Restriction map for
pFN21AE9565
(Circular) (Six-base) MAPSORT of: pFN21AE9565.seq Check: 14 from: 1 to: 5884
pFN21AE9565 5884 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2098 3947 5331 5599 1632
Size: 466 1849 1384 268 1917
Fragments arranged by size:
1917 1849 1384 466 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 2918 1166
Size: 1752 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2846 1184
Size: 1662 4222
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 5852
AlwNI CAG_nnn'CTG
Cuts at: 2543 3627 5199 2543
Size: 1084 1572 3228
Fragments arranged by size:
3228 1572 1084
ApaI G_GGCC'C
Cuts at: 2735 2735
Size: 5884
ApaLI G'TGCA_C
Cuts at: 2413 3941 5097 2413
Size: 1528 1156 3200
Fragments arranged by size:
3200 1528 1156
ApoI r'AATT_y
Cuts at: 1493 2723 3193 3419 3571 5680 1493
Size: 1230 470 226 152 2109 1697
Fragments arranged by size:
2109 1697 1230 470 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2772 3209 1870
Size: 73 829 437 4545
Fragments arranged by size:
4545 829 437 73
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 3205 4660 618
Size: 325 217 627 1109 309 1455 1842
Fragments arranged by size:
1842 1455 1109 627 325 309 217
BanII G_rGCy'C
Cuts at: 1391 2089 2735 2781 3203 1391
Size: 698 646 46 422 4072
Fragments arranged by size:
4072 698 646 422 46
BbeI G_GCGC'C
Cuts at: 2900 2900
Size: 5884
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2852 928
Size: 971 953 3960
Fragments arranged by size:
3960 971 953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2707 2741 4075 4109 1903
Size: 34 770 34 1334 34 3678
Fragments arranged by size:
3678 1334 770 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 3012 1548
Size: 1464 4420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2315 2471 4495
Size: 107 122 71 631 605 643 156 2024
Cuts at: 4495 136
Size: 1525
Fragments arranged by size:
2024 1525 643 631 605 156 122 107
71
BglII A'GATC_T
Cuts at: 2646 2661 5879 2646
Size: 15 3218 2651
Fragments arranged by size:
3218 2651 15
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2417 2735 3945 5101 1790
Size: 627 318 1210 1156 2573
Fragments arranged by size:
2573 1210 1156 627 318
BmgBI CAC'GTC
Cuts at: 1318 2407 1318
Size: 1089 4795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3615 4573 442
Size: 416 53 83 399 24 2198 958 1753
Fragments arranged by size:
2198 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4544 1239
Size: 3305 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3116 4009 4874 5172 3116
Size: 893 865 298 3828
Fragments arranged by size:
3828 893 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2509 4547 882
Size: 617 504 506 2038 2219
Fragments arranged by size:
2219 2038 617 506 504
BsaAI yAC'GTr
Cuts at: 493 1204 2884 3675 493
Size: 711 1680 791 2702
Fragments arranged by size:
2702 1680 791 711
BsaBI GATnn'nnATC
Cuts at: 2585 5878 2585
Size: 3293 2591
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2897 3691 4073 4708
Size: 53 83 186 936 1364 794 382 635
Cuts at: 4708 275
Size: 1451
Fragments arranged by size:
1451 1364 936 794 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4317 4715 4989 5136 5547 4317
Size: 398 274 147 411 4654
Fragments arranged by size:
4654 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2768 2798 1178
Size: 30 165 30 1365 30 4264
Fragments arranged by size:
4264 1365 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5884
BseYI C'CCAG_C
Cuts at: 2892 5087 5583 2892
Size: 2195 496 3193
Fragments arranged by size:
3193 2195 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3138 1163
Size: 573 1402 3909
Fragments arranged by size:
3909 1402 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2400 2417 2781 2862 3945 4030 5101 2073
Size: 327 17 364 81 1083 85 1071 2856
Fragments arranged by size:
2856 1083 1071 364 327 85 81 17
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
BsmBI CGTCTCn'nnnn_
Cuts at: 2606 2606
Size: 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2089 2400 2417 2735 2781 2862
Size: 399 283 16 311 17 318 46 81
Cuts at: 2862 3203 3945 4030 5101 1391
Size: 341 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 318 311 283
85 81 46 17 16
BspEI T'CCGG_A
Cuts at: 5547 5547
Size: 5884
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 2439 3239 844
Size: 1463 132 800 3489
Fragments arranged by size:
3489 1463 800 132
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4379 4553 65
Size: 1183 3131 174 1396
Fragments arranged by size:
3131 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4528 4715 1700
Size: 110 2718 187 2869
Fragments arranged by size:
2869 2718 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5884
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 2740 1184
Size: 1556 4328
BstYI r'GATC_y
Cuts at: 1972 2646 2661 2766 2817 3214 3581 3966 3983
Size: 674 15 105 51 397 367 385 17
Cuts at: 3983 5435 5521 5533 5879 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 674 397 385 367 346 105
86 51 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2519 2736 513
Size: 552 939 12 503 217 3661
Fragments arranged by size:
3661 939 552 503 217 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2290 2461 2977 487
Size: 683 56 1064 171 516 3394
Fragments arranged by size:
3394 1064 683 516 171 56
BtsI GCAGTG_nn'
Cuts at: 2967 3400 4194 4214 2967
Size: 433 794 20 4637
Fragments arranged by size:
4637 794 433 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DraIII CAC_nnn'GTG
Cuts at: 3024 3024
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2337 2401
Size: 54 1425 50 275 18 486 21 64
Cuts at: 2401 2472 2656 3331 4220 5849 8
Size: 71 184 675 889 1629 43
Fragments arranged by size:
1629 1425 889 675 486 275 184 71
64 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3153 3814 1353
Size: 1800 661 3423
Fragments arranged by size:
3423 1800 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2584 4471 4845 4991 387
Size: 1064 138 995 1887 374 146 1280
Fragments arranged by size:
1887 1280 1064 995 374 146 138
EcoICRI GAG'CTC
Cuts at: 2779 2779
Size: 5884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 3947 4544 5331 5599 1239
Size: 393 466 1849 597 787 268 1524
Fragments arranged by size:
1849 1524 787 597 466 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2732 2732
Size: 5884
EcoRI G'AATT_C
Cuts at: 2723 3193 3571 2723
Size: 470 378 5036
Fragments arranged by size:
5036 470 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 5884
FspI TGC'GCA
Cuts at: 2285 2435 4390 2285
Size: 150 1955 3779
Fragments arranged by size:
3779 1955 150
HaeII r_GCGC'y
Cuts at: 1678 2900 5031 1678
Size: 1222 2131 2531
Fragments arranged by size:
2531 2131 1222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3004 3036 4525 4557 4599
Size: 32 135 32 1432 32 1489 32 42
Cuts at: 4599 4631 1373
Size: 32 2626
Fragments arranged by size:
2626 1489 1432 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 3228 3474 669
Size: 988 281 487 803 246 3079
Fragments arranged by size:
3079 988 803 487 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KasI G'GCGC_C
Cuts at: 2896 2896
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3138 3483 3138
Size: 345 5539
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 2339 2474 10
Size: 54 1425 343 486 21 135 3420
Fragments arranged by size:
3420 1425 486 343 135 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2918 2996 3842 4201 4360 518
Size: 648 1035 717 78 846 359 159 2042
Fragments arranged by size:
2042 1035 846 717 648 359 159 78
MspA1I CmG'CkG
Cuts at: 1606 2512 3300 3977 5125 5370 1606
Size: 906 788 677 1148 245 2120
Fragments arranged by size:
2120 1148 906 788 677 245
NarI GG'CG_CC
Cuts at: 2897 2897
Size: 5884
NcoI C'CATG_G
Cuts at: 513 1065 2004 2016 513
Size: 552 939 12 4381
Fragments arranged by size:
4381 939 552 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 3242 4787 1132
Size: 97 2013 1545 2229
Fragments arranged by size:
2229 2013 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5884
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5884
PfoI T'CCnGG_A
Cuts at: 2168 2168
Size: 5884
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 3953 3985 5466 5498 2768
Size: 32 1153 32 1481 32 3154
Fragments arranged by size:
3154 1481 1153 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PspOMI G'GGCC_C
Cuts at: 2731 2731
Size: 5884
PstI C_TGCA'G
Cuts at: 830 3236 830
Size: 2406 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
SacI G_AGCT'C
Cuts at: 2781 2781
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 3059 4132 5844 3059
Size: 1073 1712 3099
Fragments arranged by size:
3099 1712 1073
SfcI C'TryA_G
Cuts at: 826 1045 2119 3232 4367 5048 5239 826
Size: 219 1074 1113 1135 681 191 1471
Fragments arranged by size:
1471 1135 1113 1074 681 219 191
SfoI GGC'GCC
Cuts at: 2898 2898
Size: 5884
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 2774 3211 1872
Size: 902 437 4545
Fragments arranged by size:
4545 902 437
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3131 3988 4889 5151 820
Size: 197 926 1188 857 901 262 1553
Fragments arranged by size:
1553 1188 926 901 857 262 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5884
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2556 3323 513
Size: 552 939 12 540 767 3074
Fragments arranged by size:
3074 939 767 552 540 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 3928 4081 4266 4711 325
Size: 1255 2331 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3057 4130 5842
Size: 274 80 33 51 1443 1080 1073 1712
Cuts at: 5842 96
Size: 138
Fragments arranged by size:
1712 1443 1080 1073 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5844 5876 5844
Size: 32 5852
Tth111I GACn'n_nGTC
Cuts at: 1316 2569 3050 1316
Size: 1253 481 4150
Fragments arranged by size:
4150 1253 481
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2023 2467 2540 3121 3160 2023
Size: 444 73 581 39 4747
Fragments arranged by size:
4747 581 444 73 39
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 2772 3209 1870
Size: 902 437 4545
Fragments arranged by size:
4545 902 437
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI