Restriction map for
pFN21AE9558
(Circular) (Six-base) MAPSORT of: pFN21AE9558.seq Check: 5088 from: 1 to: 6136
pFN21AE9558 6136 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 2253 3457 2253
Size: 1204 4932
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2779 4199 5583 5851 1632
Size: 1147 1420 1384 268 1917
Fragments arranged by size:
1917 1420 1384 1147 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3362 1184
Size: 2178 3958
AlwNI CAG_nnn'CTG
Cuts at: 2646 3184 3879 5451 2646
Size: 538 695 1572 3331
Fragments arranged by size:
3331 1572 695 538
ApaI G_GGCC'C
Cuts at: 2310 2470 2310
Size: 160 5976
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 3445 3671 3823 5932 1493
Size: 1952 226 152 2109 1697
Fragments arranged by size:
2109 1952 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2334 2780 3461 1870
Size: 73 391 446 681 4545
Fragments arranged by size:
4545 681 446 391 73
BamHI G'GATC_C
Cuts at: 2919 3466 3833 2919
Size: 547 367 5222
Fragments arranged by size:
5222 547 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2253 2265 2822 3128 3249
Size: 325 217 627 466 12 557 306 121
Cuts at: 3249 3258 3457 4912 618
Size: 9 199 1455 1842
Fragments arranged by size:
1842 1455 627 557 466 325 306 217
199 121 12 9
BanII G_rGCy'C
Cuts at: 1391 2310 2470 3431 3455 1391
Size: 919 160 961 24 4072
Fragments arranged by size:
4072 961 919 160 24
BbeI G_GCGC'C
Cuts at: 3262 3262
Size: 6136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2929 928
Size: 971 1030 4135
Fragments arranged by size:
4135 1030 971
BbvCI CC'TCA_GC
Cuts at: 2501 3167 2501
Size: 666 5470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4327 4361 1903
Size: 34 50 34 2306 34 3678
Fragments arranged by size:
3678 2306 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 2952 1548
Size: 1404 4732
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4747 136
Size: 107 122 71 631 605 3075 1525
Fragments arranged by size:
3075 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 3553 3553
Size: 6136
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2470 2827 3010 3131 4197 5353 1790
Size: 520 160 357 183 121 1066 1156 2573
Fragments arranged by size:
2573 1156 1066 520 357 183 160 121
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2356 3867 4825
Size: 416 53 83 399 24 939 1511 958
Cuts at: 4825 442
Size: 1753
Fragments arranged by size:
1753 1511 958 939 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4796 1239
Size: 3557 2579
Bpu10I CC'TnA_GC
Cuts at: 2082 2144 2501 2597 3167 2082
Size: 62 357 96 570 5051
Fragments arranged by size:
5051 570 357 96 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4261 5126 5424 4261
Size: 865 298 4973
Fragments arranged by size:
4973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3383 4799 882
Size: 617 1884 1416 2219
Fragments arranged by size:
2219 1884 1416 617
BsaAI yAC'GTr
Cuts at: 493 1204 3927 493
Size: 711 2723 2702
Fragments arranged by size:
2723 2702 711
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3081 3259 3943 4325
Size: 53 83 186 936 1548 178 684 382
Cuts at: 4325 4960 275
Size: 635 1451
Fragments arranged by size:
1548 1451 936 684 635 382 186 178
83 53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2159 2189 2729 2759 3212
Size: 30 165 30 756 30 540 30 453
Cuts at: 3212 3242 3349 3379 1178
Size: 30 107 30 3935
Fragments arranged by size:
3935 756 540 453 165 107 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 2465 2800 3296 1186
Size: 1210 69 335 496 4026
Fragments arranged by size:
4026 1210 496 335 69
BseYI C'CCAG_C
Cuts at: 2761 2817 3076 3335 5339 5835 2761
Size: 56 259 259 2004 496 3062
Fragments arranged by size:
3062 2004 496 259 259 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2495 2500 2735 3245 1163
Size: 573 759 5 235 510 4054
Fragments arranged by size:
4054 759 573 510 235 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4347 5375 1540
Size: 275 186 1585 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3120 4197 4282 5353 3120
Size: 1077 85 1071 3903
Fragments arranged by size:
3903 1077 1071 85
BsmI GAATG_Cn'
Cuts at: 3647 3740 3647
Size: 93 6043
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2470 2827 3010 3120 3131 3431
Size: 399 520 160 357 183 110 11 300
Cuts at: 3431 3455 4197 4282 5353 1391
Size: 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 357 300 183
160 110 85 24 11
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 2757 2766 3491 844
Size: 1421 492 9 725 3489
Fragments arranged by size:
3489 1421 725 492 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2081 2663 3286 4023 1394
Size: 429 258 582 623 737 3507
Fragments arranged by size:
3507 737 623 582 429 258
BsrDI GCAATG_nn'
Cuts at: 65 1248 2530 2902 4631 4805 65
Size: 1183 1282 372 1729 174 1396
Fragments arranged by size:
1729 1396 1282 1183 372 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4780 4967 1700
Size: 110 2970 187 2869
Fragments arranged by size:
2970 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2415 2436 96
Size: 2319 21 3796
Fragments arranged by size:
3796 2319 21
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 2990 4196 5208 2990
Size: 1206 1012 3918
Fragments arranged by size:
3918 1206 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2758 794
Size: 1964 4172
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 2123 1184
Size: 939 5197
BstXI CCAn_nnnn'nTGG
Cuts at: 2170 2375 2621 2170
Size: 205 246 5685
Fragments arranged by size:
5685 246 205
BstYI r'GATC_y
Cuts at: 1972 2919 3466 3833 4218 4235 5687 5773 5785
Size: 947 547 367 385 17 1452 86 12
Cuts at: 5785 6131 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 947 547 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2163 2229 2685 3325 513
Size: 552 1098 66 456 640 3324
Fragments arranged by size:
3324 1098 640 552 456 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 2512 2859 3652 4446 4466 2512
Size: 347 793 794 20 4182
Fragments arranged by size:
4182 794 793 347 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DraIII CAC_nnn'GTG
Cuts at: 2275 3091 2275
Size: 816 5320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2636 5143 809
Size: 507 1320 2507 1802
Fragments arranged by size:
2507 1802 1320 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2232 2406
Size: 54 1425 50 275 18 377 25 174
Cuts at: 2406 2484 2682 2712 2769 2943 2994 3045 3322
Size: 78 198 30 57 174 51 51 277
Cuts at: 3322 3583 4472 6101 8
Size: 261 889 1629 43
Fragments arranged by size:
1629 1425 889 377 277 275 261 198
174 174 78 57 54 51 51 50
43 30 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3155 3167 4066 1353
Size: 1802 12 899 3423
Fragments arranged by size:
3423 1802 899 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3130 4723 5097 5243 387
Size: 1064 138 1541 1593 374 146 1280
Fragments arranged by size:
1593 1541 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2779 4199 4796 5583 5851 1239
Size: 393 1147 1420 597 787 268 1524
Fragments arranged by size:
1524 1420 1147 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3215 3215
Size: 6136
EcoO109I rG'GnC_Cy
Cuts at: 2071 2466 2467 2497 2601 2740 3181 3306 2071
Size: 395 1 30 104 139 441 125 4901
Fragments arranged by size:
4901 441 395 139 125 104 30 1
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 6136
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 3262 5283 1678
Size: 1584 2021 2531
Fragments arranged by size:
2531 2021 1584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3212 3244 4777 4809 4851
Size: 32 135 32 1640 32 1533 32 42
Cuts at: 4851 4883 1373
Size: 32 2626
Fragments arranged by size:
2626 1640 1533 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6136
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KasI G'GCGC_C
Cuts at: 3258 3258
Size: 6136
KpnI G_GTAC'C
Cuts at: 2257 3461 2257
Size: 1204 4932
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3210 5250 5434 1305
Size: 1905 2040 184 2007
Fragments arranged by size:
2040 2007 1905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 2486 2714 2771 2945
Size: 54 1425 343 576 78 228 57 174
Cuts at: 2945 2996 3047 10
Size: 51 51 3099
Fragments arranged by size:
3099 1425 576 343 228 174 78 57
54 51 51
MslI CAynn'nnrTG
Cuts at: 518 1166 4094 4453 4612 518
Size: 648 2928 359 159 2042
Fragments arranged by size:
2928 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2687 2986 3255 3552 4229 5377 5622 1606
Size: 1081 299 269 297 677 1148 245 2120
Fragments arranged by size:
2120 1148 1081 677 299 297 269 245
NarI GG'CG_CC
Cuts at: 3259 3259
Size: 6136
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5584
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 3494 5039 1132
Size: 97 2265 1545 2229
Fragments arranged by size:
2265 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2461 2847 3124 1348
Size: 1113 386 277 4360
Fragments arranged by size:
4360 1113 386 277
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3091 1108
Size: 1983 4153
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2601 2071
Size: 530 5606
PshAI GACnn'nnGTC
Cuts at: 1613 2130 1613
Size: 517 5619
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PspOMI G'GGCC_C
Cuts at: 2306 2466 2306
Size: 160 5976
PstI C_TGCA'G
Cuts at: 830 3351 3488 830
Size: 2521 137 3478
Fragments arranged by size:
3478 2521 137
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2986 2986
Size: 6136
SacII CC_GC'GG
Cuts at: 2688 2688
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SapI GCTCTTCn'nnn_
Cuts at: 3155 3155
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SfcI C'TryA_G
Cuts at: 826 1045 2449 2608 3347 3484 4619 5300 5491
Size: 219 1404 159 739 137 1135 681 191
Cuts at: 5491 826
Size: 1471
Fragments arranged by size:
1471 1404 1135 739 681 219 191 159
137
SfoI GGC'GCC
Cuts at: 3260 3260
Size: 6136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 2336 3463 1872
Size: 464 1127 4545
Fragments arranged by size:
4545 1127 464
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4240 5141 5403 820
Size: 197 926 2297 901 262 1553
Fragments arranged by size:
2297 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6136
StyI C'CwwG_G
Cuts at: 513 1065 2261 2345 2481 3396 3575 513
Size: 552 1196 84 136 915 179 3074
Fragments arranged by size:
3074 1196 915 552 179 136 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2311 4163 4180 4333 4518 4963 325
Size: 1255 731 1852 17 153 185 445 1498
Fragments arranged by size:
1852 1498 1255 731 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2415 2436 4382
Size: 274 80 33 51 1443 438 21 1946
Cuts at: 4382 6094 96
Size: 1712 138
Fragments arranged by size:
1946 1712 1443 438 274 138 80 51
33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 3349 3381 6096 6128 2159
Size: 32 1158 32 2715 32 2167
Fragments arranged by size:
2715 2167 1158 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 2575
Size: 6136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 2334 3461 1870
Size: 464 1127 4545
Fragments arranged by size:
4545 1127 464
XmnI GAAnn'nnTTC
Cuts at: 4265 4265
Size: 6136
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI