Restriction map for
pFN21AE9564
(Circular) (Six-base) MAPSORT of: pFN21AE9564.seq Check: 4956 from: 1 to: 5911
pFN21AE9564 5911 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3254 1937
Size: 1317 4594
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3974 5358 5626 1632
Size: 2342 1384 268 1917
Fragments arranged by size:
2342 1917 1384 268
AfeI AGC'GCT
Cuts at: 2408 2408
Size: 5911
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 2079 4810 5787 1203
Size: 876 2731 977 1327
Fragments arranged by size:
2731 1327 977 876
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 2608 3040 3654 5226 2608
Size: 432 614 1572 3293
Fragments arranged by size:
3293 1572 614 432
ApaI G_GGCC'C
Cuts at: 2022 2022
Size: 5911
ApaLI G'TGCA_C
Cuts at: 3968 5124 3968
Size: 1156 4755
ApoI r'AATT_y
Cuts at: 1493 3220 3446 3598 5707 1493
Size: 1727 226 152 2109 1697
Fragments arranged by size:
2109 1727 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2275 2554 2587 3106 3236 1870
Size: 73 332 279 33 519 130 4545
Fragments arranged by size:
4545 519 332 279 130 73 33
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2141 2598 3232 4687
Size: 325 217 627 275 79 457 634 1455
Cuts at: 4687 618
Size: 1842
Fragments arranged by size:
1842 1455 634 627 457 325 275 217
79
BanII G_rGCy'C
Cuts at: 1391 2012 2022 2869 2939 2981 3230 1391
Size: 621 10 847 70 42 249 4072
Fragments arranged by size:
4072 847 621 249 70 42 10
BbeI G_GCGC'C
Cuts at: 2066 2145 2602 2066
Size: 79 457 5375
Fragments arranged by size:
5375 457 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2350 928
Size: 971 451 4489
Fragments arranged by size:
4489 971 451
BbvCI CC'TCA_GC
Cuts at: 3200 3200
Size: 5911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 2327 2486
Size: 107 122 71 631 605 368 287 159
Cuts at: 2486 2495 3164 4522 136
Size: 9 669 1358 1525
Fragments arranged by size:
1525 1358 669 631 605 368 287 159
122 107 71 9
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 2675 3328 2675
Size: 653 5258
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2375 2561 3972 5128 1790
Size: 232 353 186 1411 1156 2573
Fragments arranged by size:
2573 1411 1156 353 232 186
BmgBI CAC'GTC
Cuts at: 1318 2082 2881 1318
Size: 764 799 4348
Fragments arranged by size:
4348 799 764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3642 4600 442
Size: 416 53 83 399 24 2225 958 1753
Fragments arranged by size:
2225 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3187 3187
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2953 2995 4571 1239
Size: 1714 42 1576 2579
Fragments arranged by size:
2579 1714 1576 42
Bpu10I CC'TnA_GC
Cuts at: 2030 3200 2030
Size: 1170 4741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4901 5199 4036
Size: 865 298 4748
Fragments arranged by size:
4748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2455 4574 882
Size: 617 956 2119 2219
Fragments arranged by size:
2219 2119 956 617
BsaAI yAC'GTr
Cuts at: 493 1204 2269 2562 3702 493
Size: 711 1065 293 1140 2702
Fragments arranged by size:
2702 1140 1065 711 293
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 2142 2200 2418
Size: 53 83 186 936 530 79 58 218
Cuts at: 2418 2599 2710 2755 3718 4100 4735 275
Size: 181 111 45 963 382 635 1451
Fragments arranged by size:
1451 963 936 635 530 382 218 186
181 111 83 79 58 53 45
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5686
Fragments arranged by size:
5686 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2639 1186
Size: 1453 4458
BseYI C'CCAG_C
Cuts at: 2137 2383 2760 3139 5114 5610 2137
Size: 246 377 379 1975 496 2438
Fragments arranged by size:
2438 1975 496 379 377 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 2214 2322 2429 2457 3361
Size: 275 186 87 126 108 107 28 904
Cuts at: 3361 4122 5150 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186 126 108
107 87 28
BsiHKAI G_wGCw'C
Cuts at: 2869 2981 3972 4057 5128 2869
Size: 112 991 85 1071 3652
Fragments arranged by size:
3652 1071 991 112 85
BsmI GAATG_Cn'
Cuts at: 3422 3515 3422
Size: 93 5818
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2022 2375 2561 2869 2939 2981
Size: 399 222 10 353 186 308 70 42
Cuts at: 2981 3230 3972 4057 5128 1391
Size: 249 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 353 308 249 222
186 85 70 42 10
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 3266 844
Size: 1319 1103 3489
Fragments arranged by size:
3489 1319 1103
BsrBI CCG'CTC
Cuts at: 1394 1823 2208 2715 2781 3798 1394
Size: 429 385 507 66 1017 3507
Fragments arranged by size:
3507 1017 507 429 385 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2321 2751 4555 4742 1700
Size: 110 511 430 1804 187 2869
Fragments arranged by size:
2869 1804 511 430 187 110
BsrGI T'GTAC_A
Cuts at: 96 2298 3054 96
Size: 2202 756 2953
Fragments arranged by size:
2953 2202 756
BssHII G'CGCG_C
Cuts at: 1925 2280 2311 2463 2465 2525 1925
Size: 355 31 152 2 60 5311
Fragments arranged by size:
5311 355 152 60 31 2
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2565 2612 794
Size: 1771 47 4093
Fragments arranged by size:
4093 1771 47
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2158 2245 2257 2137
Size: 21 87 12 5791
Fragments arranged by size:
5791 87 21 12
BstYI r'GATC_y
Cuts at: 1972 3036 3241 3608 3993 4010 5462 5548 5560
Size: 1064 205 367 385 17 1452 86 12
Cuts at: 5560 5906 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1064 385 367 346 205 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2737 2737
Size: 5911
BtgI C'CryG_G
Cuts at: 513 1065 2184 2400 2481 2490 2505 3132 513
Size: 552 1119 216 81 9 15 627 3292
Fragments arranged by size:
3292 1119 627 552 216 81 15 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2794 487
Size: 683 56 1568 3604
Fragments arranged by size:
3604 1568 683 56
BtsI GCAGTG_nn'
Cuts at: 2301 2606 3427 4221 4241 2301
Size: 305 821 794 20 3971
Fragments arranged by size:
3971 821 794 305 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2812 2847 529
Size: 35 2248 35 3593
Fragments arranged by size:
3593 2248 35 35
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DraIII CAC_nnn'GTG
Cuts at: 2565 2881 2565
Size: 316 5595
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2211 2235
Size: 54 1425 50 275 18 255 126 24
Cuts at: 2235 2247 2319 2397 2426 2478 2487 2502 2961
Size: 12 72 78 29 52 9 15 459
Cuts at: 2961 2996 3009 3358 4247 5876 8
Size: 35 13 349 889 1629 43
Fragments arranged by size:
1629 1425 889 459 349 275 255 126
78 72 54 52 50 43 35 29
24 18 15 13 12 9
EagI C'GGCC_G
Cuts at: 1537 1812 2085 2211 2319 2426 3358 1537
Size: 275 273 126 108 107 932 4090
Fragments arranged by size:
4090 932 275 273 126 108 107
EarI CTCTTCn'nnn_
Cuts at: 1353 2627 3841 1353
Size: 1274 1214 3423
Fragments arranged by size:
3423 1274 1214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2779 2788 4498 4872 5018 387
Size: 1064 138 1190 9 1710 374 146 1280
Fragments arranged by size:
1710 1280 1190 1064 374 146 138 9
EcoICRI GAG'CTC
Cuts at: 2867 2979 2867
Size: 112 5799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2953 2995 3974 4571 5358 5626 1239
Size: 393 1321 42 979 597 787 268 1524
Fragments arranged by size:
1524 1321 979 787 597 393 268 42
EcoO109I rG'GnC_Cy
Cuts at: 2019 2071 2328 2582 2741 2019
Size: 52 257 254 159 5189
Fragments arranged by size:
5189 257 254 159 52
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FspI TGC'GCA
Cuts at: 3179 4417 3179
Size: 1238 4673
HaeII r_GCGC'y
Cuts at: 1678 2066 2106 2145 2165 2171 2410 2477 2513
Size: 388 40 39 20 6 239 67 36
Cuts at: 2513 2602 2960 3159 5058 1678
Size: 89 358 199 1899 2531
Fragments arranged by size:
2531 1899 388 358 239 199 89 67
40 39 36 20 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373
Size: 32 135 32 2980 32 42 32 2626
Fragments arranged by size:
2980 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KasI G'GCGC_C
Cuts at: 2062 2141 2598 2062
Size: 79 457 5375
Fragments arranged by size:
5375 457 79
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2175 5025 5209 1305
Size: 870 2850 184 2007
Fragments arranged by size:
2850 2007 870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 2249 10
Size: 54 1425 343 405 12 3672
Fragments arranged by size:
3672 1425 405 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 2915 3869 4228 4387 518
Size: 648 1233 516 954 359 159 2042
Fragments arranged by size:
2042 1233 954 648 516 359 159
MspA1I CmG'CkG
Cuts at: 1606 2186 2377 2431 2483 2492 2521 2679 2703
Size: 580 191 54 52 9 29 158 24
Cuts at: 2703 2850 2995 3134 3195 3327 4004 5152 5397
Size: 147 145 139 61 132 677 1148 245
Cuts at: 5397 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 580 245 191 158 147
145 139 132 61 54 52 29 24
9
NaeI GCC'GGC
Cuts at: 2323 2753 2323
Size: 430 5481
NarI GG'CG_CC
Cuts at: 2063 2142 2599 2063
Size: 79 457 5375
Fragments arranged by size:
5375 457 79
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5359
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NgoMIV G'CCGG_C
Cuts at: 2321 2751 2321
Size: 430 5481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 2426 3358 2426
Size: 932 4979
NspI r_CATG'y
Cuts at: 1132 1229 2058 3269 4814 1132
Size: 97 829 1211 1545 2229
Fragments arranged by size:
2229 1545 1211 829 97
PasI CC'CwG_GG
Cuts at: 1348 2067 1348
Size: 719 5192
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2341 2881 3040 1108
Size: 1233 540 159 3979
Fragments arranged by size:
3979 1233 540 159
PfoI T'CCnGG_A
Cuts at: 3026 3039 3026
Size: 13 5898
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PmlI CAC'GTG
Cuts at: 2269 2562 2269
Size: 293 5618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2582 2071
Size: 511 5400
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PspOMI G'GGCC_C
Cuts at: 2018 2018
Size: 5911
PstI C_TGCA'G
Cuts at: 830 2550 2994 3263 830
Size: 1720 444 269 3478
Fragments arranged by size:
3478 1720 444 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
SacI G_AGCT'C
Cuts at: 2869 2981 2869
Size: 112 5799
SacII CC_GC'GG
Cuts at: 2187 2484 2493 3135 2187
Size: 297 9 642 4963
Fragments arranged by size:
4963 642 297 9
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SanDI GG'GwC_CC
Cuts at: 2071 2582 2071
Size: 511 5400
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 2546 2990 3259 4394 5075 5266 826
Size: 219 1501 444 269 1135 681 191 1471
Fragments arranged by size:
1501 1471 1135 681 444 269 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2327 2486 2495 2327
Size: 159 9 5743
Fragments arranged by size:
5743 159 9
SfoI GGC'GCC
Cuts at: 2064 2143 2600 2064
Size: 79 457 5375
Fragments arranged by size:
5375 457 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 2277 2556 2589 3238 1872
Size: 405 279 33 649 4545
Fragments arranged by size:
4545 649 405 279 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4015 4916 5178 820
Size: 197 926 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5911
StyI C'CwwG_G
Cuts at: 513 1065 2499 3047 3120 3350 513
Size: 552 1434 548 73 230 3074
Fragments arranged by size:
3074 1434 552 548 230 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2443 3040 3938 3955 4108 4293 4738
Size: 1255 863 597 898 17 153 185 445
Cuts at: 4738 325
Size: 1498
Fragments arranged by size:
1498 1255 898 863 597 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 3054 4157
Size: 274 80 33 51 1443 321 756 1103
Cuts at: 4157 5869 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1103 756 321 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2635 2635
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 2275 2554 2587 3236 1870
Size: 405 279 33 649 4545
Fragments arranged by size:
4545 649 405 279 33
XmnI GAAnn'nnTTC
Cuts at: 4040 4040
Size: 5911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PspXI PsrI PvuII RsrII SapI SexAI SrfI
SwaI