Restriction map for
pFN21AE9575
(Circular) (Six-base) MAPSORT of: pFN21AE9575.seq Check: 8987 from: 1 to: 5782
pFN21AE9575 5782 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2534 2756 2534
Size: 222 5560
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2538 3592 278
Size: 53 83 186 936 1002 1054 2468
Fragments arranged by size:
2468 1054 1002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3048 3845 5229 5497 1632
Size: 1416 797 1384 268 1917
Fragments arranged by size:
1917 1416 1384 797 268
AfeI AGC'GCT
Cuts at: 2366 2402 2366
Size: 36 5746
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2322 2823 4681 5658 1203
Size: 1119 501 1858 977 1327
Fragments arranged by size:
1858 1327 1119 977 501
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2999 1166
Size: 1833 3949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 5750
AlwNI CAG_nnn'CTG
Cuts at: 2053 3525 5097 2053
Size: 1472 1572 2738
Fragments arranged by size:
2738 1572 1472
ApaI G_GGCC'C
Cuts at: 2166 2166
Size: 5782
ApaLI G'TGCA_C
Cuts at: 2504 3839 4995 2504
Size: 1335 1156 3291
Fragments arranged by size:
3291 1335 1156
ApoI r'AATT_y
Cuts at: 1493 2900 3091 3317 3469 5578 1493
Size: 1407 191 226 152 2109 1697
Fragments arranged by size:
2109 1697 1407 226 191 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2895 3107 1870
Size: 73 176 776 212 4545
Fragments arranged by size:
4545 776 212 176 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2406 2941 3103 4558 618
Size: 325 217 627 619 535 162 1455 1842
Fragments arranged by size:
1842 1455 627 619 535 325 217 162
BanII G_rGCy'C
Cuts at: 1391 2166 3101 1391
Size: 775 935 4072
Fragments arranged by size:
4072 935 775
BbeI G_GCGC'C
Cuts at: 2410 2410
Size: 5782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2999 3033 3973 4007 1903
Size: 34 50 34 978 34 940 34 3678
Fragments arranged by size:
3678 978 940 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 2190 2625 1548
Size: 642 435 4705
Fragments arranged by size:
4705 642 435
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 4393 136
Size: 107 122 71 631 605 901 1820 1525
Fragments arranged by size:
1820 1525 901 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 2390 3199 2390
Size: 809 4973
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2508 2946 3843 4999 1790
Size: 376 342 438 897 1156 2573
Fragments arranged by size:
2573 1156 897 438 376 342
BmgBI CAC'GTC
Cuts at: 1318 2997 1318
Size: 1679 4103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2278 3513 4471
Size: 416 53 83 399 24 861 1235 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1235 958 861 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3044 4442 1239
Size: 1805 1398 2579
Fragments arranged by size:
2579 1805 1398
Bpu10I CC'TnA_GC
Cuts at: 2198 2198
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2642 3907 4772 5070 2525
Size: 117 1265 865 298 3237
Fragments arranged by size:
3237 1265 865 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2824 3573 493
Size: 711 1620 749 2702
Fragments arranged by size:
2702 1620 749 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2407 2535 3589 3971
Size: 53 83 186 936 874 128 1054 382
Cuts at: 3971 4606 275
Size: 635 1451
Fragments arranged by size:
1451 1054 936 874 635 382 186 128
83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3062 3092 1178
Size: 30 165 30 1659 30 3868
Fragments arranged by size:
3868 1659 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 1186
Size: 1504 4278
BseYI C'CCAG_C
Cuts at: 2558 4985 5481 2558
Size: 2427 496 2859
Fragments arranged by size:
2859 2427 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2457 1163
Size: 573 721 4488
Fragments arranged by size:
4488 721 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2689 3232 3993 5021 1540
Size: 275 186 688 543 761 1028 2301
Fragments arranged by size:
2301 1028 761 688 543 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2775 3843 3928 4999 2508
Size: 267 1068 85 1071 3291
Fragments arranged by size:
3291 1071 1068 267 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2508 2775 2946 3101 3843 3928
Size: 399 376 342 267 171 155 742 85
Cuts at: 3928 4999 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 342 267 171
155 85
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 2187 3671 5401 2187
Size: 1484 1730 2568
Fragments arranged by size:
2568 1730 1484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1999 2534 2756 3137 844
Size: 1155 535 222 381 3489
Fragments arranged by size:
3489 1155 535 381 222
BsrBI CCG'CTC
Cuts at: 1394 1823 2389 3669 1394
Size: 429 566 1280 3507
Fragments arranged by size:
3507 1280 566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2251 4277 4451 65
Size: 1183 1003 2026 174 1396
Fragments arranged by size:
2026 1396 1183 1003 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 2151 3014 4426 4613 1700
Size: 110 214 127 863 1412 187 2869
Fragments arranged by size:
2869 1412 863 214 187 127 110
BsrGI T'GTAC_A
Cuts at: 96 2826 96
Size: 2730 3052
BssHII G'CGCG_C
Cuts at: 1925 2480 2704 1925
Size: 555 224 5003
Fragments arranged by size:
5003 555 224
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 794
Size: 1259 4523
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 2299 1184
Size: 1115 4667
BstYI r'GATC_y
Cuts at: 1972 2168 3112 3479 3864 3881 5333 5419 5431
Size: 196 944 367 385 17 1452 86 12
Cuts at: 5431 5777 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 944 385 367 346 196 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2085 2304 2631 2721 513
Size: 552 1020 219 327 90 3574
Fragments arranged by size:
3574 1020 552 327 219 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 3298 4092 4112 3298
Size: 794 20 4968
Fragments arranged by size:
4968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DraIII CAC_nnn'GTG
Cuts at: 2788 2841 2788
Size: 53 5729
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2467 4789 809
Size: 507 1151 2322 1802
Fragments arranged by size:
2322 1802 1151 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2427 2565
Size: 54 1425 50 275 18 390 207 138
Cuts at: 2565 2646 2686 2718 3229 4118 5747 8
Size: 81 40 32 511 889 1629 43
Fragments arranged by size:
1629 1425 889 511 390 275 207 138
81 54 50 43 40 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2686 3229 1537
Size: 275 874 543 4090
Fragments arranged by size:
4090 874 543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 2591 2984 3712 1353
Size: 782 456 393 728 3423
Fragments arranged by size:
3423 782 728 456 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2974 4369 4743 4889 387
Size: 1064 138 1385 1395 374 146 1280
Fragments arranged by size:
1395 1385 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3044 3048 3845 4442 5229 5497 1239
Size: 393 1412 4 797 597 787 268 1524
Fragments arranged by size:
1524 1412 797 787 597 393 268 4
EcoO109I rG'GnC_Cy
Cuts at: 2011 2162 2377 2442 2011
Size: 151 215 65 5351
Fragments arranged by size:
5351 215 151 65
EcoRI G'AATT_C
Cuts at: 2900 3091 3469 2900
Size: 191 378 5213
Fragments arranged by size:
5213 378 191
FspI TGC'GCA
Cuts at: 2669 3021 4288 2669
Size: 352 1267 4163
Fragments arranged by size:
4163 1267 352
FspAI rTGC'GCAy
Cuts at: 3021 3021
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2368 2404 2410 4929 1678
Size: 690 36 6 2519 2531
Fragments arranged by size:
2531 2519 690 36 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373
Size: 32 135 32 2851 32 42 32 2626
Fragments arranged by size:
2851 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KasI G'GCGC_C
Cuts at: 2406 2406
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 2567 10
Size: 54 1425 343 390 345 3225
Fragments arranged by size:
3225 1425 390 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2999 3740 4099 4258 518
Size: 648 1020 813 741 359 159 2042
Fragments arranged by size:
2042 1020 813 741 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2476 2698 2723 2850 2908 3198 3875 5023
Size: 870 222 25 127 58 290 677 1148
Cuts at: 5023 5268 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 870 677 290 245 222 127
58 25
NaeI GCC'GGC
Cuts at: 2153 2153
Size: 5782
NarI GG'CG_CC
Cuts at: 2407 2407
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NgoMIV G'CCGG_C
Cuts at: 2151 2151
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2326 3140 4685 1132
Size: 97 1097 814 1545 2229
Fragments arranged by size:
2229 1545 1097 814 97
PasI CC'CwG_GG
Cuts at: 1348 2512 1348
Size: 1164 4618
PciI A'CATG_T
Cuts at: 2322 4681 2322
Size: 2359 3423
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2403 1108
Size: 1295 4487
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PmlI CAC'GTG
Cuts at: 2824 2824
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3851 3883 5364 5396 3062
Size: 32 757 32 1481 32 3448
Fragments arranged by size:
3448 1481 757 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2377 2442 2011
Size: 366 65 5351
Fragments arranged by size:
5351 366 65
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PspOMI G'GGCC_C
Cuts at: 2162 2162
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2049 3134 830
Size: 1219 1085 3478
Fragments arranged by size:
3478 1219 1085
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2850 2908 2850
Size: 58 5724
SacII CC_GC'GG
Cuts at: 2724 2724
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2045 3130 4265 4946 5137 826
Size: 219 1000 1085 1135 681 191 1471
Fragments arranged by size:
1471 1135 1085 1000 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2573 2573
Size: 5782
SfoI GGC'GCC
Cuts at: 2408 2408
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 2121 2897 3109 1872
Size: 249 776 212 4545
Fragments arranged by size:
4545 776 249 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 2657 3886 4787 5049 820
Size: 197 926 597 117 1229 901 262 1553
Fragments arranged by size:
1553 1229 926 901 597 262 197 117
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 2924 3706 5
Size: 47 2872 782 2081
Fragments arranged by size:
2872 2081 782 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 3221 513
Size: 552 2156 3074
Fragments arranged by size:
3074 2156 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2131 2167 3809 3826 3979 4164 4609
Size: 1255 551 36 1642 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1642 1498 1255 551 445 185 153 36
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 2826 4028
Size: 274 80 33 51 1443 114 735 1202
Cuts at: 4028 5740 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1202 735 274 138 114 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 2995 1316
Size: 1679 4103
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2953 2953
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 2119 2895 3107 1870
Size: 249 776 212 4545
Fragments arranged by size:
4545 776 249 212
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2536 3590 276
Size: 53 83 186 936 1002 1054 2468
Fragments arranged by size:
2468 1054 1002 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SanDI SapI SexAI SrfI SwaI