Restriction map for
pFN21AE9569
(Circular) (Six-base) MAPSORT of: pFN21AE9569.seq Check: 3139 from: 1 to: 5866
pFN21AE9569 5866 bp
With 182 enzymes: SgfI *
November 11, 2011 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2658 3209 1937
Size: 721 551 4594
Fragments arranged by size:
4594 721 551
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2569 3929 5313 5581 1632
Size: 937 1360 1384 268 1917
Fragments arranged by size:
1917 1384 1360 937 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 2138 1166
Size: 972 4894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2321 1184
Size: 1137 4729
AlwNI CAG_nnn'CTG
Cuts at: 3609 5181 3609
Size: 1572 4294
ApaI G_GGCC'C
Cuts at: 2481 3058 3128 2481
Size: 577 70 5219
Fragments arranged by size:
5219 577 70
ApaLI G'TGCA_C
Cuts at: 2306 3923 5079 2306
Size: 1617 1156 3093
Fragments arranged by size:
3093 1617 1156
ApoI r'AATT_y
Cuts at: 1493 3175 3401 3553 5662 1493
Size: 1682 226 152 2109 1697
Fragments arranged by size:
2109 1697 1682 226 152
AscI GG'CGCG_CC
Cuts at: 2650 2689 2650
Size: 39 5827
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2072 2677 2959 3095 3191 1870
Size: 73 71 58 605 282 136 96 4545
Fragments arranged by size:
4545 605 282 136 96 73 71 58
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2067 2120 2681 2987 3187
Size: 325 217 627 280 53 561 306 200
Cuts at: 3187 4642 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 561 325 306 280 217
200 53
BanII G_rGCy'C
Cuts at: 1391 2448 2481 2678 3058 3102 3128 3185 1391
Size: 1057 33 197 380 44 26 57 4072
Fragments arranged by size:
4072 1057 380 197 57 44 33 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2575 928
Size: 971 676 4219
Fragments arranged by size:
4219 971 676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BfrBI ATG'CAT
Cuts at: 3090 3090
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2937 4477
Size: 107 122 71 631 605 377 888 1540
Cuts at: 4477 136
Size: 1525
Fragments arranged by size:
1540 1525 888 631 605 377 122 107
71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2123 2244 2310 2481 2686 2947 2990
Size: 301 32 121 66 171 205 261 43
Cuts at: 2990 3058 3128 3927 5083 1790
Size: 68 70 799 1156 2573
Fragments arranged by size:
2573 1156 799 301 261 205 171 121
70 68 66 43 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2565 2565
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4526 1239
Size: 3287 2579
Bpu10I CC'TnA_GC
Cuts at: 2486 2486
Size: 5866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2848 2893 4529 882
Size: 617 1349 45 1636 2219
Fragments arranged by size:
2219 1636 1349 617 45
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 2720 5860 2720
Size: 3140 2726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4299 4697 4971 5118 5529 4299
Size: 398 274 147 411 4636
Fragments arranged by size:
4636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2828 2840 2858 2870 3002
Size: 30 165 30 1425 12 18 12 132
Cuts at: 3002 3032 1178
Size: 30 4012
Fragments arranged by size:
4012 1425 165 132 30 30 30 18
12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5866
BseYI C'CCAG_C
Cuts at: 2274 2935 3081 5069 5565 2274
Size: 661 146 1988 496 2575
Fragments arranged by size:
2575 1988 661 496 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 2557 1163
Size: 573 645 176 4472
Fragments arranged by size:
4472 645 573 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2310 3927 4012 5083 2310
Size: 1617 85 1071 3093
Fragments arranged by size:
3093 1617 1071 85
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
BsmBI CGTCTCn'nnnn_
Cuts at: 2017 2365 2017
Size: 348 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2123 2244 2310 2448 2481 2678
Size: 399 301 32 121 66 138 33 197
Cuts at: 2678 2686 2947 2990 3058 3102 3128 3185 3927
Size: 8 261 43 68 44 26 57 742
Cuts at: 3927 4012 5083 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 301 261 197 138
121 85 68 66 57 44 43 33
32 26 8
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 3221 844
Size: 1757 620 3489
Fragments arranged by size:
3489 1757 620
BsrBI CCG'CTC
Cuts at: 1394 1823 2800 3753 1394
Size: 429 977 953 3507
Fragments arranged by size:
3507 977 953 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4361 4535 65
Size: 1183 3113 174 1396
Fragments arranged by size:
3113 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4510 4697 1700
Size: 110 2700 187 2869
Fragments arranged by size:
2869 2700 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 2627 2650 2689 2806 1925
Size: 702 23 39 117 4985
Fragments arranged by size:
4985 702 117 39 23
BssSI C'ACGA_G
Cuts at: 2114 3926 4938 2114
Size: 1812 1012 3042
Fragments arranged by size:
3042 1812 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2922 794
Size: 2128 3738
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 3196 3563 3948 3965 5417 5503 5515 5861
Size: 1224 367 385 17 1452 86 12 346
Cuts at: 5861 1972
Size: 1977
Fragments arranged by size:
1977 1452 1224 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2124 2562 2670 513
Size: 552 1059 438 108 3709
Fragments arranged by size:
3709 1059 552 438 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2164 2211 2352
Size: 54 1425 50 275 18 334 47 141
Cuts at: 2352 2379 2511 2667 2727 2788 3162 3313 4202
Size: 27 132 156 60 61 374 151 889
Cuts at: 4202 5831 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 374 334 275 156 151
141 132 61 60 54 50 47 43
27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2474 2741 3796 1353
Size: 749 372 267 1055 3423
Fragments arranged by size:
3423 1055 749 372 267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2569 3929 4526 5313 5581 1239
Size: 393 937 1360 597 787 268 1524
Fragments arranged by size:
1524 1360 937 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2478 3054 3055 3124 2478
Size: 576 1 69 5220
Fragments arranged by size:
5220 576 69 1
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FspI TGC'GCA
Cuts at: 2414 4372 2414
Size: 1958 3908
HaeII r_GCGC'y
Cuts at: 1678 2547 3078 5013 1678
Size: 869 531 1935 2531
Fragments arranged by size:
2531 1935 869 531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3002 3034 4507 4539 4581
Size: 32 135 32 1430 32 1473 32 42
Cuts at: 4581 4613 1373
Size: 32 2626
Fragments arranged by size:
2626 1473 1430 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2659 2866 3210 3456 669
Size: 988 281 721 207 344 246 3079
Fragments arranged by size:
3079 988 721 344 281 246 207
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2988 4980 5164 1305
Size: 1683 1992 184 2007
Fragments arranged by size:
2007 1992 1683 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 2513 10
Size: 54 1425 343 549 132 3363
Fragments arranged by size:
3363 1425 549 343 132 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 3824 4183 4342 518
Size: 648 972 1686 359 159 2042
Fragments arranged by size:
2042 1686 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2395 2509 2672 3282 3959 5107 5352 1606
Size: 789 114 163 610 677 1148 245 2120
Fragments arranged by size:
2120 1148 789 677 610 245 163 114
NcoI C'CATG_G
Cuts at: 513 1065 2562 513
Size: 552 1497 3817
Fragments arranged by size:
3817 1497 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NsiI A_TGCA'T
Cuts at: 3092 3092
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 3224 4769 1132
Size: 97 1995 1545 2229
Fragments arranged by size:
2229 1995 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2024 1348
Size: 676 5190
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5866
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PspOMI G'GGCC_C
Cuts at: 2477 3054 3124 2477
Size: 577 70 5219
Fragments arranged by size:
5219 577 70
PspXI vC'TCGA_Gb
Cuts at: 3095 3095
Size: 5866
PstI C_TGCA'G
Cuts at: 830 3218 830
Size: 2388 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
PvuII CAG'CTG
Cuts at: 2509 2509
Size: 5866
SacII CC_GC'GG
Cuts at: 2673 2673
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 2657 3208 1936
Size: 721 551 4594
Fragments arranged by size:
4594 721 551
SapI GCTCTTCn'nnn_
Cuts at: 2102 2474 2741 2102
Size: 372 267 5227
Fragments arranged by size:
5227 372 267
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SexAI A'CCwGG_T
Cuts at: 2838 2838
Size: 5866
SfcI C'TryA_G
Cuts at: 826 1045 3214 4349 5030 5221 826
Size: 219 2169 1135 681 191 1471
Fragments arranged by size:
2169 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2937 2937
Size: 5866
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3095 3970 4871 5133 820
Size: 197 926 1152 875 901 262 1553
Fragments arranged by size:
1553 1152 926 901 875 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3224 1132
Size: 2092 3774
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2562 3305 513
Size: 552 1497 743 3074
Fragments arranged by size:
3074 1497 743 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 3005 3893 3910 4063 4248 4693
Size: 1255 686 739 888 17 153 185 445
Cuts at: 4693 325
Size: 1498
Fragments arranged by size:
1498 1255 888 739 686 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4112 5824 96
Size: 274 80 33 51 1443 2135 1712 138
Fragments arranged by size:
2135 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 2828 2860 5826 5858 2700
Size: 32 96 32 2966 32 2708
Fragments arranged by size:
2966 2708 96 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2263
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 3095 1943
Size: 1152 4714
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbeI BbvCI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PsrI RsrII SacI SanDI SfoI
SrfI SwaI