Restriction map for
pFN21AE9602
(Circular) (Six-base) MAPSORT of: pFN21AE9602.seq Check: 392 from: 1 to: 5296
pFN21AE9602 5296 bp
With 182 enzymes: SgfI *
March 28, 2012 12:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2101 2209 2101
Size: 108 5188
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3106 278
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2379 2639 1937
Size: 442 260 4594
Fragments arranged by size:
4594 442 260
Acc65I G'GTAC_C
Cuts at: 2617 2617
Size: 5296
AclI AA'CG_TT
Cuts at: 1563 3423 3796 1563
Size: 1860 373 3063
Fragments arranged by size:
3063 1860 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 2294 2315 3359 4743 5011 1632
Size: 617 45 21 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1044 617 268 45 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5099
AflIII A'CryG_T
Cuts at: 1203 4195 5172 1203
Size: 2992 977 1327
Fragments arranged by size:
2992 1327 977
AgeI A'CCGG_T
Cuts at: 4127 4127
Size: 5296
AhdI GACnn_n'nnGTC
Cuts at: 4025 4025
Size: 5296
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2160 1184
Size: 976 4320
AlwNI CAG_nnn'CTG
Cuts at: 2044 2251 3039 4611 2044
Size: 207 788 1572 2729
Fragments arranged by size:
2729 1572 788 207
ApaLI G'TGCA_C
Cuts at: 2222 3353 4509 2222
Size: 1131 1156 3009
Fragments arranged by size:
3009 1156 1131
ApoI r'AATT_y
Cuts at: 1493 2552 2605 2831 2983 5092 1493
Size: 1059 53 226 152 2109 1697
Fragments arranged by size:
2109 1697 1059 226 152 53
AseI AT'TA_AT
Cuts at: 160 1032 3850 160
Size: 872 2818 1606
Fragments arranged by size:
2818 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
AvaI C'yCGr_G
Cuts at: 1870 1943 2621 1870
Size: 73 678 4545
Fragments arranged by size:
4545 678 73
BamHI G'GATC_C
Cuts at: 2626 2993 2626
Size: 367 4929
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2100 2144 2617 4072
Size: 325 217 627 273 40 44 473 1455
Cuts at: 4072 618
Size: 1842
Fragments arranged by size:
1842 1455 627 473 325 273 217 44
40
BanII G_rGCy'C
Cuts at: 1391 2337 2615 1391
Size: 946 278 4072
Fragments arranged by size:
4072 946 278
BbeI G_GCGC'C
Cuts at: 2064 2104 2064
Size: 40 5256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3487 3521 1903
Size: 34 1550 34 3678
Fragments arranged by size:
3678 1550 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3188 4398 3188
Size: 1210 4086
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 3907 136
Size: 107 122 71 631 605 386 1849 1525
Fragments arranged by size:
1849 1525 631 605 386 122 107 71
BglII A'GATC_T
Cuts at: 5291 5291
Size: 5296
BlpI GC'TnA_GC
Cuts at: 2048 2713 2048
Size: 665 4631
Bme1580I G_kGCm'C
Cuts at: 1790 2226 3357 4513 1790
Size: 436 1131 1156 2573
Fragments arranged by size:
2573 1156 1131 436
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5296
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2499 3027 3985
Size: 416 53 83 399 24 1082 528 958
Cuts at: 3985 442
Size: 1753
Fragments arranged by size:
1753 1082 958 528 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5296
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2186 2186
Size: 5296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2530 2593 3956 1239
Size: 1139 152 63 1363 2579
Fragments arranged by size:
2579 1363 1139 152 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 3421 4286 4584 2321
Size: 1100 865 298 3033
Fragments arranged by size:
3033 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3959 882
Size: 617 2460 2219
Fragments arranged by size:
2460 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2419 3087 493
Size: 711 1215 668 2702
Fragments arranged by size:
2702 1215 711 668
BsaBI GATnn'nnATC
Cuts at: 5290 5290
Size: 5296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2101 3103 3485
Size: 53 83 186 936 528 40 1002 382
Cuts at: 3485 4120 275
Size: 635 1451
Fragments arranged by size:
1451 1002 936 635 528 382 186 83
53 40
BsaWI w'CCGG_w
Cuts at: 3729 4127 4401 4548 4959 3729
Size: 398 274 147 411 4066
Fragments arranged by size:
4066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5071
Fragments arranged by size:
5071 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2294 1186
Size: 856 252 4188
Fragments arranged by size:
4188 856 252
BseYI C'CCAG_C
Cuts at: 2056 2095 2258 2273 2514 2538 4499 4995 2056
Size: 39 163 15 241 24 1961 496 2357
Fragments arranged by size:
2357 1961 496 241 163 39 24 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4723
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2412 2746 3507 4535 1540
Size: 275 186 411 334 761 1028 2301
Fragments arranged by size:
2301 1028 761 411 334 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2337 3357 3442 4513 2226
Size: 111 1020 85 1071 3009
Fragments arranged by size:
3009 1071 1020 111 85
BsmI GAATG_Cn'
Cuts at: 2807 2900 2807
Size: 93 5203
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2337 2615 3357 3442 4513 1391
Size: 399 436 111 278 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 399 278 111 85
BspEI T'CCGG_A
Cuts at: 4959 4959
Size: 5296
BspHI T'CATG_A
Cuts at: 3185 4915 3185
Size: 1730 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2101 2209 2651 844
Size: 1257 108 442 3489
Fragments arranged by size:
3489 1257 442 108
BsrBI CCG'CTC
Cuts at: 1394 1823 2122 3183 1394
Size: 429 299 1061 3507
Fragments arranged by size:
3507 1061 429 299
BsrDI GCAATG_nn'
Cuts at: 65 1248 3791 3965 65
Size: 1183 2543 174 1396
Fragments arranged by size:
2543 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3940 4127 1700
Size: 110 2130 187 2869
Fragments arranged by size:
2869 2130 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5296
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5296
BssSI C'ACGA_G
Cuts at: 2105 3356 4368 2105
Size: 1251 1012 3033
Fragments arranged by size:
3033 1251 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5296
BstBI TT'CG_AA
Cuts at: 4100 4100
Size: 5296
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5296
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2527 2326
Size: 201 5095
BstYI r'GATC_y
Cuts at: 1972 2166 2353 2626 2993 3378 3395 4847 4933
Size: 194 187 273 367 385 17 1452 86
Cuts at: 4933 4945 5291 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 273 194 187
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2175 513
Size: 552 939 171 3634
Fragments arranged by size:
3634 939 552 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4557
Fragments arranged by size:
4557 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 3606 3626 2812
Size: 794 20 4482
Fragments arranged by size:
4482 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5261
DraI TTT'AAA
Cuts at: 2600 2600
Size: 5296
DraIII CAC_nnn'GTG
Cuts at: 2592 2592
Size: 5296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2393 4303 809
Size: 507 1077 1910 1802
Fragments arranged by size:
1910 1802 1077 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2409 2743
Size: 54 1425 50 275 18 177 402 334
Cuts at: 2743 3632 5261 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 402 334 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2409 2743 1537
Size: 275 597 334 4090
Fragments arranged by size:
4090 597 334 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 3226 1353
Size: 972 901 3423
Fragments arranged by size:
3423 972 901
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2366 3883 4257 4403 387
Size: 1064 138 777 1517 374 146 1280
Fragments arranged by size:
1517 1280 1064 777 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 2335
Size: 5296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2294 2315 2378 2530 2593 3359
Size: 393 617 45 21 63 152 63 766
Cuts at: 3359 3956 4743 5011 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 766 617 597 393 268 152
63 63 45 21
EcoRI G'AATT_C
Cuts at: 2605 2983 2605
Size: 378 4918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2279 2279
Size: 5296
FspI TGC'GCA
Cuts at: 3802 3802
Size: 5296
HaeII r_GCGC'y
Cuts at: 1678 2064 2104 4443 1678
Size: 386 40 2339 2531
Fragments arranged by size:
2531 2339 386 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3937 3969 4011 4043 1373
Size: 32 135 32 2365 32 42 32 2626
Fragments arranged by size:
