Restriction map for
pFN21AE9560
(Circular) (Six-base) MAPSORT of: pFN21AE9560.seq Check: 1392 from: 1 to: 5860
pFN21AE9560 5860 bp
With 182 enzymes: SgfI *
March 28, 2012 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2421 2750 2421
Size: 329 5531
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2079 3203 1937
Size: 142 1124 4594
Fragments arranged by size:
4594 1124 142
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3923 5307 5575 1632
Size: 2291 1384 268 1917
Fragments arranged by size:
2291 1917 1384 268
AfeI AGC'GCT
Cuts at: 2426 2426
Size: 5860
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 2481 3030 4759 5736 1203
Size: 1278 549 1729 977 1327
Fragments arranged by size:
1729 1327 1278 977 549
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 2415 1166
Size: 1249 4611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2924 1184
Size: 1740 4120
AlwNI CAG_nnn'CTG
Cuts at: 2946 3603 5175 2946
Size: 657 1572 3631
Fragments arranged by size:
3631 1572 657
ApaI G_GGCC'C
Cuts at: 2557 2557
Size: 5860
ApaLI G'TGCA_C
Cuts at: 2540 3917 5073 2540
Size: 1377 1156 3327
Fragments arranged by size:
3327 1377 1156
ApoI r'AATT_y
Cuts at: 1493 3169 3395 3547 5656 1493
Size: 1676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1676 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2932 3185 1870
Size: 73 989 253 4545
Fragments arranged by size:
4545 989 253 73
BamHI G'GATC_C
Cuts at: 3010 3190 3557 3010
Size: 180 367 5313
Fragments arranged by size:
5313 367 180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2227 2242 2320 2574
Size: 325 217 627 273 167 15 78 254
Cuts at: 2574 2963 3137 3181 4636 618
Size: 389 174 44 1455 1842
Fragments arranged by size:
1842 1455 627 389 325 273 254 217
174 167 78 44 15
BanII G_rGCy'C
Cuts at: 1391 2193 2557 2939 3069 3179 1391
Size: 802 364 382 130 110 4072
Fragments arranged by size:
4072 802 382 364 130 110
BbeI G_GCGC'C
Cuts at: 2578 2578
Size: 5860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4889
BbvCI CC'TCA_GC
Cuts at: 2849 2849
Size: 5860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2306 2340 4051 4085 1903
Size: 34 369 34 1711 34 3678
Fragments arranged by size:
3678 1711 369 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2055 2236 2318
Size: 107 122 71 631 605 383 181 82
Cuts at: 2318 2624 3104 4471 136
Size: 306 480 1367 1525
Fragments arranged by size:
1525 1367 631 605 480 383 306 181
122 107 82 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 2987 3277 2987
Size: 290 5570
Bme1580I G_kGCm'C
Cuts at: 1790 2212 2232 2247 2544 2557 2898 2976 3140
Size: 422 20 15 297 13 341 78 164
Cuts at: 3140 3921 5077 1790
Size: 781 1156 2573
Fragments arranged by size:
2573 1156 781 422 341 297 164 78
20 15 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3591 4549 442
Size: 416 53 83 399 24 2174 958 1753
Fragments arranged by size:
2174 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 3140 4520 1239
Size: 968 933 1380 2579
Fragments arranged by size:
2579 1380 968 933
Bpu10I CC'TnA_GC
Cuts at: 2849 2849
Size: 5860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2971 3031 3651 493
Size: 711 1767 60 620 2702
Fragments arranged by size:
2702 1767 711 620 60
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2575 3667 4049 4684
Size: 53 83 186 936 1042 1092 382 635
Cuts at: 4684 275
Size: 1451
Fragments arranged by size:
1451 1092 1042 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2099 4293 4691 4965 5112 5523 2099
Size: 2194 398 274 147 411 2436
Fragments arranged by size:
2436 2194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3136 1186
Size: 1950 3910
BseYI C'CCAG_C
Cuts at: 2038 2072 2223 2897 2939 2975 5063 5559 2038
Size: 34 151 674 42 36 2088 496 2339
Fragments arranged by size:
2339 2088 674 496 151 42 36 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2792 1163
Size: 573 1056 4231
Fragments arranged by size:
4231 1056 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2466 2619 2677 2830 3039 3080
Size: 275 186 465 153 58 153 209 41
Cuts at: 3080 3310 4071 5099 1540
Size: 230 761 1028 2301
Fragments arranged by size:
2301 1028 761 465 275 230 209 186
153 153 58 41
BsiHKAI G_wGCw'C
Cuts at: 2099 2193 2544 2685 2993 3921 4006 5077 2099
Size: 94 351 141 308 928 85 1071 2882
Fragments arranged by size:
2882 1071 928 351 308 141 94 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2193 2212 2232 2247 2544 2557
Size: 399 309 94 19 20 15 297 13
Cuts at: 2557 2685 2898 2939 2976 2993 3069 3140 3179
Size: 128 213 41 37 17 76 71 39
Cuts at: 3179 3921 4006 5077 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 309 297 213 128
94 85 76 71 41 39 37 20
19 17 15 13
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2421 2750 2913 3215 844
Size: 1577 329 163 302 3489
Fragments arranged by size:
3489 1577 329 302 163
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 3747 1394
Size: 429 268 1656 3507
Fragments arranged by size:
3507 1656 429 268
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2281 2571 2731 2829 4504 4691 1700
Size: 110 471 290 160 98 1675 187 2869
Fragments arranged by size:
2869 1675 471 290 187 160 110 98
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 2666 1925
Size: 741 5119
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2548 794
Size: 1754 4106
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2293 2417 2239
Size: 54 124 5682
Fragments arranged by size:
5682 124 54
BstYI r'GATC_y
Cuts at: 1972 3010 3190 3557 3942 3959 5411 5497 5509
Size: 1038 180 367 385 17 1452 86 12
Cuts at: 5509 5855 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1038 385 367 346 180 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2143 2396 2460 2880 3093 513
Size: 552 1078 253 64 420 213 3280
Fragments arranged by size:
3280 1078 552 420 253 213 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 2210 2627 2639 2868 3376 4170 4190 2210
Size: 417 12 229 508 794 20 3880
Fragments arranged by size:
3880 794 508 417 229 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2310 2419
Size: 54 1425 50 275 18 453 27 109
Cuts at: 2419 2457 2463 2505 2569 2616 2778 2820 2827
Size: 38 6 42 64 47 162 42 7
Cuts at: 2827 2839 3036 3307 4196 5825 8
Size: 12 197 271 889 1629 43
Fragments arranged by size:
1629 1425 889 453 275 271 197 162
109 64 54 50 47 43 42 42
38 27 18 12 7 6
EagI C'GGCC_G
Cuts at: 1537 1812 2463 2616 2827 3036 3307 1537
Size: 275 651 153 211 209 271 4090
Fragments arranged by size:
4090 651 275 271 211 209 153
EarI CTCTTCn'nnn_
Cuts at: 1353 3074 3790 1353
Size: 1721 716 3423
Fragments arranged by size:
3423 1721 716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2866 4447 4821 4967 387
Size: 1064 138 1277 1581 374 146 1280
Fragments arranged by size:
1581 1280 1277 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2191 2191
Size: 5860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 3140 3923 4520 5307 5575 1239
Size: 393 575 933 783 597 787 268 1524
Fragments arranged by size:
1524 933 787 783 597 575 393 268
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2300 2390 2428 2578 2753 2836 2946 2987
Size: 622 90 38 150 175 83 110 41
Cuts at: 2987 5007 1678
Size: 2020 2531
Fragments arranged by size:
2531 2020 622 175 150 110 90 83
41 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373
Size: 32 135 32 2929 32 42 32 2626
Fragments arranged by size:
2929 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KasI G'GCGC_C
Cuts at: 2574 2574
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3097 4974 5158 1305
Size: 1792 1877 184 2007
Fragments arranged by size:
2007 1877 1792 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2312 2421 2459 2780 10
Size: 54 1425 343 480 109 38 321 3090
Fragments arranged by size:
3090 1425 480 343 321 109 54 38
MslI CAynn'nnrTG
Cuts at: 518 1166 2415 2484 3818 4177 4336 518
Size: 648 1249 69 1334 359 159 2042
Fragments arranged by size:
2042 1334 1249 648 359 159 69
MspA1I CmG'CkG
Cuts at: 1606 2145 2398 2548 2647 2826 3003 3276 3953
Size: 539 253 150 99 179 177 273 677
Cuts at: 3953 5101 5346 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 539 273 253 245 179
177 150 99
NaeI GCC'GGC
Cuts at: 2283 2573 2831 2283
Size: 290 258 5312
Fragments arranged by size:
5312 290 258
NarI GG'CG_CC
Cuts at: 2575 2575
Size: 5860
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5308
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NgoMIV G'CCGG_C
Cuts at: 2281 2571 2829 2281
Size: 290 258 5312
Fragments arranged by size:
5312 290 258
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 2616 3307 2616
Size: 691 5169
NruI TCG'CGA
Cuts at: 2360 2360
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2485 3218 4763 1132
Size: 97 1256 733 1545 2229
Fragments arranged by size:
2229 1545 1256 733 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 2481 4759 2481
Size: 2278 3582
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2946 1108
Size: 1838 4022
PfoI T'CCnGG_A
Cuts at: 2193 2193
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2553 2553
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2646 3212 830
Size: 1816 566 3478
Fragments arranged by size:
3478 1816 566
PvuI CG_AT'CG
Cuts at: 2001 2677 2001
Size: 676 5184
PvuII CAG'CTG
Cuts at: 3003 3003
Size: 5860
SacI G_AGCT'C
Cuts at: 2193 2193
Size: 5860
SacII CC_GC'GG
Cuts at: 2146 2399 2146
Size: 253 5607
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SapI GCTCTTCn'nnn_
Cuts at: 3074 3074
Size: 5860
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2642 3208 4343 5024 5215 826
Size: 219 1597 566 1135 681 191 1471
Fragments arranged by size:
1597 1471 1135 681 566 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3104 3104
Size: 5860
SfoI GGC'GCC
Cuts at: 2576 2576
Size: 5860
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 3107 1517
Size: 1200 390 4270
Fragments arranged by size:
4270 1200 390
StyI C'CwwG_G
Cuts at: 513 1065 2584 3102 3299 513
Size: 552 1519 518 197 3074
Fragments arranged by size:
3074 1519 552 518 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2215 2293 3155 3887 3904 4057
Size: 1255 498 137 78 862 732 17 153
Cuts at: 4057 4242 4687 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 862 732 498 445 185 153
137 78 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4106 5818 96
Size: 274 80 33 51 1443 2129 1712 138
Fragments arranged by size:
2129 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI
FspAI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SanDI SexAI SrfI SwaI XcmI