Restriction map for
pFN21AE9552
(Circular) (Six-base) MAPSORT of: pFN21AE9552.seq Check: 5398 from: 1 to: 5869
pFN21AE9552 5869 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2597 2869 2597
Size: 272 5597
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2307 3679 278
Size: 53 83 186 936 771 1372 2468
Fragments arranged by size:
2468 1372 936 771 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3212 1937
Size: 1275 4594
Acc65I G'GTAC_C
Cuts at: 3190 3190
Size: 5869
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3932 5316 5584 1632
Size: 2300 1384 268 1917
Fragments arranged by size:
2300 1917 1384 268
AfeI AGC'GCT
Cuts at: 3002 3002
Size: 5869
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 3070 4768 5745 1203
Size: 1867 1698 977 1327
Fragments arranged by size:
1867 1698 1327 977
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5869
AlwNI CAG_nnn'CTG
Cuts at: 3612 5184 3612
Size: 1572 4297
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 3178 3404 3556 5665 1493
Size: 1685 226 152 2109 1697
Fragments arranged by size:
2109 1697 1685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2423 3057 4423 160
Size: 872 1391 634 1366 1606
Fragments arranged by size:
1606 1391 1366 872 634
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 3194 1870
Size: 73 1251 4545
Fragments arranged by size:
4545 1251 73
BamHI G'GATC_C
Cuts at: 2848 3062 3199 3566 2848
Size: 214 137 367 5151
Fragments arranged by size:
5151 367 214 137
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2821 3190 4645 618
Size: 325 217 627 447 587 369 1455 1842
Fragments arranged by size:
1842 1455 627 587 447 369 325 217
BanII G_rGCy'C
Cuts at: 1391 3188 1391
Size: 1797 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2551 3016 928
Size: 971 652 465 3781
Fragments arranged by size:
3781 971 652 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4060 4094 1903
Size: 34 2123 34 3678
Fragments arranged by size:
3678 2123 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 4971 3761
Size: 1210 4659
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4480 136
Size: 107 122 71 631 605 2808 1525
Fragments arranged by size:
2808 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5864 5864
Size: 5869
BlpI GC'TnA_GC
Cuts at: 3153 3286 3153
Size: 133 5736
Bme1580I G_kGCm'C
Cuts at: 1790 2824 3930 5086 1790
Size: 1034 1106 1156 2573
Fragments arranged by size:
2573 1156 1106 1034
BmgBI CAC'GTC
Cuts at: 1318 2966 1318
Size: 1648 4221
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3153 3600 4558
Size: 416 53 83 399 24 1736 447 958
Cuts at: 4558 442
Size: 1753
Fragments arranged by size:
1753 1736 958 447 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2739 2739
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4529 1239
Size: 3290 2579
Bpu10I CC'TnA_GC
Cuts at: 2153 2661 2153
Size: 508 5361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3994 4859 5157 3994
Size: 865 298 4706
Fragments arranged by size:
4706 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2759 4532 882
Size: 617 1260 1773 2219
Fragments arranged by size:
2219 1773 1260 617
BsaAI yAC'GTr
Cuts at: 493 1204 2550 3660 493
Size: 711 1346 1110 2702
Fragments arranged by size:
2702 1346 1110 711
BsaBI GATnn'nnATC
Cuts at: 2102 5863 2102
Size: 3761 2108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 3676 4058 4693
Size: 53 83 186 936 771 1372 382 635
Cuts at: 4693 275
Size: 1451
Fragments arranged by size:
1451 1372 936 771 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4302 4700 4974 5121 5532 4302
Size: 398 274 147 411 4639
Fragments arranged by size:
4639 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3101 3131 1178
Size: 30 165 30 1698 30 3916
Fragments arranged by size:
3916 1698 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2612 2698 1186
Size: 865 561 86 4357
Fragments arranged by size:
4357 865 561 86
BseYI C'CCAG_C
Cuts at: 2139 2428 2439 2817 5072 5568 2139
Size: 289 11 378 2255 496 2440
Fragments arranged by size:
2440 2255 496 378 289 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5296
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3319 4080 5108 1540
Size: 275 186 1318 761 1028 2301
Fragments arranged by size:
2301 1318 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3930 4015 5086 3930
Size: 85 1071 4713
Fragments arranged by size:
4713 1071 85
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2824 3188 3930 4015 5086 1391
Size: 399 1034 364 742 85 1071 2174
Fragments arranged by size:
2174 1071 1034 742 399 364 85
BspEI T'CCGG_A
Cuts at: 5532 5532
Size: 5869
BspHI T'CATG_A
Cuts at: 3758 5488 3758
Size: 1730 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2597 2869 3224 844
Size: 1280 473 272 355 3489
Fragments arranged by size:
3489 1280 473 355 272
BsrBI CCG'CTC
Cuts at: 1394 1823 3756 1394
Size: 429 1933 3507
Fragments arranged by size:
3507 1933 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4364 4538 65
Size: 1183 3116 174 1396
Fragments arranged by size:
3116 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4513 4700 1700
Size: 110 2703 187 2869
Fragments arranged by size:
2869 2703 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5869
BssSI C'ACGA_G
Cuts at: 3929 4941 3929
Size: 1012 4857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5869
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2845 2245
Size: 600 5269
BstYI r'GATC_y
Cuts at: 1972 2200 2679 2848 3062 3199 3566 3951 3968
Size: 228 479 169 214 137 367 385 17
Cuts at: 3968 5420 5506 5518 5864 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 479 385 367 346 228 214
169 137 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4378
Fragments arranged by size:
4378 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2401 2735 487
Size: 683 56 1175 334 3621
Fragments arranged by size:
3621 1175 683 334 56
BtsI GCAGTG_nn'
Cuts at: 3093 3385 4179 4199 3093
Size: 292 794 20 4763
Fragments arranged by size:
4763 794 292 20
ClaI AT'CG_AT
Cuts at: 1554 2324 1554
Size: 770 5099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2867 4876 809
Size: 507 1551 2009 1802
Fragments arranged by size:
2009 1802 1551 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 3100 3316
Size: 54 1425 50 275 18 270 1000 216
Cuts at: 3316 4205 5834 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1000 889 275 270 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3316 1537
Size: 275 1504 4090
Fragments arranged by size:
4090 1504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3799 1353
Size: 2446 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4456 4830 4976 387
Size: 1064 138 2867 374 146 1280
Fragments arranged by size:
2867 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3932 4529 5316 5584 1239
Size: 393 2300 597 787 268 1524
Fragments arranged by size:
2300 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3178 3556 3178
Size: 378 5491
FspI TGC'GCA
Cuts at: 2414 4375 2414
Size: 1961 3908
HaeII r_GCGC'y
Cuts at: 1678 3004 5016 1678
Size: 1326 2012 2531
Fragments arranged by size:
2531 2012 1326
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2392 2424 3103 3135 4510
Size: 32 135 32 820 32 679 32 1375
Cuts at: 4510 4542 4584 4616 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1375 820 679 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2569 3213 3459 669
Size: 988 281 631 644 246 3079
Fragments arranged by size:
3079 988 644 631 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 3194 3194
Size: 5869
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4983 5167 1305
Size: 3678 184 2007
Fragments arranged by size:
3678 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2102 3102 10
Size: 54 1425 343 270 1000 2777
Fragments arranged by size:
2777 1425 1000 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2795 3827 4186 4345 518
Size: 648 1629 1032 359 159 2042
Fragments arranged by size:
2042 1629 1032 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3285 3962 5110 5355 1606
Size: 1679 677 1148 245 2120
Fragments arranged by size:
2120 1679 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4378
Fragments arranged by size:
4378 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 2672 3074 3227 4772 1132
Size: 97 1443 402 153 1545 2229
Fragments arranged by size:
2229 1545 1443 402 153 97
PasI CC'CwG_GG
Cuts at: 1348 2873 1348
Size: 1525 4344
PciI A'CATG_T
Cuts at: 3070 4768 3070
Size: 1698 4171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2383 1108
Size: 1275 4594
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 3938 3970 5451 5483 2528
Size: 32 1378 32 1481 32 2914
Fragments arranged by size:
2914 1481 1378 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PstI C_TGCA'G
Cuts at: 830 3221 830
Size: 2391 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SbfI CC_TGCA'GG
Cuts at: 3221 3221
Size: 5869
ScaI AGT'ACT
Cuts at: 4117 5829 4117
Size: 1712 4157
SfcI C'TryA_G
Cuts at: 826 1045 2389 3217 4352 5033 5224 826
Size: 219 1344 828 1135 681 191 1471
Fragments arranged by size:
1471 1344 1135 828 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 3196 1872
Size: 1324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3973 4874 5136 820
Size: 197 926 2030 901 262 1553
Fragments arranged by size:
2030 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2550 3660 493
Size: 2057 1110 2702
Fragments arranged by size:
2702 2057 1110
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 2672 3227 1132
Size: 1540 555 3774
Fragments arranged by size:
3774 1540 555
SspI AAT'ATT
Cuts at: 5 52 2842 3793 5
Size: 47 2790 951 2081
Fragments arranged by size:
2790 2081 951 47
StuI AGG'CCT
Cuts at: 1517 2722 1517
Size: 1205 4664
StyI C'CwwG_G
Cuts at: 513 1065 2004 3308 513
Size: 552 939 1304 3074
Fragments arranged by size:
3074 1304 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3896 3913 4066 4251 4696 325
Size: 1255 2316 17 153 185 445 1498
Fragments arranged by size:
2316 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3073 4115 5827
Size: 274 80 33 51 1443 1096 1042 1712
Cuts at: 5827 96
Size: 138
Fragments arranged by size:
1712 1443 1096 1042 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3101 3133 5829 5861 3101
Size: 32 2696 32 3109
Fragments arranged by size:
3109 2696 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5869
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 3194 1870
Size: 1324 4545
XmnI GAAnn'nnTTC
Cuts at: 3013 3998 3013
Size: 985 4884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2305 3677 276
Size: 53 83 186 936 771 1372 2468
Fragments arranged by size:
2468 1372 936 771 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI