Restriction map for
pFN21AE9623
(Circular) (Six-base) MAPSORT of: pFN21AE9623.seq Check: 3669 from: 1 to: 6973
pFN21AE9623 6973 bp
With 182 enzymes: SgfI *
March 28, 2012 12:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3965 4783 278
Size: 53 83 186 936 2429 818 2468
Fragments arranged by size:
2468 2429 936 818 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4316 1937
Size: 2379 4594
Acc65I G'GTAC_C
Cuts at: 4294 4294
Size: 6973
AclI AA'CG_TT
Cuts at: 1563 5100 5473 1563
Size: 3537 373 3063
Fragments arranged by size:
3537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 2304 2663 2888 3356 3470 5036 6420
Size: 576 96 359 225 468 114 1566 1384
Cuts at: 6420 6688 1632
Size: 268 1917
Fragments arranged by size:
1917 1566 1384 576 468 359 268 225
114 96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6776
AflIII A'CryG_T
Cuts at: 1203 5872 6849 1203
Size: 4669 977 1327
Fragments arranged by size:
4669 1327 977
AgeI A'CCGG_T
Cuts at: 5804 5804
Size: 6973
AhdI GACnn_n'nnGTC
Cuts at: 5702 5702
Size: 6973
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2604 1184
Size: 1420 5553
AlwNI CAG_nnn'CTG
Cuts at: 3493 4716 6288 3493
Size: 1223 1572 4178
Fragments arranged by size:
4178 1572 1223
ApaLI G'TGCA_C
Cuts at: 5030 6186 5030
Size: 1156 5817
ApoI r'AATT_y
Cuts at: 1493 2363 2794 2917 3242 3841 3920 4282 4508
Size: 870 431 123 325 599 79 362 226
Cuts at: 4508 4660 6769 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 870 599 431 362 325 226
152 123 79
AseI AT'TA_AT
Cuts at: 160 1032 5527 160
Size: 872 4495 1606
Fragments arranged by size:
4495 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
AvaI C'yCGr_G
Cuts at: 1870 1943 4298 1870
Size: 73 2355 4545
Fragments arranged by size:
4545 2355 73
BamHI G'GATC_C
Cuts at: 2107 3587 4303 4670 2107
Size: 1480 716 367 4410
Fragments arranged by size:
4410 1480 716 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4294 5749 618
Size: 325 217 627 2507 1455 1842
Fragments arranged by size:
2507 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3098 4133 4292 1391
Size: 1707 1035 159 4072
Fragments arranged by size:
4072 1707 1035 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2819 928
Size: 971 920 5082
Fragments arranged by size:
5082 971 920
BbvCI CC'TCA_GC
Cuts at: 3161 3782 3161
Size: 621 6352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3815 3849 5164 5198 1903
Size: 34 1878 34 1315 34 3678
Fragments arranged by size:
3678 1878 1315 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3253 3784 4865 6075 3253
Size: 531 1081 1210 4151
Fragments arranged by size:
4151 1210 1081 531
BclI T'GATC_A
Cuts at: 1548 2179 1548
Size: 631 6342
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5584 136
Size: 107 122 71 631 605 3912 1525
Fragments arranged by size:
3912 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6968 6968
Size: 6973
BlpI GC'TnA_GC
Cuts at: 4390 4390
Size: 6973
Bme1580I G_kGCm'C
Cuts at: 1790 2600 5034 6190 1790
Size: 810 2434 1156 2573
Fragments arranged by size:
2573 2434 1156 810
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6973
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4704 5662 442
Size: 416 53 83 399 24 3287 958 1753
Fragments arranged by size:
3287 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3351 3351
Size: 6973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2693 2841 4038 5633 1239
Size: 1454 148 1197 1595 2579
Fragments arranged by size:
2579 1595 1454 1197 148
Bpu10I CC'TnA_GC
Cuts at: 3161 3782 3161
Size: 621 6352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4090 5098 5963 6261 4090
Size: 1008 865 298 4802
Fragments arranged by size:
4802 1008 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5636 882
Size: 617 4137 2219
Fragments arranged by size:
4137 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4764 493
Size: 711 3560 2702
Fragments arranged by size:
3560 2702 711
BsaBI GATnn'nnATC
Cuts at: 6967 6967
Size: 6973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3962 4780 5162 5797
Size: 53 83 186 936 2429 818 382 635
Cuts at: 5797 275
Size: 1451
Fragments arranged by size:
2429 1451 936 818 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5406 5804 6078 6225 6636 5406
Size: 398 274 147 411 5743
Fragments arranged by size:
5743 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6748
Fragments arranged by size:
6748 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3770 1186
Size: 2584 4389
BseYI C'CCAG_C
Cuts at: 2407 6176 6672 2407
Size: 3769 496 2708
Fragments arranged by size:
3769 2708 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3662 3759 1163
Size: 573 1926 97 4377
Fragments arranged by size:
4377 1926 573 97
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4423 5184 6212 1540
Size: 275 186 2422 761 1028 2301
Fragments arranged by size:
2422 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2943 4133 5034 5119 6190 2943
Size: 1190 901 85 1071 3726
Fragments arranged by size:
3726 1190 1071 901 85
BsmI GAATG_Cn'
Cuts at: 2700 3314 4484 4577 2700
Size: 614 1170 93 5096
Fragments arranged by size:
5096 1170 614 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2600 2943 3098 4133 4292 5034 5119
Size: 399 810 343 155 1035 159 742 85
Cuts at: 5119 6190 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1035 810 742 399 343 159
155 85
BspEI T'CCGG_A
Cuts at: 6636 6636
Size: 6973
BspHI T'CATG_A
Cuts at: 4862 6592 4862
Size: 1730 5243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4328 844
Size: 3484 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4860 1394
Size: 429 3037 3507
Fragments arranged by size:
3507 3037 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2520 2576 3067 3651 5468 5642 65
Size: 1183 1272 56 491 584 1817 174 1396
Fragments arranged by size:
1817 1396 1272 1183 584 491 174 56
BsrFI r'CCGG_y
Cuts at: 1700 1810 5617 5804 1700
Size: 110 3807 187 2869
Fragments arranged by size:
3807 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6973
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6973
BssSI C'ACGA_G
Cuts at: 5033 6045 5033
Size: 1012 5961
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4257 794
Size: 3463 3510
BstBI TT'CG_AA
Cuts at: 5777 5777
Size: 6973
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6973
BstXI CCAn_nnnn'nTGG
Cuts at: 3256 3256
Size: 6973
BstYI r'GATC_y
Cuts at: 1972 2107 3587 3762 4303 4670 5055 5072 6524
Size: 135 1480 175 541 367 385 17 1452
Cuts at: 6524 6610 6622 6968 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1480 1452 541 385 367 346 175
135 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6234
Fragments arranged by size:
6234 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 4489 5283 5303 2217
Size: 2272 794 20 3887
Fragments arranged by size:
3887 2272 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6938
DraI TTT'AAA
Cuts at: 4044 4277 4044
Size: 233 6740
DraIII CAC_nnn'GTG
Cuts at: 2357 2357
Size: 6973
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5980 809
Size: 507 4664 1802
Fragments arranged by size:
4664 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4420 5309 6938
Size: 54 1425 50 275 18 2590 889 1629
Cuts at: 6938 8
Size: 43
Fragments arranged by size:
2590 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4420 1537
Size: 275 2608 4090
Fragments arranged by size:
4090 2608 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3452 4903 1353
Size: 2099 1451 3423
Fragments arranged by size:
3423 2099 1451
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5560 5934 6080 387
Size: 1064 138 3971 374 146 1280
Fragments arranged by size:
3971 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4131 4131
Size: 6973
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2304 2663 2693 2841 2888 3356
Size: 393 576 96 359 30 148 47 468
Cuts at: 3356 3470 4038 5036 5633 6420 6688 1239
Size: 114 568 998 597 787 268 1524
Fragments arranged by size:
1524 998 787 597 576 568 468 393
359 268 148 114 96 47 30
EcoO109I rG'GnC_Cy
Cuts at: 2231 2849 2231
Size: 618 6355
EcoRI G'AATT_C
Cuts at: 2794 2917 4282 4660 2794
Size: 123 1365 378 5107
Fragments arranged by size:
5107 1365 378 123
EcoRV GAT'ATC
Cuts at: 3833 3833
Size: 6973
FspI TGC'GCA
Cuts at: 5479 5479
Size: 6973
HaeII r_GCGC'y
Cuts at: 1678 6120 1678
Size: 4442 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2866 2898 5614 5646 5688
Size: 32 135 32 1294 32 2716 32 42
Cuts at: 5688 5720 1373
Size: 32 2626
Fragments arranged by size:
2716 2626 1294 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4317 4563 669
Size: 988 281 2379 246 3079
Fragments arranged by size:
3079 2379 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2241 3040 748
Size: 1493 799 4681
Fragments arranged by size:
4681 1493 799
HpaI GTT'AAC
Cuts at: 4563 4563
Size: 6973
KpnI G_GTAC'C
Cuts at: 4298 4298
Size: 6973
MfeI C'AATT_G
Cuts at: 4572 4572
Size: 6973
MluI A'CGCG_T
Cuts at: 6849 6849
Size: 6973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6087 6271 1305
Size: 4782 184 2007
Fragments arranged by size:
4782 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5151
Fragments arranged by size:
5151 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 3542 4931 5290 5449 518
Size: 648 1009 1367 1389 359 159 2042
Fragments arranged by size:
2042 1389 1367 1009 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3811 4389 5066 6214 6459 1606
Size: 2205 578 677 1148 245 2120
Fragments arranged by size:
2205 2120 1148 677 578 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6421
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6973
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6973
NotI GC'GGCC_GC
Cuts at: 4420 4420
Size: 6973
NspI r_CATG'y
Cuts at: 1132 1229 2174 4331 5876 1132
Size: 97 945 2157 1545 2229
Fragments arranged by size:
2229 2157 1545 945 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6973
PciI A'CATG_T
Cuts at: 5872 5872
Size: 6973
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6973
PfoI T'CCnGG_A
Cuts at: 3255 3255
Size: 6973
PmeI GTTT'AAAC
Cuts at: 4277 4277
Size: 6973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5042 5074 6555 6587 5042
Size: 32 1481 32 5428
Fragments arranged by size:
5428 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2231 2849 2231
Size: 618 6355
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6973
PsiI TTA'TAA
Cuts at: 3729 4208 4543 3729
Size: 479 335 6159
Fragments arranged by size:
6159 479 335
PstI C_TGCA'G
Cuts at: 830 4325 830
Size: 3495 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6973
PvuII CAG'CTG
Cuts at: 3811 3811
Size: 6973
SacI G_AGCT'C
Cuts at: 4133 4133
Size: 6973
SalI G'TCGA_C
Cuts at: 1936 4315 1936
Size: 2379 4594
SapI GCTCTTCn'nnn_
Cuts at: 3452 3452
Size: 6973
SbfI CC_TGCA'GG
Cuts at: 4325 4325
Size: 6973
ScaI AGT'ACT
Cuts at: 5221 6933 5221
Size: 1712 5261
SfcI C'TryA_G
Cuts at: 826 1045 4094 4321 5456 6137 6328 826
Size: 219 3049 227 1135 681 191 1471
Fragments arranged by size:
3049 1471 1135 681 227 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6973
SmaI CCC'GGG
Cuts at: 1872 4300 1872
Size: 2428 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4069 5077 5978 6240 820
Size: 197 926 2126 1008 901 262 1553
Fragments arranged by size:
2126 1553 1008 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4764 493
Size: 4271 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6973
SphI G_CATG'C
Cuts at: 1132 4331 1132
Size: 3199 3774
SspI AAT'ATT
Cuts at: 5 52 2347 4897 5
Size: 47 2295 2550 2081
Fragments arranged by size:
2550 2295 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6973
StyI C'CwwG_G
Cuts at: 513 1065 2265 2484 4412 513
Size: 552 1200 219 1928 3074
Fragments arranged by size:
3074 1928 1200 552 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3348 3572 5000 5017 5170 5355 5800
Size: 1255 1768 224 1428 17 153 185 445
Cuts at: 5800 325
Size: 1498
Fragments arranged by size:
1768 1498 1428 1255 445 224 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5219 6931 96
Size: 274 80 33 51 1443 3242 1712 138
Fragments arranged by size:
3242 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6933 6965 6933
Size: 32 6941
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6973
XbaI T'CTAG_A
Cuts at: 3568 4309 3568
Size: 741 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6973
XmaI C'CCGG_G
Cuts at: 1870 4298 1870
Size: 2428 4545
XmnI GAAnn'nnTTC
Cuts at: 3033 5102 3033
Size: 2069 4904
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3963 4781 276
Size: 53 83 186 936 2429 818 2468
Fragments arranged by size:
2468 2429 936 818 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI