Restriction map for
pFN21AE9621
(Circular) (Six-base) MAPSORT of: pFN21AE9621.seq Check: 5505 from: 1 to: 6373
pFN21AE9621 6373 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4183 278
Size: 53 83 186 936 2647 2468
Fragments arranged by size:
2647 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3716 1937
Size: 1779 4594
Acc65I G'GTAC_C
Cuts at: 3694 3694
Size: 6373
AclI AA'CG_TT
Cuts at: 1563 4500 4873 1563
Size: 2937 373 3063
Fragments arranged by size:
3063 2937 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2841 3449 4436 5820 6088 1632
Size: 522 687 608 987 1384 268 1917
Fragments arranged by size:
1917 1384 987 687 608 522 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6176
AflIII A'CryG_T
Cuts at: 1203 2017 5272 6249 1203
Size: 814 3255 977 1327
Fragments arranged by size:
3255 1327 977 814
AgeI A'CCGG_T
Cuts at: 5204 5204
Size: 6373
AhdI GACnn_n'nnGTC
Cuts at: 5102 5102
Size: 6373
AleI CACnn'nnGTG
Cuts at: 1166 2018 1166
Size: 852 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6373
AlwNI CAG_nnn'CTG
Cuts at: 4116 5688 4116
Size: 1572 4801
ApaLI G'TGCA_C
Cuts at: 4430 5586 4430
Size: 1156 5217
ApoI r'AATT_y
Cuts at: 1493 2133 2302 2342 2440 2493 2536 2709 3566
Size: 640 169 40 98 53 43 173 857
Cuts at: 3566 3682 3908 4060 6169 1493
Size: 116 226 152 2109 1697
Fragments arranged by size:
2109 1697 857 640 226 173 169 152
116 98 53 43 40
AseI AT'TA_AT
Cuts at: 160 1032 4927 160
Size: 872 3895 1606
Fragments arranged by size:
3895 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
AvaI C'yCGr_G
Cuts at: 1870 1943 3698 1870
Size: 73 1755 4545
Fragments arranged by size:
4545 1755 73
BamHI G'GATC_C
Cuts at: 3703 4070 3703
Size: 367 6006
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3694 5149 618
Size: 325 217 627 1907 1455 1842
Fragments arranged by size:
1907 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2922 3692 1391
Size: 1531 770 4072
Fragments arranged by size:
4072 1531 770
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 2694 928
Size: 971 610 185 4607
Fragments arranged by size:
4607 971 610 185
BbvCI CC'TCA_GC
Cuts at: 3345 3345
Size: 6373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4564 4598 1903
Size: 34 2627 34 3678
Fragments arranged by size:
3678 2627 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3280 4265 5475 3280
Size: 985 1210 4178
Fragments arranged by size:
4178 1210 985
BclI T'GATC_A
Cuts at: 1548 2668 1548
Size: 1120 5253
BfrBI ATG'CAT
Cuts at: 2695 2695
Size: 6373
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4984 136
Size: 107 122 71 631 605 3312 1525
Fragments arranged by size:
3312 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6368 6368
Size: 6373
BlpI GC'TnA_GC
Cuts at: 3611 3790 3611
Size: 179 6194
Bme1580I G_kGCm'C
Cuts at: 1790 4434 5590 1790
Size: 2644 1156 2573
Fragments arranged by size:
2644 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6373
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 4104 5062
Size: 416 53 83 399 24 663 2024 958
Cuts at: 5062 442
Size: 1753
Fragments arranged by size:
2024 1753 958 663 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2601 5033 1239
Size: 1362 2432 2579
Fragments arranged by size:
2579 2432 1362
Bpu10I CC'TnA_GC
Cuts at: 3345 3345
Size: 6373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4498 5363 5661 4498
Size: 865 298 5210
Fragments arranged by size:
5210 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5036 882
Size: 617 3537 2219
Fragments arranged by size:
3537 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4164 493
Size: 711 2960 2702
Fragments arranged by size:
2960 2702 711
BsaBI GATnn'nnATC
Cuts at: 6367 6367
Size: 6373
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4180 4562 5197 275
Size: 53 83 186 936 2647 382 635 1451
Fragments arranged by size:
2647 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4806 5204 5478 5625 6036 4806
Size: 398 274 147 411 5143
Fragments arranged by size:
5143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6148
Fragments arranged by size:
6148 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2905 3042 1186
Size: 1719 137 4517
Fragments arranged by size:
4517 1719 137
BseYI C'CCAG_C
Cuts at: 5576 6072 5576
Size: 496 5877
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5800
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3823 4584 5612 1540
Size: 275 186 1822 761 1028 2301
Fragments arranged by size:
2301 1822 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2922 3625 4434 4519 5590 2922
Size: 703 809 85 1071 3705
Fragments arranged by size:
3705 1071 809 703 85
BsmI GAATG_Cn'
Cuts at: 2377 3510 3884 3977 2377
Size: 1133 374 93 4773
Fragments arranged by size:
4773 1133 374 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2922 3625 3692 4434 4519 5590 1391
Size: 399 1132 703 67 742 85 1071 2174
Fragments arranged by size:
2174 1132 1071 742 703 399 85 67
BspEI T'CCGG_A
Cuts at: 6036 6036
Size: 6373
BspHI T'CATG_A
Cuts at: 2347 3280 4262 5992 2347
Size: 933 982 1730 2728
Fragments arranged by size:
2728 1730 982 933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3728 844
Size: 2884 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4260 1394
Size: 429 2437 3507
Fragments arranged by size:
3507 2437 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2330 2862 3427 4868 5042 65
Size: 1183 1082 532 565 1441 174 1396
Fragments arranged by size:
1441 1396 1183 1082 565 532 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5017 5204 1700
Size: 110 3207 187 2869
Fragments arranged by size:
3207 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6373
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6373
BssSI C'ACGA_G
Cuts at: 4433 5445 4433
Size: 1012 5361
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6373
BstBI TT'CG_AA
Cuts at: 2164 2197 5177 2164
Size: 33 2980 3360
Fragments arranged by size:
3360 2980 33
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6373
BstYI r'GATC_y
Cuts at: 1972 2905 3263 3703 4070 4455 4472 5924 6010
Size: 933 358 440 367 385 17 1452 86
Cuts at: 6010 6022 6368 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 933 440 385 367 358 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5821
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5634
Fragments arranged by size:
5634 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3889 4683 4703 2245
Size: 1644 794 20 3915
Fragments arranged by size:
3915 1644 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6373
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6338
DraI TTT'AAA
Cuts at: 3121 3677 3121
Size: 556 5817
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5380 809
Size: 507 4064 1802
Fragments arranged by size:
4064 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3820 4709
Size: 54 1425 50 275 18 216 1774 889
Cuts at: 4709 6338 8
Size: 1629 43
Fragments arranged by size:
1774 1629 1425 889 275 216 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3820 1537
Size: 275 2008 4090
Fragments arranged by size:
4090 2008 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2817 3014 4303 1353
Size: 1464 197 1289 3423
Fragments arranged by size:
3423 1464 1289 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4960 5334 5480 387
Size: 1064 138 3371 374 146 1280
Fragments arranged by size:
3371 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2920 2920
Size: 6373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2601 2841 3449 4436 5033 5820
Size: 393 522 447 240 608 987 597 787
Cuts at: 5820 6088 1239
Size: 268 1524
Fragments arranged by size:
1524 987 787 608 597 522 447 393
268 240
EcoRI G'AATT_C
Cuts at: 2536 3682 4060 2536
Size: 1146 378 4849
Fragments arranged by size:
4849 1146 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2267 2267
Size: 6373
FspI TGC'GCA
Cuts at: 4879 4879
Size: 6373
HaeII r_GCGC'y
Cuts at: 1678 5520 1678
Size: 3842 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2638 2670 3338 3370 3460
Size: 32 135 32 1066 32 668 32 90
Cuts at: 3460 3492 5014 5046 5088 5120 1373
Size: 32 1522 32 42 32 2626
Fragments arranged by size:
2626 1522 1066 668 135 90 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3048 3717 3963 669
Size: 988 281 349 761 669 246 3079
Fragments arranged by size:
3079 988 761 669 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6373
HpaI GTT'AAC
Cuts at: 3963 3963
Size: 6373
KpnI G_GTAC'C
Cuts at: 3698 3698
Size: 6373
MfeI C'AATT_G
Cuts at: 2847 3972 2847
Size: 1125 5248
MluI A'CGCG_T
Cuts at: 6249 6249
Size: 6373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5487 5671 1305
Size: 4182 184 2007
Fragments arranged by size:
4182 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 10
Size: 54 1425 343 216 4335
Fragments arranged by size:
4335 1425 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2969 4331 4690 4849 518
Size: 648 852 951 1362 359 159 2042
Fragments arranged by size:
2042 1362 951 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3560 3598 3789 4466 5614 5859 1606
Size: 1954 38 191 677 1148 245 2120
Fragments arranged by size:
2120 1954 1148 677 245 191 38
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5821
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6373
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6373
NotI GC'GGCC_GC
Cuts at: 3820 3820
Size: 6373
NsiI A_TGCA'T
Cuts at: 2697 2697
Size: 6373
NspI r_CATG'y
Cuts at: 1132 1229 2021 2695 3731 5276 1132
Size: 97 792 674 1036 1545 2229
Fragments arranged by size:
2229 1545 1036 792 674 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6373
PciI A'CATG_T
Cuts at: 2017 5272 2017
Size: 3255 3118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2055 1108
Size: 947 5426
PmeI GTTT'AAAC
Cuts at: 3677 3677
Size: 6373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4442 4474 5955 5987 4442
Size: 32 1481 32 4828
Fragments arranged by size:
4828 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6373
PsiI TTA'TAA
Cuts at: 3666 3943 3666
Size: 277 6096
PstI C_TGCA'G
Cuts at: 830 2638 3725 830
Size: 1808 1087 3478
Fragments arranged by size:
3478 1808 1087
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6373
PvuII CAG'CTG
Cuts at: 3560 3598 3560
Size: 38 6335
SacI G_AGCT'C
Cuts at: 2922 2922
Size: 6373
SalI G'TCGA_C
Cuts at: 1936 3715 1936
Size: 1779 4594
SbfI CC_TGCA'GG
Cuts at: 3725 3725
Size: 6373
ScaI AGT'ACT
Cuts at: 4621 6333 4621
Size: 1712 4661
SfcI C'TryA_G
Cuts at: 826 1045 2634 3532 3721 4856 5537 5728 826
Size: 219 1589 898 189 1135 681 191 1471
Fragments arranged by size:
1589 1471 1135 898 681 219 191 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6373
SmaI CCC'GGG
Cuts at: 1872 3700 1872
Size: 1828 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4477 5378 5640 820
Size: 197 926 2534 901 262 1553
Fragments arranged by size:
2534 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4164 493
Size: 3671 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6373
SphI G_CATG'C
Cuts at: 1132 3731 1132
Size: 2599 3774
SspI AAT'ATT
Cuts at: 5 52 2808 3301 3433 4297 5
Size: 47 2756 493 132 864 2081
Fragments arranged by size:
2756 2081 864 493 132 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6373
StyI C'CwwG_G
Cuts at: 513 1065 2145 3812 513
Size: 552 1080 1667 3074
Fragments arranged by size:
3074 1667 1080 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 2375 3561 4400 4417 4570 4755
Size: 1255 578 217 1186 839 17 153 185
Cuts at: 4755 5200 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1186 839 578 445 217 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4619 6331 96
Size: 274 80 33 51 1443 2642 1712 138
Fragments arranged by size:
2642 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6333 6365 6333
Size: 32 6341
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6373
XbaI T'CTAG_A
Cuts at: 2113 3709 2113
Size: 1596 4777
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6373
XmaI C'CCGG_G
Cuts at: 1870 3698 1870
Size: 1828 4545
XmnI GAAnn'nnTTC
Cuts at: 2133 2247 2343 4502 2133
Size: 114 96 2159 4004
Fragments arranged by size:
4004 2159 114 96
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4181 276
Size: 53 83 186 936 2647 2468
Fragments arranged by size:
2647 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI