Restriction map for
pFN21AE9613
(Circular) (Six-base) MAPSORT of: pFN21AE9613.seq Check: 4865 from: 1 to: 5647
pFN21AE9613 5647 bp
With 182 enzymes: SgfI *
March 28, 2012 12:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2619 3457 278
Size: 53 83 186 936 1083 838 2468
Fragments arranged by size:
2468 1083 936 838 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2799 2990 1937
Size: 862 191 4594
Fragments arranged by size:
4594 862 191
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 5094 5362 1632
Size: 2078 1384 268 1917
Fragments arranged by size:
2078 1917 1384 268
AfeI AGC'GCT
Cuts at: 2150 2150
Size: 5647
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 2227 4376 2227
Size: 2149 3498
AleI CACnn'nnGTG
Cuts at: 1166 2748 1166
Size: 1582 4065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 3390 4962 3390
Size: 1572 4075
ApaI G_GGCC'C
Cuts at: 2871 2871
Size: 5647
ApaLI G'TGCA_C
Cuts at: 2741 3704 4860 2741
Size: 963 1156 3528
Fragments arranged by size:
3528 1156 963
ApoI r'AATT_y
Cuts at: 1493 2905 2956 3182 3334 5443 1493
Size: 1412 51 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152 51
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2535 2972 1870
Size: 73 592 437 4545
Fragments arranged by size:
4545 592 437 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2469 2968 4423 618
Size: 325 217 627 682 499 1455 1842
Fragments arranged by size:
1842 1455 682 627 499 325 217
BanII G_rGCy'C
Cuts at: 1391 2484 2871 2966 1391
Size: 1093 387 95 4072
Fragments arranged by size:
4072 1093 387 95
BbeI G_GCGC'C
Cuts at: 2473 2473
Size: 5647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BbvCI CC'TCA_GC
Cuts at: 2586 2586
Size: 5647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2208 3539 4749 2208
Size: 1331 1210 3106
Fragments arranged by size:
3106 1331 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BfrBI ATG'CAT
Cuts at: 2493 2493
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2302 2745 2871 3708 4864 1790
Size: 512 443 126 837 1156 2573
Fragments arranged by size:
2573 1156 837 512 443 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2196 2329 2389
Size: 416 53 83 399 24 779 133 60
Cuts at: 2389 3378 4336 442
Size: 989 958 1753
Fragments arranged by size:
1753 989 958 779 416 399 133 83
60 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2599 4307 1239
Size: 1360 1708 2579
Fragments arranged by size:
2579 1708 1360
Bpu10I CC'TnA_GC
Cuts at: 2586 2586
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2470 2616 3454 3836
Size: 53 83 186 936 937 146 838 382
Cuts at: 3836 4471 275
Size: 635 1451
Fragments arranged by size:
1451 937 936 838 635 382 186 146
83 53
BsaWI w'CCGG_w
Cuts at: 2292 2640 4080 4478 4752 4899 5310 2292
Size: 348 1440 398 274 147 411 2629
Fragments arranged by size:
2629 1440 411 398 348 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5422
Fragments arranged by size:
5422 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2106 2344 2645 1186
Size: 920 238 301 4188
Fragments arranged by size:
4188 920 301 238
BseYI C'CCAG_C
Cuts at: 2063 4850 5346 2063
Size: 2787 496 2364
Fragments arranged by size:
2787 2364 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2358 1163
Size: 573 622 4452
Fragments arranged by size:
4452 622 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2879 3097 3858 4886 1540
Size: 275 186 878 218 761 1028 2301
Fragments arranged by size:
2301 1028 878 761 275 218 186
BsiHKAI G_wGCw'C
Cuts at: 2078 2393 2484 2745 3708 3793 4864 2078
Size: 315 91 261 963 85 1071 2861
Fragments arranged by size:
2861 1071 963 315 261 91 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2302 2393 2484 2745 2871 2966
Size: 399 288 224 91 91 261 126 95
Cuts at: 2966 3708 3793 4864 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 288 261 224 126
95 91 91 85
BspEI T'CCGG_A
Cuts at: 2292 2640 5310 2292
Size: 348 2670 2629
Fragments arranged by size:
2670 2629 348
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 2785 3002 844
Size: 1825 116 217 3489
Fragments arranged by size:
3489 1825 217 116
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 2425 1184
Size: 1241 4406
BstYI r'GATC_y
Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642
Size: 1005 367 385 17 1452 86 12 346
Cuts at: 5642 1972
Size: 1977
Fragments arranged by size:
1977 1452 1005 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2800 2800
Size: 5647
BtgI C'CryG_G
Cuts at: 513 1065 2485 2593 513
Size: 552 1420 108 3567
Fragments arranged by size:
3567 1420 552 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2823 3163 3957 3977 2823
Size: 340 794 20 4493
Fragments arranged by size:
4493 794 340 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2032 4654 809
Size: 507 716 2622 1802
Fragments arranged by size:
2622 1802 716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3094 3983 5612
Size: 54 1425 50 275 18 1264 889 1629
Cuts at: 5612 8
Size: 43
Fragments arranged by size:
1629 1425 1264 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3577 1353
Size: 2224 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2297 4234 4608 4754 387
Size: 1064 138 708 1937 374 146 1280
Fragments arranged by size:
1937 1280 1064 708 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 2482
Size: 5647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2599 3710 4307 5094 5362 1239
Size: 393 967 1111 597 787 268 1524
Fragments arranged by size:
1524 1111 967 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2503 2653 2503
Size: 150 5497
EcoRI G'AATT_C
Cuts at: 2905 2956 3334 2905
Size: 51 378 5218
Fragments arranged by size:
5218 378 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2261 2032
Size: 229 5418
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2152 2473 4794 1678
Size: 474 321 2321 2531
Fragments arranged by size:
2531 2321 474 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 4288 4320 4362
Size: 32 135 32 641 32 2043 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 2043 641 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2025 2991 3237 669
Size: 988 281 87 966 246 3079
Fragments arranged by size:
3079 988 966 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KasI G'GCGC_C
Cuts at: 2469 2469
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2030 2859 4761 4945 1305
Size: 725 829 1902 184 2007
Fragments arranged by size:
2007 1902 829 725 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2748 3605 3964 4123 518
Size: 648 843 739 857 359 159 2042
Fragments arranged by size:
2042 857 843 739 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2314 3063 3740 4888 5133 1606
Size: 708 749 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 708 677 245
NarI GG'CG_CC
Cuts at: 2470 2470
Size: 5647
NcoI C'CATG_G
Cuts at: 513 1065 2593 513
Size: 552 1528 3567
Fragments arranged by size:
3567 1528 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NsiI A_TGCA'T
Cuts at: 2495 2495
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 2599 1108
Size: 1329 162 4156
Fragments arranged by size:
4156 1329 162
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 2653 2503
Size: 150 5497
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspOMI G'GGCC_C
Cuts at: 2867 2867
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SacI G_AGCT'C
Cuts at: 2484 2484
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2224 2995 4130 4811 5002 826
Size: 219 1179 771 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 771 681 219 191
SfoI GGC'GCC
Cuts at: 2471 2471
Size: 5647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2537 2974 1872
Size: 665 437 4545
Fragments arranged by size:
4545 665 437
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2454 2593 3086 513
Size: 552 1389 139 493 3074
Fragments arranged by size:
3074 1389 552 493 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 3691 3844 4029 4474 325
Size: 1255 2094 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2415 3893 5605
Size: 274 80 33 51 1443 438 1478 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1478 1443 438 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 2501 1316
Size: 1185 4462
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2535 2972 1870
Size: 665 437 4545
Fragments arranged by size:
4545 665 437
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2617 3455 276
Size: 53 83 186 936 1083 838 2468
Fragments arranged by size:
2468 1083 936 838 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BplI BsiWI BsmBI
BstXI Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI