Restriction map for
pFN21AE9625
(Circular) (Six-base) MAPSORT of: pFN21AE9625.seq Check: 3798 from: 1 to: 7042
pFN21AE9625 7042 bp
With 182 enzymes: SgfI *
March 28, 2012 12:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4852 278
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4385 1937
Size: 2448 4594
Acc65I G'GTAC_C
Cuts at: 4363 4363
Size: 7042
AclI AA'CG_TT
Cuts at: 1563 5169 5542 1563
Size: 3606 373 3063
Fragments arranged by size:
3606 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2226 2771 2895 3129 3730 4053 4128 5105
Size: 594 545 124 234 601 323 75 977
Cuts at: 5105 6489 6757 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 977 601 594 545 323 268
234 124 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6845
AflIII A'CryG_T
Cuts at: 1203 5941 6918 1203
Size: 4738 977 1327
Fragments arranged by size:
4738 1327 977
AgeI A'CCGG_T
Cuts at: 2270 5873 2270
Size: 3603 3439
AhdI GACnn_n'nnGTC
Cuts at: 5771 5771
Size: 7042
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7042
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2449 1184
Size: 1265 5777
AlwNI CAG_nnn'CTG
Cuts at: 2179 2416 2554 2746 3892 4785 6357 2179
Size: 237 138 192 1146 893 1572 2864
Fragments arranged by size:
2864 1572 1146 893 237 192 138
ApaLI G'TGCA_C
Cuts at: 5099 6255 5099
Size: 1156 5886
ApoI r'AATT_y
Cuts at: 1493 2147 2276 3113 3258 3382 4351 4577 4729
Size: 654 129 837 145 124 969 226 152
Cuts at: 4729 6838 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 969 837 654 226 152 145
129 124
AseI AT'TA_AT
Cuts at: 160 1032 3461 5596 160
Size: 872 2429 2135 1606
Fragments arranged by size:
2429 2135 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
AvaI C'yCGr_G
Cuts at: 1870 1943 3668 4367 1870
Size: 73 1725 699 4545
Fragments arranged by size:
4545 1725 699 73
BamHI G'GATC_C
Cuts at: 4372 4739 4372
Size: 367 6675
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4363 5818 618
Size: 325 217 627 2576 1455 1842
Fragments arranged by size:
2576 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2502 4361 1391
Size: 1111 1859 4072
Fragments arranged by size:
4072 1859 1111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 2663 928
Size: 971 315 449 5307
Fragments arranged by size:
5307 971 449 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5233 5267 1903
Size: 34 3296 34 3678
Fragments arranged by size:
3678 3296 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3364 4934 6144 3364
Size: 1570 1210 4262
Fragments arranged by size:
4262 1570 1210
BclI T'GATC_A
Cuts at: 1548 3508 1548
Size: 1960 5082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5653 136
Size: 107 122 71 631 605 3981 1525
Fragments arranged by size:
3981 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7037 7037
Size: 7042
BlpI GC'TnA_GC
Cuts at: 2409 4459 2409
Size: 2050 4992
Bme1580I G_kGCm'C
Cuts at: 1790 3790 5103 6259 1790
Size: 2000 1313 1156 2573
Fragments arranged by size:
2573 2000 1313 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7042
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3604 4773 5731
Size: 416 53 83 399 24 2187 1169 958
Cuts at: 5731 442
Size: 1753
Fragments arranged by size:
2187 1753 1169 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2897 5702 1239
Size: 1658 2805 2579
Fragments arranged by size:
2805 2579 1658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5167 6032 6330 5167
Size: 865 298 5879
Fragments arranged by size:
5879 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5705 882
Size: 617 4206 2219
Fragments arranged by size:
4206 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2263 4833 493
Size: 711 1059 2570 2702
Fragments arranged by size:
2702 2570 1059 711
BsaBI GATnn'nnATC
Cuts at: 7036 7036
Size: 7042
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4849 5231 5866 275
Size: 53 83 186 936 3316 382 635 1451
Fragments arranged by size:
3316 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2270 5475 5873 6147 6294 6705 2270
Size: 3205 398 274 147 411 2607
Fragments arranged by size:
3205 2607 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3002 3032 1178
Size: 30 165 30 1599 30 5188
Fragments arranged by size:
5188 1599 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2666 1186
Size: 928 552 5562
Fragments arranged by size:
5562 928 552
BseYI C'CCAG_C
Cuts at: 6245 6741 6245
Size: 496 6546
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3878 1163
Size: 573 2142 4327
