Restriction map for
pFN21AE9567
(Circular) (Six-base) MAPSORT of: pFN21AE9567.seq Check: 4422 from: 1 to: 6169
pFN21AE9567 6169 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2293 2560 3179 4232 5616 5884 1632
Size: 661 267 619 1053 1384 268 1917
Fragments arranged by size:
1917 1384 1053 661 619 268 267
AfeI AGC'GCT
Cuts at: 2354 3077 2354
Size: 723 5446
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 2562 5068 6045 1203
Size: 1359 2506 977 1327
Fragments arranged by size:
2506 1359 1327 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 2534 1166
Size: 1368 4801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3260 1184
Size: 2076 4093
AlwNI CAG_nnn'CTG
Cuts at: 2275 3912 5484 2275
Size: 1637 1572 2960
Fragments arranged by size:
2960 1637 1572
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 3241 3478 3704 3856 5965 1493
Size: 1748 237 226 152 2109 1697
Fragments arranged by size:
2109 1748 1697 237 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2261 2718 3150 3494 1870
Size: 73 72 246 457 432 344 4545
Fragments arranged by size:
4545 457 432 344 246 73 72
BamHI G'GATC_C
Cuts at: 3499 3866 3499
Size: 367 5802
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 2769 2798 3490 4945
Size: 325 217 627 888 94 29 692 1455
Cuts at: 4945 618
Size: 1842
Fragments arranged by size:
1842 1455 888 692 627 325 217 94
29
BanII G_rGCy'C
Cuts at: 1391 2045 2225 3416 3488 1391
Size: 654 180 1191 72 4072
Fragments arranged by size:
4072 1191 654 180 72
BbeI G_GCGC'C
Cuts at: 2773 2802 2773
Size: 29 6140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3288 928
Size: 971 1389 3809
Fragments arranged by size:
3809 1389 971
BbvCI CC'TCA_GC
Cuts at: 2324 2756 2324
Size: 432 5737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2319 3057 3234
Size: 107 122 71 631 605 647 738 177
Cuts at: 3234 4780 136
Size: 1546 1525
Fragments arranged by size:
1546 1525 738 647 631 605 177 122
107 71
BglII A'GATC_T
Cuts at: 6164 6164
Size: 6169
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2678 4230 5386 1790
Size: 420 468 1552 1156 2573
Fragments arranged by size:
2573 1552 1156 468 420
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2057 3429 3900
Size: 416 53 83 399 24 640 1372 471
Cuts at: 3900 4858 442
Size: 958 1753
Fragments arranged by size:
1753 1372 958 640 471 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4829 1239
Size: 3590 2579
Bpu10I CC'TnA_GC
Cuts at: 2324 2756 2324
Size: 432 5737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 4294 5159 5457 2059
Size: 2235 865 298 2771
Fragments arranged by size:
2771 2235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2480 3311 4832 882
Size: 617 981 831 1521 2219
Fragments arranged by size:
2219 1521 981 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 2241 2395 3960 493
Size: 711 1037 154 1565 2702
Fragments arranged by size:
2702 1565 1037 711 154
BsaBI GATnn'nnATC
Cuts at: 2087 2816 2990 6163 2087
Size: 729 174 3173 2093
Fragments arranged by size:
3173 2093 729 174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2770 2799 3976 4358
Size: 53 83 186 936 1237 29 1177 382
Cuts at: 4358 4993 275
Size: 635 1451
Fragments arranged by size:
1451 1237 1177 936 635 382 186 83
53 29
BsaWI w'CCGG_w
Cuts at: 3080 4602 5000 5274 5421 5832 3080
Size: 1522 398 274 147 411 3417
Fragments arranged by size:
3417 1522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3122 3152 1178
Size: 30 165 30 1719 30 4195
Fragments arranged by size:
4195 1719 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6169
BseYI C'CCAG_C
Cuts at: 2671 2740 3392 5372 5868 2671
Size: 69 652 1980 496 2972
Fragments arranged by size:
2972 1980 652 496 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5596
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2050 2697 2703 3619 4380 5408
Size: 275 186 49 647 6 916 761 1028
Cuts at: 5408 1540
Size: 2301
Fragments arranged by size:
2301 1028 916 761 647 275 186 49
6
BsiHKAI G_wGCw'C
Cuts at: 2451 2850 3015 3416 4230 4315 5386 2451
Size: 399 165 401 814 85 1071 3234
Fragments arranged by size:
3234 1071 814 401 399 165 85
BsmI GAATG_Cn'
Cuts at: 3680 3773 3680
Size: 93 6076
BsmBI CGTCTCn'nnnn_
Cuts at: 2150 2150
Size: 6169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2210 2225 2451 2678 2850 3015
Size: 399 255 165 15 226 227 172 165
Cuts at: 3015 3416 3488 4230 4315 5386 1391
Size: 401 72 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 401 399 255 227 226
172 165 165 85 72 15
BspEI T'CCGG_A
Cuts at: 3080 5832 3080
Size: 2752 3417
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3524 844
Size: 2680 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2866 3384 4056 1394
Size: 429 1043 518 672 3507
Fragments arranged by size:
3507 1043 672 518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 4664 4838 65
Size: 1183 1578 1838 174 1396
Fragments arranged by size:
1838 1578 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 4813 5000 1700
Size: 110 239 2764 187 2869
Fragments arranged by size:
2869 2764 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 2457 2628 1925
Size: 532 171 5466
Fragments arranged by size:
5466 532 171
BssSI C'ACGA_G
Cuts at: 2867 4229 5241 2867
Size: 1362 1012 3795
Fragments arranged by size:
3795 1362 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2334 2435 794
Size: 1540 101 4528
Fragments arranged by size:
4528 1540 101
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 2227 1184
Size: 1043 5126
BstXI CCAn_nnnn'nTGG
Cuts at: 2508 2839 2508
Size: 331 5838
BstYI r'GATC_y
Cuts at: 1972 2805 3499 3866 4251 4268 5720 5806 5818
Size: 833 694 367 385 17 1452 86 12
Cuts at: 5818 6164 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 833 694 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3405 3405
Size: 6169
BtgI C'CryG_G
Cuts at: 513 1065 2697 513
Size: 552 1632 3985
Fragments arranged by size:
3985 1632 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2218 2776 487
Size: 683 56 992 558 3880
Fragments arranged by size:
3880 992 683 558 56
BtsI GCAGTG_nn'
Cuts at: 3088 3203 3685 4479 4499 3088
Size: 115 482 794 20 4758
Fragments arranged by size:
4758 794 482 115 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DraIII CAC_nnn'GTG
Cuts at: 2395 2511 2395
Size: 116 6053
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2361 2694
Size: 54 1425 50 275 18 285 246 333
Cuts at: 2694 2700 3616 4505 6134 8
Size: 6 916 889 1629 43
Fragments arranged by size:
1629 1425 916 889 333 285 275 246
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2694 2700 3616 1537
Size: 275 882 6 916 4090
Fragments arranged by size:
4090 916 882 275 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2309 3050 3334 4099 1353
Size: 956 741 284 765 3423
Fragments arranged by size:
3423 956 765 741 284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2020 2774 4756 5130 5276 387
Size: 1064 138 431 754 1982 374 146 1280
Fragments arranged by size:
1982 1280 1064 754 431 374 146 138
EcoICRI GAG'CTC
Cuts at: 3414 3414
Size: 6169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2293 2560 3179 4232 4829 5616 5884
Size: 393 661 267 619 1053 597 787 268
Cuts at: 5884 1239
Size: 1524
Fragments arranged by size:
1524 1053 787 661 619 597 393 268
267
EcoNI CCTnn'n_nnAGG
Cuts at: 3275 3295 3275
Size: 20 6149
EcoO109I rG'GnC_Cy
Cuts at: 3063 3063
Size: 6169
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3129 3129
Size: 6169
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 2356 2773 2802 3079 5316 1678
Size: 678 417 29 277 2237 2531
Fragments arranged by size:
2531 2237 678 417 277 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2868 2900 3275 3307 4810
Size: 32 135 32 1296 32 375 32 1503
Cuts at: 4810 4842 4884 4916 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1503 1296 375 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2887 3513 3759 669
Size: 988 281 949 626 246 3079
Fragments arranged by size:
3079 988 949 626 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KasI G'GCGC_C
Cuts at: 2769 2798 2769
Size: 29 6140
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 5283 5467 1305
Size: 1161 2817 184 2007
Fragments arranged by size:
2817 2007 1161 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2363 10
Size: 54 1425 343 285 246 3816
Fragments arranged by size:
3816 1425 343 285 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2243 2534 4127 4486 4645 518
Size: 648 957 120 291 1593 359 159 2042
Fragments arranged by size:
2042 1593 957 648 359 291 159 120
MspA1I CmG'CkG
Cuts at: 1606 2275 2699 2732 3585 4262 5410 5655 1606
Size: 669 424 33 853 677 1148 245 2120
Fragments arranged by size:
2120 1148 853 677 669 424 245 33
NarI GG'CG_CC
Cuts at: 2770 2799 2770
Size: 29 6140
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5617
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 2694 2700 3616 2694
Size: 6 916 5247
Fragments arranged by size:
5247 916 6
NspI r_CATG'y
Cuts at: 1132 1229 2566 2644 3527 5072 1132
Size: 97 1337 78 883 1545 2229
Fragments arranged by size:
2229 1545 1337 883 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 2562 5068 2562
Size: 2506 3663
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 1108
Size: 1287 4882
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3063 3063
Size: 6169
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PstI C_TGCA'G
Cuts at: 830 2496 3521 830
Size: 1666 1025 3478
Fragments arranged by size:
3478 1666 1025
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
SacI G_AGCT'C
Cuts at: 3416 3416
Size: 6169
SacII CC_GC'GG
Cuts at: 2700 2700
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SanDI GG'GwC_CC
Cuts at: 3063 3063
Size: 6169
SapI GCTCTTCn'nnn_
Cuts at: 2309 2309
Size: 6169
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SexAI A'CCwGG_T
Cuts at: 2109 2507 2109
Size: 398 5771
SfcI C'TryA_G
Cuts at: 826 1045 2383 2492 3517 4652 5333 5524 826
Size: 219 1338 109 1025 1135 681 191 1471
Fragments arranged by size:
1471 1338 1135 1025 681 219 191 109
SfoI GGC'GCC
Cuts at: 2771 2800 2771
Size: 29 6140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 2017 2263 2720 3152 3496 1872
Size: 145 246 457 432 344 4545
Fragments arranged by size:
4545 457 432 344 246 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 4273 5174 5436 820
Size: 197 926 95 2235 901 262 1553
Fragments arranged by size:
2235 1553 926 901 262 197 95
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 2644 3527 1132
Size: 1512 883 3774
Fragments arranged by size:
3774 1512 883
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6169
StyI C'CwwG_G
Cuts at: 513 1065 3229 3608 513
Size: 552 2164 379 3074
Fragments arranged by size:
3074 2164 552 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4196 4213 4366 4551 4996 325
Size: 1255 2616 17 153 185 445 1498
Fragments arranged by size:
2616 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 3173 4415
Size: 274 80 33 51 1443 306 890 1242
Cuts at: 4415 6127 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1242 890 306 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3122 3154 6129 6161 3122
Size: 32 2975 32 3130
Fragments arranged by size:
3130 2975 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2839 2866 2839
Size: 27 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 2015 2261 2718 3150 3494 1870
Size: 145 246 457 432 344 4545
Fragments arranged by size:
4545 457 432 344 246 145
XmnI GAAnn'nnTTC
Cuts at: 2956 4298 2956
Size: 1342 4827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SfiI SrfI SwaI