Restriction map for
pFN21AE9574
(Circular) (Six-base) MAPSORT of: pFN21AE9574.seq Check: 4533 from: 1 to: 5755
pFN21AE9574 5755 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2997 3818 5202 5470 1632
Size: 1365 821 1384 268 1917
Fragments arranged by size:
1917 1384 1365 821 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2361 2967 4654 5631 1203
Size: 1158 606 1687 977 1327
Fragments arranged by size:
1687 1327 1158 977 606
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 2078 1166
Size: 912 4843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2354 1184
Size: 1170 4585
AlwNI CAG_nnn'CTG
Cuts at: 3498 5070 3498
Size: 1572 4183
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2433 3076 4531 618
Size: 325 217 627 646 643 1455 1842
Fragments arranged by size:
1842 1455 646 643 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3074 1391
Size: 1683 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2137 3047 928
Size: 971 238 910 3636
Fragments arranged by size:
3636 971 910 238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2050 3647 4857 2050
Size: 1597 1210 2948
Fragments arranged by size:
2948 1597 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2459 4366 136
Size: 107 122 71 631 605 787 1907 1525
Fragments arranged by size:
1907 1525 787 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2043 3816 4972 1790
Size: 253 1773 1156 2573
Fragments arranged by size:
2573 1773 1156 253
BmgBI CAC'GTC
Cuts at: 1318 2286 2691 1318
Size: 968 405 4382
Fragments arranged by size:
4382 968 405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2828 4415 1239
Size: 1589 1587 2579
Fragments arranged by size:
2579 1589 1587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 3880 4745 5043 2711
Size: 1169 865 298 3423
Fragments arranged by size:
3423 1169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2905 3546 493
Size: 711 1701 641 2702
Fragments arranged by size:
2702 1701 711 641
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2685 2961 4188 4586 4860 5007 5418 2685
Size: 276 1227 398 274 147 411 3022
Fragments arranged by size:
3022 1227 411 398 276 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2270 2801 2831 2987
Size: 30 165 30 837 30 531 30 156
Cuts at: 2987 3017 1178
Size: 30 3916
Fragments arranged by size:
3916 837 531 165 156 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2555 2948 1186
Size: 1369 393 3993
Fragments arranged by size:
3993 1369 393
BseYI C'CCAG_C
Cuts at: 2161 2951 4958 5454 2161
Size: 790 2007 496 2462
Fragments arranged by size:
2462 2007 790 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2404 3205 3966 4994 1540
Size: 275 186 403 801 761 1028 2301
Fragments arranged by size:
2301 1028 801 761 403 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 2192 3266 3359 2192
Size: 1074 93 4588
Fragments arranged by size:
4588 1074 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 3074 3816 3901 4972 1391
Size: 399 253 1031 742 85 1071 2174
Fragments arranged by size:
2174 1071 1031 742 399 253 85
BspEI T'CCGG_A
Cuts at: 2685 2961 5418 2685
Size: 276 2457 3022
Fragments arranged by size:
3022 2457 276
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 844
Size: 2266 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2173 4250 4424 65
Size: 1183 925 2077 174 1396
Fragments arranged by size:
2077 1396 1183 925 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2453 2912 4399 4586 1700
Size: 110 643 459 1487 187 2869
Fragments arranged by size:
2869 1487 643 459 187 110
BsrGI T'GTAC_A
Cuts at: 96 2293 96
Size: 2197 3558
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750
Size: 1113 367 385 17 1452 86 12 346
Cuts at: 5750 1972
Size: 1977
Fragments arranged by size:
1977 1452 1113 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2478 2607 2985 513
Size: 552 1413 129 378 3283
Fragments arranged by size:
3283 1413 552 378 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2748 3057 3271 4065 4085 2748
Size: 309 214 794 20 4418
Fragments arranged by size:
4418 794 309 214 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2980 4762 809
Size: 507 1664 1782 1802
Fragments arranged by size:
1802 1782 1664 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 2610 2748
Size: 54 1425 50 275 18 555 225 138
Cuts at: 2748 2910 2914 3202 4091 5720 8
Size: 162 4 288 889 1629 43
Fragments arranged by size:
1629 1425 889 555 288 275 225 162
138 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2973 3685 1353
Size: 1620 712 3423
Fragments arranged by size:
3423 1620 712
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2828 2997 3818 4415 5202 5470 1239
Size: 393 1196 169 821 597 787 268 1524
Fragments arranged by size:
1524 1196 821 787 597 393 268 169
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FseI GG_CCGG'CC
Cuts at: 2916 2916
Size: 5755
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2270 2400 4902 1678
Size: 592 130 2502 2531
Fragments arranged by size:
2531 2502 592 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 2801 2833 2987
Size: 32 135 32 668 32 529 32 154
Cuts at: 2987 3019 4396 4428 4470 4502 1373
Size: 32 1377 32 42 32 2626
Fragments arranged by size:
2626 1377 668 529 154 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3099 3345 669
Size: 988 281 1035 126 246 3079
Fragments arranged by size:
3079 1035 988 281 246 126
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 2612 2750 10
Size: 54 1425 343 555 225 138 3015
Fragments arranged by size:
3015 1425 555 343 225 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2046 2078 2903 3713 4072 4231 518
Size: 648 880 32 825 810 359 159 2042
Fragments arranged by size:
2042 880 825 810 648 359 159 32
MspA1I CmG'CkG
Cuts at: 1606 3171 3848 4996 5241 1606
Size: 1565 677 1148 245 2120
Fragments arranged by size:
2120 1565 1148 677 245
NaeI GCC'GGC
Cuts at: 2455 2914 2455
Size: 459 5296
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5203
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NgoMIV G'CCGG_C
Cuts at: 2453 2912 2453
Size: 459 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2365 3113 4658 1132
Size: 97 1136 748 1545 2229
Fragments arranged by size:
2229 1545 1136 748 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 2361 4654 2361
Size: 2293 3462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3052 1108
Size: 1944 3811
PfoI T'CCnGG_A
Cuts at: 2535 2805 2535
Size: 270 5485
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2333 3107 830
Size: 1503 774 3478
Fragments arranged by size:
3478 1503 774
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 2546 4003 5715 2546
Size: 1457 1712 2586
Fragments arranged by size:
2586 1712 1457
SexAI A'CCwGG_T
Cuts at: 2778 2778
Size: 5755
SfcI C'TryA_G
Cuts at: 826 1045 2329 2578 3103 4238 4919 5110 826
Size: 219 1284 249 525 1135 681 191 1471
Fragments arranged by size:
1471 1284 1135 681 525 249 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2459 2459
Size: 5755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 3859 4760 5022 820
Size: 197 926 783 1133 901 262 1553
Fragments arranged by size:
1553 1133 926 901 783 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2124 3194 513
Size: 552 1059 1070 3074
Fragments arranged by size:
3074 1070 1059 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 2836 3782 3799 3952 4137 4582
Size: 1255 593 663 946 17 153 185 445
Cuts at: 4582 325
Size: 1498
Fragments arranged by size:
1498 1255 946 663 593 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2293 2544 4001
Size: 274 80 33 51 1443 316 251 1457
Cuts at: 4001 5713 96
Size: 1712 138
Fragments arranged by size:
1712 1457 1443 316 274 251 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2458 2490 2811 2840 2843 2872 5715 5747 2458
Size: 32 321 29 3 29 2843 32 2466
Fragments arranged by size:
2843 2466 321 32 32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2312 2620 2902 2312
Size: 308 282 5165
Fragments arranged by size:
5165 308 282
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 3884 2012
Size: 1872 3883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfoI SrfI SwaI