Restriction map for
pFN21AE9619
(Circular) (Six-base) MAPSORT of: pFN21AE9619.seq Check: 3613 from: 1 to: 5959
pFN21AE9619 5959 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2499 4022 5406 5674 1632
Size: 867 1523 1384 268 1917
Fragments arranged by size:
1917 1523 1384 867 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 2130 4858 5835 1203
Size: 927 2728 977 1327
Fragments arranged by size:
2728 1327 977 927
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 3702 5274 3702
Size: 1572 4387
ApaLI G'TGCA_C
Cuts at: 2708 4016 5172 2708
Size: 1308 1156 3495
Fragments arranged by size:
3495 1308 1156
ApoI r'AATT_y
Cuts at: 1493 2261 2774 3268 3494 3646 5755 1493
Size: 768 513 494 226 152 2109 1697
Fragments arranged by size:
2109 1697 768 513 494 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3278 1391
Size: 1887 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2505 3042 928
Size: 971 606 537 3845
Fragments arranged by size:
3845 971 606 537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2578 2712 4020 5176 1790
Size: 788 134 1308 1156 2573
Fragments arranged by size:
2573 1308 1156 788 134
BmgBI CAC'GTC
Cuts at: 1318 2131 1318
Size: 813 5146
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 4619 1239
Size: 1047 2333 2579
Fragments arranged by size:
2579 2333 1047
Bpu10I CC'TnA_GC
Cuts at: 2399 2399
Size: 5959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2146 3108 4084 4949 5247 2146
Size: 962 976 865 298 2858
Fragments arranged by size:
2858 976 962 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2204 4622 882
Size: 617 705 2418 2219
Fragments arranged by size:
2418 2219 705 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2289 1186
Size: 1103 4856
BseYI C'CCAG_C
Cuts at: 2176 2577 5162 5658 2176
Size: 401 2585 496 2477
Fragments arranged by size:
2585 2477 496 401
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 3409 4170 5198 1540
Size: 275 186 29 1379 761 1028 2301
Fragments arranged by size:
2301 1379 1028 761 275 186 29
BsiHKAI G_wGCw'C
Cuts at: 2712 4020 4105 5176 2712
Size: 1308 85 1071 3495
Fragments arranged by size:
3495 1308 1071 85
BsmI GAATG_Cn'
Cuts at: 2926 3067 3470 3563 2926
Size: 141 403 93 5322
Fragments arranged by size:
5322 403 141 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2578 2712 3278 4020 4105 5176 1391
Size: 399 788 134 566 742 85 1071 2174
Fragments arranged by size:
2174 1071 788 742 566 399 134 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2488 2650 3848 5578 2488
Size: 162 1198 1730 2869
Fragments arranged by size:
2869 1730 1198 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 2500 4763 2500
Size: 2263 3696
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954
Size: 1317 367 385 17 1452 86 12 346
Cuts at: 5954 1972
Size: 1977
Fragments arranged by size:
1977 1452 1317 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3056 3056
Size: 5959
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2030 513
Size: 552 939 8 18 4442
Fragments arranged by size:
4442 939 552 18 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2821 487
Size: 683 56 1595 3625
Fragments arranged by size:
3625 1595 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2257 2292 529
Size: 35 1693 35 4196
Fragments arranged by size:
4196 1693 35 35
DraI TTT'AAA
Cuts at: 3087 3181 3263 3087
Size: 94 82 5783
Fragments arranged by size:
5783 94 82
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 3406 4295
Size: 54 1425 50 275 18 197 1379 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1379 889 275 197 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2027 3406 1537
Size: 275 215 1379 4090
Fragments arranged by size:
4090 1379 275 215
EarI CTCTTCn'nnn_
Cuts at: 1353 2296 3889 1353
Size: 943 1593 3423
Fragments arranged by size:
3423 1593 943
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2499 4022 4619 5406 5674 1239
Size: 393 654 213 1523 597 787 268 1524
Fragments arranged by size:
1524 1523 787 654 597 393 268 213
EcoNI CCTnn'n_nnAGG
Cuts at: 3251 3251
Size: 5959
EcoO109I rG'GnC_Cy
Cuts at: 2564 2564
Size: 5959
EcoRI G'AATT_C
Cuts at: 2261 3268 3646 2261
Size: 1007 378 4574
Fragments arranged by size:
4574 1007 378
EcoRV GAT'ATC
Cuts at: 2646 2941 2646
Size: 295 5664
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2203 2235 2271 2303 3032
Size: 32 135 32 631 32 36 32 729
Cuts at: 3032 3064 4600 4632 4674 4706 1373
Size: 32 1536 32 42 32 2626
Fragments arranged by size:
2626 1536 729 631 135 42 36 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3303 3549 669
Size: 988 281 963 402 246 3079
Fragments arranged by size:
3079 988 963 402 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 3917 4276 4435 518
Size: 648 1710 1041 359 159 2042
Fragments arranged by size:
2042 1710 1041 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2032 2537 3004 3375 4052 5200 5445
Size: 408 18 505 467 371 677 1148 245
Cuts at: 5445 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 505 467 408 371 245
18
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4468
Fragments arranged by size:
4468 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 2027 3406 2027
Size: 1379 4580
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2302 2973 2302
Size: 671 5288
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2564 2564
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 3311 830
Size: 2481 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2537 3004 2537
Size: 467 5492
SacII CC_GC'GG
Cuts at: 2015 2033 2015
Size: 18 5941
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 3307 4442 5123 5314 826
Size: 219 2262 1135 681 191 1471
Fragments arranged by size:
2262 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2161 3123 4063 4964 5226 820
Size: 197 926 218 962 940 901 262 1553
Fragments arranged by size:
1553 962 940 926 901 262 218 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 2761 3883 5
Size: 47 2709 1122 2081
Fragments arranged by size:
2709 2081 1122 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2004 3398 513
Size: 552 939 1394 3074
Fragments arranged by size:
3074 1394 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 3986 4003 4156 4341 4786 325
Size: 1255 588 1818 17 153 185 445 1498
Fragments arranged by size:
1818 1498 1255 588 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2477 4205 5917
Size: 274 80 33 51 1443 500 1728 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1728 1712 1443 500 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 2086 2985 3049 4088 2086
Size: 899 64 1039 3957
Fragments arranged by size:
3957 1039 899 64
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI