Restriction map for
pFN21AE9609
(Circular) (Six-base) MAPSORT of: pFN21AE9609.seq Check: 5971 from: 1 to: 5536
pFN21AE9609 5536 bp
With 182 enzymes: SgfI *
March 28, 2012 12:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 2806 2879 1937
Size: 717 152 73 4594
Fragments arranged by size:
4594 717 152 73
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2268 2321 2609 3599 4983 5251 1632
Size: 527 109 53 288 990 1384 268 1917
Fragments arranged by size:
1917 1384 990 527 288 268 109 53
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 4435 5412 1203
Size: 3232 977 1327
Fragments arranged by size:
3232 1327 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5536
AlwNI CAG_nnn'CTG
Cuts at: 2503 2650 3279 4851 2503
Size: 147 629 1572 3188
Fragments arranged by size:
3188 1572 629 147
ApaLI G'TGCA_C
Cuts at: 3593 4749 3593
Size: 1156 4380
ApoI r'AATT_y
Cuts at: 1493 2827 2845 3071 3223 5332 1493
Size: 1334 18 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2861 1870
Size: 73 918 4545
Fragments arranged by size:
4545 918 73
BamHI G'GATC_C
Cuts at: 2866 3233 2866
Size: 367 5169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2857 4312 618
Size: 325 217 627 1070 1455 1842
Fragments arranged by size:
1842 1455 1070 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2855 1391
Size: 1464 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2256 928
Size: 971 357 4208
Fragments arranged by size:
4208 971 357
BbvCI CC'TCA_GC
Cuts at: 2055 2688 2694 2055
Size: 633 6 4897
Fragments arranged by size:
4897 633 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2717 2751 3727 3761 1903
Size: 34 780 34 976 34 3678
Fragments arranged by size:
3678 976 780 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4147 136
Size: 107 122 71 631 605 337 2138 1525
Fragments arranged by size:
2138 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 3597 4753 1790
Size: 1807 1156 2573
Fragments arranged by size:
2573 1807 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 2627 2838 4196 1239
Size: 1163 225 211 1358 2579
Fragments arranged by size:
2579 1358 1163 225 211
Bpu10I CC'TnA_GC
Cuts at: 2055 2208 2223 2280 2688 2694 2055
Size: 153 15 57 408 6 4897
Fragments arranged by size:
4897 408 153 57 15 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3661 4526 4824 3661
Size: 865 298 4373
Fragments arranged by size:
4373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2234 2769 4199 882
Size: 617 735 535 1430 2219
Fragments arranged by size:
2219 1430 735 617 535
BsaAI yAC'GTr
Cuts at: 493 1204 2323 3327 493
Size: 711 1119 1004 2702
Fragments arranged by size:
2702 1119 1004 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3343 3725 4360 275
Size: 53 83 186 936 1810 382 635 1451
Fragments arranged by size:
1810 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3969 4367 4641 4788 5199 3969
Size: 398 274 147 411 4306
Fragments arranged by size:
4306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5311
Fragments arranged by size:
5311 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 1186
Size: 922 4614
BseYI C'CCAG_C
Cuts at: 2724 4739 5235 2724
Size: 2015 496 3025
Fragments arranged by size:
3025 2015 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4963
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2986 3747 4775 1540
Size: 275 186 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 3597 3682 4753 2322
Size: 1275 85 1071 3105
Fragments arranged by size:
3105 1275 1071 85
BsmI GAATG_Cn'
Cuts at: 3047 3140 3047
Size: 93 5443
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2855 3597 3682 4753 1391
Size: 399 532 533 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 533 532 399 85
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 3425 5155 3425
Size: 1730 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2821 2891 844
Size: 1977 70 3489
Fragments arranged by size:
3489 1977 70
BsrBI CCG'CTC
Cuts at: 1394 1823 2611 3423 1394
Size: 429 788 812 3507
Fragments arranged by size:
3507 812 788 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4031 4205 65
Size: 1183 2783 174 1396
Fragments arranged by size:
2783 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4180 4367 1700
Size: 110 2370 187 2869
Fragments arranged by size:
2869 2370 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 2254 3596 4608 2254
Size: 1342 1012 3182
Fragments arranged by size:
3182 1342 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5536
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5536
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 2119
Size: 5536
BstYI r'GATC_y
Cuts at: 1972 2115 2866 3233 3618 3635 5087 5173 5185
Size: 143 751 367 385 17 1452 86 12
Cuts at: 5185 5531 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 751 385 367 346 143 86
17 12
BstZ17I GTA'TAC
Cuts at: 2655 2655
Size: 5536
Bsu36I CC'TnA_GG
Cuts at: 2682 2682
Size: 5536
BtgI C'CryG_G
Cuts at: 513 1065 2004 2439 2766 513
Size: 552 939 435 327 3283
Fragments arranged by size:
3283 939 552 435 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 3052 3846 3866 2217
Size: 835 794 20 3887
Fragments arranged by size:
3887 835 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2436 2983 3872
Size: 54 1425 50 275 18 606 547 889
Cuts at: 3872 5501 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 606 547 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2983 1537
Size: 275 1171 4090
Fragments arranged by size:
4090 1171 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3466 1353
Size: 2113 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 2522 4123 4497 4643 387
Size: 1064 138 453 480 1601 374 146 1280
Fragments arranged by size:
1601 1280 1064 480 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2268 2321 2402 2609 2627 2838
Size: 393 527 109 53 81 207 18 211
Cuts at: 2838 3599 4196 4983 5251 1239
Size: 761 597 787 268 1524
Fragments arranged by size:
1524 787 761 597 527 393 268 211
207 109 81 53 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 2090 2207 2348 2054
Size: 36 117 141 5242
Fragments arranged by size:
5242 141 117 36
EcoO109I rG'GnC_Cy
Cuts at: 2047 2353 2047
Size: 306 5230
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 4683 1678
Size: 3005 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2530 2562 4177 4209 4251
Size: 32 135 32 958 32 1615 32 42
Cuts at: 4251 4283 1373
Size: 32 2626
Fragments arranged by size:
2626 1615 958 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2880 3126 669
Size: 988 281 942 246 3079
Fragments arranged by size:
3079 988 942 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 2271 3135 2271
Size: 864 4672
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4650 4834 1305
Size: 3345 184 2007
Fragments arranged by size:
3345 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2438 10
Size: 54 1425 343 606 3108
Fragments arranged by size:
3108 1425 606 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3494 3853 4012 518
Size: 648 2328 359 159 2042
Fragments arranged by size:
2328 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2952 3629 4777 5022 1606
Size: 409 937 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 677 409 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2439 2766 513
Size: 552 939 435 327 3283
Fragments arranged by size:
3283 939 552 435 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2894 4439 1132
Size: 97 1665 1545 2229
Fragments arranged by size:
2229 1665 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5536
PciI A'CATG_T
Cuts at: 4435 4435
Size: 5536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5536
PfoI T'CCnGG_A
Cuts at: 2397 2397
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2047
Size: 5536
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PstI C_TGCA'G
Cuts at: 830 2038 2091 2168 2196 2499 2521 2625 2664
Size: 1208 53 77 28 303 22 104 39
Cuts at: 2664 2888 830
Size: 224 3478
Fragments arranged by size:
3478 1208 303 224 104 77 53 39
28 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SbfI CC_TGCA'GG
Cuts at: 2091 2664 2888 2091
Size: 573 224 4739
Fragments arranged by size:
4739 573 224
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2034 2079 2087 2164 2192 2311 2495
Size: 219 989 45 8 77 28 119 184
Cuts at: 2495 2517 2621 2660 2884 4019 4700 4891 826
Size: 22 104 39 224 1135 681 191 1471
Fragments arranged by size:
1471 1135 989 681 224 219 191 184
119 104 77 45 39 28 22 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2863 1872
Size: 991 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3640 4541 4803 820
Size: 197 926 1697 901 262 1553
Fragments arranged by size:
1697 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 2085 1517
Size: 568 4968
StyI C'CwwG_G
Cuts at: 513 1065 2004 2238 2349 2439 2766 2975 513
Size: 552 939 234 111 90 327 209 3074
Fragments arranged by size:
3074 939 552 327 234 209 111 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3563 3580 3733 3918 4363 325
Size: 1255 1983 17 153 185 445 1498
Fragments arranged by size:
1983 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2753 3782 5494
Size: 274 80 33 51 1443 776 1029 1712
Cuts at: 5494 96
Size: 138
Fragments arranged by size:
1712 1443 1029 776 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5496 5528 5496
Size: 32 5504
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2431 2431
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2861 1870
Size: 991 4545
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI