Restriction map for
pFN21AE9622
(Circular) (Six-base) MAPSORT of: pFN21AE9622.seq Check: 5417 from: 1 to: 6388
pFN21AE9622 6388 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3595 3595
Size: 6388
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2543 3731 1937
Size: 606 1188 4594
Fragments arranged by size:
4594 1188 606
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 2795 4515 4888 1563
Size: 1232 1720 373 3063
Fragments arranged by size:
3063 1720 1232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3117 3347 4451 5835 6103 1632
Size: 1485 230 1104 1384 268 1917
Fragments arranged by size:
1917 1485 1384 1104 268 230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 2282 5287 6264 1203
Size: 1079 3005 977 1327
Fragments arranged by size:
3005 1327 1079 977
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AlwNI CAG_nnn'CTG
Cuts at: 4131 5703 4131
Size: 1572 4816
ApaLI G'TGCA_C
Cuts at: 3436 4445 5601 3436
Size: 1009 1156 4223
Fragments arranged by size:
4223 1156 1009
ApoI r'AATT_y
Cuts at: 1493 2398 2424 2669 3520 3626 3697 3923 4075
Size: 905 26 245 851 106 71 226 152
Cuts at: 4075 6184 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 905 851 245 226 152 106
71 26
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 3713 1870
Size: 73 1770 4545
Fragments arranged by size:
4545 1770 73
BamHI G'GATC_C
Cuts at: 3718 4085 3718
Size: 367 6021
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3709 5164 618
Size: 325 217 627 1922 1455 1842
Fragments arranged by size:
1922 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3465 3574 3707 1391
Size: 2074 109 133 4072
Fragments arranged by size:
4072 2074 133 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 2820 3379 928
Size: 971 159 762 559 3937
Fragments arranged by size:
3937 971 762 559 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3363 4280 5490 3363
Size: 917 1210 4261
Fragments arranged by size:
4261 1210 917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BfrBI ATG'CAT
Cuts at: 2297 2297
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4999 136
Size: 107 122 71 631 605 3327 1525
Fragments arranged by size:
3327 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 3805 3805
Size: 6388
Bme1580I G_kGCm'C
Cuts at: 1790 3440 4449 5605 1790
Size: 1650 1009 1156 2573
Fragments arranged by size:
2573 1650 1156 1009
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2519 4119 5077
Size: 416 53 83 399 24 1102 1600 958
Cuts at: 5077 442
Size: 1753
Fragments arranged by size:
1753 1600 1102 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2377 3596 5048 1239
Size: 1138 1219 1452 2579
Fragments arranged by size:
2579 1452 1219 1138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2484 3703 4513 5378 5676 2484
Size: 1219 810 865 298 3196
Fragments arranged by size:
3196 1219 865 810 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5051 882
Size: 617 3552 2219
Fragments arranged by size:
3552 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4179 493
Size: 711 2975 2702
Fragments arranged by size:
2975 2702 711
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 4577 5212 275
Size: 53 83 186 936 2662 382 635 1451
Fragments arranged by size:
2662 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4821 5219 5493 5640 6051 4821
Size: 398 274 147 411 5158
Fragments arranged by size:
5158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3262 3292 1178
Size: 30 165 30 1859 30 4274
Fragments arranged by size:
4274 1859 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6388
BseYI C'CCAG_C
Cuts at: 3409 5591 6087 3409
Size: 2182 496 3710
Fragments arranged by size:
3710 2182 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 1163
Size: 573 376 5439
Fragments arranged by size:
5439 573 376
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3229 3440 4449 4534 5605 3229
Size: 211 1009 85 1071 4012
Fragments arranged by size:
4012 1071 1009 211 85
BsmI GAATG_Cn'
Cuts at: 3899 3992 3899
Size: 93 6295
BsmBI CGTCTCn'nnnn_
Cuts at: 3245 3547 3245
Size: 302 6086
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3229 3440 3465 3574 3707 4449 4534
Size: 399 1439 211 25 109 133 742 85
Cuts at: 4534 5605 1391
Size: 1071 2174
Fragments arranged by size:
2174 1439 1071 742 399 211 133 109
85 25
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2344 3595 3743 844
Size: 1259 241 1251 148 3489
Fragments arranged by size:
3489 1259 1251 241 148
BsrBI CCG'CTC
Cuts at: 1394 1823 4275 1394
Size: 429 2452 3507
Fragments arranged by size:
3507 2452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3070 4883 5057 65
Size: 1183 1822 1813 174 1396
Fragments arranged by size:
1822 1813 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5032 5219 1700
Size: 110 3222 187 2869
Fragments arranged by size:
3222 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2725 96
Size: 2629 3759
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 3181 4448 5460 3181
Size: 1267 1012 4109
Fragments arranged by size:
4109 1267 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6388
BstBI TT'CG_AA
Cuts at: 2673 3476 5192 2673
Size: 803 1716 3869
Fragments arranged by size:
3869 1716 803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstXI CCAn_nnnn'nTGG
Cuts at: 2650 2650
Size: 6388
BstYI r'GATC_y
Cuts at: 1972 3718 4085 4470 4487 5939 6025 6037 6383
Size: 1746 367 385 17 1452 86 12 346
Cuts at: 6383 1972
Size: 1977
Fragments arranged by size:
1977 1746 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4897
Fragments arranged by size:
4897 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 2159 3420 3904 4698 4718 2159
Size: 1261 484 794 20 3829
Fragments arranged by size:
3829 1261 794 484 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 2589 3692 2589
Size: 1103 5285
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3835 4724
Size: 54 1425 50 275 18 177 1828 889
Cuts at: 4724 6353 8
Size: 1629 43
Fragments arranged by size:
1828 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2877 3018 3481 4318 1353
Size: 1524 141 463 837 3423
Fragments arranged by size:
3423 1524 837 463 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4975 5349 5495 387
Size: 1064 138 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2377 3117 3347 3596 4451 5048 5835
Size: 393 745 740 230 249 855 597 787
Cuts at: 5835 6103 1239
Size: 268 1524
Fragments arranged by size:
1524 855 787 745 740 597 393 268
249 230
EcoO109I rG'GnC_Cy
Cuts at: 3493 3493
Size: 6388
EcoRI G'AATT_C
Cuts at: 2669 3520 3697 4075 2669
Size: 851 177 378 4982
Fragments arranged by size:
4982 851 378 177
EcoRV GAT'ATC
Cuts at: 2245 3334 2245
Size: 1089 5299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2063 2327 2063
Size: 264 6124
FspI TGC'GCA
Cuts at: 4894 4894
Size: 6388
HaeII r_GCGC'y
Cuts at: 1678 5535 1678
Size: 3857 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 5029 5061 5103
Size: 32 135 32 803 32 2622 32 42
Cuts at: 5103 5135 1373
Size: 32 2626
Fragments arranged by size:
2626 2622 803 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2764 3732 3978 669
Size: 988 281 826 968 246 3079
Fragments arranged by size:
3079 988 968 826 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 2764 3978 2764
Size: 1214 5174
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2662 3073 5502 5686 1305
Size: 1357 411 2429 184 2007
Fragments arranged by size:
2429 2007 1357 411 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4389
Fragments arranged by size:
4389 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4346 4705 4864 518
Size: 648 3180 359 159 2042
Fragments arranged by size:
3180 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2257 3804 4481 5629 5874 1606
Size: 651 1547 677 1148 245 2120
Fragments arranged by size:
2120 1547 1148 677 651 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4897
Fragments arranged by size:
4897 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NsiI A_TGCA'T
Cuts at: 2299 2299
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 2286 2710 3746 5291 1132
Size: 97 1057 424 1036 1545 2229
Fragments arranged by size:
2229 1545 1057 1036 424 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6388
PciI A'CATG_T
Cuts at: 2282 5287 2282
Size: 3005 3383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6388
PfoI T'CCnGG_A
Cuts at: 2449 2449
Size: 6388
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3493 3493
Size: 6388
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PstI C_TGCA'G
Cuts at: 830 3740 830
Size: 2910 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2257 2257
Size: 6388
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SanDI GG'GwC_CC
Cuts at: 3493 3493
Size: 6388
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2252 3649 3736 4871 5552 5743 826
Size: 219 1207 1397 87 1135 681 191 1471
Fragments arranged by size:
1471 1397 1207 1135 681 219 191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2499 3682 4492 5393 5655 820
Size: 197 926 556 1183 810 901 262 1553
Fragments arranged by size:
1553 1183 926 901 810 556 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 2698 152
Size: 2546 3842
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 4312 5
Size: 47 4260 2081
Fragments arranged by size:
4260 2081 47
StuI AGG'CCT
Cuts at: 1517 3211 1517
Size: 1694 4694
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 3827 513
Size: 552 939 6 1817 3074
Fragments arranged by size:
3074 1817 939 552 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4415 4432 4585 4770 5215 325
Size: 1255 2835 17 153 185 445 1498
Fragments arranged by size:
2835 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2725 3165 4634
Size: 274 80 33 51 1443 748 440 1469
Cuts at: 4634 6346 96
Size: 1712 138
Fragments arranged by size:
1712 1469 1443 748 440 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6348 6380 6348
Size: 32 6356
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6388
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 2466 3376 4517 2466
Size: 910 1141 4337
Fragments arranged by size:
4337 1141 910
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI