Restriction map for
pFN21AE9555
(Circular) (Six-base) MAPSORT of: pFN21AE9555.seq Check: 4516 from: 1 to: 8245
pFN21AE9555 8245 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6055 278
Size: 53 83 186 936 4519 2468
Fragments arranged by size:
4519 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4191 5588 1937
Size: 2254 1397 4594
Fragments arranged by size:
4594 2254 1397
Acc65I G'GTAC_C
Cuts at: 5566 5566
Size: 8245
AclI AA'CG_TT
Cuts at: 1563 3453 6372 6745 1563
Size: 1890 2919 373 3063
Fragments arranged by size:
3063 2919 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 2568 2807 3050 3926 5398 6308 7692
Size: 714 222 239 243 876 1472 910 1384
Cuts at: 7692 7960 1632
Size: 268 1917
Fragments arranged by size:
1917 1472 1384 910 876 714 268 243
239 222
AfeI AGC'GCT
Cuts at: 4819 4819
Size: 8245
AflII C'TTAA_G
Cuts at: 820 1017 4630 820
Size: 197 3613 4435
Fragments arranged by size:
4435 3613 197
AflIII A'CryG_T
Cuts at: 1203 7144 8121 1203
Size: 5941 977 1327
Fragments arranged by size:
5941 1327 977
AgeI A'CCGG_T
Cuts at: 7076 7076
Size: 8245
AhdI GACnn_n'nnGTC
Cuts at: 6974 6974
Size: 8245
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4475 1184
Size: 3291 4954
AlwNI CAG_nnn'CTG
Cuts at: 4693 4705 5260 5988 7560 4693
Size: 12 555 728 1572 5378
Fragments arranged by size:
5378 1572 728 555 12
ApaI G_GGCC'C
Cuts at: 4663 4878 4663
Size: 215 8030
ApaLI G'TGCA_C
Cuts at: 2061 2654 6302 7458 2061
Size: 593 3648 1156 2848
Fragments arranged by size:
3648 2848 1156 593
ApoI r'AATT_y
Cuts at: 1493 2482 2897 3074 3412 5554 5780 5932 8041
Size: 989 415 177 338 2142 226 152 2109
Cuts at: 8041 1493
Size: 1697
Fragments arranged by size:
2142 2109 1697 989 415 338 226 177
152
AseI AT'TA_AT
Cuts at: 160 1032 6799 160
Size: 872 5767 1606
Fragments arranged by size:
5767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8245
AvaI C'yCGr_G
Cuts at: 1870 1943 2817 4877 5328 5351 5431 5570 1870
Size: 73 874 2060 451 23 80 139 4545
Fragments arranged by size:
4545 2060 874 451 139 80 73 23
AvrII C'CTAG_G
Cuts at: 4216 4444 4216
Size: 228 8017
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2628 2661 3813 3846 3880 3913 2628
Size: 33 1152 33 34 33 6960
Fragments arranged by size:
6960 1152 34 33 33 33
BamHI G'GATC_C
Cuts at: 4017 5575 5942 4017
Size: 1558 367 6320
Fragments arranged by size:
6320 1558 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2877 4859 4942 5177 5566
Size: 325 217 627 1090 1982 83 235 389
Cuts at: 5566 7021 618
Size: 1455 1842
Fragments arranged by size:
1982 1842 1455 1090 627 389 325 235
217 83
BanII G_rGCy'C
Cuts at: 1391 2122 3237 4616 4663 4878 5000 5075 5133
Size: 731 1115 1379 47 215 122 75 58
Cuts at: 5133 5339 5534 5564 1391
Size: 206 195 30 4072
Fragments arranged by size:
4072 1379 1115 731 215 206 195 122
75 58 47 30
BbeI G_GCGC'C
Cuts at: 2881 4863 4946 5181 2881
Size: 1982 83 235 5945
Fragments arranged by size:
5945 1982 235 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3796 928
Size: 971 1897 5377
Fragments arranged by size:
5377 1897 971
BbvCI CC'TCA_GC
Cuts at: 4926 4926
Size: 8245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6436 6470 1903
Size: 34 4499 34 3678
Fragments arranged by size:
4499 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5489 6137 7347 4232
Size: 1257 648 1210 5130
Fragments arranged by size:
5130 1257 1210 648
BclI T'GATC_A
Cuts at: 1548 3204 1548
Size: 1656 6589
BfrBI ATG'CAT
Cuts at: 4110 4165 4540 4110
Size: 55 375 7815
Fragments arranged by size:
7815 375 55
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4439 4951 6856
Size: 107 122 71 631 605 2767 512 1905
Cuts at: 6856 136
Size: 1525
Fragments arranged by size:
2767 1905 1525 631 605 512 122 107
71
BglII A'GATC_T
Cuts at: 3527 4041 8240 3527
Size: 514 4199 3532
Fragments arranged by size:
4199 3532 514
BlpI GC'TnA_GC
Cuts at: 2592 4982 5662 2592
Size: 2390 680 5175
Fragments arranged by size:
5175 2390 680
Bme1580I G_kGCm'C
Cuts at: 1790 2065 2658 4081 4663 4878 6306 7462 1790
Size: 275 593 1423 582 215 1428 1156 2573
Fragments arranged by size:
2573 1428 1423 1156 593 582 275 215
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2285 3354 3674
Size: 416 53 83 399 24 868 1069 320
Cuts at: 3674 3704 5976 6934 442
Size: 30 2272 958 1753
Fragments arranged by size:
2272 1753 1069 958 868 416 399 320
83 53 30 24
BmtI G_CTAG'C
Cuts at: 1056 4364 1056
Size: 3308 4937
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2369 5152 2369
Size: 2783 5462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2440 2474 3375 5399 6905 1239
Size: 1201 34 901 2024 1506 2579
Fragments arranged by size:
2579 2024 1506 1201 901 34
Bpu10I CC'TnA_GC
Cuts at: 4926 4926
Size: 8245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 6370 7235 7533 3826
Size: 2544 865 298 4538
Fragments arranged by size:
4538 2544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 6908 882
Size: 617 2466 2943 2219
Fragments arranged by size:
2943 2466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4656 6036 493
Size: 711 3452 1380 2702
Fragments arranged by size:
3452 2702 1380 711
BsaBI GATnn'nnATC
Cuts at: 2726 2952 3024 8239 2726
Size: 226 72 5215 2732
Fragments arranged by size:
5215 2732 226 72
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2878 4860 4890 4943
Size: 53 83 186 936 1345 1982 30 53
Cuts at: 4943 5172 5178 6052 6434 7069 275
Size: 229 6 874 382 635 1451
Fragments arranged by size:
1982 1451 1345 936 874 635 382 229
186 83 53 53 30 6
BsaWI w'CCGG_w
Cuts at: 6678 7076 7350 7497 7908 6678
Size: 398 274 147 411 7015
Fragments arranged by size:
7015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3611 3641 1178
Size: 30 165 30 2208 30 5782
Fragments arranged by size:
5782 2208 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 2392 2395 4606 4943 5053 5396 5524
Size: 906 300 3 2211 337 110 343 128
Cuts at: 5524 1186
Size: 3907
Fragments arranged by size:
3907 2211 906 343 337 300 128 110
3
BseYI C'CCAG_C
Cuts at: 2922 3967 4080 4820 5162 5315 7448 7944 2922
Size: 1045 113 740 342 153 2133 496 3223
Fragments arranged by size:
3223 2133 1045 740 496 342 153 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3556 4249 5018 1163
Size: 573 1820 693 769 4390
Fragments arranged by size:
4390 1820 769 693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4749 4955 5346 5364 5695 6456
Size: 275 186 2748 206 391 18 331 761
Cuts at: 6456 7484 1540
Size: 1028 2301
Fragments arranged by size:
2748 2301 1028 761 391 331 275 206
186 18
BsiHKAI G_wGCw'C
Cuts at: 2065 2122 2658 4775 4935 5133 5441 5534 6306
Size: 57 536 2117 160 198 308 93 772
Cuts at: 6306 6391 7462 2065
Size: 85 1071 2848
Fragments arranged by size:
2848 2117 1071 772 536 308 198 160
93 85 57
BsmI GAATG_Cn'
Cuts at: 2199 2305 4179 5756 5849 2199
Size: 106 1874 1577 93 4595
Fragments arranged by size:
4595 1874 1577 106 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5493 5493
Size: 8245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 2122 2658 3237 4081 4616 4663
Size: 399 275 57 536 579 844 535 47
Cuts at: 4663 4775 4878 4935 5000 5075 5133 5339 5441
Size: 112 103 57 65 75 58 206 102
Cuts at: 5441 5534 5564 6306 6391 7462 1391
Size: 93 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 844 742 579 536 535 399
275 206 112 103 102 93 85 75
65 58 57 57 47 30
BspEI T'CCGG_A
Cuts at: 7908 7908
Size: 8245
BspHI T'CATG_A
Cuts at: 2161 2568 4065 6134 7864 2161
Size: 407 1497 2069 1730 2542
Fragments arranged by size:
2542 2069 1730 1497 407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4355 5600 844
Size: 3511 1245 3489
Fragments arranged by size:
3511 3489 1245
BsrBI CCG'CTC
Cuts at: 1394 1823 2418 5185 6132 1394
Size: 429 595 2767 947 3507
Fragments arranged by size:
3507 2767 947 595 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3744 3909 6740 6914 65
Size: 1183 2496 165 2831 174 1396
Fragments arranged by size:
2831 2496 1396 1183 174 165
BsrFI r'CCGG_y
Cuts at: 1700 1810 4862 4892 5263 5345 6889 7076 1700
Size: 110 3052 30 371 82 1544 187 2869
Fragments arranged by size:
3052 2869 1544 371 187 110 82 30
BsrGI T'GTAC_A
Cuts at: 96 3605 4338 4470 96
Size: 3509 733 132 3871
Fragments arranged by size:
3871 3509 733 132
BssHII G'CGCG_C
Cuts at: 1925 5051 5286 1925
Size: 3126 235 4884
Fragments arranged by size:
4884 3126 235
BssSI C'ACGA_G
Cuts at: 6305 7317 6305
Size: 1012 7233
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3657 794
Size: 2863 5382
BstBI TT'CG_AA
Cuts at: 7049 7049
Size: 8245
BstEII G'GTnAC_C
Cuts at: 1184 3731 5101 1184
Size: 2547 1370 4328
Fragments arranged by size:
4328 2547 1370
BstYI r'GATC_y
Cuts at: 1972 2536 3527 4017 4041 4577 5214 5575 5942
Size: 564 991 490 24 536 637 361 367
Cuts at: 5942 6327 6344 7796 7882 7894 8240 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 991 637 564 536 490 385
367 361 346 86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 2607 4527 4783 4836 5154
Size: 552 939 49 554 1920 256 53 318
Cuts at: 5154 5295 5340 513
Size: 141 45 3418
Fragments arranged by size:
3418 1920 939 554 552 318 256 141
53 49 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2713 487
Size: 683 56 1487 6019
Fragments arranged by size:
6019 1487 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 3544 5101 5761 6555 6575 2920
Size: 624 1557 660 794 20 4590
Fragments arranged by size:
4590 1557 794 660 624 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3772 3807 529
Size: 35 3208 35 4967
Fragments arranged by size:
4967 3208 35 35
DraI TTT'AAA
Cuts at: 5549 5549
Size: 8245
DraIII CAC_nnn'GTG
Cuts at: 4410 4410
Size: 8245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7252 809
Size: 507 5936 1802
Fragments arranged by size:
5936 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2868 3038 3780
Size: 54 1425 50 275 18 1038 170 742
Cuts at: 3780 4482 4746 4917 4952 5001 5115 5265 5343
Size: 702 264 171 35 49 114 150 78
Cuts at: 5343 5361 5692 6581 8210 8
Size: 18 331 889 1629 43
Fragments arranged by size:
1629 1425 1038 889 742 702 331 275
264 171 170 150 114 78 54 50
49 43 35 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4746 4952 5343 5361 5692 1537
Size: 275 2934 206 391 18 331 4090
Fragments arranged by size:
4090 2934 391 331 275 206 18
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2413 2618 4598 4667 6175 1353
Size: 1008 52 205 1980 69 1508 3423
Fragments arranged by size:
3423 1980 1508 1008 205 69 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6832 7206 7352 387
Size: 1064 138 5243 374 146 1280
Fragments arranged by size:
5243 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2120 5131 5532 2120
Size: 3011 401 4833
Fragments arranged by size:
4833 3011 401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 2440 2474 2568 2807 3050 3375
Size: 393 714 94 34 94 239 243 325
Cuts at: 3375 3926 5398 5399 6308 6905 7692 7960 1239
Size: 551 1472 1 909 597 787 268 1524
Fragments arranged by size:
1524 1472 909 787 714 597 551 393
325 268 243 239 94 94 34 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3844 3844
Size: 8245
EcoRI G'AATT_C
Cuts at: 5554 5932 5554
Size: 378 7867
EcoRV GAT'ATC
Cuts at: 2954 2954
Size: 8245
FspI TGC'GCA
Cuts at: 4505 4535 4855 6751 4505
Size: 30 320 1896 5999
Fragments arranged by size:
5999 1896 320 30
FspAI rTGC'GCAy
Cuts at: 4535 4535
Size: 8245
HaeII r_GCGC'y
Cuts at: 1678 2881 4512 4705 4797 4803 4821 4863 4907
Size: 1203 1631 193 92 6 18 42 44
Cuts at: 4907 4946 5181 7392 1678
Size: 39 235 2211 2531
Fragments arranged by size:
2531 2211 1631 1203 235 193 92 44
42 39 18 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2387 2419 3189 3221 3348
Size: 32 135 32 815 32 770 32 127
Cuts at: 3348 3380 4541 4573 5012 5044 6886 6918 6960
Size: 32 1161 32 439 32 1842 32 42
Cuts at: 6960 6992 1373
Size: 32 2626
Fragments arranged by size:
2626 1842 1161 815 770 439 135 127
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4594 5589 5835 669
Size: 988 281 2656 995 246 3079
Fragments arranged by size:
3079 2656 995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8245
HpaI GTT'AAC
Cuts at: 5835 5835
Size: 8245
KasI G'GCGC_C
Cuts at: 2877 4859 4942 5177 2877
Size: 1982 83 235 5945
Fragments arranged by size:
5945 1982 235 83
KpnI G_GTAC'C
Cuts at: 5570 5570
Size: 8245
MfeI C'AATT_G
Cuts at: 3297 5844 3297
Size: 2547 5698
MluI A'CGCG_T
Cuts at: 8121 8121
Size: 8245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3436 3578 7359 7543 1305
Size: 2131 142 3781 184 2007
Fragments arranged by size:
3781 2131 2007 184 142
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2870 3782 4484 10
Size: 54 1425 343 1038 912 702 3771
Fragments arranged by size:
3771 1425 1038 912 702 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2849 3248 4406 4457 5231 6203 6562
Size: 648 1683 399 1158 51 774 972 359
Cuts at: 6562 6721 518
Size: 159 2042
Fragments arranged by size:
2042 1683 1158 972 774 648 399 359
159 51
MspA1I CmG'CkG
Cuts at: 1606 3591 3752 5156 5297 5342 5661 6338 7486
Size: 1985 161 1404 141 45 319 677 1148
Cuts at: 7486 7731 1606
Size: 245 2120
Fragments arranged by size:
2120 1985 1404 1148 677 319 245 161
141 45
NaeI GCC'GGC
Cuts at: 4864 4894 5265 4864
Size: 30 371 7844
Fragments arranged by size:
7844 371 30
NarI GG'CG_CC
Cuts at: 2878 4860 4943 5178 2878
Size: 1982 83 235 5945
Fragments arranged by size:
5945 1982 235 83
NcoI C'CATG_G
Cuts at: 513 1065 2004 2607 513
Size: 552 939 603 6151
Fragments arranged by size:
6151 939 603 552
NdeI CA'TA_TG
Cuts at: 387 4106 387
Size: 3719 4526
NgoMIV G'CCGG_C
Cuts at: 4862 4892 5263 4862
Size: 30 371 7844
Fragments arranged by size:
7844 371 30
NheI G'CTAG_C
Cuts at: 1052 4360 1052
Size: 3308 4937
NotI GC'GGCC_GC
Cuts at: 4952 5361 5692 4952
Size: 409 331 7505
Fragments arranged by size:
7505 409 331
NsiI A_TGCA'T
Cuts at: 4112 4167 4542 4112
Size: 55 375 7815
Fragments arranged by size:
7815 375 55
NspI r_CATG'y
Cuts at: 1132 1229 2098 3247 3651 3660 4456 4540 4544
Size: 97 869 1149 404 9 796 84 4
Cuts at: 4544 5603 7148 1132
Size: 1059 1545 2229
Fragments arranged by size:
2229 1545 1149 1059 869 796 404 97
84 9 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8245
PciI A'CATG_T
Cuts at: 7144 7144
Size: 8245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3302 1108
Size: 2194 6051
PmeI GTTT'AAAC
Cuts at: 5549 5549
Size: 8245
PmlI CAC'GTG
Cuts at: 4656 4656
Size: 8245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6314 6346 7827 7859 6314
Size: 32 1481 32 6700
Fragments arranged by size:
6700 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8245
PsiI TTA'TAA
Cuts at: 4142 5815 4142
Size: 1673 6572
PspOMI G'GGCC_C
Cuts at: 4659 4874 4659
Size: 215 8030
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 8213
PstI C_TGCA'G
Cuts at: 830 5597 830
Size: 4767 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8245
PvuII CAG'CTG
Cuts at: 3591 3752 3591
Size: 161 8084
RsrII CG'GwC_CG
Cuts at: 2182 2182
Size: 8245
SacI G_AGCT'C
Cuts at: 2122 5133 5534 2122
Size: 3011 401 4833
Fragments arranged by size:
4833 3011 401
SacII CC_GC'GG
Cuts at: 5157 5298 5343 5157
Size: 141 45 8059
Fragments arranged by size:
8059 141 45
SalI G'TCGA_C
Cuts at: 1936 5587 1936
Size: 3651 4594
SapI GCTCTTCn'nnn_
Cuts at: 4667 4667
Size: 8245
SbfI CC_TGCA'GG
Cuts at: 5597 5597
Size: 8245
ScaI AGT'ACT
Cuts at: 6493 8205 6493
Size: 1712 6533
SfcI C'TryA_G
Cuts at: 826 1045 2463 2749 3798 5593 6728 7409 7600
Size: 219 1418 286 1049 1795 1135 681 191
Cuts at: 7600 826
Size: 1471
Fragments arranged by size:
1795 1471 1418 1135 1049 681 286 219
191
SfoI GGC'GCC
Cuts at: 2879 4861 4944 5179 2879
Size: 1982 83 235 5945
Fragments arranged by size:
5945 1982 235 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8245
SmaI CCC'GGG
Cuts at: 1872 2819 5330 5572 1872
Size: 947 2511 242 4545
Fragments arranged by size:
4545 2511 947 242
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3841 4630 6349 7250 7512 820
Size: 197 926 1898 789 1719 901 262 1553
Fragments arranged by size:
1898 1719 1553 926 901 789 262 197
SnaBI TAC'GTA
Cuts at: 493 6036 493
Size: 5543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8245
SphI G_CATG'C
Cuts at: 1132 3660 4540 5603 1132
Size: 2528 880 1063 3774
Fragments arranged by size:
3774 2528 1063 880
SspI AAT'ATT
Cuts at: 5 52 2564 6169 5
Size: 47 2512 3605 2081
Fragments arranged by size:
3605 2512 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8245
StyI C'CwwG_G
Cuts at: 513 1065 2004 2607 2811 3062 3769 4216 4444
Size: 552 939 603 204 251 707 447 228
Cuts at: 4444 4899 5684 513
Size: 455 785 3074
Fragments arranged by size:
3074 939 785 707 603 552 455 447
251 228 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4408 6272 6289 6442 6627 7072 325
Size: 1255 2828 1864 17 153 185 445 1498
Fragments arranged by size:
2828 1864 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3605 3642 3712
Size: 274 80 33 51 1443 1628 37 70
Cuts at: 3712 4338 4470 6491 8203 96
Size: 626 132 2021 1712 138
Fragments arranged by size:
2021 1712 1628 1443 626 274 138 132
80 70 51 37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8205 8237 8205
Size: 32 8213
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8245
XbaI T'CTAG_A
Cuts at: 5581 5581
Size: 8245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2818 2878 3220 3751 4525 5374 2272
Size: 546 60 342 531 774 849 5143
Fragments arranged by size:
5143 849 774 546 531 342 60
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8245
XmaI C'CCGG_G
Cuts at: 1870 2817 5328 5570 1870
Size: 947 2511 242 4545
Fragments arranged by size:
4545 2511 947 242
XmnI GAAnn'nnTTC
Cuts at: 3111 6374 3111
Size: 3263 4982
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6053 276
Size: 53 83 186 936 4519 2468
Fragments arranged by size:
4519 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BsiWI BstXI BstZ17I Bsu36I EcoO109I
FalI FseI NruI PacI PfoI PpuMI PspXI SanDI
SexAI SfiI SrfI SwaI