2626 2365 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2380 2640 2886 669
Size: 988 281 442 260 246 3079
Fragments arranged by size:
3079 988 442 281 260 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5296
HpaI GTT'AAC
Cuts at: 2886 2886
Size: 5296
KasI G'GCGC_C
Cuts at: 2060 2100 2060
Size: 40 5256
KpnI G_GTAC'C
Cuts at: 2621 2621
Size: 5296
MfeI C'AATT_G
Cuts at: 2895 2895
Size: 5296
MluI A'CGCG_T
Cuts at: 5172 5172
Size: 5296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4410 4594 1305
Size: 3105 184 2007
Fragments arranged by size:
3105 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3474
Fragments arranged by size:
3474 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3254 3613 3772 518
Size: 648 2088 359 159 2042
Fragments arranged by size:
2088 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2251 2712 3389 4537 4782 1606
Size: 552 93 461 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 461 245 93
NarI GG'CG_CC
Cuts at: 2061 2101 2061
Size: 40 5256
NcoI C'CATG_G
Cuts at: 513 1065 2004 2175 513
Size: 552 939 171 3634
Fragments arranged by size:
3634 939 552 171
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5296
NotI GC'GGCC_GC
Cuts at: 2743 2743
Size: 5296
NspI r_CATG'y
Cuts at: 1132 1229 2654 4199 1132
Size: 97 1425 1545 2229
Fragments arranged by size:
2229 1545 1425 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5296
PciI A'CATG_T
Cuts at: 4195 4195
Size: 5296
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5296
PfoI T'CCnGG_A
Cuts at: 2546 2546
Size: 5296
PmeI GTTT'AAAC
Cuts at: 2600 2600
Size: 5296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 4878 4910 3365
Size: 32 1481 32 3751
Fragments arranged by size:
3751 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2289 1613
Size: 676 4620
PsiI TTA'TAA
Cuts at: 2866 2866
Size: 5296
PstI C_TGCA'G
Cuts at: 830 2247 2648 830
Size: 1417 401 3478
Fragments arranged by size:
3478 1417 401
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5296
PvuII CAG'CTG
Cuts at: 2158 2251 2158
Size: 93 5203
SacI G_AGCT'C
Cuts at: 2337 2337
Size: 5296
SalI G'TCGA_C
Cuts at: 1936 2378 2638 1936
Size: 442 260 4594
Fragments arranged by size:
4594 442 260
SapI GCTCTTCn'nnn_
Cuts at: 2325 2325
Size: 5296
SbfI CC_TGCA'GG
Cuts at: 2648 2648
Size: 5296
ScaI AGT'ACT
Cuts at: 3544 5256 3544
Size: 1712 3584
SfcI C'TryA_G
Cuts at: 826 1045 2243 2644 3779 4460 4651 826
Size: 219 1198 401 1135 681 191 1471
Fragments arranged by size:
1471 1198 1135 681 401 219 191
SfoI GGC'GCC
Cuts at: 2062 2102 2062
Size: 40 5256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5296
SmaI CCC'GGG
Cuts at: 1872 2623 1872
Size: 751 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 3400 4301 4563 820
Size: 197 926 393 1064 901 262 1553
Fragments arranged by size:
1553 1064 926 901 393 262 197
SnaBI TAC'GTA
Cuts at: 493 3087 493
Size: 2594 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5296
SphI G_CATG'C
Cuts at: 1132 2654 1132
Size: 1522 3774
SspI AAT'ATT
Cuts at: 5 52 3220 5
Size: 47 3168 2081
Fragments arranged by size:
3168 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5296
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 2735 513
Size: 552 939 171 560 3074
Fragments arranged by size:
3074 939 560 552 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2552 3323 3340 3493 3678 4123 325
Size: 1255 972 771 17 153 185 445 1498
Fragments arranged by size:
1498 1255 972 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3542 5254 96
Size: 274 80 33 51 1443 1565 1712 138
Fragments arranged by size:
1712 1565 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2450 2482 2576 2608 5256 5288 2450
Size: 32 94 32 2648 32 2458
Fragments arranged by size:
2648 2458 94 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5296
XbaI T'CTAG_A
Cuts at: 2632 2632
Size: 5296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5296
XmaI C'CCGG_G
Cuts at: 1870 2621 1870
Size: 751 4545
XmnI GAAnn'nnTTC
Cuts at: 3425 3425
Size: 5296
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3104 276
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI XcmI