Restriction map for
pFN21AE9561
(Circular) (Six-base) MAPSORT of: pFN21AE9561.seq Check: 1142 from: 1 to: 5809
pFN21AE9561 5809 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2310 2310
Size: 5809
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2874 3152 1937
Size: 937 278 4594
Fragments arranged by size:
4594 937 278
Acc65I G'GTAC_C
Cuts at: 2364 3130 2364
Size: 766 5043
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2782 3872 5256 5524 1632
Size: 1150 1090 1384 268 1917
Fragments arranged by size:
1917 1384 1150 1090 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2312 1184
Size: 1128 4681
AlwNI CAG_nnn'CTG
Cuts at: 2066 3552 5124 2066
Size: 1486 1572 2751
Fragments arranged by size:
2751 1572 1486
ApaI G_GGCC'C
Cuts at: 2281 2993 3056 2281
Size: 712 63 5034
Fragments arranged by size:
5034 712 63
ApaLI G'TGCA_C
Cuts at: 2297 3866 5022 2297
Size: 1569 1156 3084
Fragments arranged by size:
3084 1569 1156
ApoI r'AATT_y
Cuts at: 1493 3118 3344 3496 5605 1493
Size: 1625 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 226 152
AscI GG'CGCG_CC
Cuts at: 2707 2707
Size: 5809
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 2143 2986 3096 3134 1870
Size: 73 200 843 110 38 4545
Fragments arranged by size:
4545 843 200 110 73 38
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2364 2379 2467 2489 3130
Size: 325 217 627 577 15 88 22 641
Cuts at: 3130 4585 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 641 627 577 325 217 88
22 15
BanII G_rGCy'C
Cuts at: 1391 2281 2517 2993 3056 3063 3128 1391
Size: 890 236 476 63 7 65 4072
Fragments arranged by size:
4072 890 476 236 65 63 7
BbeI G_GCGC'C
Cuts at: 2471 2471
Size: 5809
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BbvCI CC'TCA_GC
Cuts at: 2477 2477
Size: 5809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4911 3701
Size: 1210 4599
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2717 2997 4420
Size: 107 122 71 631 605 1045 280 1423
Cuts at: 4420 136
Size: 1525
Fragments arranged by size:
1525 1423 1045 631 605 280 122 107
71
BglII A'GATC_T
Cuts at: 5804 5804
Size: 5809
BlpI GC'TnA_GC
Cuts at: 3226 3226
Size: 5809
Bme1580I G_kGCm'C
Cuts at: 1790 2099 2235 2281 2301 2526 2953 2993 3056
Size: 309 136 46 20 225 427 40 63
Cuts at: 3056 3870 5026 1790
Size: 814 1156 2573
Fragments arranged by size:
2573 1156 814 427 309 225 136 63
46 40 20
BmgBI CAC'GTC
Cuts at: 1318 2560 1318
Size: 1242 4567
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3540 4498 442
Size: 416 53 83 399 24 2123 958 1753
Fragments arranged by size:
2123 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 4469 1239
Size: 1292 1938 2579
Fragments arranged by size:
2579 1938 1292
Bpu10I CC'TnA_GC
Cuts at: 2477 2477
Size: 5809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3934 4799 5097 3934
Size: 865 298 4646
Fragments arranged by size:
4646 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4472 882
Size: 617 2973 2219
Fragments arranged by size:
2973 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 5803 5803
Size: 5809
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2468 2938 3012 3616
Size: 53 83 186 936 935 470 74 604
Cuts at: 3616 3998 4633 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 935 635 604 470 382 186
83 74 53
BsaWI w'CCGG_w
Cuts at: 3103 4242 4640 4914 5061 5472 3103
Size: 1139 398 274 147 411 3440
Fragments arranged by size:
3440 1139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2837 2867 1178
Size: 30 165 30 1434 30 4120
Fragments arranged by size:
4120 1434 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5809
BseYI C'CCAG_C
Cuts at: 2092 2745 5012 5508 2092
Size: 653 2267 496 2393
Fragments arranged by size:
2393 2267 653 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5236
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2673 2788 2932 3259 4020 5048
Size: 275 186 672 115 144 327 761 1028
Cuts at: 5048 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 672 327 275 186 144
115
BsiHKAI G_wGCw'C
Cuts at: 2130 2301 2517 3870 3955 5026 2130
Size: 171 216 1353 85 1071 2913
Fragments arranged by size:
2913 1353 1071 216 171 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
BsmBI CGTCTCn'nnnn_
Cuts at: 3003 3003
Size: 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2130 2235 2281 2301 2517 2526
Size: 399 309 31 105 46 20 216 9
Cuts at: 2526 2953 2993 3056 3063 3128 3870 3955 5026
Size: 427 40 63 7 65 742 85 1071
Cuts at: 5026 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 427 399 309 216 105
85 65 63 46 40 31 20 9
7
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 3698 5428 3698
Size: 1730 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 2592 3164 844
Size: 1466 282 572 3489
Fragments arranged by size:
3489 1466 572 282
BsrBI CCG'CTC
Cuts at: 1394 1823 2869 3696 1394
Size: 429 1046 827 3507
Fragments arranged by size:
3507 1046 827 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2113 4304 4478 65
Size: 1183 865 2191 174 1396
Fragments arranged by size:
2191 1396 1183 865 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2576 4453 4640 1700
Size: 110 766 1877 187 2869
Fragments arranged by size:
2869 1877 766 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 2395 2505 2540 2707 2901 2903 1925
Size: 470 110 35 167 194 2 4831
Fragments arranged by size:
4831 470 194 167 110 35 2
BssSI C'ACGA_G
Cuts at: 3869 4881 3869
Size: 1012 4797
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2386 794
Size: 1592 4217
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 3139 3506 3891 3908 5360 5446 5458 5804
Size: 1167 367 385 17 1452 86 12 346
Cuts at: 5804 1972
Size: 1977
Fragments arranged by size:
1977 1452 1167 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2172 2355 2592 2646 2655
Size: 552 939 51 117 183 237 54 9
Cuts at: 2655 3079 513
Size: 424 3243
Fragments arranged by size:
3243 939 552 424 237 183 117 54
51 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2130 2436 2833 3325 4119 4139 2067
Size: 63 306 397 492 794 20 3737
Fragments arranged by size:
3737 794 492 397 306 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 3113 3113
Size: 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2562 2670
Size: 54 1425 50 275 18 540 192 108
Cuts at: 2670 2724 2785 2805 2921 2929 3046 3256 4145
Size: 54 61 20 116 8 117 210 889
Cuts at: 4145 5774 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 540 275 210 192 117
116 108 61 54 54 50 43 20
18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2670 2785 2929 3256 1537
Size: 275 858 115 144 327 4090
Fragments arranged by size:
4090 858 327 275 144 115
EarI CTCTTCn'nnn_
Cuts at: 1353 2465 3739 1353
Size: 1112 1274 3423
Fragments arranged by size:
3423 1274 1112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2698 2900 2963 3099 4396 4770
Size: 1064 138 1109 202 63 136 1297 374
Cuts at: 4770 4916 387
Size: 146 1280
Fragments arranged by size:
1297 1280 1109 1064 374 202 146 138
136 63
EcoICRI GAG'CTC
Cuts at: 2515 2515
Size: 5809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2531 2782 3872 4469 5256 5524 1239
Size: 393 899 251 1090 597 787 268 1524
Fragments arranged by size:
1524 1090 899 787 597 393 268 251
EcoO109I rG'GnC_Cy
Cuts at: 2277 2278 2651 3052 3071 2277
Size: 1 373 401 19 5015
Fragments arranged by size:
5015 401 373 19 1
EcoRI G'AATT_C
Cuts at: 3118 3496 3118
Size: 378 5431
FspI TGC'GCA
Cuts at: 2405 4315 2405
Size: 1910 3899
HaeII r_GCGC'y
Cuts at: 1678 2459 2471 4956 1678
Size: 781 12 2485 2531
Fragments arranged by size:
2531 2485 781 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4450 4482 4524 4556 1373
Size: 32 135 32 2878 32 42 32 2626
Fragments arranged by size:
2878 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3153 3399 669
Size: 988 281 1215 246 3079
Fragments arranged by size:
3079 1215 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KasI G'GCGC_C
Cuts at: 2467 2467
Size: 5809
KpnI G_GTAC'C
Cuts at: 2368 3134 2368
Size: 766 5043
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4923 5107 1305
Size: 3618 184 2007
Fragments arranged by size:
3618 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 10
Size: 54 1425 343 732 3255
Fragments arranged by size:
3255 1425 732 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3767 4126 4285 518
Size: 648 2601 359 159 2042
Fragments arranged by size:
2601 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2386 2657 3225 3902 5050 5295 1606
Size: 780 271 568 677 1148 245 2120
Fragments arranged by size:
2120 1148 780 677 568 271 245
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 5809
NarI GG'CG_CC
Cuts at: 2468 2468
Size: 5809
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4318
Fragments arranged by size:
4318 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 2929 3256 2929
Size: 327 5482
NspI r_CATG'y
Cuts at: 1132 1229 3167 4712 1132
Size: 97 1938 1545 2229
Fragments arranged by size:
2229 1938 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2039 3063 1348
Size: 691 1024 4094
Fragments arranged by size:
4094 1024 691
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5809
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2651 3071 2651
Size: 420 5389
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PspOMI G'GGCC_C
Cuts at: 2277 2989 3052 2277
Size: 712 63 5034
Fragments arranged by size:
5034 712 63
PstI C_TGCA'G
Cuts at: 830 2136 3161 830
Size: 1306 1025 3478
Fragments arranged by size:
3478 1306 1025
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
SacI G_AGCT'C
Cuts at: 2517 2517
Size: 5809
SacII CC_GC'GG
Cuts at: 2658 2658
Size: 5809
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SanDI GG'GwC_CC
Cuts at: 2651 3071 2651
Size: 420 5389
SapI GCTCTTCn'nnn_
Cuts at: 2465 2465
Size: 5809
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SfcI C'TryA_G
Cuts at: 826 1045 2132 3157 4292 4973 5164 826
Size: 219 1087 1025 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 1025 681 219 191
SfoI GGC'GCC
Cuts at: 2469 2469
Size: 5809
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 3136 1872
Size: 1264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3913 4814 5076 820
Size: 197 926 1970 901 262 1553
Fragments arranged by size:
1970 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5809
StyI C'CwwG_G
Cuts at: 513 1065 2004 3248 513
Size: 552 939 1244 3074
Fragments arranged by size:
3074 1244 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3836 3853 4006 4191 4636 325
Size: 1255 2256 17 153 185 445 1498
Fragments arranged by size:
2256 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4055 5767 96
Size: 274 80 33 51 1443 2078 1712 138
Fragments arranged by size:
2078 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5769 5801 5769
Size: 32 5777
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2719 2254
Size: 465 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 3134 1870
Size: 1264 4545
XmnI GAAnn'nnTTC
Cuts at: 3938 3938
Size: 5809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SexAI SfiI SrfI SwaI