Fragments arranged by size:
4327 2142 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4492 5253 6281 1540
Size: 275 186 2491 761 1028 2301
Fragments arranged by size:
2491 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2410 2502 2913 5103 5188 6259 2410
Size: 92 411 2190 85 1071 3193
Fragments arranged by size:
3193 2190 1071 411 92 85
BsmI GAATG_Cn'
Cuts at: 3770 4553 4646 3770
Size: 783 93 6166
Fragments arranged by size:
6166 783 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2410 2502 2913 3790 4361 5103 5188
Size: 399 620 92 411 877 571 742 85
Cuts at: 5188 6259 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 877 742 620 571 411 399
92 85
BspEI T'CCGG_A
Cuts at: 6705 6705
Size: 7042
BspHI T'CATG_A
Cuts at: 4931 6661 4931
Size: 1730 5312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2226 3877 4397 844
Size: 1382 1651 520 3489
Fragments arranged by size:
3489 1651 1382 520
BsrBI CCG'CTC
Cuts at: 1394 1823 4929 1394
Size: 429 3106 3507
Fragments arranged by size:
3507 3106 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3645 5537 5711 65
Size: 1183 2397 1892 174 1396
Fragments arranged by size:
2397 1892 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2270 5686 5873 1700
Size: 110 460 3416 187 2869
Fragments arranged by size:
3416 2869 460 187 110
BsrGI T'GTAC_A
Cuts at: 96 3642 96
Size: 3546 3496
BssHII G'CGCG_C
Cuts at: 1925 2169 1925
Size: 244 6798
BssSI C'ACGA_G
Cuts at: 5102 6114 5102
Size: 1012 6030
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3892 794
Size: 3098 3944
BstBI TT'CG_AA
Cuts at: 5846 5846
Size: 7042
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7042
BstXI CCAn_nnnn'nTGG
Cuts at: 2596 2596
Size: 7042
BstYI r'GATC_y
Cuts at: 1972 2059 2254 3730 4372 4739 5124 5141 6593
Size: 87 195 1476 642 367 385 17 1452
Cuts at: 6593 6679 6691 7037 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1476 1452 642 385 367 346 195
87 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2762 2762
Size: 7042
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5551
Fragments arranged by size:
5551 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6303
Fragments arranged by size:
6303 683 56
BtsI GCAGTG_nn'
Cuts at: 2352 3714 4558 5352 5372 2352
Size: 1362 844 794 20 4022
Fragments arranged by size:
4022 1362 844 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7042
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2341 2376 529
Size: 35 1777 35 5195
Fragments arranged by size:
5195 1777 35 35
DraI TTT'AAA
Cuts at: 2472 3147 4346 2472
Size: 675 1199 5168
Fragments arranged by size:
5168 1199 675
DraIII CAC_nnn'GTG
Cuts at: 3376 3376
Size: 7042
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2315 6049 809
Size: 507 999 3734 1802
Fragments arranged by size:
3734 1802 999 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4489 5378 7007
Size: 54 1425 50 275 18 2659 889 1629
Cuts at: 7007 8
Size: 43
Fragments arranged by size:
2659 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4489 1537
Size: 275 2677 4090
Fragments arranged by size:
4090 2677 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 2535 3084 3846 4131 4267 4309 4972
Size: 1062 120 549 762 285 136 42 663
Cuts at: 4972 1353
Size: 3423
Fragments arranged by size:
3423 1062 762 663 549 285 136 120
42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2237 5629 6003 6149 387
Size: 1064 138 648 3392 374 146 1280
Fragments arranged by size:
3392 1280 1064 648 374 146 138
EcoICRI GAG'CTC
Cuts at: 2500 2500
Size: 7042
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2226 2771 2895 2897 3129 3730 4053
Size: 393 594 545 124 2 232 601 323
Cuts at: 4053 4128 5105 5702 6489 6757 1239
Size: 75 977 597 787 268 1524
Fragments arranged by size:
1524 977 787 601 597 594 545 393
323 268 232 124 75 2
EcoO109I rG'GnC_Cy
Cuts at: 2067 2176 4082 4241 2067
Size: 109 1906 159 4868
Fragments arranged by size:
4868 1906 159 109
EcoRI G'AATT_C
Cuts at: 2147 4351 4729 2147
Size: 2204 378 4460
Fragments arranged by size:
4460 2204 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3818 3818
Size: 7042
FspI TGC'GCA
Cuts at: 5548 5548
Size: 7042
HaeII r_GCGC'y
Cuts at: 1678 6189 1678
Size: 4511 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2988 3002 3020
Size: 32 135 32 614 32 770 14 18
Cuts at: 3020 3034 4010 4042 5683 5715 5757 5789 1373
Size: 14 976 32 1641 32 42 32 2626
Fragments arranged by size:
2626 1641 976 770 614 135 42 32
32 32 32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4386 4632 669
Size: 988 281 2448 246 3079
Fragments arranged by size:
3079 2448 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2723 4278 748
Size: 1975 1555 3512
Fragments arranged by size:
3512 1975 1555
HpaI GTT'AAC
Cuts at: 4632 4632
Size: 7042
KpnI G_GTAC'C
Cuts at: 4367 4367
Size: 7042
MfeI C'AATT_G
Cuts at: 4641 4641
Size: 7042
MluI A'CGCG_T
Cuts at: 6918 6918
Size: 7042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 3083 6156 6340 1305
Size: 975 803 3073 184 2007
Fragments arranged by size:
3073 2007 975 803 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5220
Fragments arranged by size:
5220 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2431 5000 5359 5518 518
Size: 648 1265 2569 359 159 2042
Fragments arranged by size:
2569 2042 1265 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2770 4253 4322 4458 5135 6283 6528 1606
Size: 1164 1483 69 136 677 1148 245 2120
Fragments arranged by size:
2120 1483 1164 1148 677 245 136 69
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5551
Fragments arranged by size:
5551 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7042
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7042
NotI GC'GGCC_GC
Cuts at: 4489 4489
Size: 7042
NspI r_CATG'y
Cuts at: 1132 1229 2164 3435 4400 5945 1132
Size: 97 935 1271 965 1545 2229
Fragments arranged by size:
2229 1545 1271 965 935 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7042
PciI A'CATG_T
Cuts at: 5941 5941
Size: 7042
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7042
PfoI T'CCnGG_A
Cuts at: 2039 2228 2039
Size: 189 6853
PmeI GTTT'AAAC
Cuts at: 4346 4346
Size: 7042
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5111 5143 6624 6656 5111
Size: 32 1481 32 5497
Fragments arranged by size:
5497 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2067 4082 4241 2067
Size: 2015 159 4868
Fragments arranged by size:
4868 2015 159
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7042
PsiI TTA'TAA
Cuts at: 4612 4612
Size: 7042
PstI C_TGCA'G
Cuts at: 830 3868 4394 830
Size: 3038 526 3478
Fragments arranged by size:
3478 3038 526
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7042
PvuII CAG'CTG
Cuts at: 4253 4322 4253
Size: 69 6973
SacI G_AGCT'C
Cuts at: 2502 2502
Size: 7042
SalI G'TCGA_C
Cuts at: 1936 4384 1936
Size: 2448 4594
SanDI GG'GwC_CC
Cuts at: 2067 2067
Size: 7042
SapI GCTCTTCn'nnn_
Cuts at: 4309 4309
Size: 7042
SbfI CC_TGCA'GG
Cuts at: 4394 4394
Size: 7042
ScaI AGT'ACT
Cuts at: 5290 7002 5290
Size: 1712 5330
SfcI C'TryA_G
Cuts at: 826 1045 3159 3864 4390 5525 6206 6397 826
Size: 219 2114 705 526 1135 681 191 1471
Fragments arranged by size:
2114 1471 1135 705 681 526 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7042
SmaI CCC'GGG
Cuts at: 1872 4369 1872
Size: 2497 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5146 6047 6309 820
Size: 197 926 3203 901 262 1553
Fragments arranged by size:
3203 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4833 493
Size: 4340 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7042
SphI G_CATG'C
Cuts at: 1132 2164 4400 1132
Size: 1032 2236 3774
Fragments arranged by size:
3774 2236 1032
SspI AAT'ATT
Cuts at: 5 52 2478 4966 5
Size: 47 2426 2488 2081
Fragments arranged by size:
2488 2426 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7042
StyI C'CwwG_G
Cuts at: 513 1065 2004 2533 4481 513
Size: 552 939 529 1948 3074
Fragments arranged by size:
3074 1948 939 552 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2686 5069 5086 5239 5424 5869 325
Size: 1255 1106 2383 17 153 185 445 1498
Fragments arranged by size:
2383 1498 1255 1106 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 3343 3642
Size: 274 80 33 51 1443 162 1204 299
Cuts at: 3642 5288 7000 96
Size: 1646 1712 138
Fragments arranged by size:
1712 1646 1443 1204 299 274 162 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 7002 7034 2224
Size: 32 4746 32 2232
Fragments arranged by size:
4746 2232 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7042
XbaI T'CTAG_A
Cuts at: 2926 4378 2926
Size: 1452 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7042
XmaI C'CCGG_G
Cuts at: 1870 4367 1870
Size: 2497 4545
XmnI GAAnn'nnTTC
Cuts at: 3548 4140 5171 3548
Size: 592 1031 5419
Fragments arranged by size:
5419 1031 592
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4850 276
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI XcmI