Restriction map for
pFN21AE9548
(Circular) (Six-base) MAPSORT of: pFN21AE9548.seq Check: 8835 from: 1 to: 5899
pFN21AE9548 5899 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2736 2736
Size: 5899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2868 3242 1937
Size: 931 374 4594
Fragments arranged by size:
4594 931 374
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2049 3962 5346 5614 1632
Size: 417 1913 1384 268 1917
Fragments arranged by size:
1917 1913 1384 417 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 4798 5775 1203
Size: 3595 977 1327
Fragments arranged by size:
3595 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 5867
AlwNI CAG_nnn'CTG
Cuts at: 3642 5214 3642
Size: 1572 4327
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 3224 1870
Size: 73 1068 213 4545
Fragments arranged by size:
4545 1068 213 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2534 2711 3220 4675
Size: 325 217 627 282 465 177 509 1455
Cuts at: 4675 618
Size: 1842
Fragments arranged by size:
1842 1455 627 509 465 325 282 217
177
BanII G_rGCy'C
Cuts at: 1391 2900 3218 1391
Size: 1509 318 4072
Fragments arranged by size:
4072 1509 318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2571 2759 928
Size: 971 672 188 4068
Fragments arranged by size:
4068 971 672 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2095 2126 4510
Size: 107 122 71 631 605 423 31 2384
Cuts at: 4510 136
Size: 1525
Fragments arranged by size:
2384 1525 631 605 423 122 107 71
31
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2694 3960 5116 1790
Size: 282 622 1266 1156 2573
Fragments arranged by size:
2573 1266 1156 622 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 4588 442
Size: 416 53 83 399 24 2213 958 1753
Fragments arranged by size:
2213 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2698 4559 1239
Size: 1459 1861 2579
Fragments arranged by size:
2579 1861 1459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4024 4889 5187 4024
Size: 865 298 4736
Fragments arranged by size:
4736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3690 493
Size: 711 2486 2702
Fragments arranged by size:
2702 2486 711
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3127 3706 4088 4723
Size: 53 83 186 936 1594 579 382 635
Cuts at: 4723 275
Size: 1451
Fragments arranged by size:
1594 1451 936 635 579 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3059 3089 1178
Size: 30 165 30 1656 30 3988
Fragments arranged by size:
3988 1656 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2576 1186
Size: 1390 4509
BseYI C'CCAG_C
Cuts at: 2090 2441 3083 5102 5598 2090
Size: 351 642 2019 496 2391
Fragments arranged by size:
2391 2019 642 496 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2900 3960 4045 5116 2900
Size: 1060 85 1071 3683
Fragments arranged by size:
3683 1071 1060 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2694 2900 3218 3960 4045 5116
Size: 399 282 622 206 318 742 85 1071
Cuts at: 5116 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 622 399 318 282 206
85
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 2736 3254 844
Size: 1883 9 518 3489
Fragments arranged by size:
3489 1883 518 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2632 3786 1394
Size: 429 809 1154 3507
Fragments arranged by size:
3507 1154 809 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 4394 4568 65
Size: 1183 1134 2012 174 1396
Fragments arranged by size:
2012 1396 1183 1134 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4543 4730 1700
Size: 110 2733 187 2869
Fragments arranged by size:
2869 2733 187 110
BsrGI T'GTAC_A
Cuts at: 96 2907 96
Size: 2811 3088
BssHII G'CGCG_C
Cuts at: 1925 2683 1925
Size: 758 5141
BssSI C'ACGA_G
Cuts at: 2600 3040 3959 4971 2600
Size: 440 919 1012 3528
Fragments arranged by size:
3528 1012 919 440
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstXI CCAn_nnnn'nTGG
Cuts at: 2548 2764 2548
Size: 216 5683
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2523 2757 2981 513
Size: 552 939 60 459 234 224 3431
Fragments arranged by size:
3431 939 552 459 234 224 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 2694 3415 4209 4229 2694
Size: 721 794 20 4364
Fragments arranged by size:
4364 794 721 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DraIII CAC_nnn'GTG
Cuts at: 2544 2602 2544
Size: 58 5841
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2308 2403
Size: 54 1425 50 275 18 312 166 95
Cuts at: 2403 2889 3346 4235 5864 8
Size: 486 457 889 1629 43
Fragments arranged by size:
1629 1425 889 486 457 312 275 166
95 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 2885 3829 1353
Size: 989 543 944 3423
Fragments arranged by size:
3423 989 944 543
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2898 2898
Size: 5899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2698 3962 4559 5346 5614 1239
Size: 393 417 649 1264 597 787 268 1524
Fragments arranged by size:
1524 1264 787 649 597 417 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2010 2010
Size: 5899
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FspI TGC'GCA
Cuts at: 2198 4405 2198
Size: 2207 3692
FspAI rTGC'GCAy
Cuts at: 2198 2198
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 2234 5046 1678
Size: 556 2812 2531
Fragments arranged by size:
2812 2531 556
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4540 4572 4614 4646 1373
Size: 32 135 32 2968 32 42 32 2626
Fragments arranged by size:
2968 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3049 3243 3489 669
Size: 988 281 1111 194 246 3079
Fragments arranged by size:
3079 1111 988 281 246 194
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5013 5197 1305
Size: 3708 184 2007
Fragments arranged by size:
3708 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 2891 10
Size: 54 1425 343 573 486 3018
Fragments arranged by size:
3018 1425 573 486 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 3857 4216 4375 518
Size: 648 1185 1506 359 159 2042
Fragments arranged by size:
2042 1506 1185 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2571 2973 3315 3992 5140 5385 1606
Size: 488 477 402 342 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 488 477 402 342 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2523 2757 2981 513
Size: 552 939 519 234 224 3431
Fragments arranged by size:
3431 939 552 519 234 224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2602 2790 1108
Size: 1494 188 4217
Fragments arranged by size:
4217 1494 188
PfoI T'CCnGG_A
Cuts at: 2829 2829
Size: 5899
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3968 4000 5481 5513 3059
Size: 32 877 32 1481 32 3445
Fragments arranged by size:
3445 1481 877 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2010 2010
Size: 5899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspXI vC'TCGA_Gb
Cuts at: 3011 3011
Size: 5899
PstI C_TGCA'G
Cuts at: 830 2733 3251 830
Size: 1903 518 3478
Fragments arranged by size:
3478 1903 518
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2094 2094
Size: 5899
SacI G_AGCT'C
Cuts at: 2900 2900
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SbfI CC_TGCA'GG
Cuts at: 2733 3251 2733
Size: 518 5381
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2653 2729 3247 4382 5063 5254 826
Size: 219 1608 76 518 1135 681 191 1471
Fragments arranged by size:
1608 1471 1135 681 518 219 191 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3011 4003 4904 5166 820
Size: 197 926 1068 992 901 262 1553
Fragments arranged by size:
1553 1068 992 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2004 2448 2523 2757 2981 2997 3338
Size: 552 939 444 75 234 224 16 341
Cuts at: 3338 513
Size: 3074
Fragments arranged by size:
3074 939 552 444 341 234 224 75
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3007 3926 3943 4096 4281 4726 325
Size: 1255 1427 919 17 153 185 445 1498
Fragments arranged by size:
1498 1427 1255 919 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2907 4145 5857
Size: 274 80 33 51 1443 930 1238 1712
Cuts at: 5857 96
Size: 138
Fragments arranged by size:
1712 1443 1238 930 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 2122 2521 2545 2548 2764 2098
Size: 24 399 24 3 216 5233
Fragments arranged by size:
5233 399 216 24 24 3
XhoI C'TCGA_G
Cuts at: 1943 3011 1943
Size: 1068 4831
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 2336 4028 2336
Size: 1692 4207
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI