Restriction map for
pFN21AE9571
(Circular) (Six-base) MAPSORT of: pFN21AE9571.seq Check: 8437 from: 1 to: 5929
pFN21AE9571 5929 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3739 278
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2673 3272 1937
Size: 736 599 4594
Fragments arranged by size:
4594 736 599
Acc65I G'GTAC_C
Cuts at: 3250 3250
Size: 5929
AclI AA'CG_TT
Cuts at: 1563 4056 4429 1563
Size: 2493 373 3063
Fragments arranged by size:
3063 2493 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3992 5376 5644 1632
Size: 2360 1384 268 1917
Fragments arranged by size:
2360 1917 1384 268
AfeI AGC'GCT
Cuts at: 2459 2567 2459
Size: 108 5821
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5732
AflIII A'CryG_T
Cuts at: 1203 2223 4828 5805 1203
Size: 1020 2605 977 1327
Fragments arranged by size:
2605 1327 1020 977
AgeI A'CCGG_T
Cuts at: 4760 4760
Size: 5929
AhdI GACnn_n'nnGTC
Cuts at: 4658 4658
Size: 5929
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5929
AlwNI CAG_nnn'CTG
Cuts at: 3672 5244 3672
Size: 1572 4357
ApaI G_GGCC'C
Cuts at: 2131 2875 2963 2981 2131
Size: 744 88 18 5079
Fragments arranged by size:
5079 744 88 18
ApaLI G'TGCA_C
Cuts at: 2878 3986 5142 2878
Size: 1108 1156 3665
Fragments arranged by size:
3665 1156 1108
ApoI r'AATT_y
Cuts at: 1493 3238 3464 3616 5725 1493
Size: 1745 226 152 2109 1697
Fragments arranged by size:
2109 1745 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4483 160
Size: 872 3451 1606
Fragments arranged by size:
3451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
AvaI C'yCGr_G
Cuts at: 1870 1943 2744 2974 3148 3254 1870
Size: 73 801 230 174 106 4545
Fragments arranged by size:
4545 801 230 174 106 73
BamHI G'GATC_C
Cuts at: 3259 3626 3259
Size: 367 5562
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2275 2321 2761 2818
Size: 325 217 627 278 210 46 440 57
Cuts at: 2818 2900 3250 4705 618
Size: 82 350 1455 1842
Fragments arranged by size:
1842 1455 627 440 350 325 278 217
210 82 57 46
BanII G_rGCy'C
Cuts at: 1391 2131 2875 2963 2981 3117 3248 1391
Size: 740 744 88 18 136 131 4072
Fragments arranged by size:
4072 744 740 136 131 88 18
BbeI G_GCGC'C
Cuts at: 2069 2325 2765 2069
Size: 256 440 5233
Fragments arranged by size:
5233 440 256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3071 928
Size: 971 1172 3786
Fragments arranged by size:
3786 1172 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4120 4154 1903
Size: 34 2183 34 3678
Fragments arranged by size:
3678 2183 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3821 5031 3821
Size: 1210 4719
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5929
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 2294 3093
Size: 107 122 71 631 605 463 159 799
Cuts at: 3093 3134 4540 136
Size: 41 1406 1525
Fragments arranged by size:
1525 1406 799 631 605 463 159 122
107 71 41
BglII A'GATC_T
Cuts at: 5924 5924
Size: 5929
BlpI GC'TnA_GC
Cuts at: 3346 3346
Size: 5929
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2823 2875 2882 2903 2963 2981 3990
Size: 341 692 52 7 21 60 18 1009
Cuts at: 3990 5146 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1009 692 341 60 52 21
18 7
BmgBI CAC'GTC
Cuts at: 1318 2356 2799 1318
Size: 1038 443 4448
Fragments arranged by size:
4448 1038 443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 4618 442
Size: 416 53 83 399 24 2243 958 1753
Fragments arranged by size:
2243 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5929
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 4589 1239
Size: 953 2397 2579
Fragments arranged by size:
2579 2397 953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4054 4919 5217 4054
Size: 865 298 4766
Fragments arranged by size:
4766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2894 4592 882
Size: 617 1395 1698 2219
Fragments arranged by size:
2219 1698 1395 617
BsaAI yAC'GTr
Cuts at: 493 1204 2617 3720 493
Size: 711 1413 1103 2702
Fragments arranged by size:
2702 1413 1103 711
BsaBI GATnn'nnATC
Cuts at: 5923 5923
Size: 5929
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2322 2553 2762
Size: 53 83 186 936 533 256 231 209
Cuts at: 2762 3736 4118 4753 275
Size: 974 382 635 1451
Fragments arranged by size:
1451 974 936 635 533 382 256 231
209 186 83 53
BsaWI w'CCGG_w
Cuts at: 3109 4362 4760 5034 5181 5592 3109
Size: 1253 398 274 147 411 3446
Fragments arranged by size:
3446 1253 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2788 2818 3213 3243 1178
Size: 30 165 30 1385 30 395 30 3864
Fragments arranged by size:
3864 1385 395 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2620 2762 1186
Size: 1434 142 4353
Fragments arranged by size:
4353 1434 142
BseYI C'CCAG_C
Cuts at: 2728 3175 5132 5628 2728
Size: 447 1957 496 3029
Fragments arranged by size:
3029 1957 496 447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2502 2833 3097 3379 4140 5168
Size: 275 186 501 331 264 282 761 1028
Cuts at: 5168 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 501 331 282 275 264
186
BsiHKAI G_wGCw'C
Cuts at: 2882 3990 4075 5146 2882
Size: 1108 85 1071 3665
Fragments arranged by size:
3665 1108 1071 85
BsmI GAATG_Cn'
Cuts at: 3440 3533 3440
Size: 93 5836
BsmBI CGTCTCn'nnnn_
Cuts at: 2641 2641
Size: 5929
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2823 2875 2882 2903 2963 2981
Size: 399 341 692 52 7 21 60 18
Cuts at: 2981 3117 3248 3990 4075 5146 1391
Size: 136 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 692 399 341 136 131
85 60 52 21 18 7
BspEI T'CCGG_A
Cuts at: 3109 5592 3109
Size: 2483 3446
BspHI T'CATG_A
Cuts at: 2523 3818 5548 2523
Size: 1295 1730 2904
Fragments arranged by size:
2904 1730 1295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 3284 844
Size: 1334 1106 3489
Fragments arranged by size:
3489 1334 1106
BsrBI CCG'CTC
Cuts at: 1394 1823 3034 3816 1394
Size: 429 1211 782 3507
Fragments arranged by size:
3507 1211 782 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4424 4598 65
Size: 1183 3176 174 1396
Fragments arranged by size:
3176 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2281 2758 2832 4573 4760 1700
Size: 110 471 477 74 1741 187 2869
Fragments arranged by size:
2869 1741 477 471 187 110 74
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5929
BssHII G'CGCG_C
Cuts at: 1925 2843 2947 1925
Size: 918 104 4907
Fragments arranged by size:
4907 918 104
BssSI C'ACGA_G
Cuts at: 2021 2657 3989 5001 2021
Size: 636 1332 1012 2949
Fragments arranged by size:
2949 1332 1012 636
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5929
BstBI TT'CG_AA
Cuts at: 4733 4733
Size: 5929
BstEII G'GTnAC_C
Cuts at: 1184 2449 1184
Size: 1265 4664
BstYI r'GATC_y
Cuts at: 1972 3259 3626 4011 4028 5480 5566 5578 5924
Size: 1287 367 385 17 1452 86 12 346
Cuts at: 5924 1972
Size: 1977
Fragments arranged by size:
1977 1452 1287 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2042 2105 2157 2400 2502
Size: 552 939 8 30 63 52 243 102
Cuts at: 2502 513
Size: 3940
Fragments arranged by size:
3940 939 552 243 102 63 52 30
8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2173 487
Size: 683 56 947 4243
Fragments arranged by size:
4243 947 683 56
BtsI GCAGTG_nn'
Cuts at: 3445 4239 4259 3445
Size: 794 20 5115
Fragments arranged by size:
5115 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5929
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2068 2103 529
Size: 35 1504 35 4355
Fragments arranged by size:
4355 1504 35 35
DraI TTT'AAA
Cuts at: 3233 3233
Size: 5929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4936 809
Size: 507 3620 1802
Fragments arranged by size:
3620 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 2151 2184
Size: 54 1425 50 275 18 215 106 33
Cuts at: 2184 2241 2286 2305 2312 2397 2499 2830 2934
Size: 57 45 19 7 85 102 331 104
Cuts at: 2934 3094 3376 4265 5894 8
Size: 160 282 889 1629 43
Fragments arranged by size:
1629 1425 889 331 282 275 215 160
106 104 102 85 57 54 50 45
43 33 19 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2499 2830 3094 3376 1537
Size: 275 687 331 264 282 4090
Fragments arranged by size:
4090 687 331 282 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 2658 2672 3146 3859 1353
Size: 1305 14 474 713 3423
Fragments arranged by size:
3423 1305 713 474 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2730 2745 2796 3062 4516 4890
Size: 1064 138 1141 15 51 266 1454 374
Cuts at: 4890 5036 387
Size: 146 1280
Fragments arranged by size:
1454 1280 1141 1064 374 266 146 138
51 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 3992 4589 5376 5644 1239
Size: 393 560 1800 597 787 268 1524
Fragments arranged by size:
1800 1524 787 597 560 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2127 2128 2872 2959 2960 2127
Size: 1 744 87 1 5096
Fragments arranged by size:
5096 744 87 1 1
EcoRI G'AATT_C
Cuts at: 3238 3616 3238
Size: 378 5551
FspI TGC'GCA
Cuts at: 4435 4435
Size: 5929
HaeII r_GCGC'y
Cuts at: 1678 2069 2141 2300 2325 2461 2569 2765 3069
Size: 391 72 159 25 136 108 196 304
Cuts at: 3069 5076 1678
Size: 2007 2531
Fragments arranged by size:
2531 2007 391 304 196 159 136 108
72 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2788 2820 4570 4602 4644
Size: 32 135 32 1216 32 1750 32 42
Cuts at: 4644 4676 1373
Size: 32 2626
Fragments arranged by size:
2626 1750 1216 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3273 3519 669
Size: 988 281 1335 246 3079
Fragments arranged by size:
3079 1335 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5929
HpaI GTT'AAC
Cuts at: 3519 3519
Size: 5929
KasI G'GCGC_C
Cuts at: 2065 2321 2761 2065
Size: 256 440 5233
Fragments arranged by size:
5233 440 256
KpnI G_GTAC'C
Cuts at: 3254 3254
Size: 5929
MfeI C'AATT_G
Cuts at: 3528 3528
Size: 5929
MluI A'CGCG_T
Cuts at: 5805 5805
Size: 5929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2601 2628 5043 5227 1305
Size: 1296 27 2415 184 2007
Fragments arranged by size:
2415 2007 1296 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2153 2186 2307 2936 10
Size: 54 1425 343 321 33 121 629 3003
Fragments arranged by size:
3003 1425 629 343 321 121 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 3887 4246 4405 518
Size: 648 2721 359 159 2042
Fragments arranged by size:
2721 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2431 2474 2504 2707 3090 3345 4022 5170
Size: 825 43 30 203 383 255 677 1148
Cuts at: 5170 5415 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 825 677 383 255 245 203
43 30
NaeI GCC'GGC
Cuts at: 2283 2760 2834 2283
Size: 477 74 5378
Fragments arranged by size:
5378 477 74
NarI GG'CG_CC
Cuts at: 2066 2322 2762 2066
Size: 256 440 5233
Fragments arranged by size:
5233 440 256
NcoI C'CATG_G
Cuts at: 513 1065 2004 2042 513
Size: 552 939 38 4400
Fragments arranged by size:
4400 939 552 38
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5929
NgoMIV G'CCGG_C
Cuts at: 2281 2758 2832 2281
Size: 477 74 5378
Fragments arranged by size:
5378 477 74
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5929
NotI GC'GGCC_GC
Cuts at: 3376 3376
Size: 5929
NspI r_CATG'y
Cuts at: 1132 1229 2611 3287 4832 1132
Size: 97 1382 676 1545 2229
Fragments arranged by size:
2229 1545 1382 676 97
PasI CC'CwG_GG
Cuts at: 1348 2953 1348
Size: 1605 4324
PciI A'CATG_T
Cuts at: 4828 4828
Size: 5929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2193 1108
Size: 1085 4844
PfoI T'CCnGG_A
Cuts at: 2859 2859
Size: 5929
PmeI GTTT'AAAC
Cuts at: 3233 3233
Size: 5929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 5511 5543 3998
Size: 32 1481 32 4384
Fragments arranged by size:
4384 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5929
PsiI TTA'TAA
Cuts at: 3499 3499
Size: 5929
PspOMI G'GGCC_C
Cuts at: 2127 2871 2959 2977 2127
Size: 744 88 18 5079
Fragments arranged by size:
5079 744 88 18
PstI C_TGCA'G
Cuts at: 830 3281 830
Size: 2451 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5929
PvuII CAG'CTG
Cuts at: 2474 2707 2474
Size: 233 5696
SacII CC_GC'GG
Cuts at: 2505 2505
Size: 5929
SalI G'TCGA_C
Cuts at: 1936 3271 1936
Size: 1335 4594
SapI GCTCTTCn'nnn_
Cuts at: 2672 2672
Size: 5929
SbfI CC_TGCA'GG
Cuts at: 3281 3281
Size: 5929
ScaI AGT'ACT
Cuts at: 4177 5889 4177
Size: 1712 4217
SexAI A'CCwGG_T
Cuts at: 2253 2253
Size: 5929
SfcI C'TryA_G
Cuts at: 826 1045 3277 4412 5093 5284 826
Size: 219 2232 1135 681 191 1471
Fragments arranged by size:
2232 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2067 2323 2763 2067
Size: 256 440 5233
Fragments arranged by size:
5233 440 256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
SgrAI Cr'CCGG_yG
Cuts at: 1700 2281 2758 1700
Size: 581 477 4871
Fragments arranged by size:
4871 581 477
SmaI CCC'GGG
Cuts at: 1872 2976 3256 1872
Size: 1104 280 4545
Fragments arranged by size:
4545 1104 280
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4033 4934 5196 820
Size: 197 926 2090 901 262 1553
Fragments arranged by size:
2090 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3720 493
Size: 3227 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5929
SphI G_CATG'C
Cuts at: 1132 3287 1132
Size: 2155 3774
SspI AAT'ATT
Cuts at: 5 52 3853 5
Size: 47 3801 2081
Fragments arranged by size:
3801 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5929
StyI C'CwwG_G
Cuts at: 513 1065 2004 2042 2292 2883 3368 513
Size: 552 939 38 250 591 485 3074
Fragments arranged by size:
3074 939 591 552 485 250 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3956 3973 4126 4311 4756 325
Size: 1255 2376 17 153 185 445 1498
Fragments arranged by size:
2376 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4175 5887 96
Size: 274 80 33 51 1443 2198 1712 138
Fragments arranged by size:
2198 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2256 2288 2345 2377 5889 5921 2256
Size: 32 57 32 3512 32 2264
Fragments arranged by size:
3512 2264 57 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5929
XbaI T'CTAG_A
Cuts at: 3265 3265
Size: 5929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2103 2103
Size: 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5929
XmaI C'CCGG_G
Cuts at: 1870 2974 3254 1870
Size: 1104 280 4545
Fragments arranged by size:
4545 1104 280
XmnI GAAnn'nnTTC
Cuts at: 4058 4058
Size: 5929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3737 276
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SfiI SrfI SwaI