Restriction map for
pFN21AE9618
(Circular) (Six-base) MAPSORT of: pFN21AE9618.seq Check: 5254 from: 1 to: 5902
pFN21AE9618 5902 bp
With 182 enzymes: SgfI *
March 28, 2012 12:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2777 2777
Size: 5902
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2458 3965 5349 5617 1632
Size: 826 1507 1384 268 1917
Fragments arranged by size:
1917 1507 1384 826 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 2412 3645 5217 2412
Size: 1233 1572 3097
Fragments arranged by size:
3097 1572 1233
ApaI G_GGCC'C
Cuts at: 2173 3054 2173
Size: 881 5021
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 3211 3437 3589 5698 1493
Size: 1718 226 152 2109 1697
Fragments arranged by size:
2109 1718 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2023 2195 2023
Size: 172 5730
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 2531 3227 1870
Size: 73 279 309 696 4545
Fragments arranged by size:
4545 696 309 279 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 3223 4678 618
Size: 325 217 627 440 996 1455 1842
Fragments arranged by size:
1842 1455 996 627 440 325 217
BanII G_rGCy'C
Cuts at: 1391 2173 2184 3014 3054 3221 1391
Size: 782 11 830 40 167 4072
Fragments arranged by size:
4072 830 782 167 40 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2126 2160 4093 4127 1903
Size: 34 189 34 1933 34 3678
Fragments arranged by size:
3678 1933 189 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2941 5897 2941
Size: 2956 2946
BlpI GC'TnA_GC
Cuts at: 2391 3319 2391
Size: 928 4974
Bme1580I G_kGCm'C
Cuts at: 1790 2173 2232 3054 3963 5119 1790
Size: 383 59 822 909 1156 2573
Fragments arranged by size:
2573 1156 909 822 383 59
BmgBI CAC'GTC
Cuts at: 1318 2818 3082 3100 1318
Size: 1500 264 18 4120
Fragments arranged by size:
4120 1500 264 18
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2262 2451 3186
Size: 416 53 83 399 24 845 189 735
Cuts at: 3186 3633 4591 442
Size: 447 958 1753
Fragments arranged by size:
1753 958 845 735 447 416 399 189
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2990 4562 1239
Size: 1253 498 1572 2579
Fragments arranged by size:
2579 1572 1253 498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2723 4027 4892 5190 2723
Size: 1304 865 298 3435
Fragments arranged by size:
3435 1304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3693 493
Size: 711 2489 2702
Fragments arranged by size:
2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2241 4335 4733 5007 5154 5565 2241
Size: 2094 398 274 147 411 2578
Fragments arranged by size:
2578 2094 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5902
BseYI C'CCAG_C
Cuts at: 3087 3091 5105 5601 3087
Size: 4 2014 496 3388
Fragments arranged by size:
3388 2014 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2755 2836 3185 1163
Size: 573 1019 81 349 3880
Fragments arranged by size:
3880 1019 573 349 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3014 3963 4048 5119 3014
Size: 949 85 1071 3797
Fragments arranged by size:
3797 1071 949 85
BsmI GAATG_Cn'
Cuts at: 2237 2463 3413 3506 2237
Size: 226 950 93 4633
Fragments arranged by size:
4633 950 226 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2173 2184 2232 3014 3054 3221 3963
Size: 399 383 11 48 782 40 167 742
Cuts at: 3963 4048 5119 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 782 742 399 383 167 85
48 40 11
BspEI T'CCGG_A
Cuts at: 2241 5565 2241
Size: 3324 2578
BspHI T'CATG_A
Cuts at: 2653 3791 5521 2653
Size: 1138 1730 3034
Fragments arranged by size:
3034 1730 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 3257 844
Size: 1933 480 3489
Fragments arranged by size:
3489 1933 480
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2441 2855 4546 4733 1700
Size: 110 631 414 1691 187 2869
Fragments arranged by size:
2869 1691 631 414 187 110
BsrGI T'GTAC_A
Cuts at: 96 2496 96
Size: 2400 3502
BssHII G'CGCG_C
Cuts at: 1925 2023 2195 1925
Size: 98 172 5632
Fragments arranged by size:
5632 172 98
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2778 794
Size: 1984 3918
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2941 3232 3599 3984 4001 5453 5539 5551
Size: 969 291 367 385 17 1452 86 12
Cuts at: 5551 5897 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 969 385 367 346 291 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2164 2173 2934 513
Size: 552 939 24 136 9 761 3481
Fragments arranged by size:
3481 939 761 552 136 24 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2362 3418 4212 4232 2362
Size: 1056 794 20 4032
Fragments arranged by size:
4032 1056 794 20
ClaI AT'CG_AT
Cuts at: 1554 2694 1554
Size: 1140 4762
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DraIII CAC_nnn'GTG
Cuts at: 2459 3112 2459
Size: 653 5249
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2589 3349 4238
Size: 54 1425 50 275 18 759 760 889
Cuts at: 4238 5867 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 760 759 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3141 3832 1353
Size: 1788 691 3423
Fragments arranged by size:
3423 1788 691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3012 3012
Size: 5902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2458 2492 2990 3965 4562 5349 5617
Size: 393 826 34 498 975 597 787 268
Cuts at: 5617 1239
Size: 1524
Fragments arranged by size:
1524 975 826 787 597 498 393 268
34
EcoNI CCTnn'n_nnAGG
Cuts at: 2206 2416 2206
Size: 210 5692
EcoO109I rG'GnC_Cy
Cuts at: 2169 2961 3050 3051 3157 2169
Size: 792 89 1 106 4914
Fragments arranged by size:
4914 792 106 89 1
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 5049 1678
Size: 3371 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 2696 2728 3015
Size: 32 135 32 890 32 202 32 287
Cuts at: 3015 3047 4543 4575 4617 4649 1373
Size: 32 1496 32 42 32 2626
Fragments arranged by size:
2626 1496 890 287 202 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2369 3182 748
Size: 1621 813 3468
Fragments arranged by size:
3468 1621 813
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2320 5016 5200 1305
Size: 1015 2696 184 2007
Fragments arranged by size:
2696 2007 1015 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 10
Size: 54 1425 343 759 3321
Fragments arranged by size:
3321 1425 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2175 2841 2896 3091 3169 3318 3995
Size: 424 145 666 55 195 78 149 677
Cuts at: 3995 5143 5388 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 424 245 195 149
145 78 55
NaeI GCC'GGC
Cuts at: 2443 2443
Size: 5902
NcoI C'CATG_G
Cuts at: 513 1065 2004 2164 2934 513
Size: 552 939 160 770 3481
Fragments arranged by size:
3481 939 770 552 160
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NgoMIV G'CCGG_C
Cuts at: 2441 2441
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2921 3260 4805 1132
Size: 97 1692 339 1545 2229
Fragments arranged by size:
2229 1692 1545 339 97
PasI CC'CwG_GG
Cuts at: 1348 2232 2795 1348
Size: 884 563 4455
Fragments arranged by size:
4455 884 563
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2422 1108
Size: 1314 4588
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2961 3157 2961
Size: 196 5706
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PspOMI G'GGCC_C
Cuts at: 2169 3050 2169
Size: 881 5021
PstI C_TGCA'G
Cuts at: 830 2382 3254 830
Size: 1552 872 3478
Fragments arranged by size:
3478 1552 872
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 3091 3091
Size: 5902
SacI G_AGCT'C
Cuts at: 3014 3014
Size: 5902
SacII CC_GC'GG
Cuts at: 2031 2176 2031
Size: 145 5757
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 2382 3254 2382
Size: 872 5030
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2378 3250 4385 5066 5257 826
Size: 219 1333 872 1135 681 191 1471
Fragments arranged by size:
1471 1333 1135 872 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 2855 1700
Size: 1155 4747
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2702 4006 4907 5169 820
Size: 197 926 759 1304 901 262 1553
Fragments arranged by size:
1553 1304 926 901 759 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 2102 2212 1517
Size: 585 110 5207
Fragments arranged by size:
5207 585 110
StyI C'CwwG_G
Cuts at: 513 1065 2004 2164 2184 2592 2934 2956 3054
Size: 552 939 160 20 408 342 22 98
Cuts at: 3054 3341 513
Size: 287 3074
Fragments arranged by size:
3074 939 552 408 342 287 160 98
22 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2312 2470 3011 3929 3946 4099 4284
Size: 1255 732 158 541 918 17 153 185
Cuts at: 4284 4729 325
Size: 445 1498
Fragments arranged by size:
1498 1255 918 732 541 445 185 158
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 4148 5860
Size: 274 80 33 51 1443 519 1652 1712
Cuts at: 5860 96
Size: 138
Fragments arranged by size:
1712 1652 1443 519 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI