Restriction map for
pFN21AE9616
(Circular) (Six-base) MAPSORT of: pFN21AE9616.seq Check: 1634 from: 1 to: 5773
pFN21AE9616 5773 bp
With 182 enzymes: SgfI *
March 28, 2012 12:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3046 3046
Size: 5773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1995 2207 2464 2541 3005 3836 5220 5488
Size: 363 212 257 77 464 831 1384 268
Cuts at: 5488 1632
Size: 1917
Fragments arranged by size:
1917 1384 831 464 363 268 257 212
77
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 3516 5088 3516
Size: 1572 4201
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 2160 3082 3308 3460 5569 1493
Size: 667 922 226 152 2109 1697
Fragments arranged by size:
2109 1697 922 667 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 3098 1870
Size: 73 1155 4545
Fragments arranged by size:
4545 1155 73
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3094 4549 618
Size: 325 217 627 1307 1455 1842
Fragments arranged by size:
1842 1455 1307 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2364 3092 1391
Size: 973 728 4072
Fragments arranged by size:
4072 973 728
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2781 928
Size: 971 882 3920
Fragments arranged by size:
3920 971 882
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 2068 1548
Size: 520 5253
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4384 136
Size: 107 122 71 631 605 2712 1525
Fragments arranged by size:
2712 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 2363 3190 2363
Size: 827 4946
Bme1580I G_kGCm'C
Cuts at: 1790 3834 4990 1790
Size: 2044 1156 2573
Fragments arranged by size:
2573 2044 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2929 3504 4462
Size: 416 53 83 399 24 1512 575 958
Cuts at: 4462 442
Size: 1753
Fragments arranged by size:
1753 1512 958 575 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 4433 1239
Size: 911 2283 2579
Fragments arranged by size:
2579 2283 911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2235 3898 4763 5061 2235
Size: 1663 865 298 2947
Fragments arranged by size:
2947 1663 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 882
Size: 617 2937 2219
Fragments arranged by size:
2937 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3564 493
Size: 711 2360 2702
Fragments arranged by size:
2702 2360 711
BsaBI GATnn'nnATC
Cuts at: 3055 3061 5767 3055
Size: 6 2706 3061
Fragments arranged by size:
3061 2706 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3580 3962 4597 275
Size: 53 83 186 936 2047 382 635 1451
Fragments arranged by size:
2047 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4206 4604 4878 5025 5436 4206
Size: 398 274 147 411 4543
Fragments arranged by size:
4543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5548
Fragments arranged by size:
5548 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2238 1186
Size: 1052 4721
BseYI C'CCAG_C
Cuts at: 4976 5472 4976
Size: 496 5277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5200
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 3834 3919 4990 2364
Size: 1470 85 1071 3147
Fragments arranged by size:
3147 1470 1071 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 3092 3834 3919 4990 1391
Size: 399 574 728 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 728 574 399 85
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 2065 3662 5392 2065
Size: 1597 1730 2446
Fragments arranged by size:
2446 1730 1597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3046 3128 844
Size: 2202 82 3489
Fragments arranged by size:
3489 2202 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 1394
Size: 429 1837 3507
Fragments arranged by size:
3507 1837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2319 2367 4417 4604 1700
Size: 110 509 48 2050 187 2869
Fragments arranged by size:
2869 2050 509 187 110 48
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5773
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 2989 3050 3056 3062 3103 3470 3855 3872
Size: 1017 61 6 6 41 367 385 17
Cuts at: 3872 5324 5410 5422 5768 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1017 385 367 346 86 61
41 17 12 6 6
Bsu36I CC'TnA_GG
Cuts at: 2462 2462
Size: 5773
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 3289 4083 4103 3289
Size: 794 20 4959
Fragments arranged by size:
4959 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3220 4109 5738
Size: 54 1425 50 275 18 1390 889 1629
Cuts at: 5738 8
Size: 43
Fragments arranged by size:
1629 1425 1390 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3703 1353
Size: 2350 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2338 4360 4734 4880 387
Size: 1064 138 749 2022 374 146 1280
Fragments arranged by size:
2022 1280 1064 749 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 2362
Size: 5773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2150 2207 2464 2541 3005 3836
Size: 393 363 155 57 257 77 464 831
Cuts at: 3836 4433 5220 5488 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 831 787 597 464 393 363 268
257 155 77 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2184 2184
Size: 5773
EcoRI G'AATT_C
Cuts at: 2160 3082 3460 2160
Size: 922 378 4473
Fragments arranged by size:
4473 922 378
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 4920 1678
Size: 3242 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2053 2085 2665
Size: 32 135 32 441 32 8 32 580
Cuts at: 2665 2697 2708 2740 4414 4446 4488 4520 1373
Size: 32 11 32 1674 32 42 32 2626
Fragments arranged by size:
2626 1674 580 441 135 42 32 32
32 32 32 32 32 32 11 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3017 748
Size: 2269 3504
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 2075 3372 2075
Size: 1297 4476
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4887 5071 1305
Size: 3582 184 2007
Fragments arranged by size:
3582 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3951
Fragments arranged by size:
3951 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3731 4090 4249 518
Size: 648 2565 359 159 2042
Fragments arranged by size:
2565 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2908 3189 3866 5014 5259 1606
Size: 1302 281 677 1148 245 2120
Fragments arranged by size:
2120 1302 1148 677 281 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 2980 3131 4676 1132
Size: 97 1751 151 1545 2229
Fragments arranged by size:
2229 1751 1545 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2224 1613
Size: 611 5162
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PstI C_TGCA'G
Cuts at: 830 2913 3125 830
Size: 2083 212 3478
Fragments arranged by size:
3478 2083 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2908 2908
Size: 5773
SacI G_AGCT'C
Cuts at: 2364 2364
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 2909 3026 3121 4256 4937 5128 826
Size: 219 1864 117 95 1135 681 191 1471
Fragments arranged by size:
1864 1471 1135 681 219 191 117 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 3100 1872
Size: 1228 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2250 3877 4778 5040 820
Size: 197 926 307 1627 901 262 1553
Fragments arranged by size:
1627 1553 926 901 307 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 2980 3131 1132
Size: 1848 151 3774
Fragments arranged by size:
3774 1848 151
SspI AAT'ATT
Cuts at: 5 52 2835 3697 5
Size: 47 2783 862 2081
Fragments arranged by size:
2783 2081 862 47
StuI AGG'CCT
Cuts at: 1517 2915 1517
Size: 1398 4375
StyI C'CwwG_G
Cuts at: 513 1065 2167 3212 513
Size: 552 1102 1045 3074
Fragments arranged by size:
3074 1102 1045 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3800 3817 3970 4155 4600 325
Size: 1255 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2956 4019 5731
Size: 274 80 33 51 1443 979 1063 1712
Cuts at: 5731 96
Size: 138
Fragments arranged by size:
1712 1443 1063 979 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5733 5765 5733
Size: 32 5741
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 3098 1870
Size: 1228 4545
XmnI GAAnn'nnTTC
Cuts at: 2160 2220 3902 2160
Size: 60 1682 4031
Fragments arranged by size:
4031 1682 60
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI