Restriction map for
pFN21AE9568
(Circular) (Six-base) MAPSORT of: pFN21AE9568.seq Check: 1801 from: 1 to: 5899
pFN21AE9568 5899 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3173 3173
Size: 5899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2661 2980 3962 5346 5614 1632
Size: 398 631 319 982 1384 268 1917
Fragments arranged by size:
1917 1384 982 631 398 319 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 4798 5775 1203
Size: 3595 977 1327
Fragments arranged by size:
3595 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 3146 1166
Size: 1980 3919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 2213 3642 5214 2213
Size: 1429 1572 2898
Fragments arranged by size:
2898 1572 1429
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 2922 2981 3208 3434 3586 5695 1493
Size: 1429 59 227 226 152 2109 1697
Fragments arranged by size:
2109 1697 1429 227 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 2331 4453 160
Size: 872 1299 2122 1606
Fragments arranged by size:
2122 1606 1299 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 3224 1870
Size: 73 1281 4545
Fragments arranged by size:
4545 1281 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3220 4675 618
Size: 325 217 627 1433 1455 1842
Fragments arranged by size:
1842 1455 1433 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3218 1391
Size: 1827 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3193 3791 5001 3193
Size: 598 1210 4091
Fragments arranged by size:
4091 1210 598
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BfrBI ATG'CAT
Cuts at: 2336 2336
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4510 136
Size: 107 122 71 631 605 2838 1525
Fragments arranged by size:
2838 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2550 5894 2550
Size: 3344 2555
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 3960 5116 1790
Size: 2170 1156 2573
Fragments arranged by size:
2573 2170 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2398 3630 4588
Size: 416 53 83 399 24 981 1232 958
Cuts at: 4588 442
Size: 1753
Fragments arranged by size:
1753 1232 981 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2539 2866 4559 1239
Size: 1061 239 327 1693 2579
Fragments arranged by size:
2579 1693 1061 327 239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4024 4889 5187 4024
Size: 865 298 4736
Fragments arranged by size:
4736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3690 493
Size: 711 2486 2702
Fragments arranged by size:
2702 2486 711
BsaBI GATnn'nnATC
Cuts at: 2441 5893 2441
Size: 3452 2447
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3706 4088 4723 275
Size: 53 83 186 936 2173 382 635 1451
Fragments arranged by size:
2173 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 1178
Size: 30 165 30 1134 30 4510
Fragments arranged by size:
4510 1134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5899
BseYI C'CCAG_C
Cuts at: 5102 5598 5102
Size: 496 5403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2398 3009 1163
Size: 573 662 611 4053
Fragments arranged by size:
4053 662 611 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3960 4045 5116 3960
Size: 85 1071 4743
Fragments arranged by size:
4743 1071 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3218 3960 4045 5116 1391
Size: 399 1428 742 85 1071 2174
Fragments arranged by size:
2174 1428 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 2154 3788 5518 2154
Size: 1634 1730 2535
Fragments arranged by size:
2535 1730 1634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2782 3046 3173 3254 844
Size: 1938 264 127 81 3489
Fragments arranged by size:
3489 1938 264 127 81
BsrBI CCG'CTC
Cuts at: 1394 1823 3786 1394
Size: 429 1963 3507
Fragments arranged by size:
3507 1963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4394 4568 65
Size: 1183 3146 174 1396
Fragments arranged by size:
3146 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4543 4730 1700
Size: 110 2733 187 2869
Fragments arranged by size:
2869 2733 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 2052 3959 4971 2052
Size: 1907 1012 2980
Fragments arranged by size:
2980 1907 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2925 794
Size: 2131 3768
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 2083 2794 1184
Size: 899 711 4289
Fragments arranged by size:
4289 899 711
BstXI CCAn_nnnn'nTGG
Cuts at: 2806 2806
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 2497 2550 2680 3229 3596 3981 3998 5450
Size: 525 53 130 549 367 385 17 1452
Cuts at: 5450 5536 5548 5894 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 549 525 385 367 346 130
86 53 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4408
Fragments arranged by size:
4408 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 2817 3415 4209 4229 2064
Size: 753 598 794 20 3734
Fragments arranged by size:
3734 794 753 598 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2594 4906 809
Size: 507 1278 2312 1802
Fragments arranged by size:
2312 1802 1278 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 3346 4235
Size: 54 1425 50 275 18 402 1114 889
Cuts at: 4235 5864 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1114 889 402 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3829 1353
Size: 2476 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2300 2539 2661 2866 2980 3962
Size: 393 398 270 239 122 205 114 982
Cuts at: 3962 4559 5346 5614 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 982 787 597 398 393 270 268
239 205 122 114
EcoO109I rG'GnC_Cy
Cuts at: 2175 2175
Size: 5899
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
EcoRV GAT'ATC
Cuts at: 2535 3112 2535
Size: 577 5322
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 5046 1678
Size: 3368 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2950 2982 4540 4572 4614
Size: 32 135 32 1378 32 1558 32 42
Cuts at: 4614 4646 1373
Size: 32 2626
Fragments arranged by size:
2626 1558 1378 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2869 3243 3489 669
Size: 988 281 931 374 246 3079
Fragments arranged by size:
3079 988 931 374 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2272 5013 5197 1305
Size: 967 2741 184 2007
Fragments arranged by size:
2741 2007 967 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 3146 3857 4216 4375 518
Size: 648 1635 345 711 359 159 2042
Fragments arranged by size:
2042 1635 711 648 359 345 159
MspA1I CmG'CkG
Cuts at: 1606 3315 3992 5140 5385 1606
Size: 1709 677 1148 245 2120
Fragments arranged by size:
2120 1709 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4408
Fragments arranged by size:
4408 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NsiI A_TGCA'T
Cuts at: 2338 2338
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 4684
PfoI T'CCnGG_A
Cuts at: 2555 2555
Size: 5899
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2175 2175
Size: 5899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2254 2286 2254
Size: 32 5867
PstI C_TGCA'G
Cuts at: 830 3251 830
Size: 2421 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SexAI A'CCwGG_T
Cuts at: 2076 2193 2076
Size: 117 5782
SfcI C'TryA_G
Cuts at: 826 1045 2848 3046 3247 4382 5063 5254 826
Size: 219 1803 198 201 1135 681 191 1471
Fragments arranged by size:
1803 1471 1135 681 219 201 198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4003 4904 5166 820
Size: 197 926 2060 901 262 1553
Fragments arranged by size:
2060 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 2506 3823 5
Size: 47 2454 1317 2081
Fragments arranged by size:
2454 2081 1317 47
StuI AGG'CCT
Cuts at: 1517 2241 1517
Size: 724 5175
StyI C'CwwG_G
Cuts at: 513 1065 2004 3338 513
Size: 552 939 1334 3074
Fragments arranged by size:
3074 1334 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3926 3943 4096 4281 4726 325
Size: 1255 2346 17 153 185 445 1498
Fragments arranged by size:
2346 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2098 4145 5857
Size: 274 80 33 51 1443 121 2047 1712
Cuts at: 5857 96
Size: 138
Fragments arranged by size:
2047 1712 1443 274 138 121 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 4028 4028
Size: 5899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI