Restriction map for
pFN21AE9606
(Circular) (Six-base) MAPSORT of: pFN21AE9606.seq Check: 4336 from: 1 to: 5404
pFN21AE9606 5404 bp
With 182 enzymes: SgfI *
March 28, 2012 12:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 1937
Size: 810 4594
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3467 4851 5119 1632
Size: 1835 1384 268 1917
Fragments arranged by size:
1917 1835 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 2178 2454 4303 5280 1203
Size: 975 276 1849 977 1327
Fragments arranged by size:
1849 1327 977 975 276
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 2026 1166
Size: 860 4544
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5404
AlwNI CAG_nnn'CTG
Cuts at: 3147 4719 3147
Size: 1572 3832
ApaLI G'TGCA_C
Cuts at: 3461 4617 3461
Size: 1156 4248
ApoI r'AATT_y
Cuts at: 1493 2713 2939 3091 5200 1493
Size: 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2729 1870
Size: 73 176 610 4545
Fragments arranged by size:
4545 610 176 73
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2725 4180 618
Size: 325 217 627 938 1455 1842
Fragments arranged by size:
1842 1455 938 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2165 2723 1391
Size: 774 558 4072
Fragments arranged by size:
4072 774 558
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 2181 928
Size: 971 222 60 4151
Fragments arranged by size:
4151 971 222 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3595 3629 1903
Size: 34 1658 34 3678
Fragments arranged by size:
3678 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4015 136
Size: 107 122 71 631 605 2343 1525
Fragments arranged by size:
2343 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5399 5399
Size: 5404
BlpI GC'TnA_GC
Cuts at: 2291 2821 2291
Size: 530 4874
Bme1580I G_kGCm'C
Cuts at: 1790 3465 4621 1790
Size: 1675 1156 2573
Fragments arranged by size:
2573 1675 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4093 442
Size: 416 53 83 399 24 1718 958 1753
Fragments arranged by size:
1753 1718 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 4064 1239
Size: 992 1833 2579
Fragments arranged by size:
2579 1833 992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 3529 4394 4692 2432
Size: 1097 865 298 3144
Fragments arranged by size:
3144 1097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4067 882
Size: 617 2568 2219
Fragments arranged by size:
2568 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2455 3195 493
Size: 711 1251 740 2702
Fragments arranged by size:
2702 1251 740 711
BsaBI GATnn'nnATC
Cuts at: 5398 5398
Size: 5404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3211 3593 4228 275
Size: 53 83 186 936 1678 382 635 1451
Fragments arranged by size:
1678 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3837 4235 4509 4656 5067 3837
Size: 398 274 147 411 4174
Fragments arranged by size:
4174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5179
Fragments arranged by size:
5179 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2077 2228 2231 2249 1186
Size: 891 151 3 18 4341
Fragments arranged by size:
4341 891 151 18 3
BseYI C'CCAG_C
Cuts at: 2278 2295 4607 5103 2278
Size: 17 2312 496 2579
Fragments arranged by size:
2579 2312 496 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4831
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2276 2854 3615 4643 1540
Size: 275 186 275 578 761 1028 2301
Fragments arranged by size:
2301 1028 761 578 275 275 186
BsiHKAI G_wGCw'C
Cuts at: 2165 2261 2438 3465 3550 4621 2165
Size: 96 177 1027 85 1071 2948
Fragments arranged by size:
2948 1071 1027 177 96 85
BsmI GAATG_Cn'
Cuts at: 2915 3008 2915
Size: 93 5311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2261 2438 2723 3465 3550 4621
Size: 399 375 96 177 285 742 85 1071
Cuts at: 4621 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 375 285 177 96
85
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 3293 5023 3293
Size: 1730 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2759 844
Size: 1915 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 1394
Size: 429 1468 3507
Fragments arranged by size:
3507 1468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3899 4073 65
Size: 1183 2651 174 1396
Fragments arranged by size:
2651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2268 4048 4235 1700
Size: 110 458 1780 187 2869
Fragments arranged by size:
2869 1780 458 187 110
BsrGI T'GTAC_A
Cuts at: 96 2457 96
Size: 2361 3043
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 3464 4476 3464
Size: 1012 4392
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2040 794
Size: 1246 4158
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2734 3101 3486 3503 4955 5041 5053 5399
Size: 762 367 385 17 1452 86 12 346
Cuts at: 5399 1972
Size: 1977
Fragments arranged by size:
1977 1452 762 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4665
Fragments arranged by size:
4665 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 3714 3734 2920
Size: 794 20 4590
Fragments arranged by size:
4590 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2273 2301
Size: 54 1425 50 275 18 357 86 28
Cuts at: 2301 2851 3740 5369 8
Size: 550 889 1629 43
Fragments arranged by size:
1629 1425 889 550 357 275 86 54
50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 2273 2851 1537
Size: 275 461 578 4090
Fragments arranged by size:
4090 578 461 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3334 1353
Size: 1981 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3991 4365 4511 387
Size: 1064 138 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2163 2163
Size: 5404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 3467 4064 4851 5119 1239
Size: 393 599 1236 597 787 268 1524
Fragments arranged by size:
1524 1236 787 599 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2541 2541
Size: 5404
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 5404
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 4551 1678
Size: 2873 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2657 2689 4045 4077 4119
Size: 32 135 32 1085 32 1356 32 42
Cuts at: 4119 4151 1373
Size: 32 2626
Fragments arranged by size:
2626 1356 1085 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 2994 669
Size: 988 281 810 246 3079
Fragments arranged by size:
3079 988 810 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 3003 3003
Size: 5404
MluI A'CGCG_T
Cuts at: 2178 5280 2178
Size: 3102 2302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 10
Size: 54 1425 343 471 3111
Fragments arranged by size:
3111 1425 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2026 3362 3721 3880 518
Size: 648 860 1336 359 159 2042
Fragments arranged by size:
2042 1336 860 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2209 2245 2278 2820 3497 4645 4890
Size: 528 75 36 33 542 677 1148 245
Cuts at: 4890 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 542 528 245 75 36
33
NaeI GCC'GGC
Cuts at: 2270 2270
Size: 5404
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5404
NgoMIV G'CCGG_C
Cuts at: 2268 2268
Size: 5404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2273 2851 2273
Size: 578 4826
NspI r_CATG'y
Cuts at: 1132 1229 2158 2762 4307 1132
Size: 97 929 604 1545 2229
Fragments arranged by size:
2229 1545 929 604 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5404
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5404
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2541 2541
Size: 5404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5404
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PspXI vC'TCGA_Gb
Cuts at: 2119 2119
Size: 5404
PstI C_TGCA'G
Cuts at: 830 2756 830
Size: 1926 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
PvuII CAG'CTG
Cuts at: 2134 2209 2134
Size: 75 5329
SacI G_AGCT'C
Cuts at: 2165 2165
Size: 5404
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2752 3887 4568 4759 826
Size: 219 1707 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2731 1872
Size: 859 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2119 2411 3508 4409 4671 820
Size: 197 926 176 292 1097 901 262 1553
Fragments arranged by size:
1553 1097 926 901 292 262 197 176
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5404
StyI C'CwwG_G
Cuts at: 513 1065 2004 2843 513
Size: 552 939 839 3074
Fragments arranged by size:
3074 939 839 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2007 2287 3431 3448 3601 3786 4231
Size: 1255 427 280 1144 17 153 185 445
Cuts at: 4231 325
Size: 1498
Fragments arranged by size:
1498 1255 1144 445 427 280 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2457 3650 5362
Size: 274 80 33 51 1443 480 1193 1712
Cuts at: 5362 96
Size: 138
Fragments arranged by size:
1712 1443 1193 480 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5364 5396 5364
Size: 32 5372
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 2119 1943
Size: 176 5228
XmaI C'CCGG_G
Cuts at: 1870 2729 1870
Size: 859 4545
XmnI GAAnn'nnTTC
Cuts at: 3533 3533
Size: 5404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI