Restriction map for
pFN21AE9611
(Circular) (Six-base) MAPSORT of: pFN21AE9611.seq Check: 6528 from: 1 to: 5605
pFN21AE9611 5605 bp
With 182 enzymes: SgfI *
March 28, 2012 12:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2754 3415 278
Size: 53 83 186 936 1218 661 2468
Fragments arranged by size:
2468 1218 936 661 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2130 2579 3668 5052 5320 1632
Size: 498 449 1089 1384 268 1917
Fragments arranged by size:
1917 1384 1089 498 449 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 2762 4504 5481 1203
Size: 1559 1742 977 1327
Fragments arranged by size:
1742 1559 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 2544 3348 4920 2544
Size: 804 1572 3229
Fragments arranged by size:
3229 1572 804
ApaI G_GGCC'C
Cuts at: 2628 2628
Size: 5605
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2914 3140 3292 5401 1493
Size: 1421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 1870
Size: 73 987 4545
Fragments arranged by size:
4545 987 73
BamHI G'GATC_C
Cuts at: 2677 2935 3302 2677
Size: 258 367 4980
Fragments arranged by size:
4980 367 258
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2205 2926 4381 618
Size: 325 217 627 418 721 1455 1842
Fragments arranged by size:
1842 1455 721 627 418 325 217
BanII G_rGCy'C
Cuts at: 1391 2628 2924 1391
Size: 1237 296 4072
Fragments arranged by size:
4072 1237 296
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2467 928
Size: 971 568 4066
Fragments arranged by size:
4066 971 568
BbvCI CC'TCA_GC
Cuts at: 2189 2189
Size: 5605
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2824 2858 3796 3830 1903
Size: 34 887 34 938 34 3678
Fragments arranged by size:
3678 938 887 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3497 4707 2445
Size: 1052 1210 3343
Fragments arranged by size:
3343 1210 1052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2628 3666 4822 1790
Size: 838 1038 1156 2573
Fragments arranged by size:
2573 1156 1038 838
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2465 2631 3336
Size: 416 53 83 399 24 1048 166 705
Cuts at: 3336 4294 442
Size: 958 1753
Fragments arranged by size:
1753 1048 958 705 416 399 166 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4265 1239
Size: 3026 2579
Bpu10I CC'TnA_GC
Cuts at: 2189 2189
Size: 5605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2072 2905 4268 882
Size: 617 573 833 1363 2219
Fragments arranged by size:
2219 1363 833 617 573
BsaAI yAC'GTr
Cuts at: 493 1204 3396 493
Size: 711 2192 2702
Fragments arranged by size:
2702 2192 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2173 2751 3412 3794
Size: 53 83 186 936 640 578 661 382
Cuts at: 3794 4429 275
Size: 635 1451
Fragments arranged by size:
1451 936 661 640 635 578 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2448 4038 4436 4710 4857 5268 2448
Size: 1590 398 274 147 411 2785
Fragments arranged by size:
2785 1590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2358 2388 2763 2793 1178
Size: 30 165 30 955 30 375 30 3990
Fragments arranged by size:
3990 955 375 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2379 2813 1186
Size: 1193 434 3978
Fragments arranged by size:
3978 1193 434
BseYI C'CCAG_C
Cuts at: 4808 5304 4808
Size: 496 5109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 1163
Size: 573 411 4621
Fragments arranged by size:
4621 573 411
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2146 3666 3751 4822 2146
Size: 1520 85 1071 2929
Fragments arranged by size:
2929 1520 1071 85
BsmI GAATG_Cn'
Cuts at: 2563 3116 3209 2563
Size: 553 93 4959
Fragments arranged by size:
4959 553 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2628 2924 3666 3751 4822 1391
Size: 399 356 482 296 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 356 296 85
BspEI T'CCGG_A
Cuts at: 2448 5268 2448
Size: 2820 2785
BspHI T'CATG_A
Cuts at: 2815 3494 5224 2815
Size: 679 1730 3196
Fragments arranged by size:
3196 1730 679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 844
Size: 2116 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2208 2589 4249 4436 1700
Size: 110 398 381 1660 187 2869
Fragments arranged by size:
2869 1660 398 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2125 2234 794
Size: 1331 109 4165
Fragments arranged by size:
4165 1331 109
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2093 2677 2935 3302 3687 3704 5156 5242
Size: 121 584 258 367 385 17 1452 86
Cuts at: 5242 5254 5600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 584 385 367 346 258 121
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 3121 3915 3935 3121
Size: 794 20 4791
Fragments arranged by size:
4791 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3052 3941 5570
Size: 54 1425 50 275 18 1222 889 1629
Cuts at: 5570 8
Size: 43
Fragments arranged by size:
1629 1425 1222 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 2283 2484 2745 3535 1353
Size: 753 177 201 261 790 3423
Fragments arranged by size:
3423 790 753 261 201 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2579 3668 4265 5052 5320 1239
Size: 393 498 449 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 597 498 449 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2625 2725 2625
Size: 100 5505
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2162 2162
Size: 5605
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2358 2390 4246
Size: 32 135 32 452 32 302 32 1856
Cuts at: 4246 4278 4320 4352 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1856 452 302 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2865 4719 4903 1305
Size: 1560 1854 184 2007
Fragments arranged by size:
2007 1854 1560 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3783
Fragments arranged by size:
3783 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3563 3922 4081 518
Size: 648 2397 359 159 2042
Fragments arranged by size:
2397 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3021 3698 4846 5091 1606
Size: 1415 677 1148 245 2120
Fragments arranged by size:
2120 1415 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2232 2766 2963 4508 1132
Size: 97 1003 534 197 1545 2229
Fragments arranged by size:
2229 1545 1003 534 197 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 2762 4504 2762
Size: 1742 3863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2260 2793 1108
Size: 1152 533 3920
Fragments arranged by size:
3920 1152 533
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2725
Size: 5605
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PspOMI G'GGCC_C
Cuts at: 2624 2624
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2121 2322 2957 830
Size: 1291 201 635 3478
Fragments arranged by size:
3478 1291 635 201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SapI GCTCTTCn'nnn_
Cuts at: 2106 2106
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 2386 3853 5565 2386
Size: 1467 1712 2426
Fragments arranged by size:
2426 1712 1467
SfcI C'TryA_G
Cuts at: 826 1045 2117 2318 2953 4088 4769 4960 826
Size: 219 1072 201 635 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 681 635 219 201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 2208 1700
Size: 508 5097
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 2507 3529 5
Size: 47 2455 1022 2081
Fragments arranged by size:
2455 2081 1022 47
StuI AGG'CCT
Cuts at: 1517 2340 1517
Size: 823 4782
StyI C'CwwG_G
Cuts at: 513 1065 2004 3044 513
Size: 552 939 1040 3074
Fragments arranged by size:
3074 1040 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3632 3649 3802 3987 4432 325
Size: 1255 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2384 3851 5563
Size: 274 80 33 51 1443 407 1467 1712
Cuts at: 5563 96
Size: 138
Fragments arranged by size:
1712 1467 1443 407 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 2429 1316
Size: 1113 4492
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2257
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2752 3413 276
Size: 53 83 186 936 1218 661 2468
Fragments arranged by size:
2468 1218 936 661 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI