Restriction map for
pFN21AE9550
(Circular) (Six-base) MAPSORT of: pFN21AE9550.seq Check: 9498 from: 1 to: 5899
pFN21AE9550 5899 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2672 2737 2672
Size: 65 5834
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3962 5346 5614 1632
Size: 2330 1384 268 1917
Fragments arranged by size:
2330 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2915 820
Size: 197 1898 3804
Fragments arranged by size:
3804 1898 197
AflIII A'CryG_T
Cuts at: 1203 2034 2390 4798 5775 1203
Size: 831 356 2408 977 1327
Fragments arranged by size:
2408 1327 977 831 356
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 2267 1166
Size: 1101 4798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 2502 2752 2866 3642 5214 2502
Size: 250 114 776 1572 3187
Fragments arranged by size:
3187 1572 776 250 114
ApaI G_GGCC'C
Cuts at: 2837 2873 2837
Size: 36 5863
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2558 3224 1870
Size: 73 128 487 666 4545
Fragments arranged by size:
4545 666 487 128 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2081 2768 2886 3220
Size: 325 217 627 260 34 687 118 334
Cuts at: 3220 4675 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 687 627 334 325 260 217
118 34
BanII G_rGCy'C
Cuts at: 1391 2837 2873 2882 2929 2987 3218 1391
Size: 1446 36 9 47 58 231 4072
Fragments arranged by size:
4072 1446 231 58 47 36 9
BbeI G_GCGC'C
Cuts at: 2085 2772 2085
Size: 687 5212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4928
BbvCI CC'TCA_GC
Cuts at: 2495 2495
Size: 5899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4090 4124 1903
Size: 34 50 34 2069 34 3678
Fragments arranged by size:
3678 2069 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 2697 2777
Size: 107 122 71 631 605 583 442 80
Cuts at: 2777 2877 4510 136
Size: 100 1633 1525
Fragments arranged by size:
1633 1525 631 605 583 442 122 107
100 80 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2837 2873 3960 5116 1790
Size: 260 787 36 1087 1156 2573
Fragments arranged by size:
2573 1156 1087 787 260 36
BmgBI CAC'GTC
Cuts at: 1318 2269 1318
Size: 951 4948
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2096 3630 4588
Size: 416 53 83 399 24 679 1534 958
Cuts at: 4588 442
Size: 1753
Fragments arranged by size:
1753 1534 958 679 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 4559 1239
Size: 1385 1935 2579
Fragments arranged by size:
2579 1935 1385
Bpu10I CC'TnA_GC
Cuts at: 2495 2495
Size: 5899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4024 4889 5187 4024
Size: 865 298 4736
Fragments arranged by size:
4736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2380 2791 4562 882
Size: 617 881 411 1771 2219
Fragments arranged by size:
2219 1771 881 617 411
BsaAI yAC'GTr
Cuts at: 493 1204 2391 2431 3690 493
Size: 711 1187 40 1259 2702
Fragments arranged by size:
2702 1259 1187 711 40
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2127 2769 3706
Size: 53 83 186 936 549 45 642 937
Cuts at: 3706 4088 4723 275
Size: 382 635 1451
Fragments arranged by size:
1451 937 936 642 635 549 382 186
83 53 45
BsaWI w'CCGG_w
Cuts at: 2024 4332 4730 5004 5151 5562 2024
Size: 2308 398 274 147 411 2361
Fragments arranged by size:
2361 2308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2933 2963 1178
Size: 30 165 30 1530 30 4114
Fragments arranged by size:
4114 1530 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5899
BseYI C'CCAG_C
Cuts at: 2167 2325 2332 2591 2829 2866 5102 5598 2167
Size: 158 7 259 238 37 2236 496 2468
Fragments arranged by size:
2468 2236 496 259 238 158 37 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2441 2528 3077 1163
Size: 573 666 39 87 549 3985
Fragments arranged by size:
3985 666 573 549 87 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2805 3349 4110 5138 1540
Size: 275 186 804 544 761 1028 2301
Fragments arranged by size:
2301 1028 804 761 544 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 3960 4045 5116 2538
Size: 1422 85 1071 3321
Fragments arranged by size:
3321 1422 1071 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2538 2837 2873 2882 2929 2987
Size: 399 260 488 299 36 9 47 58
Cuts at: 2987 3218 3960 4045 5116 1391
Size: 231 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 488 399 299 260 231
85 58 47 36 9
BspEI T'CCGG_A
Cuts at: 2024 5562 2024
Size: 3538 2361
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2362 2637 2652 2672 2737 3254 844
Size: 1518 275 15 20 65 517 3489
Fragments arranged by size:
3489 1518 517 275 65 20 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2143 3786 1394
Size: 429 320 1643 3507
Fragments arranged by size:
3507 1643 429 320
BsrDI GCAATG_nn'
Cuts at: 65 1248 4394 4568 65
Size: 1183 3146 174 1396
Fragments arranged by size:
3146 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2050 2530 3051 4543 4730 1700
Size: 110 240 480 521 1492 187 2869
Fragments arranged by size:
2869 1492 521 480 240 187 110
BsrGI T'GTAC_A
Cuts at: 96 2622 96
Size: 2526 3373
BssHII G'CGCG_C
Cuts at: 1925 2449 2566 2608 2761 3024 3158 1925
Size: 524 117 42 153 263 134 4666
Fragments arranged by size:
4666 524 263 153 134 117 42
BssSI C'ACGA_G
Cuts at: 2135 3959 4971 2135
Size: 1824 1012 3063
Fragments arranged by size:
3063 1824 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2416
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2013 2114 2121 2262 2805 2874 513
Size: 552 948 101 7 141 543 69 3538
Fragments arranged by size:
3538 948 552 543 141 101 69 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2230 487
Size: 683 56 1004 4156
Fragments arranged by size:
4156 1004 683 56
BtsI GCAGTG_nn'
Cuts at: 2584 2929 3151 3415 4209 4229 2584
Size: 345 222 264 794 20 4254
Fragments arranged by size:
4254 794 345 264 222 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DraIII CAC_nnn'GTG
Cuts at: 2014 2014
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2102 4906 809
Size: 507 786 2804 1802
Fragments arranged by size:
2804 1802 786 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2259 2679
Size: 54 1425 50 275 18 222 207 420
Cuts at: 2679 2802 3153 3346 4235 5864 8
Size: 123 351 193 889 1629 43
Fragments arranged by size:
1629 1425 889 420 351 275 222 207
193 123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2802 3346 1537
Size: 275 990 544 4090
Fragments arranged by size:
4090 990 544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 2822 3829 1353
Size: 797 672 1007 3423
Fragments arranged by size:
3423 1007 797 672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2996 4486 4860 5006 387
Size: 1064 138 1407 1490 374 146 1280
Fragments arranged by size:
1490 1407 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2624 3962 4559 5346 5614 1239
Size: 393 992 1338 597 787 268 1524
Fragments arranged by size:
1524 1338 992 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3179 3179
Size: 5899
EcoO109I rG'GnC_Cy
Cuts at: 2833 2834 2870 3105 2833
Size: 1 36 235 5627
Fragments arranged by size:
5627 235 36 1
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 2085 2159 2483 2639 2736 2772 2867 5046
Size: 407 74 324 156 97 36 95 2179
Cuts at: 5046 1678
Size: 2531
Fragments arranged by size:
2531 2179 407 324 156 97 95 74
36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2911 2943 4540 4572 4614
Size: 32 135 32 1339 32 1597 32 42
Cuts at: 4614 4646 1373
Size: 32 2626
Fragments arranged by size:
2626 1597 1339 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2359 3243 3489 669
Size: 988 281 421 884 246 3079
Fragments arranged by size:
3079 988 884 421 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KasI G'GCGC_C
Cuts at: 2081 2768 2081
Size: 687 5212
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 2034 5775 2034
Size: 3741 2158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3019 5013 5197 1305
Size: 1714 1994 184 2007
Fragments arranged by size:
2007 1994 1714 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2414 3857 4216 4375 518
Size: 648 1101 147 1443 359 159 2042
Fragments arranged by size:
2042 1443 1101 648 359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2116 2317 2424 2653 2845 2876 2965 3315
Size: 510 201 107 229 192 31 89 350
Cuts at: 3315 3992 5140 5385 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 510 350 245 229 201
192 107 89 31
NaeI GCC'GGC
Cuts at: 3053 3053
Size: 5899
NarI GG'CG_CC
Cuts at: 2082 2769 2082
Size: 687 5212
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5347
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NgoMIV G'CCGG_C
Cuts at: 3051 3051
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 2938 3257 4802 1132
Size: 97 1709 319 1545 2229
Fragments arranged by size:
2229 1709 1545 319 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5899
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PmlI CAC'GTG
Cuts at: 2391 2391
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3105 3105
Size: 5899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2833 2869 2833
Size: 36 5863
PstI C_TGCA'G
Cuts at: 830 2579 2742 2862 3251 830
Size: 1749 163 120 389 3478
Fragments arranged by size:
3478 1749 389 163 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2845 2845
Size: 5899
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 5899
SacII CC_GC'GG
Cuts at: 2117 2877 2117
Size: 760 5139
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SanDI GG'GwC_CC
Cuts at: 3105 3105
Size: 5899
SapI GCTCTTCn'nnn_
Cuts at: 2150 2822 2150
Size: 672 5227
SbfI CC_TGCA'GG
Cuts at: 2742 3251 2742
Size: 509 5390
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2575 2738 2858 2941 3247 4382 5063
Size: 219 1530 163 120 83 306 1135 681
Cuts at: 5063 5254 826
Size: 191 1471
Fragments arranged by size:
1530 1471 1135 681 306 219 191 163
120 83
SfoI GGC'GCC
Cuts at: 2083 2770 2083
Size: 687 5212
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 2530 1700
Size: 830 5069
SmaI CCC'GGG
Cuts at: 1872 2073 2560 3226 1872
Size: 201 487 666 4545
Fragments arranged by size:
4545 666 487 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2915 4003 4904 5166 820
Size: 197 926 972 1088 901 262 1553
Fragments arranged by size:
1553 1088 972 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SrfI GCCC'GGGC
Cuts at: 2073 2073
Size: 5899
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 2585 3002 1517
Size: 1068 417 4414
Fragments arranged by size:
4414 1068 417
StyI C'CwwG_G
Cuts at: 513 1065 2781 3338 513
Size: 552 1716 557 3074
Fragments arranged by size:
3074 1716 557 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 2904 3926 3943 4096 4281 4726
Size: 1255 737 587 1022 17 153 185 445
Cuts at: 4726 325
Size: 1498
Fragments arranged by size:
1498 1255 1022 737 587 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 4145 5857
Size: 274 80 33 51 1443 645 1523 1712
Cuts at: 5857 96
Size: 138
Fragments arranged by size:
1712 1523 1443 645 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2921 2953 5859 5891 2921
Size: 32 2906 32 2929
Fragments arranged by size:
2929 2906 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2599 2011
Size: 588 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 2071 2558 3224 1870
Size: 201 487 666 4545
Fragments arranged by size:
4545 666 487 201
XmnI GAAnn'nnTTC
Cuts at: 4028 4028
Size: 5899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PspXI PsrI SacI SexAI
SfiI SwaI