CTC
Cuts at: 1394 1823 2558 5172 1394
Size: 429 735 2614 3507
Fragments arranged by size:
3507 2614 735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4516 5780 5954 65
Size: 1183 3268 1264 174 1396
Fragments arranged by size:
3268 1396 1264 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5929 6116 1700
Size: 110 4119 187 2869
Fragments arranged by size:
4119 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4385 4543 96
Size: 4289 158 2838
Fragments arranged by size:
4289 2838 158
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7285
BssSI C'ACGA_G
Cuts at: 5345 6357 5345
Size: 1012 6273
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2410 3119 794
Size: 1616 709 4960
Fragments arranged by size:
4960 1616 709
BstBI TT'CG_AA
Cuts at: 6089 6089
Size: 7285
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7285
BstYI r'GATC_y
Cuts at: 1972 3116 3178 4519 4615 4982 5367 5384 6836
Size: 1144 62 1341 96 367 385 17 1452
Cuts at: 6836 6922 6934 7280 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1341 1144 385 367 346 96
86 62 17 12
BstZ17I GTA'TAC
Cuts at: 2955 2955
Size: 7285
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 3301 3939 513
Size: 552 939 75 1222 638 3859
Fragments arranged by size:
3859 1222 939 638 552 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6546
Fragments arranged by size:
6546 683 56
BtsI GCAGTG_nn'
Cuts at: 2670 3395 4801 5595 5615 2670
Size: 725 1406 794 20 4340
Fragments arranged by size:
4340 1406 794 725 20
ClaI AT'CG_AT
Cuts at: 1554 2658 1554
Size: 1104 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7250
DraI TTT'AAA
Cuts at: 2227 3205 4589 2227
Size: 978 1384 4923
Fragments arranged by size:
4923 1384 978
DraIII CAC_nnn'GTG
Cuts at: 3644 3644
Size: 7285
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6292 809
Size: 507 4976 1802
Fragments arranged by size:
4976 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2908 4732 5621
Size: 54 1425 50 275 18 1078 1824 889
Cuts at: 5621 7250 8
Size: 1629 43
Fragments arranged by size:
1824 1629 1425 1078 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4732 1537
Size: 275 2920 4090
Fragments arranged by size:
4090 2920 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2964 4127 5215 1353
Size: 1611 1163 1088 3423
Fragments arranged by size:
3423 1611 1163 1088
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5872 6246 6392 387
Size: 1064 138 4283 374 146 1280
Fragments arranged by size:
4283 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2544 2945 2987 3245 4328 4503 5348
Size: 393 912 401 42 258 1083 175 845
Cuts at: 5348 5945 6732 7000 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1083 912 845 787 597 401 393
268 258 175 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 2343 2583 2150
Size: 193 240 6852
Fragments arranged by size:
6852 240 193
EcoO109I rG'GnC_Cy
Cuts at: 2024 2034 2024
Size: 10 7275
EcoRI G'AATT_C
Cuts at: 4594 4972 4594
Size: 378 6907
EcoRV GAT'ATC
Cuts at: 4451 4451
Size: 7285
FspI TGC'GCA
Cuts at: 5791 5791
Size: 7285
HaeII r_GCGC'y
Cuts at: 1678 6432 1678
Size: 4754 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2749 2781 3333 3365 3783
Size: 32 135 32 1177 32 552 32 418
Cuts at: 3783 3815 3903 3920 3935 3952 5926 5958 6000
Size: 32 88 17 15 17 1974 32 42
Cuts at: 6000 6032 1373
Size: 32 2626
Fragments arranged by size:
2626 1974 1177 552 418 135 88 42
32 32 32 32 32 32 32 17
17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 3975 4629 4875 669
Size: 988 281 268 1769 654 246 3079
Fragments arranged by size:
3079 1769 988 654 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7285
HpaI GTT'AAC
Cuts at: 4875 4875
Size: 7285
KpnI G_GTAC'C
Cuts at: 4284 4610 4284
Size: 326 6959
MfeI C'AATT_G
Cuts at: 4884 4884
Size: 7285
MluI A'CGCG_T
Cuts at: 3238 7161 3238
Size: 3923 3362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4159 6399 6583 1305
Size: 2854 2240 184 2007
Fragments arranged by size:
2854 2240 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5463
Fragments arranged by size:
5463 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 4236 4302 4476 5243 5602 5761
Size: 648 1368 1702 66 174 767 359 159
Cuts at: 5761 518
Size: 2042
Fragments arranged by size:
2042 1702 1368 767 648 359 174 159
66
MspA1I CmG'CkG
Cuts at: 1606 2030 2965 3427 4202 4348 4701 5378 6526
Size: 424 935 462 775 146 353 677 1148
Cuts at: 6526 6771 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 935 775 677 462 424 353
245 146
NcoI C'CATG_G
Cuts at: 513 1065 2004 2079 3301 3939 513
Size: 552 939 75 1222 638 3859
Fragments arranged by size:
3859 1222 939 638 552 75
NdeI CA'TA_TG
Cuts at: 387 4237 387
Size: 3850 3435
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7285
NotI GC'GGCC_GC
Cuts at: 4732 4732
Size: 7285
NspI r_CATG'y
Cuts at: 1132 1229 3319 4643 6188 1132
Size: 97 2090 1324 1545 2229
Fragments arranged by size:
2229 2090 1545 1324 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7285
PciI A'CATG_T
Cuts at: 6184 6184
Size: 7285
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2581 2917 3815 3931 1108
Size: 1473 336 898 116 4462
Fragments arranged by size:
4462 1473 898 336 116
PfoI T'CCnGG_A
Cuts at: 3924 3945 3924
Size: 21 7264
PmeI GTTT'AAAC
Cuts at: 4589 4589
Size: 7285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5354 5386 6867 6899 3503
Size: 32 1819 32 1481 32 3889
Fragments arranged by size:
3889 1819 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2024
Size: 7285
PshAI GACnn'nnGTC
Cuts at: 1613 2175 1613
Size: 562 6723
PsiI TTA'TAA
Cuts at: 3969 4855 3969
Size: 886 6399
PspOMI G'GGCC_C
Cuts at: 2034 2034
Size: 7285
PstI C_TGCA'G
Cuts at: 830 4336 4637 830
Size: 3506 301 3478
Fragments arranged by size:
3506 3478 301
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7285
PvuII CAG'CTG
Cuts at: 2965 3427 4202 4348 2965
Size: 462 775 146 5902
Fragments arranged by size:
5902 775 462 146
SalI G'TCGA_C
Cuts at: 1936 4627 1936
Size: 2691 4594
SbfI CC_TGCA'GG
Cuts at: 4637 4637
Size: 7285
ScaI AGT'ACT
Cuts at: 2421 5533 7245 2421
Size: 3112 1712 2461
Fragments arranged by size:
3112 2461 1712
SexAI A'CCwGG_T
Cuts at: 4428 4428
Size: 7285
SfcI C'TryA_G
Cuts at: 826 1045 2310 3551 4258 4332 4633 5768 6449
Size: 219 1265 1241 707 74 301 1135 681
Cuts at: 6449 6640 826
Size: 191 1471
Fragments arranged by size:
1471 1265 1241 1135 707 681 301 219
191 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7285
SmaI CCC'GGG
Cuts at: 1872 4612 1872
Size: 2740 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3530 5389 6290 6552 820
Size: 197 926 1587 1859 901 262 1553
Fragments arranged by size:
1859 1587 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4378 5076 493
Size: 3885 698 2702
Fragments arranged by size:
3885 2702 698
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7285
SphI G_CATG'C
Cuts at: 1132 4643 1132
Size: 3511 3774
SspI AAT'ATT
Cuts at: 5 52 2734 3043 3405 3736 5209 5
Size: 47 2682 309 362 331 1473 2081
Fragments arranged by size:
2682 2081 1473 362 331 309 47
StuI AGG'CCT
Cuts at: 1517 2869 1517
Size: 1352 5933
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2180 3301 3939 4536 4724
Size: 552 939 75 101 1121 638 597 188
Cuts at: 4724 513
Size: 3074
Fragments arranged by size:
3074 1121 939 638 597 552 188 101
75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3885 4248 5312 5329 5482 5667 6112
Size: 1255 2305 363 1064 17 153 185 445
Cuts at: 6112 325
Size: 1498
Fragments arranged by size:
2305 1498 1255 1064 445 363 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2419 4385 4543
Size: 274 80 33 51 1443 442 1966 158
Cuts at: 4543 4575 5531 7243 96
Size: 32 956 1712 138
Fragments arranged by size:
1966 1712 1443 956 442 274 158 138
80 51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2275 2307 2830 2862 3243 3275 7245 7277 2275
Size: 32 523 32 381 32 3970 32 2283
Fragments arranged by size:
3970 2283 523 381 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7285
XbaI T'CTAG_A
Cuts at: 4621 4621
Size: 7285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 2098
Size: 7285
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7285
XmaI C'CCGG_G
Cuts at: 1870 4610 1870
Size: 2740 4545
XmnI GAAnn'nnTTC
Cuts at: 3329 3413 5414 3329
Size: 84 2001 5200
Fragments arranged by size:
5200 2001 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5093 276
Size: 53 83 186 936 3559 2468
Fragments arranged by size:
3559 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BstXI Bsu36I EcoICRI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI